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Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog <t>PM1</t> microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and
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Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog <t>PM1</t> microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and
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Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog <t>PM1</t> microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and
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Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog PM1 microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and

Journal: Microbial Ecology

Article Title: Dissolved Organic Carbon Regulates Bacterial Ingestion by Tetraselmis sp.

doi: 10.1007/s00248-026-02752-z

Figure Lengend Snippet: Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog PM1 microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and

Article Snippet: Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and .Although Biolog PM1 plates were originally developed for bacterial metabolic profiling, they have increasingly been applied to microalgae to assess heterotrophic carbon use [ , ].

Techniques: Activity Assay, Control, Algae, Bacteria

Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog PM1 microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and

Journal: Microbial Ecology

Article Title: Dissolved Organic Carbon Regulates Bacterial Ingestion by Tetraselmis sp.

doi: 10.1007/s00248-026-02752-z

Figure Lengend Snippet: Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog PM1 microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and

Article Snippet: Biolog PM1 carbon source plates were used to assess organic carbon utilization by Tetraselmis sp. A “bacteria-only” control was prepared by filtering cultures through a 0.8 μm polycarbonate membrane.

Techniques: Activity Assay, Control, Algae, Bacteria

Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog PM1 microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and

Journal: Microbial Ecology

Article Title: Dissolved Organic Carbon Regulates Bacterial Ingestion by Tetraselmis sp.

doi: 10.1007/s00248-026-02752-z

Figure Lengend Snippet: Carbon metabolic activity of Tetraselmis sp. under low-nutrient conditions measured using Biolog PM1 microplates. Metabolic responses to 95 different carbon substrates and one control were assessed under ( a ) low-light and ( b ) dark conditions. Values represent the “algae + bacteria” treatment after subtraction of the “bacteria-only” control, providing an estimate of algal carbon metabolic capacity. Under low-light conditions ( a ), the top five utilized substrates were α-D-glucose (0.475, C9), glycyl-L-aspartic acid (0.460, F1), L-glutamine (0.323, E1), propionic acid (0.294, F7), and succinic acid (0.290, A5). Under dark conditions ( b ), the top five substrates were pyruvic acid (1.172, H8), L-glutamic acid (1.166, B12), adenosine (1.044, E12), L-serine (0.940, G3), and maltotriose (0.824, E10). Individual carbon metabolic activity and corrected maximum daily growth rate (NRmax) for the “algae + bacteria” and “bacteria-only” treatments are shown in Figures and

Article Snippet: Biolog PM1 microplates were used to assess organic carbon utilization under low-light and dark conditions.

Techniques: Activity Assay, Control, Algae, Bacteria