Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Free reactive oxygen species affect basal and LPS induced gene expression on lipid modified matrices. A Bar charts represent relative gene expression of TNFA and IL1A normalized to B2M showing mean ± SD, n = 4. THP-1 cells were activated with 15 nM PMA, cultured on modified matrices for 24 h, and then stimulated for 2 h with human TNFα (100 ng/ml), Flagellin (0.25 μg/ml, TLR5), FSL-1 (0.5 μg/ml, TLR2, TLR6), and Poly(I:C) (20 μg/ml TLR3), respectively. B, C Bar charts show relative expression levels of macrophages treated with 5 mM N-Acetylcysteine (NAC) immediately after seeding and cultured for 24 h, followed by stimulation with 500 ng/ml LPS for 2 h, mean ± SD, n = 4. Asterisks indicate statistical significance (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001), and exact p-values are shown for borderline significance determined by one-way ANOVA.

Article Snippet: In detail, human TNFα (InvitrogenThermo Fisher, USA) was added to a final concentration of 100 ng/ml, 0.25 μg/ml of Flagellin (TLR5, InvivoGen, France), 0.5 μg/ml of FSL-1 (TLR2, TLR6, InvivoGen) and 20 μg/ml of Poly(I:C) (TLR3, InvivoGen) [ , ].

Techniques: Gene Expression, Modification, Cell Culture, Expressing