plasmid plus midi kit  (Qiagen)

 
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    Name:
    QIAGEN Plasmid Plus Midi Kit
    Description:
    For the fastest most convenient purification of up to 10 mg transfection grade plasmid DNA Kit contents Qiagen Plasmid Plus Midi Kit 25 preps 25 to 50mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Advanced Silica Technology Manual Processing 20 min Time Run Up to 1mg Yield Ideal Transfection of most Cell Lines Cloning Enzymatic Restriction For the Fastest Most Convenient Purification of up to 250μg Transfection grade Plasmid DNA Includes 25 Qiagen Plasmid Plus Midi Columns Extender Tubes Reagents Buffers 25 QIAfilter Midi Cartridges Benefits Rapid purification of up to 24 samples Fast vacuum based parallel processing Low elution volumes for highly concentrated DNA Very low endotoxin levels 1 EU μg due to dedicated wash buffer
    Catalog Number:
    12943
    Price:
    342
    Category:
    QIAGEN Plasmid Plus Kits
    Buy from Supplier


    Structured Review

    Qiagen plasmid plus midi kit
    QIAGEN Plasmid Plus Midi Kit
    For the fastest most convenient purification of up to 10 mg transfection grade plasmid DNA Kit contents Qiagen Plasmid Plus Midi Kit 25 preps 25 to 50mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Advanced Silica Technology Manual Processing 20 min Time Run Up to 1mg Yield Ideal Transfection of most Cell Lines Cloning Enzymatic Restriction For the Fastest Most Convenient Purification of up to 250μg Transfection grade Plasmid DNA Includes 25 Qiagen Plasmid Plus Midi Columns Extender Tubes Reagents Buffers 25 QIAfilter Midi Cartridges Benefits Rapid purification of up to 24 samples Fast vacuum based parallel processing Low elution volumes for highly concentrated DNA Very low endotoxin levels 1 EU μg due to dedicated wash buffer
    https://www.bioz.com/result/plasmid plus midi kit/product/Qiagen
    Average 99 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    plasmid plus midi kit - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Conjugation Assay:

    Article Title: Prevalence of Salmonella Isolates from Chicken and Pig Slaughterhouses and Emergence of Ciprofloxacin and Cefotaxime Co-Resistant S. enterica Serovar Indiana in Henan, China
    Article Snippet: .. Plasmid replicon typing and conjugation experiment of S . enterica serovar Indiana isolates Plasmids were isolated using Plasmid Plus Midi kit (Qiagen, Germany). .. Replicon typing of plasmids was performed using a PCR-based method as previously described [ ].

    Transfection:

    Article Title: Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii
    Article Snippet: .. Plasmid vectors for microinjections were prepared using NucleoSpin® Plasmid Transfection-grade (Macherey-Nagel) or QIAGEN Plasmid Plus Midi Kit (QIAGEN GmbH, 40,724 Hilden, Germany). .. Fly strain and husbandry All fly experiments were performed in our well-equipped safety level one (S1) laboratory, which is certified for generating and using genetically modified insects.

    Amplification:

    Article Title: Human Serum Albumin-Based Nanoparticle-Mediated In Vitro Gene Delivery
    Article Snippet: .. Bacterial colonies were amplified in ampicillin-containing medium, and the plasmid cDNA was purified using the QIAGEN Plasmid Plus Midi Kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s high yield protocol. .. The plasmid content was determined with a Nanodrop 2000™ spectrophotometer (Thermo Scientific, Wilmington, USA), and the plasmid cDNA stocks were stored at −20°C.

    Clone Assay:

    Article Title: Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme
    Article Snippet: .. The cloned plasmid was isolated from E. coli using Mini or Midi column plasmid purification kits (Qiagen, Inc., Santa Clarita, Calif.). .. Restriction enzyme digestion (visualized on a 0.8% ethidium bromide agarose gel) and DNA sequence analysis of the plasmid indicated that pepO was in frame and in a proper orientation to allow for the production of the fusion protein.

    Ligation:

    Article Title: Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity
    Article Snippet: .. Following ligation, plasmids were transformed into chemically competent Top10 cells, and plasmids were extracted and purified using the QIAamp DNA Mini or Plasmid Plus Midi Kit (all from Qiagen). .. Cell culture Before use, all cell lines were authenticated and tested negative for mycoplasma contamination via a commercial service (Multiplexion, Heidelberg).

    Isolation:

    Article Title: Prevalence of Salmonella Isolates from Chicken and Pig Slaughterhouses and Emergence of Ciprofloxacin and Cefotaxime Co-Resistant S. enterica Serovar Indiana in Henan, China
    Article Snippet: .. Plasmid replicon typing and conjugation experiment of S . enterica serovar Indiana isolates Plasmids were isolated using Plasmid Plus Midi kit (Qiagen, Germany). .. Replicon typing of plasmids was performed using a PCR-based method as previously described [ ].

    Article Title: Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme
    Article Snippet: .. The cloned plasmid was isolated from E. coli using Mini or Midi column plasmid purification kits (Qiagen, Inc., Santa Clarita, Calif.). .. Restriction enzyme digestion (visualized on a 0.8% ethidium bromide agarose gel) and DNA sequence analysis of the plasmid indicated that pepO was in frame and in a proper orientation to allow for the production of the fusion protein.

    Article Title: Random and targeted transgene insertion in C. elegans using a modified Mosl transposon
    Article Snippet: .. We isolated DNA with Spin Miniprep (cat. no. 27106) and Plasmid Plus Midiprep (cat. no. 12943) kits from Qiagen and with a PureLink HQ Mini Plasmid kit from Invitrogen (cat. no. K2100-01). .. The higher quality DNA kits (Qiagen Midi and Invitrogen Mini kits) resulted in a four-fold increase in F1-rescued animals (20 vs 5 rescued animals per injection) and a 50% (Qiagen Midi) to 100% (Invitrogen mini) increase in mosSCI insertion frequency ( ).

    Purification:

    Article Title: Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity
    Article Snippet: .. Following ligation, plasmids were transformed into chemically competent Top10 cells, and plasmids were extracted and purified using the QIAamp DNA Mini or Plasmid Plus Midi Kit (all from Qiagen). .. Cell culture Before use, all cell lines were authenticated and tested negative for mycoplasma contamination via a commercial service (Multiplexion, Heidelberg).

    Article Title: Human Serum Albumin-Based Nanoparticle-Mediated In Vitro Gene Delivery
    Article Snippet: .. Bacterial colonies were amplified in ampicillin-containing medium, and the plasmid cDNA was purified using the QIAGEN Plasmid Plus Midi Kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s high yield protocol. .. The plasmid content was determined with a Nanodrop 2000™ spectrophotometer (Thermo Scientific, Wilmington, USA), and the plasmid cDNA stocks were stored at −20°C.

    Article Title: Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme
    Article Snippet: .. The cloned plasmid was isolated from E. coli using Mini or Midi column plasmid purification kits (Qiagen, Inc., Santa Clarita, Calif.). .. Restriction enzyme digestion (visualized on a 0.8% ethidium bromide agarose gel) and DNA sequence analysis of the plasmid indicated that pepO was in frame and in a proper orientation to allow for the production of the fusion protein.

    Article Title: Immunogenicity and Protection Efficacy of a Naked Self-Replicating mRNA-Based Zika Virus Vaccine
    Article Snippet: .. After 16 h, the bacteria were lysed and the plasmids were purified using the Plasmid Plus Midi kit (Qiagen, Germany). .. Subsequently, the plasmids were linearized with I-SceI endonuclease (NEB, Massachusetts, USA) and sr-mRNA were synthetized by IVT with the MEGAscript T7 Transcription kit (Life Technologies, Massachusetts, USA).

    Transformation Assay:

    Article Title: Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity
    Article Snippet: .. Following ligation, plasmids were transformed into chemically competent Top10 cells, and plasmids were extracted and purified using the QIAamp DNA Mini or Plasmid Plus Midi Kit (all from Qiagen). .. Cell culture Before use, all cell lines were authenticated and tested negative for mycoplasma contamination via a commercial service (Multiplexion, Heidelberg).

    Plasmid Preparation:

    Article Title: Prevalence of Salmonella Isolates from Chicken and Pig Slaughterhouses and Emergence of Ciprofloxacin and Cefotaxime Co-Resistant S. enterica Serovar Indiana in Henan, China
    Article Snippet: .. Plasmid replicon typing and conjugation experiment of S . enterica serovar Indiana isolates Plasmids were isolated using Plasmid Plus Midi kit (Qiagen, Germany). .. Replicon typing of plasmids was performed using a PCR-based method as previously described [ ].

    Article Title: Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi
    Article Snippet: .. DNA minipreps were done using Zyppy plasmid miniprep kit (Zymo Research), while midipreps were done using the Plasmid Plus midi kit (Qiagen) from 50 ml of overnight E. coli cultures in Super Broth. .. Correct insert DNA sequences were confirmed at Quintarabio or using an in-house Sanger DNA sequencing service at the Yale Keck Biotechnology Resource Laboratory.

    Article Title: Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity
    Article Snippet: .. Following ligation, plasmids were transformed into chemically competent Top10 cells, and plasmids were extracted and purified using the QIAamp DNA Mini or Plasmid Plus Midi Kit (all from Qiagen). .. Cell culture Before use, all cell lines were authenticated and tested negative for mycoplasma contamination via a commercial service (Multiplexion, Heidelberg).

    Article Title: Human Serum Albumin-Based Nanoparticle-Mediated In Vitro Gene Delivery
    Article Snippet: .. Bacterial colonies were amplified in ampicillin-containing medium, and the plasmid cDNA was purified using the QIAGEN Plasmid Plus Midi Kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s high yield protocol. .. The plasmid content was determined with a Nanodrop 2000™ spectrophotometer (Thermo Scientific, Wilmington, USA), and the plasmid cDNA stocks were stored at −20°C.

    Article Title: Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme
    Article Snippet: .. The cloned plasmid was isolated from E. coli using Mini or Midi column plasmid purification kits (Qiagen, Inc., Santa Clarita, Calif.). .. Restriction enzyme digestion (visualized on a 0.8% ethidium bromide agarose gel) and DNA sequence analysis of the plasmid indicated that pepO was in frame and in a proper orientation to allow for the production of the fusion protein.

    Article Title: Immunogenicity and Protection Efficacy of a Naked Self-Replicating mRNA-Based Zika Virus Vaccine
    Article Snippet: .. After 16 h, the bacteria were lysed and the plasmids were purified using the Plasmid Plus Midi kit (Qiagen, Germany). .. Subsequently, the plasmids were linearized with I-SceI endonuclease (NEB, Massachusetts, USA) and sr-mRNA were synthetized by IVT with the MEGAscript T7 Transcription kit (Life Technologies, Massachusetts, USA).

    Article Title: Random and targeted transgene insertion in C. elegans using a modified Mosl transposon
    Article Snippet: .. We isolated DNA with Spin Miniprep (cat. no. 27106) and Plasmid Plus Midiprep (cat. no. 12943) kits from Qiagen and with a PureLink HQ Mini Plasmid kit from Invitrogen (cat. no. K2100-01). .. The higher quality DNA kits (Qiagen Midi and Invitrogen Mini kits) resulted in a four-fold increase in F1-rescued animals (20 vs 5 rescued animals per injection) and a 50% (Qiagen Midi) to 100% (Invitrogen mini) increase in mosSCI insertion frequency ( ).

    Article Title: Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii
    Article Snippet: .. Plasmid vectors for microinjections were prepared using NucleoSpin® Plasmid Transfection-grade (Macherey-Nagel) or QIAGEN Plasmid Plus Midi Kit (QIAGEN GmbH, 40,724 Hilden, Germany). .. Fly strain and husbandry All fly experiments were performed in our well-equipped safety level one (S1) laboratory, which is certified for generating and using genetically modified insects.

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    https://www.bioz.com/result/plasmid plus midi kit/product/Qiagen
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      Buy from Supplier

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