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Santa Cruz Biotechnology rabbit igg anti caga polyclonal antibody b 300
Expression, delivery, and phosphorylation of <t>CagA.</t> (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the <t>polyclonal</t> anti-CagA antibody b-300 (bottom). (B)
Rabbit Igg Anti Caga Polyclonal Antibody B 300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg anti caga polyclonal antibody b 300/product/Santa Cruz Biotechnology
Average 85 stars, based on 1263 article reviews
Price from $9.99 to $1999.99
rabbit igg anti caga polyclonal antibody b 300 - by Bioz Stars, 2020-09
85/100 stars

Images

1) Product Images from "Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿ §"

Article Title: Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿ §

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.02330-08

Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)
Figure Legend Snippet: Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)

Techniques Used: Expressing, Western Blot

2) Product Images from "Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori"

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

Journal: PLoS ONE

doi: 10.1371/journal.pone.0150061

Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
Figure Legend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

Techniques Used: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight

3) Product Images from "Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori"

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

Journal: PLoS ONE

doi: 10.1371/journal.pone.0150061

Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
Figure Legend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

Techniques Used: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight

4) Product Images from "Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori"

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

Journal: PLoS ONE

doi: 10.1371/journal.pone.0150061

Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
Figure Legend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

Techniques Used: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight

5) Product Images from "Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori"

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

Journal: PLoS ONE

doi: 10.1371/journal.pone.0150061

Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
Figure Legend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

Techniques Used: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight

6) Product Images from "Caveolin-1 Protects B6129 Mice against Helicobacter pylori Gastritis"

Article Title: Caveolin-1 Protects B6129 Mice against Helicobacter pylori Gastritis

Journal: PLoS Pathogens

doi: 10.1371/journal.ppat.1003251

Loss of Cav1 promotes recruitment of macrophages to stomachs infected with H. pylori SS1. (A) Differential expression of mRNAs in mouse gastric tissue upon an 11-month infection with H. pylori strain SS1. CT-values from RT-qPCRs on total RNA extracted from resected stomachs were normalized to beta-2-microglobulin (b2M) and are presented as mean ± S.E. (n = 15 per group); *p
Figure Legend Snippet: Loss of Cav1 promotes recruitment of macrophages to stomachs infected with H. pylori SS1. (A) Differential expression of mRNAs in mouse gastric tissue upon an 11-month infection with H. pylori strain SS1. CT-values from RT-qPCRs on total RNA extracted from resected stomachs were normalized to beta-2-microglobulin (b2M) and are presented as mean ± S.E. (n = 15 per group); *p

Techniques Used: Infection, Expressing

7) Product Images from "Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori"

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

Journal: PLoS ONE

doi: 10.1371/journal.pone.0150061

Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
Figure Legend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

Techniques Used: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight

8) Product Images from "Dynamic Expansion and Contraction of cagA Copy Number in Helicobacter pylori Impact Development of Gastric Disease"

Article Title: Dynamic Expansion and Contraction of cagA Copy Number in Helicobacter pylori Impact Development of Gastric Disease

Journal: mBio

doi: 10.1128/mBio.01779-16

Relative levels of CagA protein and CagA phosphorylation. (A) The protein levels of CagA and UreA in lysates of H. pylori strains PMSS1, S F -1, S L -2, M F -3, and M L -1 were measured by Western blotting (upper panel). For each lysate, 0.5, 1, 1.5, and 2 μg of total protein were used to determine standard curves for CagA and UreA. The immunoblot images were analyzed using ImageJ software, and the values were plotted on a graph (lower panel). (B) Ratios of CagA to UreA were calculated, and each value was normalized to the value calculated for cagA -S F -1 to determine relative CagA protein levels. The bar graphs indicate average levels of CagA expression of each strain, and error bars represent standard deviations, derived from results of 2 independent experiments. (C) Lysates of AGS cells that were infected with H. pylori strains PMSS1, Δ cagA FL -2, S F -1, S L -2, M F -3, and M L -1 were immunoblotted for phosphorylated CagA (p-CagA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), CagA, and UreA. GAPDH and UreA were used as controls.
Figure Legend Snippet: Relative levels of CagA protein and CagA phosphorylation. (A) The protein levels of CagA and UreA in lysates of H. pylori strains PMSS1, S F -1, S L -2, M F -3, and M L -1 were measured by Western blotting (upper panel). For each lysate, 0.5, 1, 1.5, and 2 μg of total protein were used to determine standard curves for CagA and UreA. The immunoblot images were analyzed using ImageJ software, and the values were plotted on a graph (lower panel). (B) Ratios of CagA to UreA were calculated, and each value was normalized to the value calculated for cagA -S F -1 to determine relative CagA protein levels. The bar graphs indicate average levels of CagA expression of each strain, and error bars represent standard deviations, derived from results of 2 independent experiments. (C) Lysates of AGS cells that were infected with H. pylori strains PMSS1, Δ cagA FL -2, S F -1, S L -2, M F -3, and M L -1 were immunoblotted for phosphorylated CagA (p-CagA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), CagA, and UreA. GAPDH and UreA were used as controls.

Techniques Used: Western Blot, Software, Expressing, Derivative Assay, Infection

9) Product Images from "Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori"

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

Journal: PLoS ONE

doi: 10.1371/journal.pone.0150061

Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
Figure Legend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

Techniques Used: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight

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    Santa Cruz Biotechnology rabbit igg anti caga polyclonal antibody b 300
    Expression, delivery, and phosphorylation of <t>CagA.</t> (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the <t>polyclonal</t> anti-CagA antibody b-300 (bottom). (B)
    Rabbit Igg Anti Caga Polyclonal Antibody B 300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit igg anti caga polyclonal antibody b 300/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit igg anti caga polyclonal antibody b 300 - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    90
    Santa Cruz Biotechnology polyclonal anti caga antibody b 300
    Analysis of the <t>CagA</t> protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using <t>polyclonal</t> anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.
    Polyclonal Anti Caga Antibody B 300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti caga antibody b 300/product/Santa Cruz Biotechnology
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    polyclonal anti caga antibody b 300 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)

    Journal: Journal of Clinical Microbiology

    Article Title: Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿ §

    doi: 10.1128/JCM.02330-08

    Figure Lengend Snippet: Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)

    Article Snippet: Alternatively, membranes were probed with a 1:5,000 dilution of rabbit IgG anti-CagA polyclonal antibody b-300 (Santa Cruz Biotechnology), followed by a 1:20,000 dilution of HRP-conjugated bovine anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology).

    Techniques: Expressing, Western Blot

    Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

    Journal: PLoS ONE

    Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori

    doi: 10.1371/journal.pone.0150061

    Figure Lengend Snippet: Analysis of the CagA protein patterns by immunoblot analysis. (A) Lysates of H . pylori isolated from the tissue specimens of various gastric patients were prepared as described in the Materials and Methods. Aliquots of protein (20 μg) were separated by SDS-gel electrophoresis and CagA was detected by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz). The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression. The numbers at the top identify the various H . pylori colonies, while those on the left indicate the molecular weight markers in kDa. It can be seen that there are four types of CagA fragmentation patterns. (B) The percentages of ABD subtypes based on the CagA protein fragmentation patterns across the 96 H . pylori cagA ABD colonies that were analyzed and clearly ABD-2 is the predominant type.

    Article Snippet: Proteins (20 μg) were separated by SDS-gel electrophoresis, amounts of CagA and phosphorylated-CagA were determined by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz) and anti-phosphotyrosine antibody PY99 (Santa Cruz).

    Techniques: Isolation, SDS-Gel, Electrophoresis, Knock-Out, Mutagenesis, Negative Control, Expressing, Molecular Weight