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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
Zimm Plot Software, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
Columnar Scatter Plot By Graphpad Prism 8.0 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
Data Analysis And Plotting Software Graphpad Prism 10, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) <t>Zimm</t> <t>plot</t> of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.
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Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) Zimm plot of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.

Journal: Pharmaceutics

Article Title: Plasmid DNA Delivery to Cancer Cells with Poly(L-lysine)-Based Copolymers Bearing Thermally Sensitive Segments: Balancing Polyplex Tightness, Transfection Efficiency, and Biocompatibility

doi: 10.3390/pharmaceutics17081012

Figure Lengend Snippet: Physicochemical characterization results of the polyplexes. ( A ) Representative particle size distribution measured at an angle of 90° for polyplexes of PNL-65 at N/P 12, prepared by method H-C. ( B ) Zimm plot of polyplexes of PNL-20 at N/P 18, prepared by method H: experimental points (open symbols); extrapolated points to zero concentration and zero scattering angle (closed symbols). ( C ) Representative AFM micrographs of the polyplex particles prepared with PNL-10 at N/P 12, using method H. ( D ) Variations of hydrodynamic diameters and ( E ) ζ potential values of polyplex particles of PNL-10, PNL-20, PNL-37, and PNL-65 with N/P. The polyplexes were prepared by the method RT. Measurements were performed at 25 °C.

Article Snippet: The SLS data were analyzed using the Zimm plot software provided by Brookhaven Instruments.

Techniques: Concentration Assay