polyclonal antibodies against herg  (Alomone Labs)


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    Alomone Labs polyclonal antibodies against herg
    Creation of Chinese hamster ovary (CHO) cell lines stably expressing <t>HERG</t> and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on <t>polyclonal</t> anti-KvLQT1 antiserum.
    Polyclonal Antibodies Against Herg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies against herg/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibodies against herg - by Bioz Stars, 2023-05
    93/100 stars

    Images

    1) Product Images from "Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines"

    Article Title: Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00479.2009

    Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.
    Figure Legend Snippet: Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.

    Techniques Used: Stable Transfection, Expressing, Western Blot

    Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.
    Figure Legend Snippet: Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.

    Techniques Used: Expressing, Immunostaining, Transfection, Stable Transfection, Plasmid Preparation, Staining, Incubation, FLAG-tag, Immunolabeling, Fluorescence, Software

    polyclonal antibodies against herg  (Alomone Labs)


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    Alomone Labs polyclonal antibodies against herg
    Creation of Chinese hamster ovary (CHO) cell lines stably expressing <t>HERG</t> and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on <t>polyclonal</t> anti-KvLQT1 antiserum.
    Polyclonal Antibodies Against Herg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies against herg/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibodies against herg - by Bioz Stars, 2023-05
    93/100 stars

    Images

    1) Product Images from "Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines"

    Article Title: Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00479.2009

    Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.
    Figure Legend Snippet: Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.

    Techniques Used: Stable Transfection, Expressing, Western Blot

    Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.
    Figure Legend Snippet: Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.

    Techniques Used: Expressing, Immunostaining, Transfection, Stable Transfection, Plasmid Preparation, Staining, Incubation, FLAG-tag, Immunolabeling, Fluorescence, Software

    polyclonal antibodies against herg  (Alomone Labs)


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    Alomone Labs polyclonal antibodies against herg
    Creation of Chinese hamster ovary (CHO) cell lines stably expressing <t>HERG</t> and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on <t>polyclonal</t> anti-KvLQT1 antiserum.
    Polyclonal Antibodies Against Herg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies against herg/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibodies against herg - by Bioz Stars, 2023-05
    86/100 stars

    Images

    1) Product Images from "Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines"

    Article Title: Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00479.2009

    Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.
    Figure Legend Snippet: Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.

    Techniques Used: Stable Transfection, Expressing, Western Blot

    Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.
    Figure Legend Snippet: Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.

    Techniques Used: Expressing, Immunostaining, Transfection, Stable Transfection, Plasmid Preparation, Staining, Incubation, FLAG-tag, Immunolabeling, Fluorescence, Software

    polyclonal antibodies against herg  (Alomone Labs)


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    Alomone Labs polyclonal antibodies against herg
    Creation of Chinese hamster ovary (CHO) cell lines stably expressing <t>HERG</t> and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on <t>polyclonal</t> anti-KvLQT1 antiserum.
    Polyclonal Antibodies Against Herg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies against herg/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibodies against herg - by Bioz Stars, 2023-05
    94/100 stars

    Images

    1) Product Images from "Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines"

    Article Title: Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00479.2009

    Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.
    Figure Legend Snippet: Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.

    Techniques Used: Stable Transfection, Expressing, Western Blot

    Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.
    Figure Legend Snippet: Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.

    Techniques Used: Expressing, Immunostaining, Transfection, Stable Transfection, Plasmid Preparation, Staining, Incubation, FLAG-tag, Immunolabeling, Fluorescence, Software

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    Alomone Labs polyclonal antibodies against herg
    Creation of Chinese hamster ovary (CHO) cell lines stably expressing <t>HERG</t> and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on <t>polyclonal</t> anti-KvLQT1 antiserum.
    Polyclonal Antibodies Against Herg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies against herg/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibodies against herg - by Bioz Stars, 2023-05
    93/100 stars
    		  Buy from Supplier

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    Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines

    doi: 10.1152/ajpheart.00479.2009

    Figure Lengend Snippet: Creation of Chinese hamster ovary (CHO) cell lines stably expressing HERG and KvLQT1-minK. Immunoblots of stable CHO cell lines expressing HERG or KvLQT1-minK1 are shown. Left: 25 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing Flag-HERG were subjected to Western blot analysis using anti-HERG antiserum. The core-glycosylated immature band of 135 kDa and the fully mature channel protein at 155 kDa are indicated by arrows. Right: 50 μg of membrane proteins from nontransfected CHO cells and a CHO clone stably expressing KvLQT1-minK were analyzed using an immunoblot based on polyclonal anti-KvLQT1 antiserum.

    Article Snippet: A monoclonal antibody against the Flag epitope (M2, Sigma) and polyclonal antibodies against HERG and KvLQT1 (Alomone Labs, Jerusalem, Israel) were used for immunoblot analysis.

    Techniques: Stable Transfection, Expressing, Western Blot

    Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Pore mutants of HERG and KvLQT1 downregulate the reciprocal currents in stable cell lines

    doi: 10.1152/ajpheart.00479.2009

    Figure Lengend Snippet: Cell surface expression of HERG. A: immunostaining analysis of transfected CHO cells. A CHO cell line stably expressing hemagluttinin (HA)-tagged HERG was transiently transfected with an expression vector for Flag-KvLQT1 (left) or Flag-Kir2.1 (right). Two days after transfection, cells were fixed with paraformaldehyde and probed with anti-HA monoclonal antibody for surface HERG channel staining. After unbound antibodies were washed out, cells were permeabilized and incubated with polyclonal antibody against the Flag epitope. CHO cells were immunolabeled with anti-HA (a and d) or anti-Flag (b and e) using Alexa fluor 488-conjugated goat anti-mouse and Alexa fluor 594-conjugated goat anti-rabbit secondary antibodies, respectively. Merged patterns of a and b are shown in c; merged patterns of d and e are shown in f. B: fluorescence intensity measurements. Elements software was used to semiquantitate green and red fluorescence signals of Flag-KvLQT1- and Flag-Kir2.1-positive cells. Fluorescence intensity signals (means ± SE) for KvLQT1-transfected (n = 12) and Kir2.1-transfected (n = 8) cells are shown.

    Article Snippet: A monoclonal antibody against the Flag epitope (M2, Sigma) and polyclonal antibodies against HERG and KvLQT1 (Alomone Labs, Jerusalem, Israel) were used for immunoblot analysis.

    Techniques: Expressing, Immunostaining, Transfection, Stable Transfection, Plasmid Preparation, Staining, Incubation, FLAG-tag, Immunolabeling, Fluorescence, Software