rabbit polyclonal anti sequestosome  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit polyclonal anti sequestosome
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Rabbit Polyclonal Anti Sequestosome, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The interplay between serine proteases and caspase-1 regulates the autophagy-mediated secretion of Interleukin-1 beta in human neutrophils"

    Article Title: The interplay between serine proteases and caspase-1 regulates the autophagy-mediated secretion of Interleukin-1 beta in human neutrophils

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.832306

    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor p62/SQSTM1 evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Figure Legend Snippet: Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor p62/SQSTM1 evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.

    Techniques Used: Western Blot, Fluorescence, Immunostaining, Flow Cytometry, Staining

    rabbit polyclonal anti sequestosome  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit polyclonal anti sequestosome
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Rabbit Polyclonal Anti Sequestosome, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti sequestosome/product/NSJ Bioreagents
    Average 92 stars, based on 1 article reviews
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    rabbit polyclonal anti sequestosome - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "The interplay between serine proteases and caspase-1 regulates the autophagy-mediated secretion of Interleukin-1 beta in human neutrophils"

    Article Title: The interplay between serine proteases and caspase-1 regulates the autophagy-mediated secretion of Interleukin-1 beta in human neutrophils

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.832306

    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor p62/SQSTM1 evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Figure Legend Snippet: Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor p62/SQSTM1 evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.

    Techniques Used: Western Blot, Fluorescence, Immunostaining, Flow Cytometry, Staining

    anti prlr rabbit polyclonal  (NSJ Bioreagents)


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    NSJ Bioreagents anti prlr rabbit polyclonal
    Anti Prlr Rabbit Polyclonal, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti prlr rabbit polyclonal r31199  (NSJ Bioreagents)


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    NSJ Bioreagents anti prlr rabbit polyclonal r31199
    Anti Prlr Rabbit Polyclonal R31199, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal antibody  (NSJ Bioreagents)


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    NSJ Bioreagents polyclonal antibody
    Polyclonal Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat polyclonal antibody  (NSJ Bioreagents)


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    NSJ Bioreagents goat polyclonal antibody
    Goat Polyclonal Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal antibody  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit polyclonal antibody
    Rabbit Polyclonal Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal antibody 14 080  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit polyclonal antibody 14 080
    Rabbit Polyclonal Antibody 14 080, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal antibodies against type ii collagen  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit polyclonal antibodies against type ii collagen
    Rabbit Polyclonal Antibodies Against Type Ii Collagen, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human vegf polyclonal antibody  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit anti human vegf polyclonal antibody
    Rabbit Anti Human Vegf Polyclonal Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal antibody  (NSJ Bioreagents)


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    NSJ Bioreagents rabbit polyclonal antibody
    Rabbit Polyclonal Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NSJ Bioreagents rabbit polyclonal anti sequestosome
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Rabbit Polyclonal Anti Sequestosome, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NSJ Bioreagents anti prlr rabbit polyclonal
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Anti Prlr Rabbit Polyclonal, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NSJ Bioreagents anti prlr rabbit polyclonal r31199
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Anti Prlr Rabbit Polyclonal R31199, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NSJ Bioreagents polyclonal antibody
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
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    NSJ Bioreagents goat polyclonal antibody
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
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    NSJ Bioreagents rabbit polyclonal antibody
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
    Rabbit Polyclonal Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NSJ Bioreagents rabbit polyclonal antibody 14 080
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
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    NSJ Bioreagents rabbit polyclonal antibodies against type ii collagen
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
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    NSJ Bioreagents rabbit anti human vegf polyclonal antibody
    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor <t>p62/SQSTM1</t> evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.
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    Image Search Results


    Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor p62/SQSTM1 evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.

    Journal: Frontiers in Immunology

    Article Title: The interplay between serine proteases and caspase-1 regulates the autophagy-mediated secretion of Interleukin-1 beta in human neutrophils

    doi: 10.3389/fimmu.2022.832306

    Figure Lengend Snippet: Treatment of neutrophils with AEBSF inhibits autophagy induction. (A) Immunoblot analysis of LC3B in lysates of human neutrophils pre-treated or not with AEBSF (0.35 mM; t= -30 min), then stimulated or not with LPS (t= 0); treated or not with the autophagy flux blocker Baf A1 (t= 1 h), and with ATP (t= 2 h), assessed at 2 h and 15 min post-LPS stimulation. Immunoblot is representative of 2 independent experiments. Myeloperoxidase (MPO) was employed as loading control. (B) Median fluorescence intensity (MFI) levels of the autophagy receptor p62/SQSTM1 evaluated by immunostaining and flow cytometry at 2 h and 15 min post-LPS stimulation. Data represent the mean ± SEM of experiments performed with 4 donors (left) and representative histograms of one of these experiments (right). *p < 0.05. One-way ANOVA followed by Holm-Sidak’s multiple comparisons test. (C) Representative CLSM images of neutrophils pre-treated or not with AEBSF and stimulated or not with LPS+ATP for 4 h. Cells were stained with a specific antibody anti-LC3B and the corresponding secondary antibody and DNA was stained with ToPro-3. Images are representative of experiments with 3 different donors. (D) Quantification of the images of experiments performed with 3 donors as shown in (C) Data represent the fluorescence corresponding to cytosolic LC3B expressed in arbitrary units of fluorescence (AUF). *p<0.05. Kruskal-Wallis test.

    Article Snippet: Rabbit polyclonal antibody anti-microtubule-associated protein 1 light chain 3 beta (LC3B) cat. #sc28266; rabbit polyclonal antibody anti-IL-1 cat. #sc7884, anti-caspase-1 p10(C-20) cat. #515 antibody and cat. #sc-56036 were from Santa Cruz Biotechnology (Dallas, TX, USA); rabbit polyclonal anti-human IL-1 beta cat. NB600-633 was from Novus Biologicals (Co, USA); rabbit polyclonal anti-human-myeloperoxidase cat.#A0398 was from Dako (Glostrup, Denmark); rabbit polyclonal anti-sequestosome 1(SQSTM1)/p62 cat.# F48010 was from NSJ Bioreagents (San Diego, CA, USA); and purified mouse IgG1, κ isotype control cat. #555746 and rabbit polyclonal IgG was purchased from Jackson Immunoresearch.

    Techniques: Western Blot, Fluorescence, Immunostaining, Flow Cytometry, Staining