random hexamer primers  (Jena Bioscience)


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  • 93
    Name:
    Random Hexamers
    Description:

    Catalog Number:
    PM-301L
    Price:
    108.5
    Category:
    Molecular Biology
    Size:
    1 ml
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    Structured Review

    Jena Bioscience random hexamer primers

    https://www.bioz.com/result/random hexamer primers/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    random hexamer primers - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Lysis:

    Article Title: Interferon-α Revisited: Individualized Treatment Management Eased the Selective Pressure of Tyrosine Kinase Inhibitors on BCR-ABL1 Mutations Resulting in a Molecular Response in High-Risk CML Patients
    Article Snippet: .. Total RNA was prepared from the total leukocytes of peripheral blood by Trizol (Thermofisher scientific, Waltham, MA, USA) lysis-phenol-chloroform extraction; cDNA was synthesized by M-MLV reverse transcriptase (Promega Corporation, Madison, WI, USA) using random hexamer primers (Jena Bioscience, Jena, Germany). .. GUSB (in Prague lab) or ABL1 (in Olomouc lab) were used as the control genes for the standardized quantification of BCR-ABL1 mRNA using reverse-transcriptase qPCR.

    Synthesized:

    Article Title: Interferon-α Revisited: Individualized Treatment Management Eased the Selective Pressure of Tyrosine Kinase Inhibitors on BCR-ABL1 Mutations Resulting in a Molecular Response in High-Risk CML Patients
    Article Snippet: .. Total RNA was prepared from the total leukocytes of peripheral blood by Trizol (Thermofisher scientific, Waltham, MA, USA) lysis-phenol-chloroform extraction; cDNA was synthesized by M-MLV reverse transcriptase (Promega Corporation, Madison, WI, USA) using random hexamer primers (Jena Bioscience, Jena, Germany). .. GUSB (in Prague lab) or ABL1 (in Olomouc lab) were used as the control genes for the standardized quantification of BCR-ABL1 mRNA using reverse-transcriptase qPCR.

    Article Title: Proteomic analysis of imatinib-resistant CML-T1 cells reveals calcium homeostasis as a potential therapeutic target
    Article Snippet: Wnt target gene microarray Total RNA was isolated and purified from the CML-T1 and the CML-T1/IR cells with TRIzol reagent (Thermo Fisher Scientific, Inc.) and an RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. The RNA quality and quantity were determined at a 260/280 nm ratio on a Nanodrop ND-1000 (Thermo Fisher Scientific, Inc.). cDNA was synthesized by M-MLV reverse transcriptase (Promega) using random hexamer primers (Jena Bioscience GmbH). .. The expression levels of the 92 genes involved in the Wnt signaling pathway within four control genes was analyzed on a TaqMan® array human Wnt Pathway Fast 96-well plate (Invitrogen Life Technologies) using a StepOnePlus™ Real-Time PCR system (Applied Biosystems, Foster City, CA, USA).

    Random Hexamer Labeling:

    Article Title: Interferon-α Revisited: Individualized Treatment Management Eased the Selective Pressure of Tyrosine Kinase Inhibitors on BCR-ABL1 Mutations Resulting in a Molecular Response in High-Risk CML Patients
    Article Snippet: .. Total RNA was prepared from the total leukocytes of peripheral blood by Trizol (Thermofisher scientific, Waltham, MA, USA) lysis-phenol-chloroform extraction; cDNA was synthesized by M-MLV reverse transcriptase (Promega Corporation, Madison, WI, USA) using random hexamer primers (Jena Bioscience, Jena, Germany). .. GUSB (in Prague lab) or ABL1 (in Olomouc lab) were used as the control genes for the standardized quantification of BCR-ABL1 mRNA using reverse-transcriptase qPCR.

    Article Title: Application of the human estrogen receptor within a synthetic transcription factor in Trichoderma reesei
    Article Snippet: The probe was generated by PCR with the primers 5pyr4_fwd(BglII) and 5pyr4_rev-BspEI using chromosomal DNA of T. reesei Δtmus53 as template. .. Labeling of the probe was performed by using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei
    Article Snippet: Probes were generated by PCR using the primers 5Xyr1_fwd and Xyr1_1760rev-NotI (Fig. a, c) or 5pyr4_fwd(BglII) and 5pyr4_rev-BspEI (Fig. a, c) using chromosomal DNA of T. reesei Δtmus53 as template. .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Proteomic analysis of imatinib-resistant CML-T1 cells reveals calcium homeostasis as a potential therapeutic target
    Article Snippet: Wnt target gene microarray Total RNA was isolated and purified from the CML-T1 and the CML-T1/IR cells with TRIzol reagent (Thermo Fisher Scientific, Inc.) and an RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. The RNA quality and quantity were determined at a 260/280 nm ratio on a Nanodrop ND-1000 (Thermo Fisher Scientific, Inc.). cDNA was synthesized by M-MLV reverse transcriptase (Promega) using random hexamer primers (Jena Bioscience GmbH). .. The expression levels of the 92 genes involved in the Wnt signaling pathway within four control genes was analyzed on a TaqMan® array human Wnt Pathway Fast 96-well plate (Invitrogen Life Technologies) using a StepOnePlus™ Real-Time PCR system (Applied Biosystems, Foster City, CA, USA).

    Article Title: Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei
    Article Snippet: 1.5 µg of biotinylated DNA probe were used for hybridization at 65°C overnight. .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific Pierce, part of Life Technologies) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Labeling:

    Article Title: Application of the human estrogen receptor within a synthetic transcription factor in Trichoderma reesei
    Article Snippet: The probe was generated by PCR with the primers 5pyr4_fwd(BglII) and 5pyr4_rev-BspEI using chromosomal DNA of T. reesei Δtmus53 as template. .. Labeling of the probe was performed by using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei
    Article Snippet: Probes were generated by PCR using the primers 5Xyr1_fwd and Xyr1_1760rev-NotI (Fig. a, c) or 5pyr4_fwd(BglII) and 5pyr4_rev-BspEI (Fig. a, c) using chromosomal DNA of T. reesei Δtmus53 as template. .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei
    Article Snippet: 1.5 µg of biotinylated DNA probe were used for hybridization at 65°C overnight. .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific Pierce, part of Life Technologies) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

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  • 93
    Jena Bioscience random hexamer primers
    Random Hexamer Primers, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random hexamer primers/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    random hexamer primers - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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