polyclonal goat anti feline igg  (Bio-Rad)

 
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  • 93
    Name:
    Goat F ab 2 anti Rat IgG H L
    Description:

    Catalog Number:
    305201
    Price:
    None
    Applications:
    Immunohistology, ELISA, Immunoblotting, Immunofluorescence
    Format:
    Purified
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    Structured Review

    Bio-Rad polyclonal goat anti feline igg

    https://www.bioz.com/result/polyclonal goat anti feline igg/product/Bio-Rad
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polyclonal goat anti feline igg - by Bioz Stars, 2020-09
    93/100 stars

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    Staining:

    Article Title: TARM1 is a novel LRC-encoded ITAM receptor that co-stimulates pro-inflammatory cytokine secretion by macrophages and neutrophils
    Article Snippet: .. Sections were sequentially stained with anti-TARM1 and goat F(ab’)2 anti-rat FITC (AbD Serotec) secondary Ab. ..

    Concentration Assay:

    Article Title: A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro
    Article Snippet: .. The membranes were blocked with 10% nonfat dry milk in TBS, 1% Tween-20 for 1 h at room temperature, and incubated with primary antibodies: goat polyclonal anti-HSV (AbD Serotec, Oxford, UK), mouse monoclonal anti-glycoprotein B (Santacruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-NOX4 (Santacruz Biotechnology, Dallas, TX, USA), or mouse monoclonal anti-tubulin (Sigma Aldrich, St. Louis, MO, USA) at a final concentration of 1 µg/mL. .. Secondary antibodies were horseradish peroxidase-conjugated (Jackson ImmunoResearch, West Grove, PA, USA).

    Incubation:

    Article Title: Collagen type XV and the ‘osteogenic status’
    Article Snippet: .. Cells were washed twice and incubated with 2.4 μg/ml of a polyclonal rabbit antimouse (DAKO Cytomation) or goat anti‐rat (AbD Serotec, Oxford, UK) immunoglobulins/FITC conjugate antibody at 4°C for 30 min. After two final washes, cells were analysed using a FACStar plus Cytometer (Becton Dickinson). ..

    Article Title: Membranous glomerulonephritis in the Iberian lynx (Lynx pardinus)
    Article Snippet: .. The primary antibodies used (all incubated at 4 °C overnight) were polyclonal rabbit anti-laminin (Dako® , Z0097, 1/100), polyclonal rabbit anti-fibronectin (Dako® , A0245, 1/500), monoclonal mouse anti-type IV collagen (Dako® , clone CIV 22, 1/30), polyclonal goat anti-feline IgA (Serotec® , AA125, 1/1000), polyclonal goat anti-feline IgM (Serotec® , AA124, 1/100) and polyclonal goat anti-feline IgG (Serotec® , 173 AA126, 1/1000). .. Biotinylated secondary antibodies swine anti-rabbit (for laminin and fibronectin, Dako® E0353, 1/200,), rabbit anti-goat (for IgA, IgG, and IgM, Pierce® , 31732, 1/200) and rabbit anti-mouse (for type IV collagen, Dako® E0465, 1/200) were used (incubated 30 min at RT) after washing.

    Article Title: Protection of dopaminergic cells by urate requires its accumulation in astrocytes
    Article Snippet: .. Membranes were incubated with horseradish peroxidase-conjugated rabbit-specific (1:2000; Pierce Biotechnology), mouse-specific (1:2000; Pierce Biotechnology; Rockford, IL) or goat-specific antibody (1:2000; Biorad Laboratories) and developed as above. .. Bands were acquired as JPG files; densitometric analysis was performed by ImageJ software (NIH).

    Article Title: Testicular biodistribution of 450 nm fluorescent latex particles after intramuscular injection in mice
    Article Snippet: .. After rinsing in PBST and then PBS, slides were incubated for 30 min with a polyclonal goat anti-rat secondary antibody (Adb Serotec, United Kingdom) conjugated with green fluorescent dyes (Dylight® 488) and diluted 1:200. ..

    Article Title: Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation
    Article Snippet: .. Coverslides were incubated with goat anti- E. coli polyclonal antibodies (dilution 1/100, AbD serotec) and Alexa 488-labeled anti-goat antibodies (dilution 1/300, Invitrogen). .. Actin cytoskeleton was stained using TRITC-labelled-phalloidin (Sigma).

    Article Title: A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro
    Article Snippet: .. The membranes were blocked with 10% nonfat dry milk in TBS, 1% Tween-20 for 1 h at room temperature, and incubated with primary antibodies: goat polyclonal anti-HSV (AbD Serotec, Oxford, UK), mouse monoclonal anti-glycoprotein B (Santacruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-NOX4 (Santacruz Biotechnology, Dallas, TX, USA), or mouse monoclonal anti-tubulin (Sigma Aldrich, St. Louis, MO, USA) at a final concentration of 1 µg/mL. .. Secondary antibodies were horseradish peroxidase-conjugated (Jackson ImmunoResearch, West Grove, PA, USA).

    Cytometry:

    Article Title: Collagen type XV and the ‘osteogenic status’
    Article Snippet: .. Cells were washed twice and incubated with 2.4 μg/ml of a polyclonal rabbit antimouse (DAKO Cytomation) or goat anti‐rat (AbD Serotec, Oxford, UK) immunoglobulins/FITC conjugate antibody at 4°C for 30 min. After two final washes, cells were analysed using a FACStar plus Cytometer (Becton Dickinson). ..

    Western Blot:

    Article Title: Cytotoxic Necrotizing Factor 1 and Hemolysin from Uropathogenic Escherichia coli Elicit Different Host Responses in the Murine Bladder
    Article Snippet: .. Western blot analysis of the CNF1 preparation was done with polyclonal goat anti-CNF , rabbit anti-goat IgG (H+L)-horseradish peroxidase (Bio-Rad), and an Amersham ECL Plus Western blotting detection system (GE Healthcare Biosciences, Piscataway, NJ). .. Bacterial endotoxin in the CNF1 preparation was detected with the Limulus amebocyte lysate (LAL) assay (Hycult Biotech) according to the manufacturer's directions.

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  • 93
    Bio-Rad polyclonal goat anti mzp3 igg
    Western immunoblot analysis of wild-type and mutated forms of <t>EC-mZP3.</t> Stably transfected cell lines were cultured overnight in serum-free medium, and concentrated culture medium was subjected to HPLC purification on a size-exclusion column. Fractions containing EC-mZP3 were identified by Western immunoblotting by using a goat <t>polyclonal</t> antiserum directed against mZP3 (anti-mZP3). These fractions were pooled, dialyzed, and quantitated by Western immunoassays and by spectrophotometry. Shown are HPLC-purified EC-mZP3 samples (≈200–300 ng) probed with anti-mZP3 and stained as described. Lanes: a, size standards; b, EC-mZP3-[wt]; c, EC-mZP3-[Ser-329]; d, EC-mZP3-[Ser-331]; e, EC-mZP3-[Ser-332]; f, EC-mZP3-[Ser-333]; g, EC-mZP3-[Ser-334]; h, EC-mZP3-[Ser-332–334]; i, EC-mZP3-[Ser-329–334]; and j, EC-mZP3-[Asn-330].
    Polyclonal Goat Anti Mzp3 Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal goat anti mzp3 igg/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal goat anti mzp3 igg - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Western immunoblot analysis of wild-type and mutated forms of EC-mZP3. Stably transfected cell lines were cultured overnight in serum-free medium, and concentrated culture medium was subjected to HPLC purification on a size-exclusion column. Fractions containing EC-mZP3 were identified by Western immunoblotting by using a goat polyclonal antiserum directed against mZP3 (anti-mZP3). These fractions were pooled, dialyzed, and quantitated by Western immunoassays and by spectrophotometry. Shown are HPLC-purified EC-mZP3 samples (≈200–300 ng) probed with anti-mZP3 and stained as described. Lanes: a, size standards; b, EC-mZP3-[wt]; c, EC-mZP3-[Ser-329]; d, EC-mZP3-[Ser-331]; e, EC-mZP3-[Ser-332]; f, EC-mZP3-[Ser-333]; g, EC-mZP3-[Ser-334]; h, EC-mZP3-[Ser-332–334]; i, EC-mZP3-[Ser-329–334]; and j, EC-mZP3-[Asn-330].

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Inactivation of the mouse sperm receptor, mZP3, by site-directed mutagenesis of individual serine residues located at the combining site for sperm

    doi:

    Figure Lengend Snippet: Western immunoblot analysis of wild-type and mutated forms of EC-mZP3. Stably transfected cell lines were cultured overnight in serum-free medium, and concentrated culture medium was subjected to HPLC purification on a size-exclusion column. Fractions containing EC-mZP3 were identified by Western immunoblotting by using a goat polyclonal antiserum directed against mZP3 (anti-mZP3). These fractions were pooled, dialyzed, and quantitated by Western immunoassays and by spectrophotometry. Shown are HPLC-purified EC-mZP3 samples (≈200–300 ng) probed with anti-mZP3 and stained as described. Lanes: a, size standards; b, EC-mZP3-[wt]; c, EC-mZP3-[Ser-329]; d, EC-mZP3-[Ser-331]; e, EC-mZP3-[Ser-332]; f, EC-mZP3-[Ser-333]; g, EC-mZP3-[Ser-334]; h, EC-mZP3-[Ser-332–334]; i, EC-mZP3-[Ser-329–334]; and j, EC-mZP3-[Asn-330].

    Article Snippet: Protein from lysates was subjected to SDS/PAGE and recombinant EC-mZP3 was detected by Western immunoblotting by using a polyclonal goat anti-mZP3 IgG (Pocono Rabbit Farms, Canadensis, PA), followed by a rabbit anti-goat IgG-alkaline phosphatase conjugate (Bio-Rad) and nitroblue tetrazolium chloride (NBT)/5-bromo-4-chloro-3-indolyphosphate p-toluidine salt (BCIP) (GIBCO/BRL), essentially as described ( ).

    Techniques: Western Blot, Stable Transfection, Transfection, Cell Culture, High Performance Liquid Chromatography, Purification, Spectrophotometry, Staining