plasmid isolation kit  (Qiagen)

 
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    Name:
    QIAGEN Plasmid Mini Kit
    Description:
    For purification of up to 10 mg transfection grade plasmid or cosmid DNA Kit contents Qiagen Plasmid Mini Kit 25 preps 3 to 10mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 80 min Time Run 20g Yield 1 Sample Run For Purification of up to 20g Transfection grade Plasmid or Cosmid DNA Ideal for Transfection Cloning Sequencing Capillary Sequencing PCR In vitro Transcription Includes 25 Qiagen to tip 20 Reagents Buffers Benefits Purity equivalent to 2 x CsCl gradient centrifugation High yields of plasmid DNA Cost effective preparations LyseBlue for optimum lysis and maximum DNA yie
    Catalog Number:
    12123
    Price:
    214
    Category:
    QIAGEN Plasmid Kits
    Buy from Supplier


    Structured Review

    Qiagen plasmid isolation kit
    QIAGEN Plasmid Mini Kit
    For purification of up to 10 mg transfection grade plasmid or cosmid DNA Kit contents Qiagen Plasmid Mini Kit 25 preps 3 to 10mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 80 min Time Run 20g Yield 1 Sample Run For Purification of up to 20g Transfection grade Plasmid or Cosmid DNA Ideal for Transfection Cloning Sequencing Capillary Sequencing PCR In vitro Transcription Includes 25 Qiagen to tip 20 Reagents Buffers Benefits Purity equivalent to 2 x CsCl gradient centrifugation High yields of plasmid DNA Cost effective preparations LyseBlue for optimum lysis and maximum DNA yie
    https://www.bioz.com/result/plasmid isolation kit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plasmid isolation kit - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Function of Native OmtA In Vivo and Expression and Distribution of This Protein in Colonies of Aspergillus parasiticus
    Article Snippet: .. The proper construction of pLW12 in clones expressing MBP-OmtA was confirmed by restriction enzyme analysis of purified plasmid DNA isolated by the Qiagen (Valencia, Calif.) miniprep plasmid kit. .. The size of the fusion protein was determined by small-scale expression studies.

    Multiplex Assay:

    Article Title: Comparative Gene Expression Profiling of Tobacco-Associated HPV-Positive versus Negative Oral Squamous Carcinoma Cell Lines
    Article Snippet: .. Multiplex real-time PCRDNA was extracted by the DNA mini kit (Qiagen, Hilden, Germany) and HPV genotype evaluated by Anyplex II HPV28 assay, according to manufacturer's recommendations (Seegene, Seoul, South Korea). .. Data recording and interpretation were automated with the Seegene viewer software.

    Amplification:

    Article Title: Inflammatory Genital Infections Mitigate a Severe Genetic Bottleneck in Heterosexual Transmission of Subtype A and C HIV-1
    Article Snippet: .. End-Point Dilution, Single Genome Amplification of HIV-1 env Genes For amplification of proviral HIV-1 env genes, genomic DNA was extracted from uncultured peripheral blood mononuclear cells (PBMC) using the Qiagen DNA Blood Mini Kit. .. For amplification of plasma HIV-1 env genes, RNA was purified from plasma samples using the Qiagen Viral Isolation Kit and cDNA prepared using the SuperScript III reverse transcriptase according to the manufacturer's instructions.

    Article Title: JAK2V617F Allele Burden Measurement in Peripheral Blood of Iranian Patients with Myeloproliferative Neoplasms and Effect of Hydroxyurea on JAK2V617F Allele Burden
    Article Snippet: .. The study was approved by our institutional review board and written informed consent was obtained from all patients. (Ethical code: ir.tums.horcsct.1394.103.7) JAK2 V617F screening by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) Genomic DNA was prepared from leukocytes using the DNA blood mini kit (Qiagen, Germany). .. Mutation analysis of the JAK2 V617F was initially performed using ARMS-PCR.

    Mutagenesis:

    Article Title: JAK2V617F Allele Burden Measurement in Peripheral Blood of Iranian Patients with Myeloproliferative Neoplasms and Effect of Hydroxyurea on JAK2V617F Allele Burden
    Article Snippet: .. The study was approved by our institutional review board and written informed consent was obtained from all patients. (Ethical code: ir.tums.horcsct.1394.103.7) JAK2 V617F screening by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) Genomic DNA was prepared from leukocytes using the DNA blood mini kit (Qiagen, Germany). .. Mutation analysis of the JAK2 V617F was initially performed using ARMS-PCR.

    Isolation:

    Article Title: Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type lamins
    Article Snippet: .. In brief, DNA was isolated from cells expressing Dam or Dam-LMNB1 using DNA Mini kit (QIAGEN), precipitated, and resuspended to 1 µg/µl. .. 2 µg of this genomic DNA was digested overnight with the restriction enzyme DpnI (R0176L; New England Biolabs, Inc.), which cuts at methylated GAme TC.

    Article Title: Aberrant ZNF423 impedes B cell differentiation and is linked to adverse outcome of ETV6-RUNX1 negative B precursor acute lymphoblastic leukemia
    Article Snippet: .. Genomic DNA was isolated from initial ALL samples and matched MNC from remission BM using the QIAGEN DNA Blood Mini kit following the manufacturer’s instructions. .. Sample preparation, hybridization, and staining were performed according to the manufacturer’s standard protocol for Affymetrix GenomeWide Human SNP 6.0 microarrays.

    Article Title: Function of Native OmtA In Vivo and Expression and Distribution of This Protein in Colonies of Aspergillus parasiticus
    Article Snippet: .. The proper construction of pLW12 in clones expressing MBP-OmtA was confirmed by restriction enzyme analysis of purified plasmid DNA isolated by the Qiagen (Valencia, Calif.) miniprep plasmid kit. .. The size of the fusion protein was determined by small-scale expression studies.

    DNA Methylation Assay:

    Article Title: DNA demethylation enhances myoblasts hypertrophy during the late phase of myogenesis activating the IGF-I pathway
    Article Snippet: .. Global DNA methylation assay The total genomic DNA was extracted from the cells (treated with GM, DM, GMAZA or DMAZA) using the Qiagen DNA Mini Kit (Qiagen Sciences, Maryland, MD) following the manufacturer’s instructions. ..

    Expressing:

    Article Title: Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type lamins
    Article Snippet: .. In brief, DNA was isolated from cells expressing Dam or Dam-LMNB1 using DNA Mini kit (QIAGEN), precipitated, and resuspended to 1 µg/µl. .. 2 µg of this genomic DNA was digested overnight with the restriction enzyme DpnI (R0176L; New England Biolabs, Inc.), which cuts at methylated GAme TC.

    Article Title: Function of Native OmtA In Vivo and Expression and Distribution of This Protein in Colonies of Aspergillus parasiticus
    Article Snippet: .. The proper construction of pLW12 in clones expressing MBP-OmtA was confirmed by restriction enzyme analysis of purified plasmid DNA isolated by the Qiagen (Valencia, Calif.) miniprep plasmid kit. .. The size of the fusion protein was determined by small-scale expression studies.

    Purification:

    Article Title: Function of Native OmtA In Vivo and Expression and Distribution of This Protein in Colonies of Aspergillus parasiticus
    Article Snippet: .. The proper construction of pLW12 in clones expressing MBP-OmtA was confirmed by restriction enzyme analysis of purified plasmid DNA isolated by the Qiagen (Valencia, Calif.) miniprep plasmid kit. .. The size of the fusion protein was determined by small-scale expression studies.

    Plasmid Preparation:

    Article Title: Function of Native OmtA In Vivo and Expression and Distribution of This Protein in Colonies of Aspergillus parasiticus
    Article Snippet: .. The proper construction of pLW12 in clones expressing MBP-OmtA was confirmed by restriction enzyme analysis of purified plasmid DNA isolated by the Qiagen (Valencia, Calif.) miniprep plasmid kit. .. The size of the fusion protein was determined by small-scale expression studies.

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  • 99
    Qiagen plasmid dna isolation kit
    Quantitation of 2-LTR circle Junction <t>DNA</t> by Real-time <t>PCR.</t> Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA
    Plasmid Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna isolation kit/product/Qiagen
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    plasmid dna isolation kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Qiagen spin miniprep plasmid isolation kit
    Quantitation of 2-LTR circle Junction <t>DNA</t> by Real-time <t>PCR.</t> Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA
    Spin Miniprep Plasmid Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spin miniprep plasmid isolation kit/product/Qiagen
    Average 99 stars, based on 2135 article reviews
    Price from $9.99 to $1999.99
    spin miniprep plasmid isolation kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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    Quantitation of 2-LTR circle Junction DNA by Real-time PCR. Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Analysis of 2-LTR circle junctions of Viral DNA in infected cells

    doi: 10.1007/978-1-59745-170-3_6

    Figure Lengend Snippet: Quantitation of 2-LTR circle Junction DNA by Real-time PCR. Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA

    Article Snippet: i) Primers specific for 2-LTR circle junctions: 2-LTR forword -- 5' GCC TGG AGC TCT GGC TAA 3' and 2-LTR Reverse – 5' GCC TTG TGT GGT AGA TCC A 3' ( ) for first round PCR and MH535 and MH536 (Section 2.4. i.) for nested 2nd Round PCR. ii) TA cloning kit (Invitrogen) iii) Plasmid DNA isolation kit (QIAGEN) iv) DNA alignment software BioEdit ( ) (BioEdit v7.0.7: ).

    Techniques: Quantitation Assay, Real-time Polymerase Chain Reaction, Infection, Isolation