platinum taq polymerase  (Thermo Fisher)


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    Name:
    Platinum Taq DNA Polymerase
    Description:
    Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step thus preventing the extention of nonspecifically annealed primers and improving product yield Using Platinum Taq DNA Polymerase The hot start property of Platinum Taq DNA Polymerase allows for convenient reaction assembly at room temperature Just as with Taq DNA Polymerase Platinum Taq DNA Polymerase has a non template dependent terminal transferase activity that adds a 3 deoxyadenosine to product ends and has a 5 →3 exonuclease activity PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic viral and plasmid templates as well as in RT PCR Note For superior PCR performance we recommend next generation enzyme Platinum II Taq Hot start DNA Polymerase Platinum II Taq Hot Start DNA Polymerase is designed for universal primer annealing and fast easy PCR with its unique combination of innovative buffer high performance engineered Taq DNA polymerase and superior hot start technology
    Catalog Number:
    10966018
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Amplification of Bisulfite-Treated DNA|Chromatin Immunoprecipitation (ChIP)|Fast PCR|Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|RNAi, Epigenetics & Non-Coding RNA Research|Routine PCR|Chromatin Biology|Methylation Analysis
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    Structured Review

    Thermo Fisher platinum taq polymerase
    Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step thus preventing the extention of nonspecifically annealed primers and improving product yield Using Platinum Taq DNA Polymerase The hot start property of Platinum Taq DNA Polymerase allows for convenient reaction assembly at room temperature Just as with Taq DNA Polymerase Platinum Taq DNA Polymerase has a non template dependent terminal transferase activity that adds a 3 deoxyadenosine to product ends and has a 5 →3 exonuclease activity PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic viral and plasmid templates as well as in RT PCR Note For superior PCR performance we recommend next generation enzyme Platinum II Taq Hot start DNA Polymerase Platinum II Taq Hot Start DNA Polymerase is designed for universal primer annealing and fast easy PCR with its unique combination of innovative buffer high performance engineered Taq DNA polymerase and superior hot start technology
    https://www.bioz.com/result/platinum taq polymerase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    platinum taq polymerase - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Melt Analysis of Mismatch Amplification Mutation Assays (Melt-MAMA): A Functional Study of a Cost-Effective SNP Genotyping Assay in Bacterial Models
    Article Snippet: .. The conventional PCR master mix comprised two forward AS primers and a common reverse primer starting at 0.2 µM (IDT, San Diego, CA), 1x PCR buffer without MgCl2 (Invitrogen, Carlsbad, CA), 2 mM MgCl2 (Invitrogen, Carlsbad, CA), 200 µM of each dNTPs (Invitrogen, Carlsbad, CA), 0.8 units of Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA), 1 µl of template at ∼1ng/µl, and molecular grade water to a final volume of 10 µl. .. Agarose-MAMAs were performed under the following conditions: PCR amplifications were conducted on a MJ Research 96 well block thermal cycler DNA engines equipped with hot bonnets.

    Article Title: Metabolic switching of human myotubes is improved by n-3 fatty acids
    Article Snippet: One microgram of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Veenendaal, The Netherlands). .. Real-time PCR was performed with platinum Taq polymerase (Invitrogen, Breda, The Netherlands) and SYBR green on an iCycler PCR machine (Bio-Rad Laboratories). .. Melt curve analysis was included to assure a single PCR product was formed.

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: Methylation Specific polymerase chain reaction (MSP-PCR) A total of 1 μg of DNA extracted from total (parental) DU145 and LNCaP cells was bisulfite modified using the EpiTect Bisulfite kit from Qiagen. .. PCR was performed using Platinum Taq Polymerase (Invitrogen) and 200 ng of either genomic or bisulfite treated DNA. ..

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    Real-time Polymerase Chain Reaction:

    Article Title: Metabolic switching of human myotubes is improved by n-3 fatty acids
    Article Snippet: One microgram of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Veenendaal, The Netherlands). .. Real-time PCR was performed with platinum Taq polymerase (Invitrogen, Breda, The Netherlands) and SYBR green on an iCycler PCR machine (Bio-Rad Laboratories). .. Melt curve analysis was included to assure a single PCR product was formed.

    SYBR Green Assay:

    Article Title: Metabolic switching of human myotubes is improved by n-3 fatty acids
    Article Snippet: One microgram of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Veenendaal, The Netherlands). .. Real-time PCR was performed with platinum Taq polymerase (Invitrogen, Breda, The Netherlands) and SYBR green on an iCycler PCR machine (Bio-Rad Laboratories). .. Melt curve analysis was included to assure a single PCR product was formed.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Novel Algorithms Reveal Streptococcal Transcriptomes and Clues about Undefined Genes
    Article Snippet: .. RT–PCR generation of amplicons was performed with the SuperScript III One-Step RT–PCR system with Platinum Taq DNA polymerase (Invitrogen) in reaction mixtures (50 μl) containing 0.2 μM of each gene-specific forward and reverse primers and 0.1 μg of DNase-treated, purified total RNA from late-log phase cultures (OD = 0.7) of strain SF370. ..

    Purification:

    Article Title: Novel Algorithms Reveal Streptococcal Transcriptomes and Clues about Undefined Genes
    Article Snippet: .. RT–PCR generation of amplicons was performed with the SuperScript III One-Step RT–PCR system with Platinum Taq DNA polymerase (Invitrogen) in reaction mixtures (50 μl) containing 0.2 μM of each gene-specific forward and reverse primers and 0.1 μg of DNase-treated, purified total RNA from late-log phase cultures (OD = 0.7) of strain SF370. ..

    Recombinant:

    Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶
    Article Snippet: We next tested each component of the nested PCR using the IAP qPCR assay in replicates of 8. .. We discovered that both recombinant Taq polymerase (Invitrogen) and the Platinum Taq polymerase (Invitrogen) tested positive for IAP sequences. ..

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    Amplification:

    Article Title: A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)
    Article Snippet: Difficulties and biases in PCR amplification have been previously reported for the AR gene (e.g., [ ]), most likely due to the high GC content in many exonic trinucleotide repeat fragments including AR . .. Many researchers have since obtained successful amplification and improved results by substituting Invitrogen Platinum Taq DNA Polymerase for the standard Invitrogen Taq DNA Polymerase (e.g., [ ]). ..

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  • 97
    Thermo Fisher platinum taq high fidelity dna polymerase
    Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid <t>DNA</t> or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding <t>Taq</t> Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P
    Platinum Taq High Fidelity Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq high fidelity dna polymerase/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    platinum taq high fidelity dna polymerase - by Bioz Stars, 2021-03
    97/100 stars
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    98
    Thermo Fisher platinum taq
    Detection of the beta-lactamase gene in commercial <t>Taq</t> polymerase. Four dilutions of <t>Amplitaq</t> DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Platinum Taq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    platinum taq - by Bioz Stars, 2021-03
    98/100 stars
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    93
    Thermo Fisher platinum taq pcr buffer
    Sequencing of <t>PCR</t> products amplified from wild-type template by <t>Taq-based</t> PNA clamp PCR. A: Chromatograms showing the sequencing of a representative PCR product amplified from wild-type template with PNA present ( bottom ) compared with a PCR product generated
    Platinum Taq Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq pcr buffer/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    platinum taq pcr buffer - by Bioz Stars, 2021-03
    93/100 stars
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    Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid DNA or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding Taq Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P

    Journal: Genes to cells : devoted to molecular & cellular mechanisms

    Article Title: Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma

    doi: 10.1111/j.1365-2443.2010.01483.x

    Figure Lengend Snippet: Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid DNA or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding Taq Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P

    Article Snippet: PCR was carried out in a 50-μL reaction mixture containing 5 μL 10× high-fidelity PCR buffer, 1 μL dNTP (10 μM each), 2 μL MgSO4 (50 μM), 4 μL primers (5 μM each), 1 μL template cDNA and 1 μL Platinum® Taq High Fidelity DNA Polymerase (5 U) (Invitrogen).

    Techniques: Recombinant, Plasmid Preparation, Transfection

    Daf1 expression in murine fibroblasts, B cells, and macrophages. A , Constitutive Daf1 mRNA level. Total RNA was extracted from the indicated cell lines and reverse transcribed. PCR was then performed using both Daf1- and β-actin-specific primers for the indicated cycle number. PCR products were run on agarose gel and visualized with ethidium bromide. L represents DNA ladder. B , A genomic fragment corresponding to the Daf1 gene 5′ flanking region (−2408/+85 from translational start site) was cloned into the promoterless vector pGL3 encoding firefly luciferase. Either this construct or the pGL3 control vector was transiently transfected into the indicated cell line along with the transfection efficiency control plasmid pRL-TK encoding the Renilla luciferase. Luciferase activities were measured 48 h posttransfection. Data (mean ± SD) have been normalized relative to the Renilla luciferase activity.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Constitutive Expression of Murine Decay-Accelerating Factor 1 Is Controlled by the Transcription Factor Sp1 1

    doi:

    Figure Lengend Snippet: Daf1 expression in murine fibroblasts, B cells, and macrophages. A , Constitutive Daf1 mRNA level. Total RNA was extracted from the indicated cell lines and reverse transcribed. PCR was then performed using both Daf1- and β-actin-specific primers for the indicated cycle number. PCR products were run on agarose gel and visualized with ethidium bromide. L represents DNA ladder. B , A genomic fragment corresponding to the Daf1 gene 5′ flanking region (−2408/+85 from translational start site) was cloned into the promoterless vector pGL3 encoding firefly luciferase. Either this construct or the pGL3 control vector was transiently transfected into the indicated cell line along with the transfection efficiency control plasmid pRL-TK encoding the Renilla luciferase. Luciferase activities were measured 48 h posttransfection. Data (mean ± SD) have been normalized relative to the Renilla luciferase activity.

    Article Snippet: Reverse transcription was then performed using SuperScript III followed by PCR amplification of the cDNA 5′ end using Platinum DNA Polymerase High Fidelity (Invitrogen Life Technologies).

    Techniques: Expressing, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Clone Assay, Plasmid Preparation, Luciferase, Construct, Transfection, Activity Assay

    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.

    Journal: PLoS ONE

    Article Title: Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria

    doi: 10.1371/journal.pone.0007010

    Figure Lengend Snippet: Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.

    Article Snippet: Taq polymerases The following DNA polymerases from commercial vendors designed for qPCR were used in the experiments reported here: Amplitaq Gold (ABI, CA; Roche lot # J02913); Platinum Taq (Invitrogen, CA; cat # 10966–026; lot 1169610); Platinum HiFi Taq (Invitrogen, CA; cat# 11304–011; lot# 1267490); HotStar Taq (Qiagen, CA; Mat # 1007837; lot # 124125007); JumpStart Taq (Sigma, MO; cat # D-6558; lot # 71K9029).

    Techniques: Labeling

    Sequencing of PCR products amplified from wild-type template by Taq-based PNA clamp PCR. A: Chromatograms showing the sequencing of a representative PCR product amplified from wild-type template with PNA present ( bottom ) compared with a PCR product generated

    Journal:

    Article Title: High-Fidelity DNA Polymerase Enhances the Sensitivity of a Peptide Nucleic Acid Clamp PCR Assay for K-ras Mutations

    doi: 10.2353/jmoldx.2008.070183

    Figure Lengend Snippet: Sequencing of PCR products amplified from wild-type template by Taq-based PNA clamp PCR. A: Chromatograms showing the sequencing of a representative PCR product amplified from wild-type template with PNA present ( bottom ) compared with a PCR product generated

    Article Snippet: The Platinum Taq -based PNA clamp PCR assay was identical to the Phusion HS assay, except for the use of 1× Platinum Taq PCR buffer (Invitrogen, Carlsbad, CA), 3 mmol/L MgCl2 , and 0.02 U/μl Platinum Taq DNA polymerase.

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification, Generated