polyclonal antibody against bk ca  (Alomone Labs)


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    Alomone Labs polyclonal antibody against bk ca
    A) Immunoprecipitation (IP) of <t>BKCa</t> alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Polyclonal Antibody Against Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against bk ca/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody against bk ca - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria"

    Article Title: MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    Journal: The Journal of physiology

    doi: 10.1113/JP277769

    A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Figure Legend Snippet: A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Techniques Used: Immunoprecipitation, Western Blot, Activity Assay

    polyclonal antibody against bk ca  (Alomone Labs)


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    Alomone Labs polyclonal antibody against bk ca
    A) Immunoprecipitation (IP) of <t>BKCa</t> alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Polyclonal Antibody Against Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against bk ca/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody against bk ca - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria"

    Article Title: MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    Journal: The Journal of physiology

    doi: 10.1113/JP277769

    A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Figure Legend Snippet: A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Techniques Used: Immunoprecipitation, Western Blot, Activity Assay

    polyclonal antibody against bk ca  (Alomone Labs)


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    Structured Review

    Alomone Labs polyclonal antibody against bk ca
    A) Immunoprecipitation (IP) of <t>BKCa</t> alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Polyclonal Antibody Against Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against bk ca/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody against bk ca - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria"

    Article Title: MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    Journal: The Journal of physiology

    doi: 10.1113/JP277769

    A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Figure Legend Snippet: A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Techniques Used: Immunoprecipitation, Western Blot, Activity Assay

    polyclonal antibody against bk ca  (Alomone Labs)


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    Structured Review

    Alomone Labs polyclonal antibody against bk ca
    A) Immunoprecipitation (IP) of <t>BKCa</t> alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Polyclonal Antibody Against Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against bk ca/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody against bk ca - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria"

    Article Title: MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    Journal: The Journal of physiology

    doi: 10.1113/JP277769

    A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Figure Legend Snippet: A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Techniques Used: Immunoprecipitation, Western Blot, Activity Assay

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    • polyclonal antibody against bk ca  (Alomone Labs)
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    Alomone Labs polyclonal antibody against bk ca
    A) Immunoprecipitation (IP) of <t>BKCa</t> alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.
    Polyclonal Antibody Against Bk Ca, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against bk ca/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody against bk ca - by Bioz Stars, 2023-09
    93/100 stars
    		  Buy from Supplier

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    A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Journal: The Journal of physiology

    Article Title: MitoBK Ca channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    doi: 10.1113/JP277769

    Figure Lengend Snippet: A) Immunoprecipitation (IP) of BKCa alpha and BK-β1 subunit with an antibody against BKCa alpha (see methods). Western blots revealed the presence of GRIM 19 and SERCA as markers of mitochondrial and sarcoplasmic reticulum membranes (4A, lower panels). *The signal of BK-β1 in the whole lysate was detected with chemiluminescent (ECL) substrate Supersignal™ West Femto Maximun sensitivity (see methods). Taken together, these results indicates an association between mitoBKCa and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBKCa in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBKCa recorded from Wt mitoplasts. The Po of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca2+. C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBKCa channel activity with a Po of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca2+. D) Plots of I vs. V at 12 µM matrix Ca2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of Po vs. V for mitoBKCa channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Article Snippet: Polyclonal antibody against BK Ca (Rabbit Anti-KCNMA1 Cat. APC-021 and APC-151), and polyclonal antibody for BK-β1 subunit (Rabbit Anti-KCNMB1, Cat. APC-036) were from Alomone labs.

    Techniques: Immunoprecipitation, Western Blot, Activity Assay