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polyacrylamide substrates  (TargetMol)


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    TargetMol polyacrylamide substrates
    Polyacrylamide Substrates, supplied by TargetMol, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyacrylamide+substrates/Polyacrylamide/pm37659729-70-4-40
    Average 91 stars, based on 1 article reviews
    polyacrylamide substrates - by Bioz Stars, 2026-09
    91/100 stars

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    Cell Culture:

    Article Title: Substrate nesting guides cyst morphogenesis of human pluripotent stem cells without 3D extracellular matrix overlay.
    Article Snippet: .. Before plating cells onto polyacrylamide substrates, the gels ere allowed to recover to room temperature for 1 h. Cultured PSCs were dissociated using Accutase (Sigma A6964) at 37 °C for min, centrifuged and resuspended in mTeSR1 containing 10 μM 27632 (Targetmol). ..



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    Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated polyacrylamide gel substrates. (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

    Journal: FEBS Open Bio

    Article Title: DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanisms

    doi: 10.1002/2211-5463.13798

    Figure Lengend Snippet: Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated polyacrylamide gel substrates. (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

    Article Snippet: Polyacrylamide gel substrates were prepared through the polymerization of acrylamide (Bio‐Rad, Waltham, MA, USA, Cat. No. 1610140) and bis‐acrylamide, with varying concentrations to achieve the desired stiffness levels (Table ).

    Techniques: Cell Culture, RNA Sequencing, Incubation, RNA Extraction, Sequencing