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TaKaRa pmat1552 pyrf te 04
( A , B ) Expression of TE-01 , TE-02 , TE-03 , and <t>TE-04</t> genes. ( A ) Structure of plasmids <t>pMAT1552-pyrF-TE-01,</t> pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.
Pmat1552 Pyrf Te 04, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pmat1552 pyrf te 04 - by Bioz Stars, 2020-08
93/100 stars

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1) Product Images from "Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides"

Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms20030786

( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.
Figure Legend Snippet: ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

Techniques Used: Expressing, Polymerase Chain Reaction, Amplification

Related Articles

Polymerase Chain Reaction:

Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides
Article Snippet: .. The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA). ..

Clone Assay:

Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides
Article Snippet: .. The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA). ..

Recombinant:

Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides
Article Snippet: .. The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA). ..

Plasmid Preparation:

Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides
Article Snippet: .. The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA). ..

Construct:

Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides
Article Snippet: .. The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA). ..

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    TaKaRa pmat1552 pyrf te 04
    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and <t>TE-04</t> genes. ( A ) Structure of plasmids <t>pMAT1552-pyrF-TE-01,</t> pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.
    Pmat1552 Pyrf Te 04, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmat1552 pyrf te 04/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmat1552 pyrf te 04 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

    Journal: International Journal of Molecular Sciences

    Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides

    doi: 10.3390/ijms20030786

    Figure Lengend Snippet: ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

    Article Snippet: The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA).

    Techniques: Expressing, Polymerase Chain Reaction, Amplification