plasmid midi kit  (Qiagen)


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    Structured Review

    Qiagen plasmid midi kit
    Plasmid Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid midi kit/product/Qiagen
    Average 99 stars, based on 1055 article reviews
    Price from $9.99 to $1999.99
    plasmid midi kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: The cloned influenza B fragment was a 371 bp region of the influenza B isolate B/Victoria/102/85, amplified by primers 5 AGG AAC GCT CTG TGC TTT GTG 3 and 5 TCT TTG GCT TGG ATT TCT 3 . .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    RNA Extraction:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: At least four calibration standards containing a known copy number of virus were included on each plate to indicate any changes in the efficiency of the viral RNA extraction and RT reaction. .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Amplification:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen). .. Plasmid insert DNA sequences were verified by sequencing in both directions using dye-labelled dideoxy-terminator cycle sequencing.

    In Vitro:

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: Other mutants, pUBRC1-p88u, pUBRC1-871A, pUBRC1-871G, pUBRC1-871C, and pUBRC1-p88uGVD were created by PCR-based in vitro mutagenesis from pUBRC1. .. All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN).

    Ligation:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: Real-time qPCR protocol Separate plasmids containing Influenza A and B M1 derived inserts that included the real time qPCR assay amplicons were constructed by ligation of a PCR amplified matrix gene fragment in pCRII according to the instructions of the T/A Cloning ® Kit Dual Promotor (Invitrogen, Groningen, The Netherlands). .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Mutagenesis:

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: In short, recombinant PCR products were digested by appropriate restriction enzymes that had unique recognition sites neighboring the mutation sites in pUBRC1, and the fragments containing the mutation substituted the corresponding fragments in pUBRC1. .. All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN).

    Synthesized:

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN). .. RNA transcripts were synthesized from these plasmids by T7 RNA polymerase after linearization by XmaI.

    Construct:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: Real-time qPCR protocol Separate plasmids containing Influenza A and B M1 derived inserts that included the real time qPCR assay amplicons were constructed by ligation of a PCR amplified matrix gene fragment in pCRII according to the instructions of the T/A Cloning ® Kit Dual Promotor (Invitrogen, Groningen, The Netherlands). .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Purification:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen). .. Plasmid insert DNA sequences were verified by sequencing in both directions using dye-labelled dideoxy-terminator cycle sequencing.

    Real-time Polymerase Chain Reaction:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: Paragraph title: Real-time qPCR protocol ... The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: Real-time qPCR protocol Separate plasmids containing Influenza A and B M1 derived inserts that included the real time qPCR assay amplicons were constructed by ligation of a PCR amplified matrix gene fragment in pCRII according to the instructions of the T/A Cloning ® Kit Dual Promotor (Invitrogen, Groningen, The Netherlands). .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: In short, recombinant PCR products were digested by appropriate restriction enzymes that had unique recognition sites neighboring the mutation sites in pUBRC1, and the fragments containing the mutation substituted the corresponding fragments in pUBRC1. .. All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN).

    Plasmid Preparation:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen). .. Plasmid insert DNA sequences were verified by sequencing in both directions using dye-labelled dideoxy-terminator cycle sequencing.

    Article Title: Macrophages from disease resistant B2 haplotype chickens activate T lymphocytes more effectively than macrophages from disease susceptible B19 birds.
    Article Snippet: .. Vaccination of chicks The NP of the AIV plasmid was prepared as described by Singh et al. (2010a) using the Qiagen Plasmid Midi kit according to manufacturer's instructions. .. Chicks were vaccinated intramuscularly (i.m.) with 500 mg of cDNA expressing the NP cDNA plasmid (Singh et al., 2010a) .

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: .. All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN). .. The plasmids pUCR1-871A, pUCR1-871A-dCP, pUCR1-p88u and pUCR1-p88u-dCP were created by replacement of the Aor51HI-BsiWI region in pUCR1 (Takeda et al., 2005) with the corresponding regions from pUBRC1-871A, pUBRC1-871-dCP, pUBRC1-p88u and pUBRC1-p88u-dCP, respectively.

    Expressing:

    Article Title: Macrophages from disease resistant B2 haplotype chickens activate T lymphocytes more effectively than macrophages from disease susceptible B19 birds.
    Article Snippet: Vaccination of chicks The NP of the AIV plasmid was prepared as described by Singh et al. (2010a) using the Qiagen Plasmid Midi kit according to manufacturer's instructions. .. Chicks were vaccinated intramuscularly (i.m.) with 500 mg of cDNA expressing the NP cDNA plasmid (Singh et al., 2010a) .

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: The plasmids for protein expression, pUBp27 and pUBp88 were previously described (Takeda et al., 2005) . .. All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN).

    Sequencing:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen). .. Plasmid insert DNA sequences were verified by sequencing in both directions using dye-labelled dideoxy-terminator cycle sequencing.

    Concentration Assay:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: The copy number of viral cDNA in copies/ml virus transport medium was determined for influenza A and B by comparison with a serially diluted plasmid standard of known concentration included on each 96 well plate. .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Recombinant:

    Article Title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
    Article Snippet: In short, recombinant PCR products were digested by appropriate restriction enzymes that had unique recognition sites neighboring the mutation sites in pUBRC1, and the fragments containing the mutation substituted the corresponding fragments in pUBRC1. .. All infectious DNA plasmids were prepared by using QIAGEN Plasmid Midi Kit (QIAGEN).

    Derivative Assay:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: Real-time qPCR protocol Separate plasmids containing Influenza A and B M1 derived inserts that included the real time qPCR assay amplicons were constructed by ligation of a PCR amplified matrix gene fragment in pCRII according to the instructions of the T/A Cloning ® Kit Dual Promotor (Invitrogen, Groningen, The Netherlands). .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.
    Article Snippet: The cloned influenza A fragment comprised the entire M1 protein gene and was obtained by RT-PCR from stocks of A/Texas/1/77 (H1N1). .. The plasmid DNA was amplified in E. coli strain TOP10 according to the manufacturer's protocol and purified using a Qiagen Plasmid Midi kit (Qiagen).

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    Qiagen genomic dna buffer set
    Complete genome sequence of <t>Ureaplasma</t> OMC-P162 strain and <t>DNA</t> modification. (A) The distribution of genes was depicted on the two outermost concentric circles. First concentric circle: predicted coding DNA sequences (CDSs) on the plus strand. Second concentric circle: predicted CDSs on the minus strand. The gray portion of the middle circle represents the nucleotides sequences while the pink portion indicated tRNA and rRNA. The innermost circle represents the GC skew. This figure was generated using DNAPlotter: Release 1.10 from Artemis Release 15.0.0, Sanger Institute. CDSs were classified by KEGG pathway categories and color codes for KEGG pathway categories were shown in the right. The genome size, GC content and numbers of CDS, rRNA and tRNA were also shown. (B) The upper panel shows the coverage of sequence reads and the bottom two panels show modification types and numbers of modification events within 2 kb of the genome of the plus (+) and minus strands (−).
    Genomic Dna Buffer Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna buffer set/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna buffer set - by Bioz Stars, 2020-04
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    99
    Qiagen cell culture dna midi kit
    Elements used in construction of the polypeptide secretion library of S . aureus in E. coli . A. Expression vector pSRP18/0 contains an expression cassette comprised of a 5' untranslated sequence upstream of the flagellin gene of E. coli MG1655 ( fliC MG1655 ) here indicated fliC 5'UTR, a <t>DNA</t> fragment encoding the N-terminal 20 amino acids fliC MG1655 ( fliC 1-60), a synthetic FLAG tag encoding sequence ( flag ) and a 3' untranslated region downstream of fliC MG1655 ( fliC 3'UTR). Eco RV indicates the unique cloning site for foreign DNA fragments, horizontal arrows indicate the oligonucleotides used as primers for PCR (017F, 025F and 028R) and sequencing (017F and 071R) of the cloned inserts and black lines indicate sequences of the plasmid pBR322. Sal I and Bam HI indicate the restriction sites created during cloning of the expression cassette into pBR322. B. Agarose gel electrophoretic analysis of the chromosomal DNA isolated from S. aureus <t>NCTC</t> 8325-4 and used in generation of the library. The purified DNA is shown in the left lane, randomly fragmented and blunted DNA in the right lane. Ns indicate not sonicated and s stands for sonicated and polished DNA. The positions of molecular weight markers in base pairs are shown to the right.
    Cell Culture Dna Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture dna midi kit/product/Qiagen
    Average 99 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    cell culture dna midi kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen plasmid dna midi kit
    Schematic representation of the class 1 integron-containing bla IMP-16 gene cassette from clinical isolate P. <t>aeruginosa</t> 101-4704. Boxes, inserted genes; arrows, transcriptional orientations; black circles, 59-be's; white circle, attI1 recombination site; lines, <t>DNA</t> of the inserts contained within recombinant plasmids pREM-1, pREM-2, pREM-3, and pREM-4; arrowheads, primer positions and their orientations; M, start codon; asterisk, the location of the stop codon for the particular gene.
    Plasmid Dna Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna midi kit/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    plasmid dna midi kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Complete genome sequence of Ureaplasma OMC-P162 strain and DNA modification. (A) The distribution of genes was depicted on the two outermost concentric circles. First concentric circle: predicted coding DNA sequences (CDSs) on the plus strand. Second concentric circle: predicted CDSs on the minus strand. The gray portion of the middle circle represents the nucleotides sequences while the pink portion indicated tRNA and rRNA. The innermost circle represents the GC skew. This figure was generated using DNAPlotter: Release 1.10 from Artemis Release 15.0.0, Sanger Institute. CDSs were classified by KEGG pathway categories and color codes for KEGG pathway categories were shown in the right. The genome size, GC content and numbers of CDS, rRNA and tRNA were also shown. (B) The upper panel shows the coverage of sequence reads and the bottom two panels show modification types and numbers of modification events within 2 kb of the genome of the plus (+) and minus strands (−).

    Journal: PLoS ONE

    Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain

    doi: 10.1371/journal.pone.0205328

    Figure Lengend Snippet: Complete genome sequence of Ureaplasma OMC-P162 strain and DNA modification. (A) The distribution of genes was depicted on the two outermost concentric circles. First concentric circle: predicted coding DNA sequences (CDSs) on the plus strand. Second concentric circle: predicted CDSs on the minus strand. The gray portion of the middle circle represents the nucleotides sequences while the pink portion indicated tRNA and rRNA. The innermost circle represents the GC skew. This figure was generated using DNAPlotter: Release 1.10 from Artemis Release 15.0.0, Sanger Institute. CDSs were classified by KEGG pathway categories and color codes for KEGG pathway categories were shown in the right. The genome size, GC content and numbers of CDS, rRNA and tRNA were also shown. (B) The upper panel shows the coverage of sequence reads and the bottom two panels show modification types and numbers of modification events within 2 kb of the genome of the plus (+) and minus strands (−).

    Article Snippet: DNA extraction, plasmid construction and PCR mutagenesis Ureaplasma genomic DNA was prepared by Genomic DNA Buffer Set (Qiagen, Hilden, Germany) and isolated by QIAGEN Genomic-tip 20/G (Qiagen, Hilden, Germany), which was then amplified by polymerase chain reaction (PCR) using Ex Taq (Takara Bio, Shiga, Japan) for plasmid construction.

    Techniques: Sequencing, Modification, Generated

    DNA digestion by Upa P162 and Nla III. (A) The indicated Ureaplasma genomes were treated with either Upa P162 or Nla III for 1 h. (B) UPV229-treated and untreated pT7Blue plasmid was digested by either Upa P162 or Nla III for 1 h.

    Journal: PLoS ONE

    Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain

    doi: 10.1371/journal.pone.0205328

    Figure Lengend Snippet: DNA digestion by Upa P162 and Nla III. (A) The indicated Ureaplasma genomes were treated with either Upa P162 or Nla III for 1 h. (B) UPV229-treated and untreated pT7Blue plasmid was digested by either Upa P162 or Nla III for 1 h.

    Article Snippet: DNA extraction, plasmid construction and PCR mutagenesis Ureaplasma genomic DNA was prepared by Genomic DNA Buffer Set (Qiagen, Hilden, Germany) and isolated by QIAGEN Genomic-tip 20/G (Qiagen, Hilden, Germany), which was then amplified by polymerase chain reaction (PCR) using Ex Taq (Takara Bio, Shiga, Japan) for plasmid construction.

    Techniques: Plasmid Preparation

    Complete genome sequence of Ureaplasma OMC-P162 strain and DNA modification. (A) The distribution of genes was depicted on the two outermost concentric circles. First concentric circle: predicted coding DNA sequences (CDSs) on the plus strand. Second concentric circle: predicted CDSs on the minus strand. The gray portion of the middle circle represents the nucleotides sequences while the pink portion indicated tRNA and rRNA. The innermost circle represents the GC skew. This figure was generated using DNAPlotter: Release 1.10 from Artemis Release 15.0.0, Sanger Institute. CDSs were classified by KEGG pathway categories and color codes for KEGG pathway categories were shown in the right. The genome size, GC content and numbers of CDS, rRNA and tRNA were also shown. (B) The upper panel shows the coverage of sequence reads and the bottom two panels show modification types and numbers of modification events within 2 kb of the genome of the plus (+) and minus strands (−).

    Journal: PLoS ONE

    Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain

    doi: 10.1371/journal.pone.0205328

    Figure Lengend Snippet: Complete genome sequence of Ureaplasma OMC-P162 strain and DNA modification. (A) The distribution of genes was depicted on the two outermost concentric circles. First concentric circle: predicted coding DNA sequences (CDSs) on the plus strand. Second concentric circle: predicted CDSs on the minus strand. The gray portion of the middle circle represents the nucleotides sequences while the pink portion indicated tRNA and rRNA. The innermost circle represents the GC skew. This figure was generated using DNAPlotter: Release 1.10 from Artemis Release 15.0.0, Sanger Institute. CDSs were classified by KEGG pathway categories and color codes for KEGG pathway categories were shown in the right. The genome size, GC content and numbers of CDS, rRNA and tRNA were also shown. (B) The upper panel shows the coverage of sequence reads and the bottom two panels show modification types and numbers of modification events within 2 kb of the genome of the plus (+) and minus strands (−).

    Article Snippet: Ureaplasma genomic DNA was prepared by Genomic DNA Buffer Set (Qiagen, Hilden, Germany) and isolated by QIAGEN Genomic-tip 20/G (Qiagen, Hilden, Germany), which was then amplified by polymerase chain reaction (PCR) using Ex Taq (Takara Bio, Shiga, Japan) for plasmid construction.

    Techniques: Sequencing, Modification, Generated

    DNA digestion by Upa P162 and Nla III. (A) The indicated Ureaplasma genomes were treated with either Upa P162 or Nla III for 1 h. (B) UPV229-treated and untreated pT7Blue plasmid was digested by either Upa P162 or Nla III for 1 h.

    Journal: PLoS ONE

    Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain

    doi: 10.1371/journal.pone.0205328

    Figure Lengend Snippet: DNA digestion by Upa P162 and Nla III. (A) The indicated Ureaplasma genomes were treated with either Upa P162 or Nla III for 1 h. (B) UPV229-treated and untreated pT7Blue plasmid was digested by either Upa P162 or Nla III for 1 h.

    Article Snippet: Ureaplasma genomic DNA was prepared by Genomic DNA Buffer Set (Qiagen, Hilden, Germany) and isolated by QIAGEN Genomic-tip 20/G (Qiagen, Hilden, Germany), which was then amplified by polymerase chain reaction (PCR) using Ex Taq (Takara Bio, Shiga, Japan) for plasmid construction.

    Techniques: Plasmid Preparation

    Elements used in construction of the polypeptide secretion library of S . aureus in E. coli . A. Expression vector pSRP18/0 contains an expression cassette comprised of a 5' untranslated sequence upstream of the flagellin gene of E. coli MG1655 ( fliC MG1655 ) here indicated fliC 5'UTR, a DNA fragment encoding the N-terminal 20 amino acids fliC MG1655 ( fliC 1-60), a synthetic FLAG tag encoding sequence ( flag ) and a 3' untranslated region downstream of fliC MG1655 ( fliC 3'UTR). Eco RV indicates the unique cloning site for foreign DNA fragments, horizontal arrows indicate the oligonucleotides used as primers for PCR (017F, 025F and 028R) and sequencing (017F and 071R) of the cloned inserts and black lines indicate sequences of the plasmid pBR322. Sal I and Bam HI indicate the restriction sites created during cloning of the expression cassette into pBR322. B. Agarose gel electrophoretic analysis of the chromosomal DNA isolated from S. aureus NCTC 8325-4 and used in generation of the library. The purified DNA is shown in the left lane, randomly fragmented and blunted DNA in the right lane. Ns indicate not sonicated and s stands for sonicated and polished DNA. The positions of molecular weight markers in base pairs are shown to the right.

    Journal: BMC Microbiology

    Article Title: Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli

    doi: 10.1186/1471-2180-11-117

    Figure Lengend Snippet: Elements used in construction of the polypeptide secretion library of S . aureus in E. coli . A. Expression vector pSRP18/0 contains an expression cassette comprised of a 5' untranslated sequence upstream of the flagellin gene of E. coli MG1655 ( fliC MG1655 ) here indicated fliC 5'UTR, a DNA fragment encoding the N-terminal 20 amino acids fliC MG1655 ( fliC 1-60), a synthetic FLAG tag encoding sequence ( flag ) and a 3' untranslated region downstream of fliC MG1655 ( fliC 3'UTR). Eco RV indicates the unique cloning site for foreign DNA fragments, horizontal arrows indicate the oligonucleotides used as primers for PCR (017F, 025F and 028R) and sequencing (017F and 071R) of the cloned inserts and black lines indicate sequences of the plasmid pBR322. Sal I and Bam HI indicate the restriction sites created during cloning of the expression cassette into pBR322. B. Agarose gel electrophoretic analysis of the chromosomal DNA isolated from S. aureus NCTC 8325-4 and used in generation of the library. The purified DNA is shown in the left lane, randomly fragmented and blunted DNA in the right lane. Ns indicate not sonicated and s stands for sonicated and polished DNA. The positions of molecular weight markers in base pairs are shown to the right.

    Article Snippet: Construction of the primary genomic library Chromosomal DNA from S. aureus NCTC 8325-4 was purified using Blood and cell culture DNA Midi Kit with genomic-tip 100/G (Qiagen) and randomly fragmented by ultrasonic treatment (4 sec., Ultrasonic processor, VCX600) into fragments of mainly 250 to 1000 bp in length.

    Techniques: Expressing, Plasmid Preparation, Sequencing, FLAG-tag, Clone Assay, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Isolation, Purification, Sonication, Molecular Weight

    Schematic representation of the class 1 integron-containing bla IMP-16 gene cassette from clinical isolate P. aeruginosa 101-4704. Boxes, inserted genes; arrows, transcriptional orientations; black circles, 59-be's; white circle, attI1 recombination site; lines, DNA of the inserts contained within recombinant plasmids pREM-1, pREM-2, pREM-3, and pREM-4; arrowheads, primer positions and their orientations; M, start codon; asterisk, the location of the stop codon for the particular gene.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Integron Carrying a Novel Metallo-?-Lactamase Gene, blaIMP-16, and a Fused Form of Aminoglycoside-Resistant Gene aac(6′)-30/aac(6′)-Ib′: Report from the SENTRY Antimicrobial Surveillance Program

    doi: 10.1128/AAC.48.12.4693-4702.2004

    Figure Lengend Snippet: Schematic representation of the class 1 integron-containing bla IMP-16 gene cassette from clinical isolate P. aeruginosa 101-4704. Boxes, inserted genes; arrows, transcriptional orientations; black circles, 59-be's; white circle, attI1 recombination site; lines, DNA of the inserts contained within recombinant plasmids pREM-1, pREM-2, pREM-3, and pREM-4; arrowheads, primer positions and their orientations; M, start codon; asterisk, the location of the stop codon for the particular gene.

    Article Snippet: Plasmid DNA from P. aeruginosa 101-4704 was extracted with a plasmid DNA Midi kit (Qiagen, Chatsworth, Calif.).

    Techniques: Recombinant