Journal: Scientific Reports

Article Title: PLOD2 promotes proliferation, migration and invasion of colorectal cancer cells via PI3K-AKT-GSK3β signaling pathway

doi: 10.1038/s41598-026-38593-6

Figure Lengend Snippet: High expression of PLOD2 in Colorectal Cancer. ( A ) Volcano plot showed the DEGs in GSE97689 . ( B ) GO enrichment of DEGs and the list of significant GO terms of the top 10. ( C ) KEGG pathways enrichment of DEGs. ( D ) Immunohistochemical detection of PLOD2 expression in colorectal cancer tissues and adjacent normal tissues (a. Strong PLOD2 staining in colorectal cancer tissues; b. Control showing weak PLOD2 staining in colorectal cancer tissues; c. Weak PLOD2 staining in normal tissues adjacent to the tumor; d. Negative control in normal tissues adjacent to the tumor. Images captured at 50× and 400× magnification, respectively.). ( E ) Expression levels of PLOD2 in colorectal cancer and normal tissues from the CPTAC database (z-values denote the standard deviation from the median sample for a given cancer type). ( F ) Correlation of PLOD2 expression with pathological staging of colon and rectal cancer tumors (a, Colon adenocarcinoma; b, Rectal adenocarcinoma.). ( G ) Kaplan-Meier analysis of overall survival in patients with high versus low PLOD2 expression.

Article Snippet: Antibodies used in this experiment were as follows, primary antibody: PLOD2 antibody (#21214-1-AP, Wuhan Proteintech Biotechnology Co., Ltd.), GAPDH antibody (5174,Cell Signaling Technology, Inc., USA), PI3K antibody (4257, Cell Signaling Technology, Inc., USA).Phosoho-PI3K antibody (4228, Cell Signaling Technology, Inc., USA).AKT antibody (4691, Cell Signaling Technology, Inc., USA), Phosoho-AKT antibody (13038, Cell Signaling Technology, Inc., USA).

Techniques: Expressing, Immunohistochemical staining, Staining, Control, Negative Control, Standard Deviation

Journal: Scientific Reports

Article Title: PLOD2 promotes proliferation, migration and invasion of colorectal cancer cells via PI3K-AKT-GSK3β signaling pathway

doi: 10.1038/s41598-026-38593-6

Figure Lengend Snippet: PLOD2 promotes cell proliferation, migration, and invasion in CRC cells. ( A ) Western blot and qPCR analysis of PLOD2 protein and mRNA expression in HCT116-PLOD2 and Caco-2-shPLOD2 cell lines, uncropped gel and blot images with edges marked are presented in Supplementary material. ( B ) Effect of PLOD2 on the proliferative capacity of colorectal cancer cells, assessed by CCK-8 assay. ( C ) Effect of PLOD2 on colony formation and proliferative ability of colorectal cancer cells, assessed by plate colony formation assay. ( D ) Effect of PLOD2 on the horizontal migration ability of colorectal cancer cells, assessed by cell Wound healing assay. ( E ) Effect of PLOD2 on the vertical migration ability of colorectal cancer cells, assessed by Transwell migration assay. ( F ) Effect of PLOD2 on the invasive ability of colorectal cancer cells, assessed by Transwell invasion assay.

Article Snippet: Antibodies used in this experiment were as follows, primary antibody: PLOD2 antibody (#21214-1-AP, Wuhan Proteintech Biotechnology Co., Ltd.), GAPDH antibody (5174,Cell Signaling Technology, Inc., USA), PI3K antibody (4257, Cell Signaling Technology, Inc., USA).Phosoho-PI3K antibody (4228, Cell Signaling Technology, Inc., USA).AKT antibody (4691, Cell Signaling Technology, Inc., USA), Phosoho-AKT antibody (13038, Cell Signaling Technology, Inc., USA).

Techniques: Migration, Western Blot, Expressing, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay

Journal: Scientific Reports

Article Title: PLOD2 promotes proliferation, migration and invasion of colorectal cancer cells via PI3K-AKT-GSK3β signaling pathway

doi: 10.1038/s41598-026-38593-6

Figure Lengend Snippet: PLOD2 activates the PI3K-AKT-GSK3β signaling pathway in colorectal cancer cells. ( A ) KEGG enrichment analysis of PLOD2-interacting proteins and 500 expression-related genes. ( B ) Effects of PLOD2 overexpression and silencing on PI3K-AKT-GSK3β signaling pathway proteins and expression quantification, uncropped gel and blot images with edges marked are presented in Supplementary material. ( C ) Co-immunoprecipitation experiments for PLOD2 and PI3K, uncropped gel and blot images with edges marked are presented in Supplementary material.

Article Snippet: Antibodies used in this experiment were as follows, primary antibody: PLOD2 antibody (#21214-1-AP, Wuhan Proteintech Biotechnology Co., Ltd.), GAPDH antibody (5174,Cell Signaling Technology, Inc., USA), PI3K antibody (4257, Cell Signaling Technology, Inc., USA).Phosoho-PI3K antibody (4228, Cell Signaling Technology, Inc., USA).AKT antibody (4691, Cell Signaling Technology, Inc., USA), Phosoho-AKT antibody (13038, Cell Signaling Technology, Inc., USA).

Techniques: Expressing, Over Expression, Immunoprecipitation

Journal: Scientific Reports

Article Title: PLOD2 promotes proliferation, migration and invasion of colorectal cancer cells via PI3K-AKT-GSK3β signaling pathway

doi: 10.1038/s41598-026-38593-6

Figure Lengend Snippet: PLOD2 promotes CRC cell proliferation, migration, and invasion through the PI3K-AKT-GSK3β signaling pathway. ( A ) Expression of PI3K-AKT-GSK3β pathway-related proteins in colorectal cancer cells treated with the PI3K inhibitor (LY294002, 10 μM) or agonist (740Y-P, 20 μM) for 48 h, uncropped gel and blot images with edges marked are presented in Supplementary material. ( B ) CCK-8 assay assessing the proliferative capacity of colorectal cancer cells treated with PI3K inhibitors or agonists. ( C ) Colony formation assay evaluating the proliferation of colorectal cancer cells treated with PI3K inhibitors or agonists. ( D ) Wound healing assay evaluating the horizontal migration ability of colorectal cancer cells treated with PI3K inhibitors or agonists. ( E ) Transwell migration assay assessing the vertical migration ability of colorectal cancer cells treated with PI3K inhibitors or agonists. ( F ) Transwell invasion assay evaluating the invasive ability of colorectal cancer cells treated with PI3K inhibitors or agonists.

Article Snippet: Antibodies used in this experiment were as follows, primary antibody: PLOD2 antibody (#21214-1-AP, Wuhan Proteintech Biotechnology Co., Ltd.), GAPDH antibody (5174,Cell Signaling Technology, Inc., USA), PI3K antibody (4257, Cell Signaling Technology, Inc., USA).Phosoho-PI3K antibody (4228, Cell Signaling Technology, Inc., USA).AKT antibody (4691, Cell Signaling Technology, Inc., USA), Phosoho-AKT antibody (13038, Cell Signaling Technology, Inc., USA).

Techniques: Migration, Expressing, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay