platinum taq dna polymerase high fidelity  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    New England Biolabs platinum taq dna polymerase high fidelity
    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by <t>DNA</t> sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the <t>Taq</t> DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Platinum Taq Dna Polymerase High Fidelity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq dna polymerase high fidelity/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase high fidelity - by Bioz Stars, 2020-04
    94/100 stars

    Images

    1) Product Images from "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity"

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    Journal: BioMed Research International

    doi: 10.1155/2018/5657085

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Figure Legend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Techniques Used: DNA Sequencing, Sequencing, Activity Assay

    Related Articles

    Clone Assay:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). ..

    Recombinant:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: Paragraph title: 2.4. Recombinant Lra I Restriction Endonuclease Overexpression in E. coli and Purification ... PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Construct:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). ..

    Purification:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). ..

    Polymerase Chain Reaction:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). ..

    Expressing:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). .. Expression of recombinant protein was carried out at 23°C by induction with 0.1 mM isopropyl β -D-1-thiogalactopyranoside (IPTG).

    Sequencing:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). .. Confirmation of fragment presence in adequate orientation was obtained by restriction enzyme analysis (with Sac I and Hin dIII digestion) and sequencing.

    Transformation Assay:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). ..

    Over Expression:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: Paragraph title: 2.4. Recombinant Lra I Restriction Endonuclease Overexpression in E. coli and Purification ... PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Plasmid Preparation:

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
    Article Snippet: .. PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs platinum taq dna polymerase high fidelity
    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by <t>DNA</t> sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the <t>Taq</t> DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Platinum Taq Dna Polymerase High Fidelity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq dna polymerase high fidelity/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase high fidelity - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Journal: BioMed Research International

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    doi: 10.1155/2018/5657085

    Figure Lengend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Article Snippet: PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Techniques: DNA Sequencing, Sequencing, Activity Assay

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Journal: BioMed Research International

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    doi: 10.1155/2018/5657085

    Figure Lengend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Article Snippet: Recombinant Lra I Restriction Endonuclease Overexpression in E. coli and Purification PCR fragment consisted of lraIR gene (from ATG to stop codon) obtained by LraI-Fw/LraI-Rev primers and Platinum™ Taq DNA Polymerase High Fidelity was purified, digested with Hin dIII and cloned into pMAL-c5X vector digested with Xmn I and Hin dIII restriction enzymes and transformed into ER2523 competent cells (New England Biolabs, Ltd. UK) previously transformed with pAZIL-LraRM construct ( ).

    Techniques: DNA Sequencing, Sequencing, Activity Assay