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shsmarcc2 oligonucleotide sequences  (Addgene inc)


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    Structured Review

    Addgene inc shsmarcc2 oligonucleotide sequences
    Shsmarcc2 Oligonucleotide Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+sequence/pLKO%2E1-blast+(Plasmid+%2326655)/pm42844465-697-2-15
    Average 94 stars, based on 144 article reviews
    shsmarcc2 oligonucleotide sequences - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A commensally regulated immune rheostat fine-tunes skin barrier fitness
    Article Snippet: .. In brief, Cas9 guides selected by VBC.core (Table S2) were cloned into the plasmid sequence (Addgene; plasmid no. 57823 ( )) containing a ubiquitous phosphoglycerate kinase promoter (Pgk)–driven Tag-RFP to identify transduced cells. ..

    Plasmid Preparation:

    Article Title: A commensally regulated immune rheostat fine-tunes skin barrier fitness
    Article Snippet: .. In brief, Cas9 guides selected by VBC.core (Table S2) were cloned into the plasmid sequence (Addgene; plasmid no. 57823 ( )) containing a ubiquitous phosphoglycerate kinase promoter (Pgk)–driven Tag-RFP to identify transduced cells. ..

    Article Title: A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae
    Article Snippet: CelTag plasmid is available via Addgene (Plasmid #66562). .. Full plasmid sequence is available in File S1 and from Addgene. ..

    Article Title: Alanine catabolism as a targetable vulnerability for MYC-driven liver cancer.
    Article Snippet: The pT3-MYC-IRES-CRE vector was synthesized by GeneScript. .. The plasmid sequence was identical to that of the pT3-EF1a-MYCIRES-LUC plasmid (Addgene, 129775), except the two LoxP sites were deleted, and the Luciferase coding sequence was replaced with the Cre coding sequence. ..

    Article Title: Using a GPT-5-driven autonomous lab to optimize the cost and titer of cell-free protein synthesis
    Article Snippet: .. The plasmid sequence was based on pJL1-sfGFP (Addgene plasmid #69496; http://n2t.net/addgene:69496 ) used in state of the art by Olsen et al . (2025). ..

    Article Title: Alanine Catabolism as a Targetable Vulnerability for MYC-Driven Liver Cancer
    Article Snippet: The pT3-MYC-IRES-CRE vector was synthesized by GeneScript. .. The plasmid sequence was identical to that of the pT3-EF1a-MYC-IRES-LUC plasmid (Addgene, 129775), except the two LoxP sites were deleted, and the Luciferase coding sequence was replaced with the Cre coding sequence. .. Hydrodynamic tail vein injection was done on wildtype male C57BL/6 (JAX, 000664) or GPT2 FLOX ( ) mice of 6-8 weeks of age as done in reference ( ).

    Article Title: WNK2 variants associated with familial osteoarthritis alter the chondrocyte response to hyperosmotic stress
    Article Snippet: Generation of WNK2 mutant constructs WT and mutant WNK2 constructs were synthesized using GenScript’s CloneEZ service. .. The WT WNK2 construct was derived from a plasmid sequence deposited in Addgene (Catalog #24569) and subsequently modified by GenScript to generate the OA-associated variants, WNK2H758N, WNK2L1005F, and WNK2R2054Q. ..

    Article Title: DNA Break-Induced Epigenetic Alterations Promote Plaque Formation and Behavioral Deficits in an Alzheimer’s Disease Mouse Model
    Article Snippet: A positive control plasmid p201B/Cas9 (#59177, Addgene, Watertown, MA, USA ( )) was used to confirm the proper activation of immunoprecipitated I- Ppo I endonuclease as described above. .. The p201B/Cas9 plasmid along with the hu-APP PCR product underwent a series of three digestion reactions overnight in a 37°C incubator (Genie Temp-Shaker 300, LabRepCo, Horsham, PA, USA): a reaction with no restriction enzyme, a reaction with immunoprecipitated I- Ppo I endonuclease, and a reaction with immunoprecipitated I- Ppo I endonuclease with an additional restriction enzyme confirmed to have a recognition site for that plasmid sequence (#59177, Addgene, Watertown, MA, USA ( ); AgeI-HF [p201B/Cas9] and BbSI-HF [hu-APP], New England Biolabs, Ipswich, MA, USA). .. All digestion reactions were mixed with DNA loading buffer and separated on a 1% agarose gel for visualization (ChemiDoc, Bio-Rad, Hercules, CA, USA).

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Plasmid sequence is available at Addgene (Plasmid #239294). ..

    Sequencing:

    Article Title: A commensally regulated immune rheostat fine-tunes skin barrier fitness
    Article Snippet: .. In brief, Cas9 guides selected by VBC.core (Table S2) were cloned into the plasmid sequence (Addgene; plasmid no. 57823 ( )) containing a ubiquitous phosphoglycerate kinase promoter (Pgk)–driven Tag-RFP to identify transduced cells. ..

    Article Title: A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae
    Article Snippet: CelTag plasmid is available via Addgene (Plasmid #66562). .. Full plasmid sequence is available in File S1 and from Addgene. ..

    Article Title: Alanine catabolism as a targetable vulnerability for MYC-driven liver cancer.
    Article Snippet: The pT3-MYC-IRES-CRE vector was synthesized by GeneScript. .. The plasmid sequence was identical to that of the pT3-EF1a-MYCIRES-LUC plasmid (Addgene, 129775), except the two LoxP sites were deleted, and the Luciferase coding sequence was replaced with the Cre coding sequence. ..

    Article Title: Using a GPT-5-driven autonomous lab to optimize the cost and titer of cell-free protein synthesis
    Article Snippet: .. The plasmid sequence was based on pJL1-sfGFP (Addgene plasmid #69496; http://n2t.net/addgene:69496 ) used in state of the art by Olsen et al . (2025). ..

    Article Title: Alanine Catabolism as a Targetable Vulnerability for MYC-Driven Liver Cancer
    Article Snippet: The pT3-MYC-IRES-CRE vector was synthesized by GeneScript. .. The plasmid sequence was identical to that of the pT3-EF1a-MYC-IRES-LUC plasmid (Addgene, 129775), except the two LoxP sites were deleted, and the Luciferase coding sequence was replaced with the Cre coding sequence. .. Hydrodynamic tail vein injection was done on wildtype male C57BL/6 (JAX, 000664) or GPT2 FLOX ( ) mice of 6-8 weeks of age as done in reference ( ).

    Article Title: WNK2 variants associated with familial osteoarthritis alter the chondrocyte response to hyperosmotic stress
    Article Snippet: Generation of WNK2 mutant constructs WT and mutant WNK2 constructs were synthesized using GenScript’s CloneEZ service. .. The WT WNK2 construct was derived from a plasmid sequence deposited in Addgene (Catalog #24569) and subsequently modified by GenScript to generate the OA-associated variants, WNK2H758N, WNK2L1005F, and WNK2R2054Q. ..

    Article Title: DNA Break-Induced Epigenetic Alterations Promote Plaque Formation and Behavioral Deficits in an Alzheimer’s Disease Mouse Model
    Article Snippet: A positive control plasmid p201B/Cas9 (#59177, Addgene, Watertown, MA, USA ( )) was used to confirm the proper activation of immunoprecipitated I- Ppo I endonuclease as described above. .. The p201B/Cas9 plasmid along with the hu-APP PCR product underwent a series of three digestion reactions overnight in a 37°C incubator (Genie Temp-Shaker 300, LabRepCo, Horsham, PA, USA): a reaction with no restriction enzyme, a reaction with immunoprecipitated I- Ppo I endonuclease, and a reaction with immunoprecipitated I- Ppo I endonuclease with an additional restriction enzyme confirmed to have a recognition site for that plasmid sequence (#59177, Addgene, Watertown, MA, USA ( ); AgeI-HF [p201B/Cas9] and BbSI-HF [hu-APP], New England Biolabs, Ipswich, MA, USA). .. All digestion reactions were mixed with DNA loading buffer and separated on a 1% agarose gel for visualization (ChemiDoc, Bio-Rad, Hercules, CA, USA).

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Plasmid sequence is available at Addgene (Plasmid #239294). ..

    Luciferase:

    Article Title: Alanine catabolism as a targetable vulnerability for MYC-driven liver cancer.
    Article Snippet: The pT3-MYC-IRES-CRE vector was synthesized by GeneScript. .. The plasmid sequence was identical to that of the pT3-EF1a-MYCIRES-LUC plasmid (Addgene, 129775), except the two LoxP sites were deleted, and the Luciferase coding sequence was replaced with the Cre coding sequence. ..

    Article Title: Alanine Catabolism as a Targetable Vulnerability for MYC-Driven Liver Cancer
    Article Snippet: The pT3-MYC-IRES-CRE vector was synthesized by GeneScript. .. The plasmid sequence was identical to that of the pT3-EF1a-MYC-IRES-LUC plasmid (Addgene, 129775), except the two LoxP sites were deleted, and the Luciferase coding sequence was replaced with the Cre coding sequence. .. Hydrodynamic tail vein injection was done on wildtype male C57BL/6 (JAX, 000664) or GPT2 FLOX ( ) mice of 6-8 weeks of age as done in reference ( ).

    Construct:

    Article Title: WNK2 variants associated with familial osteoarthritis alter the chondrocyte response to hyperosmotic stress
    Article Snippet: Generation of WNK2 mutant constructs WT and mutant WNK2 constructs were synthesized using GenScript’s CloneEZ service. .. The WT WNK2 construct was derived from a plasmid sequence deposited in Addgene (Catalog #24569) and subsequently modified by GenScript to generate the OA-associated variants, WNK2H758N, WNK2L1005F, and WNK2R2054Q. ..

    Derivative Assay:

    Article Title: WNK2 variants associated with familial osteoarthritis alter the chondrocyte response to hyperosmotic stress
    Article Snippet: Generation of WNK2 mutant constructs WT and mutant WNK2 constructs were synthesized using GenScript’s CloneEZ service. .. The WT WNK2 construct was derived from a plasmid sequence deposited in Addgene (Catalog #24569) and subsequently modified by GenScript to generate the OA-associated variants, WNK2H758N, WNK2L1005F, and WNK2R2054Q. ..

    Modification:

    Article Title: WNK2 variants associated with familial osteoarthritis alter the chondrocyte response to hyperosmotic stress
    Article Snippet: Generation of WNK2 mutant constructs WT and mutant WNK2 constructs were synthesized using GenScript’s CloneEZ service. .. The WT WNK2 construct was derived from a plasmid sequence deposited in Addgene (Catalog #24569) and subsequently modified by GenScript to generate the OA-associated variants, WNK2H758N, WNK2L1005F, and WNK2R2054Q. ..

    Polymerase Chain Reaction:

    Article Title: DNA Break-Induced Epigenetic Alterations Promote Plaque Formation and Behavioral Deficits in an Alzheimer’s Disease Mouse Model
    Article Snippet: A positive control plasmid p201B/Cas9 (#59177, Addgene, Watertown, MA, USA ( )) was used to confirm the proper activation of immunoprecipitated I- Ppo I endonuclease as described above. .. The p201B/Cas9 plasmid along with the hu-APP PCR product underwent a series of three digestion reactions overnight in a 37°C incubator (Genie Temp-Shaker 300, LabRepCo, Horsham, PA, USA): a reaction with no restriction enzyme, a reaction with immunoprecipitated I- Ppo I endonuclease, and a reaction with immunoprecipitated I- Ppo I endonuclease with an additional restriction enzyme confirmed to have a recognition site for that plasmid sequence (#59177, Addgene, Watertown, MA, USA ( ); AgeI-HF [p201B/Cas9] and BbSI-HF [hu-APP], New England Biolabs, Ipswich, MA, USA). .. All digestion reactions were mixed with DNA loading buffer and separated on a 1% agarose gel for visualization (ChemiDoc, Bio-Rad, Hercules, CA, USA).

    Immunoprecipitation:

    Article Title: DNA Break-Induced Epigenetic Alterations Promote Plaque Formation and Behavioral Deficits in an Alzheimer’s Disease Mouse Model
    Article Snippet: A positive control plasmid p201B/Cas9 (#59177, Addgene, Watertown, MA, USA ( )) was used to confirm the proper activation of immunoprecipitated I- Ppo I endonuclease as described above. .. The p201B/Cas9 plasmid along with the hu-APP PCR product underwent a series of three digestion reactions overnight in a 37°C incubator (Genie Temp-Shaker 300, LabRepCo, Horsham, PA, USA): a reaction with no restriction enzyme, a reaction with immunoprecipitated I- Ppo I endonuclease, and a reaction with immunoprecipitated I- Ppo I endonuclease with an additional restriction enzyme confirmed to have a recognition site for that plasmid sequence (#59177, Addgene, Watertown, MA, USA ( ); AgeI-HF [p201B/Cas9] and BbSI-HF [hu-APP], New England Biolabs, Ipswich, MA, USA). .. All digestion reactions were mixed with DNA loading buffer and separated on a 1% agarose gel for visualization (ChemiDoc, Bio-Rad, Hercules, CA, USA).



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