plasmid dna (Omega Bio-tek)
86
Structured Review
Omega Bio-tek
plasmid dna
Plasmid Dna, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna/product/Omega Bio-tek
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Plasmid Dna, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna/product/Omega Bio-tek
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
plasmid dna - by Bioz Stars,
2021-03
86/100 stars
Images
1) Product Images from "Genetic characteristics of polycistronic system-mediated randomly-inserted multi-transgenes in miniature pigs and mice"
Article Title: Genetic characteristics of polycistronic system-mediated randomly-inserted multi-transgenes in miniature pigs and mice
Journal: Molecular Medicine Reports
doi: 10.3892/mmr.2017.7842
Figure Legend Snippet: PCR re-identification electrophoretogram. (A) PCR analysis of triple transgenic K3 mice. Three lanes/individual, with the following main fragment order: 11β-HSD1, CHOP, and hIAPP. A total of three simultaneous bands was regarded as a positive result. (B) PCR analysis of triple transgenic L3 mice. Three exogenous main gene fragments were detected individually. (C) PCR analysis of dual transgenic Z2 miniature pigs. Four simultaneous bands were regarded as positive. The primers are listed in Table I . Marker, 100 bp DNA ladder. M, marker; V, positive control plasmid vector; W, negative control wild-type mice; 11β-HSD1, 11β-hydroxysteroid dehydrogenase-1; CHOP, C/EBP homologous protein; hIAPP, human islet amyloid polypeptide; GIPR dn , dominant-negative gastric inhibitory polypeptide receptor; PIP, porcine insulin promoter; PCR, polymerase chain reaction.
Techniques Used: Polymerase Chain Reaction, Transgenic Assay, Mouse Assay, Marker, Positive Control, Plasmid Preparation, Negative Control, Dominant Negative Mutation
2) Product Images from "Protocell Arrays for Simultaneous Detection of Diverse Analytes"
Article Title: Protocell Arrays for Simultaneous Detection of Diverse Analytes
Journal: bioRxiv
doi: 10.1101/2021.02.13.431022
Figure Legend Snippet: Multiplexed detection of clinically relevant biomarkers across molecular classes in water and 20% human serum matrices. ( A ) Schematic of membrane-less protocell array setup for multi-modal, multiplexed detection of diverse classes of clinically relevant biomarkers. Purple and yellow circles indicate micro-basins containing zinc and vitamin B 12 sensors, respectively. Red and blue circles indicate micro-basins containing Stx1 and Stx2 toehold switches, respectively. ( B ) Representative fluorescence image (top) and quantification of fluorescence of representative image and its replicates (bottom) demonstrating multi-modal target measurement multiplexing using protocell arrays, with simultaneous detection of ion, small molecule, RNA, and DNA targets from the same bulk phase. Error bars represent standard deviations of technical triplicates. ( C ) Multi-modal target detection in a 20% human serum matrix. Representative fluorescence image (top) and quantification of fluorescence of representative image and its replicates (bottom) demonstrating robustness of protocell arrays to a complex sample matrix. Error bars represent standard deviations of technical triplicates.
Techniques Used: Fluorescence, Multiplexing
Figure Legend Snippet: Schematic of the membrane-less protocell array-based, multiplexed, multiple analyte sensing platform. The use of spatially patterned arrays of phase-separated protocell sensors enables each microwell to provide measurements for multiple analytes using a single type of fluorescent or colorimetric readout. (A) An overhead view of a microwell with four micro-basins. White area surrounding the micro-basins (yellow-shaded regions) represents the bulk phase formed by a PEG solution containing the analytes to be detected. Each micro-basin has a protocell containing CFE reaction (represented by the microcentrifuge tube) with a different sensor plasmid or genetic circuit. (B) Side view of a microwell with micro-basins. Analytes in the bulk phase (blue diamonds) enter protocells to activate their cognate sensor (blue DNA construct), producing detectable reporter signals. Levels of multiple analytes can be simultaneously assessed by tracking which protocell produces reporter signals (green color on the right).
Techniques Used: Plasmid Preparation, Construct
Figure Legend Snippet: Multiplexed detection of model nucleic acid sequences in membrane-less protocell arrays. (A) Schematic of toehold switch mechanism. In the absence of trigger RNA, the switch RNA forms an inhibitory hairpin that blocks translation of a reporter (here, GFP). When trigger RNA is present, the switch RNA hybridizes with trigger RNA and unwinds the inhibitory hairpin, allowing translation of GFP. (B) Schematic of protocell array setup for multiplexed detection of nucleic acids. Red and blue circles indicate micro-basins containing toehold switches B and H, respectively. Gray circles indicate no-plasmid control protocells. (C) Representative fluorescence image of multiplexed RNA detection with minimal crosstalk. Images in the same column have the same RNA trigger(s) added. Images in the same row have the same concentration of trigger(s) added. Circle colors are as in (B). (D) Quantification of fluorescence images in (C) and their replicates. Error bars represent standard deviations of technical triplicates. (E) Representative fluorescence image of multiplexed detection of linear DNA with minimal crosstalk. Images in the same column have the same DNA trigger(s) added. Images in the same row have the same concentration of DNA trigger(s) added. Circle colors are as in (B). Although addition of both DNA triggers mutually represses their outputs, this was found to be specific to the use of these two triggers. (F) Quantification of fluorescence images in (E) and their replicates. Error bars represent standard deviations of technical triplicates.
Techniques Used: Plasmid Preparation, Fluorescence, RNA Detection, Concentration Assay
Related Articles
Plasmid Preparation:Article Title: A point mutation in the polymerase protein PB2 allows a reassortant H9N2 influenza isolate of wild-bird origin to replicate in human cells Article Snippet: The identity of all resulting recombinant plasmids was verified by restriction digestion and Sanger sequencing (Genewiz). .. Maxipreps of all Article Title: DNA repair of clustered lesions in mammalian cells: involvement of non-homologous end-joining Article Snippet: Plasmid survival assay and analysis of re-isolated DNA Three different ligation reactions for each type of construct were nucleofected in duplicate into the cells with 100 ng pACYC184. .. After 6 h at 37°C and 5% CO2 , plasmid DNA was re-isolated using the Article Title: Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature Article Snippet: We used the following PCR mutagenesis conditions: one cycle at 95°C for 2 min, 18 cycles at 98°C for 30 sec followed by 50°C or 55°C annealing for 1 min and extension at 72°C for 5 min, plus final extension for 5 min. We treated PCR products with 20 U DpnI (New England Biolabs) to remove methylated PCR templates followed by DH5α transformation. .. After overnight growth with Lysogeny broth (LB) and 70 µg/mL ampicillin, we purified plasmid DNA by Article Title: Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis Article Snippet: EcFtsZ mutants were constructed by site-directed mutagenesis using the Quick Change Mutagenesis Kit from Stratagene. .. All Article Title: Identification of endonuclease domain-containing 1 as a novel tumor suppressor in prostate cancer Article Snippet: .. Colonies were inoculated in the lysogeny broth medium containing with kanamycin 100 μg/ml overnight at 37 °C, 150 r/min and then plasmid DNA was extracted according to instructions of Article Title: Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus Article Snippet: The recombinant plasmids, designated as pcDNA-2L and pcDNA-58L, was confirmed by using restriction enzyme digestion and DNA sequencing. .. Both Article Title: Protocell Arrays for Simultaneous Detection of Diverse Analytes Article Snippet: Assembled constructs were transformed into DH10β cells, and isolated colonies were grown overnight in LB with antibiotics. .. Plasmid DNA from overnight cultures was purified using Article Title: PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells, et al. PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells Article Snippet: The pCMV‐IκBαM vector was commercially purchased (631923, Takara), which contains a mutated form of IκBα with a serine‐to‐alanine mutation at residues 32 and 36 resulting in failure of signal‐induced phosphorylation. .. All Transfection:Article Title: DNA repair of clustered lesions in mammalian cells: involvement of non-homologous end-joining Article Snippet: Plasmid survival assay and analysis of re-isolated DNA Three different ligation reactions for each type of construct were nucleofected in duplicate into the cells with 100 ng pACYC184. .. After 6 h at 37°C and 5% CO2 , plasmid DNA was re-isolated using the Purification:Article Title: Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature Article Snippet: We used the following PCR mutagenesis conditions: one cycle at 95°C for 2 min, 18 cycles at 98°C for 30 sec followed by 50°C or 55°C annealing for 1 min and extension at 72°C for 5 min, plus final extension for 5 min. We treated PCR products with 20 U DpnI (New England Biolabs) to remove methylated PCR templates followed by DH5α transformation. .. After overnight growth with Lysogeny broth (LB) and 70 µg/mL ampicillin, we purified plasmid DNA by Article Title: Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis Article Snippet: EcFtsZ mutants were constructed by site-directed mutagenesis using the Quick Change Mutagenesis Kit from Stratagene. .. All Article Title: Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus Article Snippet: The recombinant plasmids, designated as pcDNA-2L and pcDNA-58L, was confirmed by using restriction enzyme digestion and DNA sequencing. .. Both Article Title: Protocell Arrays for Simultaneous Detection of Diverse Analytes Article Snippet: Assembled constructs were transformed into DH10β cells, and isolated colonies were grown overnight in LB with antibiotics. .. Plasmid DNA from overnight cultures was purified using Article Title: PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells, et al. PNPLA3 I148M mediates the regulatory effect of NF‐kB on inflammation in PA‐treated HepG2 cells Article Snippet: The pCMV‐IκBαM vector was commercially purchased (631923, Takara), which contains a mutated form of IκBα with a serine‐to‐alanine mutation at residues 32 and 36 resulting in failure of signal‐induced phosphorylation. .. All Sequencing:Article Title: Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature Article Snippet: We used the following PCR mutagenesis conditions: one cycle at 95°C for 2 min, 18 cycles at 98°C for 30 sec followed by 50°C or 55°C annealing for 1 min and extension at 72°C for 5 min, plus final extension for 5 min. We treated PCR products with 20 U DpnI (New England Biolabs) to remove methylated PCR templates followed by DH5α transformation. .. After overnight growth with Lysogeny broth (LB) and 70 µg/mL ampicillin, we purified plasmid DNA by Transformation Assay:Article Title: Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus Article Snippet: The recombinant plasmids, designated as pcDNA-2L and pcDNA-58L, was confirmed by using restriction enzyme digestion and DNA sequencing. .. Both |