plasmid maxi kit  (Qiagen)


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    Structured Review

    Qiagen plasmid maxi kit
    Plasmid Maxi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid maxi kit/product/Qiagen
    Average 99 stars, based on 744 article reviews
    Price from $9.99 to $1999.99
    plasmid maxi kit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: Paragraph title: Cloning and DNA sequencing ... Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: The clones were designated pN and pGAPDH, respectively. .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany).

    Transfection:

    Article Title: Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus.
    Article Snippet: Large scale preparation of pINA and pcPIL12 Escherichia coli strain DH5a (Invitrogen) was transfected with either pINA or pcPIL12 and grown in 1 L of LB medium supplemented with 100 mg/ml ampicillin (Sigma, St. Louis, MO) for 16 h at 37 8C with constant shaking ($300 rpm). .. Plasmid purification was conducted using a Qiagen Plasmid Maxi Kit (Qiagen Inc., Valencia, CA) according to manufacturer's instructions.

    Amplification:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: Cloning and DNA sequencing The PCR products amplified with each primer pair were purified using GENECLEAN Turbo kit (BIO101, La Jolla, CA, USA) according to the manufacturer's recommendation. .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    In Vitro:

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany). .. We transcribed 5 ml of each plasmid with T7 RNA polymerase from the Riboprobe † in vitro transcription system (Promega, Madison, USA) according to the manufacturer's instructions.

    Plasmid Preparation:

    Article Title: Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus.
    Article Snippet: .. Plasmid purification was conducted using a Qiagen Plasmid Maxi Kit (Qiagen Inc., Valencia, CA) according to manufacturer's instructions. .. Preparation of cartridges for intradermal biolistic delivery of cytokine cDNA Plasmid pINA or pcPIL12 was precipitated onto the surface of gold particles (average diameter of 5 mm; Bio-Rad Laboratories, Inc., Hercules, CA) at a concentration of 1.0 mg DNA/mg gold.

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA). ..

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany). .. RNA standards To obtain transcripts of equal length, we cut 5 mg of each plasmid with Spe I.

    Sequencing:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA). ..

    TA Cloning:

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: The PCR product was cloned into pCR † 2.1-TOPO from the TOPO TA Cloning kit (Invitrogen, Cergy Pontoise, France) according to the manufacturer's instructions. .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany).

    Purification:

    Article Title: Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus.
    Article Snippet: .. Plasmid purification was conducted using a Qiagen Plasmid Maxi Kit (Qiagen Inc., Valencia, CA) according to manufacturer's instructions. .. Preparation of cartridges for intradermal biolistic delivery of cytokine cDNA Plasmid pINA or pcPIL12 was precipitated onto the surface of gold particles (average diameter of 5 mm; Bio-Rad Laboratories, Inc., Hercules, CA) at a concentration of 1.0 mg DNA/mg gold.

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: The purified DNA was ligated into the pCR2.1-TOPO (Invitrogen, Calsbad, CA, USA) cloning vector and transformed into competent cells (TOP10) (Invitrogen). .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany). .. RNA standards To obtain transcripts of equal length, we cut 5 mg of each plasmid with Spe I.

    Real-time Polymerase Chain Reaction:

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: Another plasmid was generated using a DNA fragment derived from the human GAPDH gene with the GAPDH1 and GAPDH2 primers (as used for real-time PCR for GAPDH). .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany).

    Polymerase Chain Reaction:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: Cloning and DNA sequencing The PCR products amplified with each primer pair were purified using GENECLEAN Turbo kit (BIO101, La Jolla, CA, USA) according to the manufacturer's recommendation. .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: The PCR product was cloned into pCR † 2.1-TOPO from the TOPO TA Cloning kit (Invitrogen, Cergy Pontoise, France) according to the manufacturer's instructions. .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany).

    Generated:

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: Another plasmid was generated using a DNA fragment derived from the human GAPDH gene with the GAPDH1 and GAPDH2 primers (as used for real-time PCR for GAPDH). .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany).

    DNA Sequencing:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: Paragraph title: Cloning and DNA sequencing ... Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA). .. For each TGEV strain, two or three independent clones originated from different PCR products were sequenced because of the possibility of errors arising during RT, PCR, or the cloning procedure.

    Transformation Assay:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: The purified DNA was ligated into the pCR2.1-TOPO (Invitrogen, Calsbad, CA, USA) cloning vector and transformed into competent cells (TOP10) (Invitrogen). .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    Recombinant:

    Article Title: Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.
    Article Snippet: Cells carrying recombinant plasmid were selected on LB agar plates containing kanamycin and X-gal. .. Plasmid DNA for sequencing was prepared by Plasmid Maxi kit (Qiagen, Santa Clarita, CA, USA).

    Derivative Assay:

    Article Title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay.
    Article Snippet: Another plasmid was generated using a DNA fragment derived from the human GAPDH gene with the GAPDH1 and GAPDH2 primers (as used for real-time PCR for GAPDH). .. The plasmids were then purified with the Qiagen plasmid maxi kit (Qiagen GmbH, Germany).

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    Qiagen qiagen maxi prep kit
    <t>DNA</t> purification from agarose gel. Digested plasmid was fractionated in an agarose gel (A) and a specific band (arrow) cut from the gel. The gel was divided in two and DNA from half was purified by four <t>Qiagen</t> gel extraction columns (2 in B) and the DNA from the other half was purified by a single glass syringe filter (3 in B). The original digestion mixture before purification is included (1 in B). The purified bands were analyzed on a 0.8% TAE agarose gel.
    Qiagen Maxi Prep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen maxi prep kit/product/Qiagen
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    qiagen maxi prep kit - by Bioz Stars, 2020-03
    95/100 stars
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    DNA purification from agarose gel. Digested plasmid was fractionated in an agarose gel (A) and a specific band (arrow) cut from the gel. The gel was divided in two and DNA from half was purified by four Qiagen gel extraction columns (2 in B) and the DNA from the other half was purified by a single glass syringe filter (3 in B). The original digestion mixture before purification is included (1 in B). The purified bands were analyzed on a 0.8% TAE agarose gel.

    Journal: PLoS ONE

    Article Title: A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters

    doi: 10.1371/journal.pone.0007750

    Figure Lengend Snippet: DNA purification from agarose gel. Digested plasmid was fractionated in an agarose gel (A) and a specific band (arrow) cut from the gel. The gel was divided in two and DNA from half was purified by four Qiagen gel extraction columns (2 in B) and the DNA from the other half was purified by a single glass syringe filter (3 in B). The original digestion mixture before purification is included (1 in B). The purified bands were analyzed on a 0.8% TAE agarose gel.

    Article Snippet: Therefore, plasmid purified using glass syringe filters appears to be of the same quality as plasmid DNA purified using a Qiagen Maxi-prep kit.

    Techniques: DNA Purification, Agarose Gel Electrophoresis, Plasmid Preparation, Purification, Gel Extraction

    Identification of recombinant pEGFP-KDR-TK by restriction enzyme digestion ( Sal I/ Xho I) and electrophoresis. Lane 1: DNA markers (λDNA/ Hin dIII); Lane 2-5: pEGFP-KDR-TK was digested with Sal I/ Xho I.

    Journal:

    Article Title: Expression of thymidine kinase mediated by a novel non-viral delivery system under the control of vascular endothelial growth factor receptor 2 promoter selectively kills human umbilical vein endothelial cells

    doi: 10.3748/wjg.14.224

    Figure Lengend Snippet: Identification of recombinant pEGFP-KDR-TK by restriction enzyme digestion ( Sal I/ Xho I) and electrophoresis. Lane 1: DNA markers (λDNA/ Hin dIII); Lane 2-5: pEGFP-KDR-TK was digested with Sal I/ Xho I.

    Article Snippet: The plasmid EGFP-KDR-TK was propagated in E. coli strain DH5α and purified using a large-scale plasmid DNA purification kit (Plasmid Maxi Kit; Qiagen, Hilden, Germany).

    Techniques: Recombinant, Electrophoresis

    Real-time PCR analysis of viral DNA replication in transfected Sf-9 cells. Shown are the results of three independent DNA replication assays. For these analyses, total DNA was isolated from Sf-9 cells transfected with either the Ac 101 (panel A), 142 (panel B), or the 144 (panel C) knockout bacmid or a bacmid lacking the gp64 envelope fusion protein which served as the non-infectious control. At the designated time-point, total DNA was extracted and digested with the restriction enzyme Dpn I to eliminate input bacmid DNA, and analyzed by real-time PCR. The values displayed represent the averages from transfections performed in triplicate with error bars indicating standard deviations.

    Journal: Virology

    Article Title: Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame

    doi: 10.1016/j.virol.2007.05.003

    Figure Lengend Snippet: Real-time PCR analysis of viral DNA replication in transfected Sf-9 cells. Shown are the results of three independent DNA replication assays. For these analyses, total DNA was isolated from Sf-9 cells transfected with either the Ac 101 (panel A), 142 (panel B), or the 144 (panel C) knockout bacmid or a bacmid lacking the gp64 envelope fusion protein which served as the non-infectious control. At the designated time-point, total DNA was extracted and digested with the restriction enzyme Dpn I to eliminate input bacmid DNA, and analyzed by real-time PCR. The values displayed represent the averages from transfections performed in triplicate with error bars indicating standard deviations.

    Article Snippet: Bacmid DNA was purified from 0.5 L cultures using the plasmid Maxi plasmid DNA purification kit (Qiagen).

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Isolation, Knock-Out