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TaKaRa plvx ires zsgreen1
Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with <t>pLVX</t> <t>ZsGreen1,</t> pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry.
Plvx Ires Zsgreen1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plvx ires zsgreen1 - by Bioz Stars, 2020-01
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Article Title: Selection of an optimal promoter for gene transfer in normal B cells

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2017.6974

Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry.
Figure Legend Snippet: Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry.

Techniques Used: Transduction, Modification, Flow Cytometry, Cytometry

Related Articles

Amplification:

Article Title: Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins
Article Snippet: .. The NS1 cDNA sequences from A/Swine/Texas/4199-2/98 (Sw/Tx/98, H3N2), A/Nigeria/OG10/2007 (Nig/07, H5N1), A/Shanghai/2/S1078/2013 (Sh/13, H7N9), A/little yellow shouldered bat/Guatemala/153/2009 (Guat/09, HL17NL10), and A/flat-faced bat/Peru/033/2010 (Peru/10, HL18NL11) were PCR amplified from existing plasmids and ligated in frame with an N-terminal V5-tag into modified pLVX-IRES-ZsGreen1 or pLVX-IRES-Puro plasmids (Clontech, USA). .. Similar plasmids expressing GST and NS1 sequences from A/Puerto Rico/8/1934 (PR8, H1N1) or A/California/04/09 (Cal09, pdmH1N1) have been described previously ( ).

Polymerase Chain Reaction:

Article Title: Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins
Article Snippet: .. The NS1 cDNA sequences from A/Swine/Texas/4199-2/98 (Sw/Tx/98, H3N2), A/Nigeria/OG10/2007 (Nig/07, H5N1), A/Shanghai/2/S1078/2013 (Sh/13, H7N9), A/little yellow shouldered bat/Guatemala/153/2009 (Guat/09, HL17NL10), and A/flat-faced bat/Peru/033/2010 (Peru/10, HL18NL11) were PCR amplified from existing plasmids and ligated in frame with an N-terminal V5-tag into modified pLVX-IRES-ZsGreen1 or pLVX-IRES-Puro plasmids (Clontech, USA). .. Similar plasmids expressing GST and NS1 sequences from A/Puerto Rico/8/1934 (PR8, H1N1) or A/California/04/09 (Cal09, pdmH1N1) have been described previously ( ).

Modification:

Article Title: Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins
Article Snippet: .. The NS1 cDNA sequences from A/Swine/Texas/4199-2/98 (Sw/Tx/98, H3N2), A/Nigeria/OG10/2007 (Nig/07, H5N1), A/Shanghai/2/S1078/2013 (Sh/13, H7N9), A/little yellow shouldered bat/Guatemala/153/2009 (Guat/09, HL17NL10), and A/flat-faced bat/Peru/033/2010 (Peru/10, HL18NL11) were PCR amplified from existing plasmids and ligated in frame with an N-terminal V5-tag into modified pLVX-IRES-ZsGreen1 or pLVX-IRES-Puro plasmids (Clontech, USA). .. Similar plasmids expressing GST and NS1 sequences from A/Puerto Rico/8/1934 (PR8, H1N1) or A/California/04/09 (Cal09, pdmH1N1) have been described previously ( ).

Generated:

Article Title: Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins
Article Snippet: The NS1 cDNA sequences from A/Swine/Texas/4199-2/98 (Sw/Tx/98, H3N2), A/Nigeria/OG10/2007 (Nig/07, H5N1), A/Shanghai/2/S1078/2013 (Sh/13, H7N9), A/little yellow shouldered bat/Guatemala/153/2009 (Guat/09, HL17NL10), and A/flat-faced bat/Peru/033/2010 (Peru/10, HL18NL11) were PCR amplified from existing plasmids and ligated in frame with an N-terminal V5-tag into modified pLVX-IRES-ZsGreen1 or pLVX-IRES-Puro plasmids (Clontech, USA). .. Human p85α and p85β cDNA sequences from existing plasmids or the bat ( Myotis lucifugus ) p85β cDNA sequence generated using the GeneArt gene synthesis service (Thermo Fisher) were PCR amplified and ligated in frame into p3xFLAG-CMV-7.1 (Sigma-Aldrich) so as to express with N-terminal FLAG tags.

Expressing:

Article Title: Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins
Article Snippet: Paragraph title: Expression plasmids. ... The NS1 cDNA sequences from A/Swine/Texas/4199-2/98 (Sw/Tx/98, H3N2), A/Nigeria/OG10/2007 (Nig/07, H5N1), A/Shanghai/2/S1078/2013 (Sh/13, H7N9), A/little yellow shouldered bat/Guatemala/153/2009 (Guat/09, HL17NL10), and A/flat-faced bat/Peru/033/2010 (Peru/10, HL18NL11) were PCR amplified from existing plasmids and ligated in frame with an N-terminal V5-tag into modified pLVX-IRES-ZsGreen1 or pLVX-IRES-Puro plasmids (Clontech, USA).

Article Title: Selection of an optimal promoter for gene transfer in normal B cells
Article Snippet: .. To determine the levels of transgene expression in B cells three bicistronic plasmids were used that encode various GFPs under the control of different promoters, namely pGIPZ [cytomegalovirus (CMV) promoter; turbo GFP, which is an improved variant of the green fluorescent protein CopGFP], pLVTHM [elongation factor 1 alpha (EF1α) promoter; GFP) and pLVX-IRES-ZsGreen1 (CMV promoter; ZsGreen1, which is a human codon-optimized variant of ZsGreen) ( ). .. Independent of the vectors used in the present study, a very low level of transgene expression was detected in B cells; expression did not exceed 10%, as assessed with flow cytometry 7 days post-transduction ( ).

Sequencing:

Article Title: Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins
Article Snippet: The NS1 cDNA sequences from A/Swine/Texas/4199-2/98 (Sw/Tx/98, H3N2), A/Nigeria/OG10/2007 (Nig/07, H5N1), A/Shanghai/2/S1078/2013 (Sh/13, H7N9), A/little yellow shouldered bat/Guatemala/153/2009 (Guat/09, HL17NL10), and A/flat-faced bat/Peru/033/2010 (Peru/10, HL18NL11) were PCR amplified from existing plasmids and ligated in frame with an N-terminal V5-tag into modified pLVX-IRES-ZsGreen1 or pLVX-IRES-Puro plasmids (Clontech, USA). .. Human p85α and p85β cDNA sequences from existing plasmids or the bat ( Myotis lucifugus ) p85β cDNA sequence generated using the GeneArt gene synthesis service (Thermo Fisher) were PCR amplified and ligated in frame into p3xFLAG-CMV-7.1 (Sigma-Aldrich) so as to express with N-terminal FLAG tags.

Variant Assay:

Article Title: Selection of an optimal promoter for gene transfer in normal B cells
Article Snippet: .. To determine the levels of transgene expression in B cells three bicistronic plasmids were used that encode various GFPs under the control of different promoters, namely pGIPZ [cytomegalovirus (CMV) promoter; turbo GFP, which is an improved variant of the green fluorescent protein CopGFP], pLVTHM [elongation factor 1 alpha (EF1α) promoter; GFP) and pLVX-IRES-ZsGreen1 (CMV promoter; ZsGreen1, which is a human codon-optimized variant of ZsGreen) ( ). .. Independent of the vectors used in the present study, a very low level of transgene expression was detected in B cells; expression did not exceed 10%, as assessed with flow cytometry 7 days post-transduction ( ).

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    TaKaRa plvx ires zsgreen1 vector
    Plvx Ires Zsgreen1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plvx ires zsgreen1 vector/product/TaKaRa
    Average 90 stars, based on 4 article reviews
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