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Omega Bio-tek plasmid dna
PCR re-identification electrophoretogram. (A) PCR analysis of triple <t>transgenic</t> K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp <t>DNA</t> ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
Plasmid Dna, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna/product/Omega Bio-tek
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
plasmid dna - by Bioz Stars, 2021-03
86/100 stars

Images

1) Product Images from "Genetic characteristics of polycistronic system-mediated randomly-inserted multi-transgenes in miniature pigs and mice"

Article Title: Genetic characteristics of polycistronic system-mediated randomly-inserted multi-transgenes in miniature pigs and mice

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2017.7842

PCR re-identification electrophoretogram. (A) PCR analysis of triple transgenic K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp DNA ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
Figure Legend Snippet: PCR re-identification electrophoretogram. (A) PCR analysis of triple transgenic K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp DNA ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.

Techniques Used: Polymerase Chain Reaction, Transgenic Assay, Mouse Assay, Marker, Positive Control, Plasmid Preparation, Negative Control, Dominant Negative Mutation

2) Product Images from "Protocell Arrays for Simultaneous Detection of Diverse Analytes"

Article Title: Protocell Arrays for Simultaneous Detection of Diverse Analytes

Journal: bioRxiv

doi: 10.1101/2021.02.13.431022

Multiplexed detection of clinically relevant biomarkers across molecular classes in water and 20% human serum matrices. ( A ) Schematic of membrane-less protocell array setup for multi-modal, multiplexed detection of diverse classes of clinically relevant biomarkers. Purple and yellow circles indicate micro-basins containing zinc and vitamin B 12 sensors, respectively. Red and blue circles indicate micro-basins containing Stx1 and Stx2 toehold switches, respectively. ( B ) Representative fluorescence image (top) and quantification of fluorescence of representative image and its replicates (bottom) demonstrating multi-modal target measurement multiplexing using protocell arrays, with simultaneous detection of ion, small molecule, RNA, and DNA targets from the same bulk phase. Error bars represent standard deviations of technical triplicates. ( C ) Multi-modal target detection in a 20% human serum matrix. Representative fluorescence image (top) and quantification of fluorescence of representative image and its replicates (bottom) demonstrating robustness of protocell arrays to a complex sample matrix. Error bars represent standard deviations of technical triplicates.
Figure Legend Snippet: Multiplexed detection of clinically relevant biomarkers across molecular classes in water and 20% human serum matrices. ( A ) Schematic of membrane-less protocell array setup for multi-modal, multiplexed detection of diverse classes of clinically relevant biomarkers. Purple and yellow circles indicate micro-basins containing zinc and vitamin B 12 sensors, respectively. Red and blue circles indicate micro-basins containing Stx1 and Stx2 toehold switches, respectively. ( B ) Representative fluorescence image (top) and quantification of fluorescence of representative image and its replicates (bottom) demonstrating multi-modal target measurement multiplexing using protocell arrays, with simultaneous detection of ion, small molecule, RNA, and DNA targets from the same bulk phase. Error bars represent standard deviations of technical triplicates. ( C ) Multi-modal target detection in a 20% human serum matrix. Representative fluorescence image (top) and quantification of fluorescence of representative image and its replicates (bottom) demonstrating robustness of protocell arrays to a complex sample matrix. Error bars represent standard deviations of technical triplicates.

Techniques Used: Fluorescence, Multiplexing

Schematic of the membrane-less protocell array-based, multiplexed, multiple analyte sensing platform. The use of spatially patterned arrays of phase-separated protocell sensors enables each microwell to provide measurements for multiple analytes using a single type of fluorescent or colorimetric readout. (A) An overhead view of a microwell with four micro-basins. White area surrounding the micro-basins (yellow-shaded regions) represents the bulk phase formed by a PEG solution containing the analytes to be detected. Each micro-basin has a protocell containing CFE reaction (represented by the microcentrifuge tube) with a different sensor plasmid or genetic circuit. (B) Side view of a microwell with micro-basins. Analytes in the bulk phase (blue diamonds) enter protocells to activate their cognate sensor (blue DNA construct), producing detectable reporter signals. Levels of multiple analytes can be simultaneously assessed by tracking which protocell produces reporter signals (green color on the right).
Figure Legend Snippet: Schematic of the membrane-less protocell array-based, multiplexed, multiple analyte sensing platform. The use of spatially patterned arrays of phase-separated protocell sensors enables each microwell to provide measurements for multiple analytes using a single type of fluorescent or colorimetric readout. (A) An overhead view of a microwell with four micro-basins. White area surrounding the micro-basins (yellow-shaded regions) represents the bulk phase formed by a PEG solution containing the analytes to be detected. Each micro-basin has a protocell containing CFE reaction (represented by the microcentrifuge tube) with a different sensor plasmid or genetic circuit. (B) Side view of a microwell with micro-basins. Analytes in the bulk phase (blue diamonds) enter protocells to activate their cognate sensor (blue DNA construct), producing detectable reporter signals. Levels of multiple analytes can be simultaneously assessed by tracking which protocell produces reporter signals (green color on the right).

Techniques Used: Plasmid Preparation, Construct

Multiplexed detection of model nucleic acid sequences in membrane-less protocell arrays. (A) Schematic of toehold switch mechanism. In the absence of trigger RNA, the switch RNA forms an inhibitory hairpin that blocks translation of a reporter (here, GFP). When trigger RNA is present, the switch RNA hybridizes with trigger RNA and unwinds the inhibitory hairpin, allowing translation of GFP. (B) Schematic of protocell array setup for multiplexed detection of nucleic acids. Red and blue circles indicate micro-basins containing toehold switches B and H, respectively. Gray circles indicate no-plasmid control protocells. (C) Representative fluorescence image of multiplexed RNA detection with minimal crosstalk. Images in the same column have the same RNA trigger(s) added. Images in the same row have the same concentration of trigger(s) added. Circle colors are as in (B). (D) Quantification of fluorescence images in (C) and their replicates. Error bars represent standard deviations of technical triplicates. (E) Representative fluorescence image of multiplexed detection of linear DNA with minimal crosstalk. Images in the same column have the same DNA trigger(s) added. Images in the same row have the same concentration of DNA trigger(s) added. Circle colors are as in (B). Although addition of both DNA triggers mutually represses their outputs, this was found to be specific to the use of these two triggers. (F) Quantification of fluorescence images in (E) and their replicates. Error bars represent standard deviations of technical triplicates.
Figure Legend Snippet: Multiplexed detection of model nucleic acid sequences in membrane-less protocell arrays. (A) Schematic of toehold switch mechanism. In the absence of trigger RNA, the switch RNA forms an inhibitory hairpin that blocks translation of a reporter (here, GFP). When trigger RNA is present, the switch RNA hybridizes with trigger RNA and unwinds the inhibitory hairpin, allowing translation of GFP. (B) Schematic of protocell array setup for multiplexed detection of nucleic acids. Red and blue circles indicate micro-basins containing toehold switches B and H, respectively. Gray circles indicate no-plasmid control protocells. (C) Representative fluorescence image of multiplexed RNA detection with minimal crosstalk. Images in the same column have the same RNA trigger(s) added. Images in the same row have the same concentration of trigger(s) added. Circle colors are as in (B). (D) Quantification of fluorescence images in (C) and their replicates. Error bars represent standard deviations of technical triplicates. (E) Representative fluorescence image of multiplexed detection of linear DNA with minimal crosstalk. Images in the same column have the same DNA trigger(s) added. Images in the same row have the same concentration of DNA trigger(s) added. Circle colors are as in (B). Although addition of both DNA triggers mutually represses their outputs, this was found to be specific to the use of these two triggers. (F) Quantification of fluorescence images in (E) and their replicates. Error bars represent standard deviations of technical triplicates.

Techniques Used: Plasmid Preparation, Fluorescence, RNA Detection, Concentration Assay

Related Articles

Plasmid Preparation:

Article Title: A point mutation in the polymerase protein PB2 allows a reassortant H9N2 influenza isolate of wild-bird origin to replicate in human cells
Article Snippet: The identity of all resulting recombinant plasmids was verified by restriction digestion and Sanger sequencing (Genewiz). .. Maxipreps of all plasmid DNAs were obtained using the Plasmid Maxi Kit (Omega Bio-Tek). .. To rescue wild-type and mutant viruses by reverse genetics, PCR-amplified full genomic segments were digested with LguI restriction enzyme (Thermo Scientific) and cloned into the ambisense pDZ vector (obtained from Adolfo Garcia-Sastre, Icahn School of Medicine at Mount Sinai) ( ).

Article Title: DNA repair of clustered lesions in mammalian cells: involvement of non-homologous end-joining
Article Snippet: Plasmid survival assay and analysis of re-isolated DNA Three different ligation reactions for each type of construct were nucleofected in duplicate into the cells with 100 ng pACYC184. .. After 6 h at 37°C and 5% CO2 , plasmid DNA was re-isolated using the E.Z.N.A Plasmid Mini Kit I (OMEGA Bio-Tek Inc., Doraville, GA, USA) and DNA from the duplicate transfections were pooled to form a sample. .. Each DNA sample was then transformed in duplicate into Max efficiency DH5αTM chemically competent cells (Invitrogen Corporation, Carlsbad, CA, USA) and allowed to grow for 1 h. Cultures were then plated in triplicate on solid medium containing either 100 μg/ml carbenicillin or 34 μg/ml chloramphenicol.

Article Title: Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature
Article Snippet: We used the following PCR mutagenesis conditions: one cycle at 95°C for 2 min, 18 cycles at 98°C for 30 sec followed by 50°C or 55°C annealing for 1 min and extension at 72°C for 5 min, plus final extension for 5 min. We treated PCR products with 20 U DpnI (New England Biolabs) to remove methylated PCR templates followed by DH5α transformation. .. After overnight growth with Lysogeny broth (LB) and 70 µg/mL ampicillin, we purified plasmid DNA by miniprep (Omega Bio-Tek) and confirmed all sequences by Sanger sequencing with specific primers (1st Base Asia). .. RNA extraction and RT-PCR We collected 2 × 106 cells for each condition, washed them with 1× PBS, and extracted total RNA using PureLink RNA Mini Kit (Life Technologies) following the kit's manual.

Article Title: Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis
Article Snippet: EcFtsZ mutants were constructed by site-directed mutagenesis using the Quick Change Mutagenesis Kit from Stratagene. .. All plasmid DNAs were purified with E.Z.N.A Plasmid Miniprep Kit I from Omega Bio-tek. .. Each ftsZ gene with the corresponding point mutation was completely sequenced.

Article Title: Identification of endonuclease domain-containing 1 as a novel tumor suppressor in prostate cancer
Article Snippet: .. Colonies were inoculated in the lysogeny broth medium containing with kanamycin 100 μg/ml overnight at 37 °C, 150 r/min and then plasmid DNA was extracted according to instructions of Endo-Free Plasmid Midi Kit (Omega biotech). ..

Article Title: Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus
Article Snippet: The recombinant plasmids, designated as pcDNA-2L and pcDNA-58L, was confirmed by using restriction enzyme digestion and DNA sequencing. .. Both plasmids (pcDNA-2L and pcDNA-58L) and the empty vector (pcDNA3.1) were purified from overnight cultures of transformed Escherichia coli strain DH5α cells with using the Endo-free Plasmid Midi Kit (Omega Bio-Tek, Norcross, GA, USA). .. Detection of Expression of Vaccine Plasmids in EPC Cells EPC cells were seeded into 6-well culture plates and grown in medium199 supplemented with 5% fetal bovine serum (FBS).

Article Title: Protocell Arrays for Simultaneous Detection of Diverse Analytes
Article Snippet: Assembled constructs were transformed into DH10β cells, and isolated colonies were grown overnight in LB with antibiotics. .. Plasmid DNA from overnight cultures was purified using EZNA miniprep columns (OMEGA Bio-Tek). .. Plasmid sequences were verified with Sanger DNA sequencing (Eurofins Genomics).

Article Title: PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells, et al. PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells
Article Snippet: The pCMV‐IκBαM vector was commercially purchased (631923, Takara), which contains a mutated form of IκBα with a serine‐to‐alanine mutation at residues 32 and 36 resulting in failure of signal‐induced phosphorylation. .. All plasmid DNAs were extracted and purified with EZNA Endo‐free Plasmid Midi Kit (D6915‐03, Omega Bio‐Tek) and sequenced (Invitrogen). .. 2.3 Transient transfection and Dual‐luciferase reporter assay The pGL3‐WT and PGL3‐Mutant luciferase reporter vectors were transfected alone or each cotransfected with pCMV‐p65 plasmid into HepG2 cells using X‐tremeGENE HP DNA Transfection Reagent (06366236001, Roche).

Transfection:

Article Title: DNA repair of clustered lesions in mammalian cells: involvement of non-homologous end-joining
Article Snippet: Plasmid survival assay and analysis of re-isolated DNA Three different ligation reactions for each type of construct were nucleofected in duplicate into the cells with 100 ng pACYC184. .. After 6 h at 37°C and 5% CO2 , plasmid DNA was re-isolated using the E.Z.N.A Plasmid Mini Kit I (OMEGA Bio-Tek Inc., Doraville, GA, USA) and DNA from the duplicate transfections were pooled to form a sample. .. Each DNA sample was then transformed in duplicate into Max efficiency DH5αTM chemically competent cells (Invitrogen Corporation, Carlsbad, CA, USA) and allowed to grow for 1 h. Cultures were then plated in triplicate on solid medium containing either 100 μg/ml carbenicillin or 34 μg/ml chloramphenicol.

Purification:

Article Title: Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature
Article Snippet: We used the following PCR mutagenesis conditions: one cycle at 95°C for 2 min, 18 cycles at 98°C for 30 sec followed by 50°C or 55°C annealing for 1 min and extension at 72°C for 5 min, plus final extension for 5 min. We treated PCR products with 20 U DpnI (New England Biolabs) to remove methylated PCR templates followed by DH5α transformation. .. After overnight growth with Lysogeny broth (LB) and 70 µg/mL ampicillin, we purified plasmid DNA by miniprep (Omega Bio-Tek) and confirmed all sequences by Sanger sequencing with specific primers (1st Base Asia). .. RNA extraction and RT-PCR We collected 2 × 106 cells for each condition, washed them with 1× PBS, and extracted total RNA using PureLink RNA Mini Kit (Life Technologies) following the kit's manual.

Article Title: Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis
Article Snippet: EcFtsZ mutants were constructed by site-directed mutagenesis using the Quick Change Mutagenesis Kit from Stratagene. .. All plasmid DNAs were purified with E.Z.N.A Plasmid Miniprep Kit I from Omega Bio-tek. .. Each ftsZ gene with the corresponding point mutation was completely sequenced.

Article Title: Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus
Article Snippet: The recombinant plasmids, designated as pcDNA-2L and pcDNA-58L, was confirmed by using restriction enzyme digestion and DNA sequencing. .. Both plasmids (pcDNA-2L and pcDNA-58L) and the empty vector (pcDNA3.1) were purified from overnight cultures of transformed Escherichia coli strain DH5α cells with using the Endo-free Plasmid Midi Kit (Omega Bio-Tek, Norcross, GA, USA). .. Detection of Expression of Vaccine Plasmids in EPC Cells EPC cells were seeded into 6-well culture plates and grown in medium199 supplemented with 5% fetal bovine serum (FBS).

Article Title: Protocell Arrays for Simultaneous Detection of Diverse Analytes
Article Snippet: Assembled constructs were transformed into DH10β cells, and isolated colonies were grown overnight in LB with antibiotics. .. Plasmid DNA from overnight cultures was purified using EZNA miniprep columns (OMEGA Bio-Tek). .. Plasmid sequences were verified with Sanger DNA sequencing (Eurofins Genomics).

Article Title: PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells, et al. PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells
Article Snippet: The pCMV‐IκBαM vector was commercially purchased (631923, Takara), which contains a mutated form of IκBα with a serine‐to‐alanine mutation at residues 32 and 36 resulting in failure of signal‐induced phosphorylation. .. All plasmid DNAs were extracted and purified with EZNA Endo‐free Plasmid Midi Kit (D6915‐03, Omega Bio‐Tek) and sequenced (Invitrogen). .. 2.3 Transient transfection and Dual‐luciferase reporter assay The pGL3‐WT and PGL3‐Mutant luciferase reporter vectors were transfected alone or each cotransfected with pCMV‐p65 plasmid into HepG2 cells using X‐tremeGENE HP DNA Transfection Reagent (06366236001, Roche).

Sequencing:

Article Title: Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature
Article Snippet: We used the following PCR mutagenesis conditions: one cycle at 95°C for 2 min, 18 cycles at 98°C for 30 sec followed by 50°C or 55°C annealing for 1 min and extension at 72°C for 5 min, plus final extension for 5 min. We treated PCR products with 20 U DpnI (New England Biolabs) to remove methylated PCR templates followed by DH5α transformation. .. After overnight growth with Lysogeny broth (LB) and 70 µg/mL ampicillin, we purified plasmid DNA by miniprep (Omega Bio-Tek) and confirmed all sequences by Sanger sequencing with specific primers (1st Base Asia). .. RNA extraction and RT-PCR We collected 2 × 106 cells for each condition, washed them with 1× PBS, and extracted total RNA using PureLink RNA Mini Kit (Life Technologies) following the kit's manual.

Transformation Assay:

Article Title: Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus
Article Snippet: The recombinant plasmids, designated as pcDNA-2L and pcDNA-58L, was confirmed by using restriction enzyme digestion and DNA sequencing. .. Both plasmids (pcDNA-2L and pcDNA-58L) and the empty vector (pcDNA3.1) were purified from overnight cultures of transformed Escherichia coli strain DH5α cells with using the Endo-free Plasmid Midi Kit (Omega Bio-Tek, Norcross, GA, USA). .. Detection of Expression of Vaccine Plasmids in EPC Cells EPC cells were seeded into 6-well culture plates and grown in medium199 supplemented with 5% fetal bovine serum (FBS).

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    Omega Bio-tek plasmid dna
    PCR re-identification electrophoretogram. (A) PCR analysis of triple <t>transgenic</t> K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp <t>DNA</t> ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
    Plasmid Dna, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna/product/Omega Bio-tek
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid dna - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Omega Bio-tek e z n a endo free plasmid dna maxi kit
    PCR re-identification electrophoretogram. (A) PCR analysis of triple <t>transgenic</t> K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp <t>DNA</t> ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
    E Z N A Endo Free Plasmid Dna Maxi Kit, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e z n a endo free plasmid dna maxi kit/product/Omega Bio-tek
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e z n a endo free plasmid dna maxi kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Omega Bio-tek omega ezna plasmid dna mini kit i
    PCR re-identification electrophoretogram. (A) PCR analysis of triple <t>transgenic</t> K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp <t>DNA</t> ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
    Omega Ezna Plasmid Dna Mini Kit I, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/omega ezna plasmid dna mini kit i/product/Omega Bio-tek
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    omega ezna plasmid dna mini kit i - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Omega Bio-tek e z n a plasmid dna mini kit ii
    PCR re-identification electrophoretogram. (A) PCR analysis of triple <t>transgenic</t> K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp <t>DNA</t> ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
    E Z N A Plasmid Dna Mini Kit Ii, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e z n a plasmid dna mini kit ii/product/Omega Bio-tek
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e z n a plasmid dna mini kit ii - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    PCR re-identification electrophoretogram. (A) PCR analysis of triple transgenic K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp DNA ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Genetic characteristics of polycistronic system-mediated randomly-inserted multi-transgenes in miniature pigs and mice

    doi: 10.3892/mmr.2017.7842

    Figure Lengend Snippet: PCR re-identification electrophoretogram. (A) PCR analysis of triple transgenic K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp DNA ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.

    Article Snippet: Development of gradient copy standard curves and determination of copy numbers Escherichia coli DH5α competent cells (Takara Biotechnology Co., Ltd.) containing transgenic vector plasmids were incubated overnight on a shaker, and the plasmid DNA was extracted using an Endo-free Plasmid Mini kit (E.Z.N.A; Omega Bio-Tek, Inc., Norcross, GA, USA).

    Techniques: Polymerase Chain Reaction, Transgenic Assay, Mouse Assay, Marker, Positive Control, Plasmid Preparation, Negative Control, Dominant Negative Mutation