platinum taq dna polymerase high fidelity  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Platinum Taq DNA Polymerase High Fidelity
    Description:
    Platinum Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments when high yields and robust amplification are required High fidelity is provided by a mixture of Platinum Taq DNA Polymerase and the proofreading 3 →5 exonuclease activity enzyme Pyrococcus species GB D polymerase PCR specificity is improved with the incorporation of Platinum automatic hot start technology Features of Platinum Taq DNA Polymerase High Fidelity • Fidelity greater than six times higher fidelity than Taq DNA polymerase• Amplicon size amplification of fragments up to 15 kb see figure • Convenience room temperature reaction assembly• Applications amplification of DNA from complex genomic viral and plasmid templates and RT PCRUnit definitionOne unit of Platinum Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C
    Catalog Number:
    11304011
    Price:
    None
    Applications:
    Amplification of Bisulfite-Treated DNA|Chromatin Immunoprecipitation (ChIP)|High Fidelity PCR|Hot Start PCR|Kits & Reagents for Sanger Sequencing|Long Fragment PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|RNAi, Epigenetics & Non-Coding RNA Research|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Chromatin Biology|Methylation Analysis|Sequencing
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher platinum taq dna polymerase high fidelity
    RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum <t>Taq</t> <t>DNA</t> Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.
    Platinum Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments when high yields and robust amplification are required High fidelity is provided by a mixture of Platinum Taq DNA Polymerase and the proofreading 3 →5 exonuclease activity enzyme Pyrococcus species GB D polymerase PCR specificity is improved with the incorporation of Platinum automatic hot start technology Features of Platinum Taq DNA Polymerase High Fidelity • Fidelity greater than six times higher fidelity than Taq DNA polymerase• Amplicon size amplification of fragments up to 15 kb see figure • Convenience room temperature reaction assembly• Applications amplification of DNA from complex genomic viral and plasmid templates and RT PCRUnit definitionOne unit of Platinum Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C
    https://www.bioz.com/result/platinum taq dna polymerase high fidelity/product/Thermo Fisher
    Average 99 stars, based on 894 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase high fidelity - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs"

    Article Title: Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs

    Journal: Oncology Letters

    doi: 10.3892/ol.2010.185

    RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum Taq DNA Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.
    Figure Legend Snippet: RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum Taq DNA Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Touchdown PCR

    2) Product Images from "Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs"

    Article Title: Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs

    Journal: Oncology Letters

    doi: 10.3892/ol.2010.185

    RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum Taq DNA Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.
    Figure Legend Snippet: RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum Taq DNA Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Touchdown PCR

    3) Product Images from "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity"

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    Journal: BioMed Research International

    doi: 10.1155/2018/5657085

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Figure Legend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Techniques Used: DNA Sequencing, Sequencing, Activity Assay

    4) Product Images from "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity"

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    Journal: BioMed Research International

    doi: 10.1155/2018/5657085

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Figure Legend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Techniques Used: DNA Sequencing, Sequencing, Activity Assay

    5) Product Images from "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity"

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    Journal: BioMed Research International

    doi: 10.1155/2018/5657085

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Figure Legend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Techniques Used: DNA Sequencing, Sequencing, Activity Assay

    6) Product Images from "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity"

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    Journal: BioMed Research International

    doi: 10.1155/2018/5657085

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Figure Legend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Techniques Used: DNA Sequencing, Sequencing, Activity Assay

    7) Product Images from "Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system"

    Article Title: Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1038/mtm.2014.50

    Development of a TA cloning- and RE digestion-based method to examine genetic rearrangements of TALE repeat arrays. ( a ) Work flow chart. A fusion expression cassette bearing both left and right TALEN arms (TALEN L-R) is amplified with forward and reverse PCR primers (FP and RP) designed to amplify the full-length of tandem repeat arrays. The PCR products are inserted into a TA cloning vector. EcoRI digestion of individual clones followed by agarose gel electrophoresis is used to detect the size change of the TALE repeat arrays. The detection of a 1.76 kb DNA fragment indicates that the number of the tandem repeat units remains correct in the TALEN expression cassette. To further classify the genetic rearrangement events, the clones with no obvious size changes are subjected to DNA sequencing. ( b ) The use of an enzyme mixture allows PCR amplification of single band. Left: Platinum Taq DNA Polymerase High Fidelity. Right: Platinum Taq DNA Polymerase High Fidelity mixed with Elongase (1:2). ( c ) TA cloning and EcoRI digestion detect no change in the size of the TALE repeat arrays in plasmid vector samples. The band at 3 kb after EcoRI digestion is the linearized cloning vector, while the DNA band at 1.76 kb is the DNA insert bearing a TALE repeat array. “–“ and “+”: Without and with EcoRI. One kb DNA ladder was used as a molecular weight standard. ( d ) DNA sequencing analysis confirms no change in DNA recognition specificity by RVDs in left (top panel) and right (bottom panel) TALEN arms in the above plasmid samples. Amino acid sequence alignment was performed after translating DNA sequences into amino acids. The pair letters in the sequence represent a RVD (HD, NI, NG, and NN) for each TALE repeat unit in a TALEN arm.
    Figure Legend Snippet: Development of a TA cloning- and RE digestion-based method to examine genetic rearrangements of TALE repeat arrays. ( a ) Work flow chart. A fusion expression cassette bearing both left and right TALEN arms (TALEN L-R) is amplified with forward and reverse PCR primers (FP and RP) designed to amplify the full-length of tandem repeat arrays. The PCR products are inserted into a TA cloning vector. EcoRI digestion of individual clones followed by agarose gel electrophoresis is used to detect the size change of the TALE repeat arrays. The detection of a 1.76 kb DNA fragment indicates that the number of the tandem repeat units remains correct in the TALEN expression cassette. To further classify the genetic rearrangement events, the clones with no obvious size changes are subjected to DNA sequencing. ( b ) The use of an enzyme mixture allows PCR amplification of single band. Left: Platinum Taq DNA Polymerase High Fidelity. Right: Platinum Taq DNA Polymerase High Fidelity mixed with Elongase (1:2). ( c ) TA cloning and EcoRI digestion detect no change in the size of the TALE repeat arrays in plasmid vector samples. The band at 3 kb after EcoRI digestion is the linearized cloning vector, while the DNA band at 1.76 kb is the DNA insert bearing a TALE repeat array. “–“ and “+”: Without and with EcoRI. One kb DNA ladder was used as a molecular weight standard. ( d ) DNA sequencing analysis confirms no change in DNA recognition specificity by RVDs in left (top panel) and right (bottom panel) TALEN arms in the above plasmid samples. Amino acid sequence alignment was performed after translating DNA sequences into amino acids. The pair letters in the sequence represent a RVD (HD, NI, NG, and NN) for each TALE repeat unit in a TALEN arm.

    Techniques Used: TA Cloning, Flow Cytometry, Expressing, Amplification, Polymerase Chain Reaction, Plasmid Preparation, Clone Assay, Agarose Gel Electrophoresis, DNA Sequencing, Molecular Weight, Sequencing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Constitutive Expression of Murine Decay-Accelerating Factor 1 Is Controlled by the Transcription Factor Sp1 1
    Article Snippet: .. Reverse transcription was then performed using SuperScript III followed by PCR amplification of the cDNA 5′ end using Platinum DNA Polymerase High Fidelity (Invitrogen Life Technologies). .. The following primers were used for amplification: Gene Racer forward primer and Daf1 race reverse 5′-CCGCG TACAGTTGGGGACAGCAGCAAC-3′.

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. Platinum™ Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) was used to amplify DNA fragments by PCR in GeneAmp PCR system 2700 thermal cycler (Applied Biosystems, Foster City, CA, USA). .. PCR products were purified with QiAquick PCR purification kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations.

    Article Title: Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs
    Article Snippet: .. For the detection of the wild-type normal JAZF1 and SUZ12 transcripts, PCR was performed with Platinum Taq DNA Polymerase High Fidelity (Invitrogen). .. The 50-μl reaction volume contained 1X High Fidelity PCR buffer, 0.2 mM of each dNTP, 2 mM MgSO4 , 0.2 μM of each of the forward and reverse primers, 1 unit Platinum Taq DNA Polymerase High Fidelity and 1 μl of the cDNA.

    Article Title: Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs
    Article Snippet: .. For the detection of the JAZF1/SUZ12 fusion transcript, PCR was performed with Platinum Taq DNA Polymerase High Fidelity (Invitrogen). .. The 50-μl reaction volume contained 1X High Fidelity PCR buffer, 0.2 mM of each dNTP, 2 mM MgSO4 , 0.2 μM of each of the forward and reverse primers, 1 unit Platinum Taq DNA Polymerase High Fidelity and 1 μl of the cDNA.

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragments of LraI operon amplified using Platinum™ Taq DNA Polymerase High Fidelity were cloned into pAZIL vector predigested with Sma I. .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Article Title: Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein
    Article Snippet: .. PCR reaction was carried out by using a high fidelity DNA polymerase (Platinum® Taq DNA Polymerase High Fidelity, Invitrogen). .. Oligonucleotides used for the reaction were: VpuBF/PacI (5'- T T A AT T AAGCTTCTCTATCAAAGC-3') VpuBF/Eco47III (5'-AG C GCTAAAGAAAAATTGTGGGTC-3') Modifications in the oligonucleotides allowed obtaining a PCR product harboring both PacI and Eco47III restriction sites at the 5' and 3' ends of the vpu coding sequence respectively.

    Article Title: Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma
    Article Snippet: .. PCR was carried out in a 50-μL reaction mixture containing 5 μL 10× high-fidelity PCR buffer, 1 μL dNTP (10 μM each), 2 μL MgSO4 (50 μM), 4 μL primers (5 μM each), 1 μL template cDNA and 1 μL Platinum® Taq High Fidelity DNA Polymerase (5 U) (Invitrogen). .. Amplification was carried out at 94 °C for 4 min, 38 cycles of 94 °C for 30 s, 55 °C for 1 min, 72 °C for 30 s, and a final extension at 72 °C for 7 min. PCR products were electrophoresed on a 1.5% SuperAcryl agarose gel containing 25 ng/mL ethidium bromide, gel purified, sequenced, and cloned into pcDNA 3.1.V5-His-TOPO® TA expression vector (Invitrogen).

    Real-time Polymerase Chain Reaction:

    Article Title: Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria
    Article Snippet: .. Taq polymerases The following DNA polymerases from commercial vendors designed for qPCR were used in the experiments reported here: Amplitaq Gold (ABI, CA; Roche lot # J02913); Platinum Taq (Invitrogen, CA; cat # 10966–026; lot 1169610); Platinum HiFi Taq (Invitrogen, CA; cat# 11304–011; lot# 1267490); HotStar Taq (Qiagen, CA; Mat # 1007837; lot # 124125007); JumpStart Taq (Sigma, MO; cat # D-6558; lot # 71K9029). .. Two polymerases not designed specifically for qPCR (no anti-Taq antibody) were also tested: Amplitaq DNA polymerase (ABI, CA; Roche lot # C00622); Qiagen Taq DNA polymerase (Qiagen, CA; Cat # 201205, lot # 127132149.

    Amplification:

    Article Title: Constitutive Expression of Murine Decay-Accelerating Factor 1 Is Controlled by the Transcription Factor Sp1 1
    Article Snippet: .. Reverse transcription was then performed using SuperScript III followed by PCR amplification of the cDNA 5′ end using Platinum DNA Polymerase High Fidelity (Invitrogen Life Technologies). .. The following primers were used for amplification: Gene Racer forward primer and Daf1 race reverse 5′-CCGCG TACAGTTGGGGACAGCAGCAAC-3′.

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragments of LraI operon amplified using Platinum™ Taq DNA Polymerase High Fidelity were cloned into pAZIL vector predigested with Sma I. .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Plasmid Preparation:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragments of LraI operon amplified using Platinum™ Taq DNA Polymerase High Fidelity were cloned into pAZIL vector predigested with Sma I. .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Clone Assay:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragments of LraI operon amplified using Platinum™ Taq DNA Polymerase High Fidelity were cloned into pAZIL vector predigested with Sma I. .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher platinum taq dna polymerase high fidelity
    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by <t>DNA</t> sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the <t>Taq</t> DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Platinum Taq Dna Polymerase High Fidelity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq dna polymerase high fidelity/product/Thermo Fisher
    Average 99 stars, based on 894 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase high fidelity - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Journal: BioMed Research International

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    doi: 10.1155/2018/5657085

    Figure Lengend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Article Snippet: PCR fragments of LraI operon amplified using Platinum™ Taq DNA Polymerase High Fidelity were cloned into pAZIL vector predigested with Sma I.

    Techniques: DNA Sequencing, Sequencing, Activity Assay

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Journal: BioMed Research International

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    doi: 10.1155/2018/5657085

    Figure Lengend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Article Snippet: Platinum™ Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) was used to amplify DNA fragments by PCR in GeneAmp PCR system 2700 thermal cycler (Applied Biosystems, Foster City, CA, USA).

    Techniques: DNA Sequencing, Sequencing, Activity Assay