pmsf ripa (Beyotime)
98
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Name:
PMSF 100mM
Description:
Catalog Number:
ST506
Price:
108.0
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Structured Review
Beyotime
pmsf ripa
https://www.bioz.com/result/pmsf ripa/product/Beyotime
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/pmsf ripa/product/Beyotime
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pmsf ripa - by Bioz Stars,
2021-04
98/100 stars
Images
1) Product Images from "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT"
Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
doi: 10.1155/2018/2305683
Figure Legend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
Techniques Used: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot
2) Product Images from "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT"
Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
doi: 10.1155/2018/2305683
Figure Legend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
Techniques Used: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot
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P0013C; Beyotime) supplemented with the Infection:Article Title: GADD45γ Activated Early in the Course of Herpes Simplex Virus 1 Infection Suppresses the Activation of a Network of Innate Immunity Genes Article Snippet: The assays were performed on a Step One Plus system (Applied Biosystems), and results were analyzed with software provided by the supplier. .. The cells were harvested at the indicated times after infection by scraping into their medium, rinsed with phosphate-buffered saline (PBS), solubilized in RIPA buffer (product no. P0013C; Beyotime) supplemented with the |