plasmid maxi dna purification kit  (Qiagen)


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    QIAGEN Plasmid Maxi Kit
    Description:
    For purification of up to 10 mg transfection grade plasmid or cosmid DNA Kit contents Qiagen Plasmid Maxi Kit 10 preps 100 to 500mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology 500g Yield 160 min prep Run Manual Processing For Transfection Cloning Sequencing Capillary Sequencing Includes 25 Qiagen to tip 10 110mL Buffer P1 Buffer P2 Buffer P3 Buffer QBT 3 x 240mL Buffer QC 170mL Buffer QF 11mg RNase A 100μL LyseBlue Benefits Purity equivalent to 2 x CsCl gradient centrifugation High yields of plasmid DNA Cost effective preparations LyseBlue for optimum lysis and maximum DNA yie
    Catalog Number:
    12162
    Price:
    249
    Category:
    QIAGEN Plasmid Kits
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    Structured Review

    Qiagen plasmid maxi dna purification kit
    QIAGEN Plasmid Maxi Kit
    For purification of up to 10 mg transfection grade plasmid or cosmid DNA Kit contents Qiagen Plasmid Maxi Kit 10 preps 100 to 500mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology 500g Yield 160 min prep Run Manual Processing For Transfection Cloning Sequencing Capillary Sequencing Includes 25 Qiagen to tip 10 110mL Buffer P1 Buffer P2 Buffer P3 Buffer QBT 3 x 240mL Buffer QC 170mL Buffer QF 11mg RNase A 100μL LyseBlue Benefits Purity equivalent to 2 x CsCl gradient centrifugation High yields of plasmid DNA Cost effective preparations LyseBlue for optimum lysis and maximum DNA yie
    https://www.bioz.com/result/plasmid maxi dna purification kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid maxi dna purification kit - by Bioz Stars, 2020-04
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    Related Articles

    Transduction:

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: Paragraph title: Lentiviral vector production and cell transduction ... Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions.

    Clone Assay:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: The DNA constructs were cloned first by PCR using a Topo TA vector (Invitrogen), then digested with SalI and NotI and ligated into SalI and NotI sites in the plasmid vector VRC8400 pCMV/R (a generous donation of G. Nabel, NIH Vaccine Research Center) containing the enhanced human cytomegalovirus promoter CMV/R [ ]. .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame. .. HA-ubiquitin wild-type (HA-Ub wt) and HA-Ub chains mutated at Lys-48 or Lys-63, HA-Ub K48R, and HA-Ub K63R, respectively, cloned in pcDNA3 vectors, were gifts from Drs I. Dikic and K. Haglund (Goethe University, Frankfurt, Germany; Ref. ).

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: The recombinant plasmid was sequenced to ensure the insert sequence was cloned correctly. .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol.

    Amplification:

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: The amplified product was inserted as a BamHI–XbaI fragment into the corresponding sites of the pcDNA3 expression vector. .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien).

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: The entire SjTGR open reading fragment was amplified from the S. japonicum adult worm cDNA library, with primers: 5′-CGCGGATCC ATGCCTCCGATTGAT-3′ and 5′-GCCTCGAG TCAGCAACCGGTTACC-3′ and subcloned into cleaved pVAX1 to construct the recombinant expression plasmid, pVAX1/SjTGR. .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol.

    Polymerase Chain Reaction:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: The DNA constructs were cloned first by PCR using a Topo TA vector (Invitrogen), then digested with SalI and NotI and ligated into SalI and NotI sites in the plasmid vector VRC8400 pCMV/R (a generous donation of G. Nabel, NIH Vaccine Research Center) containing the enhanced human cytomegalovirus promoter CMV/R [ ]. .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Construct:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: Paragraph title: Design and expression of sCD4-17b variants and related protein constructs ... DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: Gene delivery into hepatic cells with ternary complexes of plasmid DNA, cationic liposomes and apolipoprotein E-derived peptide
    Article Snippet: Preparation of plasmid DNA The pCMV-luc plasmid encoding the firefly luciferase gene under the control of the cytomegalovirus (CMV) promoter was constructed as described previously ( ). .. A protein-free preparation of pDNA was purified after alkaline lysis using a QIAGEN Plasmid Maxi Kit (Qiagen, Hilden, Germany).

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: For the generation of a Rem expression construct, the rem -coding sequence was amplified from cDNA obtained by reverse transcription of RNA extracted from the MMTV-producing murine cell line GR. .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien).

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: The entire SjTGR open reading fragment was amplified from the S. japonicum adult worm cDNA library, with primers: 5′-CGCGGATCC ATGCCTCCGATTGAT-3′ and 5′-GCCTCGAG TCAGCAACCGGTTACC-3′ and subcloned into cleaved pVAX1 to construct the recombinant expression plasmid, pVAX1/SjTGR. .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol.

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions. .. Briefly, HEK293T cells were co-transfected with VSV-G construct (pHCMV-G, kindly provided by Prof. Volker Erfle, Institut für Molekulare Virologie, Neuherberg, Germany), pCMVR8.74 (Addgene plasmid #22036, gift from Didier Trono, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland), and the plasmid of interest.

    Incubation:

    Article Title: Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame
    Article Snippet: Bacmid DNA was purified from 0.5 L cultures using the plasmid Maxi plasmid DNA purification kit (Qiagen). .. Bacmid DNA was mixed with 200 μl of Sf-900 II medium containing 10 μl of liposomes and incubated at 27°C for 30-45 minutes.

    Luciferase:

    Article Title: Gene delivery into hepatic cells with ternary complexes of plasmid DNA, cationic liposomes and apolipoprotein E-derived peptide
    Article Snippet: Preparation of plasmid DNA The pCMV-luc plasmid encoding the firefly luciferase gene under the control of the cytomegalovirus (CMV) promoter was constructed as described previously ( ). .. A protein-free preparation of pDNA was purified after alkaline lysis using a QIAGEN Plasmid Maxi Kit (Qiagen, Hilden, Germany).

    Expressing:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: Paragraph title: Design and expression of sCD4-17b variants and related protein constructs ... DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters
    Article Snippet: Also, there were no differences in level of GPF expression when both plasmid preparations were transfected into 293T cells using either calcium phosphate precipitation or lipofectamine . .. Therefore, plasmid purified using glass syringe filters appears to be of the same quality as plasmid DNA purified using a Qiagen Maxi-prep kit.

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: The amplified product was inserted as a BamHI–XbaI fragment into the corresponding sites of the pcDNA3 expression vector. .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien).

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol. .. Expression in 293T Cells Transfections of plasmid (pVAX1/SjTGR, pVAX1) were done by Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol to detect gene expression in 293T cells.

    Modification:

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame. .. SV40-transformed African green monkey kidney cells (COS-1) and Chinese hamster ovarian (CHO)-KI cells were grown in Dulbecco′s modified Eagle′s medium (Sigma), 4 m m l -glutamine, 100 IU/ml penicillin, 100 μg/ml streptomycin, and 10% fetal calf serum (Invitrogen; Complete medium).

    Western Blot:

    Article Title: Sequences Downstream of the Translation Initiation Codon Are Important Determinants of Translation Efficiency in Chloroplasts 1
    Article Snippet: DNA for plastid transformation was prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Valencia, CA). .. A uniform population of transformed plastid genome copies was confirmed by DNA gel-blot analysis.

    Transformation Assay:

    Article Title: Sequences Downstream of the Translation Initiation Codon Are Important Determinants of Translation Efficiency in Chloroplasts 1
    Article Snippet: .. DNA for plastid transformation was prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Valencia, CA). ..

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: Plasmids were transformed into E. coli One Shot Top10 cells (Invitrogen) and grown under kanamycin selection. .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: One Shot™ Stbl3™ chemically competent E. coli (Invitrogen, Thermo Fisher Scientific) were transformed by heat shock and chloramphenicol-selected (Sigma Aldrich). .. Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions.

    Over Expression:

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: Lentiviral vector production and cell transduction For membrane-associated SCF overexpression, pLenti-C-mGFP-P2A-Puro plasmid encoding GFP-tagged human KIT ligand, transcript variant “a”, as well as the empty vector, were purchased from OriGene Technologies (Rockville, MD). .. Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions.

    Derivative Assay:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: To enhance expression and subsequent purification, all variants contained the N-terminal leader sequence of human Ig kappa light chain, and a 9 amino acid C-terminal epitope tag derived from the intracellular C-terminus of bovine rhodopsin (designated C9, with the following sequence: TETSQVAPA) that is recognized by the rho 1D4 MAb [ ] (herein referred to as 1D4). .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: The HIV-1 Rev expression construct pCMVsRevBF was derived from the plasmid pCMVsrev (a kind gift from B. Felber) as previously described ( , ). .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien).

    Transfection:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: DNA was prepared using a Plasmid Maxi Kit (Qiagen). .. Proteins were expressed by transient transfection of re-adherent FreeSyle 293F cells (Invitrogen) using Fugene (Roche) according to manufacturer's instructions.

    Article Title: Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame
    Article Snippet: Paragraph title: Bacmid purification and transfection ... Bacmid DNA was purified from 0.5 L cultures using the plasmid Maxi plasmid DNA purification kit (Qiagen).

    Article Title: A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters
    Article Snippet: Also, there were no differences in level of GPF expression when both plasmid preparations were transfected into 293T cells using either calcium phosphate precipitation or lipofectamine . .. Therefore, plasmid purified using glass syringe filters appears to be of the same quality as plasmid DNA purified using a Qiagen Maxi-prep kit.

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien). .. Cell culture and transfection The MMTV permissive feline kidney cell line, CrFK, was maintained in Dulbecco's modified Eagle's medium supplemented with 10% heat inactivated fetal calf serum ( ).

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: Paragraph title: Construction of Transcripts, Cell Culture, and Transfection ... Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame.

    Ligation:

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: The cDNAs encoding the open reading frames of mouse HAS2 and HAS3 genes in pcNeoI plasmid ( , ), kindly provided by Dr. A. Spicer, were excised by XhoI/XbaI digestions followed by ligation in Flag-, 6myc-, or HA-pcDNA3 vectors, fused in-frame at their N termini. .. Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame.

    Cell Culture:

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: Paragraph title: Construction of Transcripts, Cell Culture, and Transfection ... Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame.

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: .. Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions. .. Lentiviral vector stocks were generated by a transient three-plasmid vector packaging system.

    Generated:

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions. .. Lentiviral vector stocks were generated by a transient three-plasmid vector packaging system.

    Sequencing:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: Constructs representing the individual moieties (sCD4 and 17b scFV) were also prepared, each containing the same N-terminal leader sequence and C-terminal C9 epitope tag. .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: For the generation of a Rem expression construct, the rem -coding sequence was amplified from cDNA obtained by reverse transcription of RNA extracted from the MMTV-producing murine cell line GR. .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien).

    Recombinant:

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: Paragraph title: 2.2. Construction of Recombinant Plasmid DNA ... Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol.

    Labeling:

    Article Title: Expanding the Genetic Code of Escherichia coli with Phosphotyrosine
    Article Snippet: Paragraph title: tRNA transcription, purification, and 32 P labeling ... Template plasmids containing tRNA genes were purified with the plasmid maxi kit (Qiagen), and 100 µg of plasmid was digested with BstNI (New England Biolabs).

    Purification:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: To enhance expression and subsequent purification, all variants contained the N-terminal leader sequence of human Ig kappa light chain, and a 9 amino acid C-terminal epitope tag derived from the intracellular C-terminus of bovine rhodopsin (designated C9, with the following sequence: TETSQVAPA) that is recognized by the rho 1D4 MAb [ ] (herein referred to as 1D4). .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Article Title: Gene delivery into hepatic cells with ternary complexes of plasmid DNA, cationic liposomes and apolipoprotein E-derived peptide
    Article Snippet: .. A protein-free preparation of pDNA was purified after alkaline lysis using a QIAGEN Plasmid Maxi Kit (Qiagen, Hilden, Germany). ..

    Article Title: Expanding the Genetic Code of Escherichia coli with Phosphotyrosine
    Article Snippet: .. Template plasmids containing tRNA genes were purified with the plasmid maxi kit (Qiagen), and 100 µg of plasmid was digested with BstNI (New England Biolabs). .. The BstNI digested template DNA was purified by phenol/chloroform extraction, followed by ethanol precipitation and dissolved in double distilled water.

    Article Title: Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame
    Article Snippet: .. Bacmid DNA was purified from 0.5 L cultures using the plasmid Maxi plasmid DNA purification kit (Qiagen). .. Equimolar amounts (~2 μg) of purified bacmid DNA were transfected into Sf-9 cells (0.9 × 106 /well) seeded in a six-well plate using a cationic liposome method ( ).

    Article Title: A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters
    Article Snippet: .. Therefore, plasmid purified using glass syringe filters appears to be of the same quality as plasmid DNA purified using a Qiagen Maxi-prep kit. .. Comparison of Methods for DNA Isolation In order to further compare the quantity and quality of plasmid DNA purified by glass syringe filter with that of DNA purified using the Qiagen Maxiprep kit, we purified three plasmids (pEGFP-N1, pLL3.7 and pLL3-LS) using both methods.

    Article Title: A new toolset for protein expression and subcellular localization studies in citrus and its application to citrus tristeza virus proteins
    Article Snippet: .. For bombardment, all plasmids were first purified from E. coli with the Qiagen plasmid maxi-kit (Qiagen, Hilden, Germany). ..

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: .. Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame. .. HA-ubiquitin wild-type (HA-Ub wt) and HA-Ub chains mutated at Lys-48 or Lys-63, HA-Ub K48R, and HA-Ub K63R, respectively, cloned in pcDNA3 vectors, were gifts from Drs I. Dikic and K. Haglund (Goethe University, Frankfurt, Germany; Ref. ).

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol. .. Expression in 293T Cells Transfections of plasmid (pVAX1/SjTGR, pVAX1) were done by Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol to detect gene expression in 293T cells.

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: .. Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions. .. Lentiviral vector stocks were generated by a transient three-plasmid vector packaging system.

    Transgenic Assay:

    Article Title: Sequences Downstream of the Translation Initiation Codon Are Important Determinants of Translation Efficiency in Chloroplasts 1
    Article Snippet: Paragraph title: Plastid Transformation and Regeneration of Transgenic Plants ... DNA for plastid transformation was prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Valencia, CA).

    cDNA Library Assay:

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: The entire SjTGR open reading fragment was amplified from the S. japonicum adult worm cDNA library, with primers: 5′-CGCGGATCC ATGCCTCCGATTGAT-3′ and 5′-GCCTCGAG TCAGCAACCGGTTACC-3′ and subcloned into cleaved pVAX1 to construct the recombinant expression plasmid, pVAX1/SjTGR. .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol.

    Plasmid Preparation:

    Article Title: Sequences Downstream of the Translation Initiation Codon Are Important Determinants of Translation Efficiency in Chloroplasts 1
    Article Snippet: .. DNA for plastid transformation was prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Valencia, CA). ..

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: .. DNA was prepared using a Plasmid Maxi Kit (Qiagen). .. Proteins were expressed by transient transfection of re-adherent FreeSyle 293F cells (Invitrogen) using Fugene (Roche) according to manufacturer's instructions.

    Article Title: Gene delivery into hepatic cells with ternary complexes of plasmid DNA, cationic liposomes and apolipoprotein E-derived peptide
    Article Snippet: .. A protein-free preparation of pDNA was purified after alkaline lysis using a QIAGEN Plasmid Maxi Kit (Qiagen, Hilden, Germany). ..

    Article Title: Expanding the Genetic Code of Escherichia coli with Phosphotyrosine
    Article Snippet: .. Template plasmids containing tRNA genes were purified with the plasmid maxi kit (Qiagen), and 100 µg of plasmid was digested with BstNI (New England Biolabs). .. The BstNI digested template DNA was purified by phenol/chloroform extraction, followed by ethanol precipitation and dissolved in double distilled water.

    Article Title: Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame
    Article Snippet: .. Bacmid DNA was purified from 0.5 L cultures using the plasmid Maxi plasmid DNA purification kit (Qiagen). .. Equimolar amounts (~2 μg) of purified bacmid DNA were transfected into Sf-9 cells (0.9 × 106 /well) seeded in a six-well plate using a cationic liposome method ( ).

    Article Title: A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters
    Article Snippet: .. Therefore, plasmid purified using glass syringe filters appears to be of the same quality as plasmid DNA purified using a Qiagen Maxi-prep kit. .. Comparison of Methods for DNA Isolation In order to further compare the quantity and quality of plasmid DNA purified by glass syringe filter with that of DNA purified using the Qiagen Maxiprep kit, we purified three plasmids (pEGFP-N1, pLL3.7 and pLL3-LS) using both methods.

    Article Title: Identification of the Rem-responsive element of mouse mammary tumor virus
    Article Snippet: .. Before transfections, plasmids were prepared using Plasmid Maxi Kit (Qiagen, Wien). .. Cell culture and transfection The MMTV permissive feline kidney cell line, CrFK, was maintained in Dulbecco's modified Eagle's medium supplemented with 10% heat inactivated fetal calf serum ( ).

    Article Title: A new toolset for protein expression and subcellular localization studies in citrus and its application to citrus tristeza virus proteins
    Article Snippet: .. For bombardment, all plasmids were first purified from E. coli with the Qiagen plasmid maxi-kit (Qiagen, Hilden, Germany). ..

    Article Title: The Activity of Hyaluronan Synthase 2 Is Regulated by Dimerization and Ubiquitination *
    Article Snippet: .. Plasmids were purified (Plasmid Maxi kit, Qiagen) and sequenced to confirm that the insertions were in-frame. .. HA-ubiquitin wild-type (HA-Ub wt) and HA-Ub chains mutated at Lys-48 or Lys-63, HA-Ub K48R, and HA-Ub K63R, respectively, cloned in pcDNA3 vectors, were gifts from Drs I. Dikic and K. Haglund (Goethe University, Frankfurt, Germany; Ref. ).

    Article Title: Strategies to Insulate Lentiviral Vector-Expressed Transgenes
    Article Snippet: .. QIAquick Gel Extraction Kit, QIAGEN Plasmid Maxi Kit (Qiagen). .. Replication-defective lentiviral vector particles ( ): produce and titer as described in other Chapters of this book.

    Article Title: Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum
    Article Snippet: .. Then, the expression plasmid was transferred into DH5α , a type of competent cell, for large-scale preparation and purification using a Qiagen Plasmid Maxi Kit (Qiagen), followed by the manufacturer's protocol. .. Expression in 293T Cells Transfections of plasmid (pVAX1/SjTGR, pVAX1) were done by Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol to detect gene expression in 293T cells.

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: .. Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions. .. Lentiviral vector stocks were generated by a transient three-plasmid vector packaging system.

    Selection:

    Article Title: sCD4-17b bifunctional protein: Extremely broad and potent neutralization of HIV-1 Env pseudotyped viruses from genetically diverse primary isolates
    Article Snippet: Plasmids were transformed into E. coli One Shot Top10 cells (Invitrogen) and grown under kanamycin selection. .. DNA was prepared using a Plasmid Maxi Kit (Qiagen).

    Ethanol Precipitation:

    Article Title: Expanding the Genetic Code of Escherichia coli with Phosphotyrosine
    Article Snippet: Template plasmids containing tRNA genes were purified with the plasmid maxi kit (Qiagen), and 100 µg of plasmid was digested with BstNI (New England Biolabs). .. The BstNI digested template DNA was purified by phenol/chloroform extraction, followed by ethanol precipitation and dissolved in double distilled water.

    Alkaline Lysis:

    Article Title: Gene delivery into hepatic cells with ternary complexes of plasmid DNA, cationic liposomes and apolipoprotein E-derived peptide
    Article Snippet: .. A protein-free preparation of pDNA was purified after alkaline lysis using a QIAGEN Plasmid Maxi Kit (Qiagen, Hilden, Germany). ..

    DNA Purification:

    Article Title: Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame
    Article Snippet: .. Bacmid DNA was purified from 0.5 L cultures using the plasmid Maxi plasmid DNA purification kit (Qiagen). .. Equimolar amounts (~2 μg) of purified bacmid DNA were transfected into Sf-9 cells (0.9 × 106 /well) seeded in a six-well plate using a cationic liposome method ( ).

    Gel Extraction:

    Article Title: Strategies to Insulate Lentiviral Vector-Expressed Transgenes
    Article Snippet: .. QIAquick Gel Extraction Kit, QIAGEN Plasmid Maxi Kit (Qiagen). .. Replication-defective lentiviral vector particles ( ): produce and titer as described in other Chapters of this book.

    Variant Assay:

    Article Title: A juxtacrine/paracrine loop between C-Kit and stem cell factor promotes cancer stem cell survival in epithelial ovarian cancer
    Article Snippet: Lentiviral vector production and cell transduction For membrane-associated SCF overexpression, pLenti-C-mGFP-P2A-Puro plasmid encoding GFP-tagged human KIT ligand, transcript variant “a”, as well as the empty vector, were purchased from OriGene Technologies (Rockville, MD). .. Bacteria were cultured in LB broth (Sigma Aldrich), and plasmids were purified by Plasmid Maxi Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions.

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  • 99
    Qiagen maxi plasmid dna purification kit
    Maxi Plasmid Dna Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxi plasmid dna purification kit/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    maxi plasmid dna purification kit - by Bioz Stars, 2020-04
    99/100 stars
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