pmsf ripa  (Beyotime)

 
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    Name:
    PMSF 100mM
    Description:

    Catalog Number:
    ST506
    Price:
    108.0
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    Beyotime pmsf ripa
    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% <t>PMSF-RIPA</t> and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    https://www.bioz.com/result/pmsf ripa/product/Beyotime
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf ripa - by Bioz Stars, 2021-04
    98/100 stars

    Images

    1) Product Images from "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT"

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2018/2305683

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
    Figure Legend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Techniques Used: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot

    2) Product Images from "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT"

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2018/2305683

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
    Figure Legend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Techniques Used: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot

    Related Articles

    Protein Extraction:

    Article Title: Necrostatin-1 protects mice from acute lung injury by suppressing necroptosis and reactive oxygen species
    Article Snippet: .. Protein extraction and western blotting Snap-frozen lung tissues or cells were homogenized and resuspended in lysis buffer (cat. no. P0013B; Beyotime Institute of Biotechnology) containing 1% phenylmethylsulfonyl fluoride (cat. no. ST506; Beyotime Institute of Biotechnology) for 30 min at 4°C. .. Protein concentrations were determined using a bicinchoninic acid (BCA) assay kit (cat. no. P0012; Beyotime Institute of Biotechnology) at 37°C.

    Western Blot:

    Article Title: Necrostatin-1 protects mice from acute lung injury by suppressing necroptosis and reactive oxygen species
    Article Snippet: .. Protein extraction and western blotting Snap-frozen lung tissues or cells were homogenized and resuspended in lysis buffer (cat. no. P0013B; Beyotime Institute of Biotechnology) containing 1% phenylmethylsulfonyl fluoride (cat. no. ST506; Beyotime Institute of Biotechnology) for 30 min at 4°C. .. Protein concentrations were determined using a bicinchoninic acid (BCA) assay kit (cat. no. P0012; Beyotime Institute of Biotechnology) at 37°C.

    Article Title: Tandem Mass Tag-based quantitative proteomics analysis of metabolic associated fatty liver disease induced by high fat diet in mice
    Article Snippet: The relative expression of mRNA was calculated using the 2−ΔΔCt method. .. Verification of proteins by western blotAbout 100 mg of liver tissues were homogenized in 1 ml of Radio-Immunoprecipitation Assay lysis buffer (P0013B, Beyotime Biotechnology, China) and phenylmethanesulfonyl fluoride (ST506, Beyotime Biotechnology, China) with an automatic grinder (JXFSTPRP024, Shanghai Jingxin Industrial development Co., Ltd, China), and the lysates were centrifuged at 14,000 rpm for 15 min at 4 °C. .. The total protein concentration in the supernatant was quantified using a BCA protein assay kit (Beyotime Biotechnology, China).

    Article Title: Alterations of autophagy in knee cartilage by treatment with treadmill exercise in a rat osteoarthritis model
    Article Snippet: .. Western blotting Following removal from storage at −80°C, the cartilage was washed in PBS twice, and ultrasonication was performed in radioimmunoprecipitation assay lysis buffer (cat. no. P0013C) with 1 mM phenylmethylsulfonyl fluoride (cat. no. ST506) (both from Beyotime Institute of Biotechnology). .. The lysates were centrifuged at 14,000 × g for 5 min at 4°C, and the supernatants were obtained to measure the protein concentration with the BCA assay kit.

    Lysis:

    Article Title: Necrostatin-1 protects mice from acute lung injury by suppressing necroptosis and reactive oxygen species
    Article Snippet: .. Protein extraction and western blotting Snap-frozen lung tissues or cells were homogenized and resuspended in lysis buffer (cat. no. P0013B; Beyotime Institute of Biotechnology) containing 1% phenylmethylsulfonyl fluoride (cat. no. ST506; Beyotime Institute of Biotechnology) for 30 min at 4°C. .. Protein concentrations were determined using a bicinchoninic acid (BCA) assay kit (cat. no. P0012; Beyotime Institute of Biotechnology) at 37°C.

    Article Title: Tandem Mass Tag-based quantitative proteomics analysis of metabolic associated fatty liver disease induced by high fat diet in mice
    Article Snippet: The relative expression of mRNA was calculated using the 2−ΔΔCt method. .. Verification of proteins by western blotAbout 100 mg of liver tissues were homogenized in 1 ml of Radio-Immunoprecipitation Assay lysis buffer (P0013B, Beyotime Biotechnology, China) and phenylmethanesulfonyl fluoride (ST506, Beyotime Biotechnology, China) with an automatic grinder (JXFSTPRP024, Shanghai Jingxin Industrial development Co., Ltd, China), and the lysates were centrifuged at 14,000 rpm for 15 min at 4 °C. .. The total protein concentration in the supernatant was quantified using a BCA protein assay kit (Beyotime Biotechnology, China).

    Article Title: Alterations of autophagy in knee cartilage by treatment with treadmill exercise in a rat osteoarthritis model
    Article Snippet: .. Western blotting Following removal from storage at −80°C, the cartilage was washed in PBS twice, and ultrasonication was performed in radioimmunoprecipitation assay lysis buffer (cat. no. P0013C) with 1 mM phenylmethylsulfonyl fluoride (cat. no. ST506) (both from Beyotime Institute of Biotechnology). .. The lysates were centrifuged at 14,000 × g for 5 min at 4°C, and the supernatants were obtained to measure the protein concentration with the BCA assay kit.

    Radio Immunoprecipitation:

    Article Title: Tandem Mass Tag-based quantitative proteomics analysis of metabolic associated fatty liver disease induced by high fat diet in mice
    Article Snippet: The relative expression of mRNA was calculated using the 2−ΔΔCt method. .. Verification of proteins by western blotAbout 100 mg of liver tissues were homogenized in 1 ml of Radio-Immunoprecipitation Assay lysis buffer (P0013B, Beyotime Biotechnology, China) and phenylmethanesulfonyl fluoride (ST506, Beyotime Biotechnology, China) with an automatic grinder (JXFSTPRP024, Shanghai Jingxin Industrial development Co., Ltd, China), and the lysates were centrifuged at 14,000 rpm for 15 min at 4 °C. .. The total protein concentration in the supernatant was quantified using a BCA protein assay kit (Beyotime Biotechnology, China).

    Article Title: Alterations of autophagy in knee cartilage by treatment with treadmill exercise in a rat osteoarthritis model
    Article Snippet: .. Western blotting Following removal from storage at −80°C, the cartilage was washed in PBS twice, and ultrasonication was performed in radioimmunoprecipitation assay lysis buffer (cat. no. P0013C) with 1 mM phenylmethylsulfonyl fluoride (cat. no. ST506) (both from Beyotime Institute of Biotechnology). .. The lysates were centrifuged at 14,000 × g for 5 min at 4°C, and the supernatants were obtained to measure the protein concentration with the BCA assay kit.

    Sonication:

    Article Title: RiPerC Attenuates Cerebral Ischemia Injury through Regulation of miR-98/PIK3IP1/PI3K/AKT Signaling Pathway
    Article Snippet: .. In brief, brain tissues were homogenized in RIPA buffer (P1003B, Beyotime, Wuhan, China) containing PMSF (ST506, Beyotime, Wuhan, China) and then sonicated on ice. .. After centrifugation, the supernatant was collected for Western blot assay.

    Article Title: GADD45γ Activated Early in the Course of Herpes Simplex Virus 1 Infection Suppresses the Activation of a Network of Innate Immunity Genes
    Article Snippet: The assays were performed on a Step One Plus system (Applied Biosystems), and results were analyzed with software provided by the supplier. .. The cells were harvested at the indicated times after infection by scraping into their medium, rinsed with phosphate-buffered saline (PBS), solubilized in RIPA buffer (product no. P0013C; Beyotime) supplemented with the protease inhibitor phenylmethylsulfonyl fluoride (PMSF; product no. ST506; Beyotime) as specified by the manufacturer, and briefly sonicated. ..

    Protease Inhibitor:

    Article Title: High autophagic flux guards ESC identity through coordinating autophagy machinery gene program by FOXO1
    Article Snippet: .. Cells were lysed on ice in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.5% sodium deoxycholate, 1% Nonidet P-40, 5 mM EGTA, 2 mM EDTA, 10 mM NaF) for 30 min and a protease inhibitor cocktail (04693116001, Roche, Mannheim, Germany) and 1 mM PMSF (ST506, Beyotime, Shanghai, China) was added. .. The lysates were mixed with loading buffer, boiled, centrifuged, and subjected to SDS-PAGE electrophoresis before transfer to PVDF membranes (Millipore).

    Article Title: GADD45γ Activated Early in the Course of Herpes Simplex Virus 1 Infection Suppresses the Activation of a Network of Innate Immunity Genes
    Article Snippet: The assays were performed on a Step One Plus system (Applied Biosystems), and results were analyzed with software provided by the supplier. .. The cells were harvested at the indicated times after infection by scraping into their medium, rinsed with phosphate-buffered saline (PBS), solubilized in RIPA buffer (product no. P0013C; Beyotime) supplemented with the protease inhibitor phenylmethylsulfonyl fluoride (PMSF; product no. ST506; Beyotime) as specified by the manufacturer, and briefly sonicated. ..

    Infection:

    Article Title: GADD45γ Activated Early in the Course of Herpes Simplex Virus 1 Infection Suppresses the Activation of a Network of Innate Immunity Genes
    Article Snippet: The assays were performed on a Step One Plus system (Applied Biosystems), and results were analyzed with software provided by the supplier. .. The cells were harvested at the indicated times after infection by scraping into their medium, rinsed with phosphate-buffered saline (PBS), solubilized in RIPA buffer (product no. P0013C; Beyotime) supplemented with the protease inhibitor phenylmethylsulfonyl fluoride (PMSF; product no. ST506; Beyotime) as specified by the manufacturer, and briefly sonicated. ..

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  • 98
    Beyotime pmsf ripa
    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% <t>PMSF-RIPA</t> and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
    Pmsf Ripa, supplied by Beyotime, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf ripa/product/Beyotime
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf ripa - by Bioz Stars, 2021-04
    98/100 stars
      Buy from Supplier

    98
    Beyotime pmsf
    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% <t>PMSF-RIPA</t> and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
    Pmsf, supplied by Beyotime, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Beyotime
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-04
    98/100 stars
      Buy from Supplier

    Image Search Results


    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    doi: 10.1155/2018/2305683

    Figure Lengend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Article Snippet: Western Blotting Assay Total protein product was extracted by incubating cells with 1% PMSF-RIPA (Catl. number ST506, Catl. number P0013B, Beyotime Biotechnology, China) and then centrifuged at 12000 rpm for 10 min. β -Actin was selected as the internal reference protein.

    Techniques: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    doi: 10.1155/2018/2305683

    Figure Lengend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Article Snippet: Total protein product was extracted by incubating cells with 1% PMSF-RIPA (Catl. number ST506, Catl. number P0013B, Beyotime Biotechnology, China) and then centrifuged at 12000 rpm for 10 min. β -Actin was selected as the internal reference protein.

    Techniques: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot