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Axygen plasmid dna
Plasmid Dna, supplied by Axygen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna/product/Axygen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
plasmid dna - by Bioz Stars, 2021-03
86/100 stars

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Plasmid Preparation:

Article Title: Histone modifications interact with DNA methylation at the GATA4 promoter during differentiation of mesenchymal stem cells into cardiomyocyte‐like cells
Article Snippet: The PCR products were gel extracted (AP‐GX‐250; Axygen) and ligated into a plasmid vector using the pMD® 18‐T Vector (D101B; Takara). .. Plasmid‐transformed DH5α bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). .. At least 10 separate clones were chosen for sequence analysis.

Article Title: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
Article Snippet: The PCR products were extracted from the gel (AP-GX-250, Axygen) and ligated into the pMD®18-T Vector (D101B, TAKARA). .. Plasmid-transformed DH5α bacteria were cultured overnight, and plasmid DNA was isolated (Axygen). .. At least 10 separate clones were chosen for sequence analysis.

Article Title: Homocysteine activates autophagy by inhibition of CFTR expression via interaction between DNA methylation and H3K27me3 in mouse liver
Article Snippet: The PCR products were gel extracted (AP-GX-250; Axygen) and ligated into a plasmid vector using the pMD18-T Vector (D101B; Takara). .. Plasmid transformed DH5a bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). ..

Article Title: Emergence of Escherichia coli Sequence Type 131 Isolates Producing KPC-2 Carbapenemase in China
Article Snippet: .. Plasmid DNAs of E. coli transconjugants and transformants were obtained using the AxyPrep Plasmid Miniprep kit (Axygen Scientific, California) and were separated by electrophoresis. .. Plasmid DNA from E. coli isolates and E. coli transconjugants and transformants were used as templates in PCR amplification.

Article Title: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing.
Article Snippet: Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. .. Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. ..

Article Title: Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri
Article Snippet: After transformation of the ligation products into Escherichia coli trans 5α chemically competent cells, positive colonies were identified among cultured single colonies grown at 37°C overnight in LB medium. .. An AxyPrep™ 96 Plasmid Kit (Axygen,) was used to extract plasmid DNA from a positive colony cultured in LB liquid medium at 37°C for 12–16 h. The concentration of plasmid DNA was measured using a spectrometer (Beckman, USA) and recalculated to obtain a value for plasmid copies/μL. .. The cloned plasmid DNA was linearized by endogenous restriction enzymes Hind III (FastDigest; ThermoFisher, USA) which does not target any sequence in the PCR amplicon.

Article Title: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
Article Snippet: After washing the protoplasts two times with W5 washing buffer, cell counts of protoplasts was done using a hemacytometer under a microscope and the final protoplast concentration was adjust to 2 × 106 per milliliter. .. For protoplast transformation, 30 μL plasmid DNA (1 μg/ μL; prepared by Axygen midiprep kit) was used for transformation of 200 μL protoplasts by gently mixing with 230 μL 40% PEG transformation buffer. ..

Cell Culture:

Article Title: Histone modifications interact with DNA methylation at the GATA4 promoter during differentiation of mesenchymal stem cells into cardiomyocyte‐like cells
Article Snippet: The PCR products were gel extracted (AP‐GX‐250; Axygen) and ligated into a plasmid vector using the pMD® 18‐T Vector (D101B; Takara). .. Plasmid‐transformed DH5α bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). .. At least 10 separate clones were chosen for sequence analysis.

Article Title: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
Article Snippet: The PCR products were extracted from the gel (AP-GX-250, Axygen) and ligated into the pMD®18-T Vector (D101B, TAKARA). .. Plasmid-transformed DH5α bacteria were cultured overnight, and plasmid DNA was isolated (Axygen). .. At least 10 separate clones were chosen for sequence analysis.

Article Title: Homocysteine activates autophagy by inhibition of CFTR expression via interaction between DNA methylation and H3K27me3 in mouse liver
Article Snippet: The PCR products were gel extracted (AP-GX-250; Axygen) and ligated into a plasmid vector using the pMD18-T Vector (D101B; Takara). .. Plasmid transformed DH5a bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). ..

Article Title: Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri
Article Snippet: After transformation of the ligation products into Escherichia coli trans 5α chemically competent cells, positive colonies were identified among cultured single colonies grown at 37°C overnight in LB medium. .. An AxyPrep™ 96 Plasmid Kit (Axygen,) was used to extract plasmid DNA from a positive colony cultured in LB liquid medium at 37°C for 12–16 h. The concentration of plasmid DNA was measured using a spectrometer (Beckman, USA) and recalculated to obtain a value for plasmid copies/μL. .. The cloned plasmid DNA was linearized by endogenous restriction enzymes Hind III (FastDigest; ThermoFisher, USA) which does not target any sequence in the PCR amplicon.

Isolation:

Article Title: Histone modifications interact with DNA methylation at the GATA4 promoter during differentiation of mesenchymal stem cells into cardiomyocyte‐like cells
Article Snippet: The PCR products were gel extracted (AP‐GX‐250; Axygen) and ligated into a plasmid vector using the pMD® 18‐T Vector (D101B; Takara). .. Plasmid‐transformed DH5α bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). .. At least 10 separate clones were chosen for sequence analysis.

Article Title: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
Article Snippet: The PCR products were extracted from the gel (AP-GX-250, Axygen) and ligated into the pMD®18-T Vector (D101B, TAKARA). .. Plasmid-transformed DH5α bacteria were cultured overnight, and plasmid DNA was isolated (Axygen). .. At least 10 separate clones were chosen for sequence analysis.

Article Title: Homocysteine activates autophagy by inhibition of CFTR expression via interaction between DNA methylation and H3K27me3 in mouse liver
Article Snippet: The PCR products were gel extracted (AP-GX-250; Axygen) and ligated into a plasmid vector using the pMD18-T Vector (D101B; Takara). .. Plasmid transformed DH5a bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). ..

Transformation Assay:

Article Title: Homocysteine activates autophagy by inhibition of CFTR expression via interaction between DNA methylation and H3K27me3 in mouse liver
Article Snippet: The PCR products were gel extracted (AP-GX-250; Axygen) and ligated into a plasmid vector using the pMD18-T Vector (D101B; Takara). .. Plasmid transformed DH5a bacteria were cultured overnight, and the plasmid DNA was isolated (Axygen). ..

Article Title: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing.
Article Snippet: Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. .. Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. ..

Article Title: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
Article Snippet: After washing the protoplasts two times with W5 washing buffer, cell counts of protoplasts was done using a hemacytometer under a microscope and the final protoplast concentration was adjust to 2 × 106 per milliliter. .. For protoplast transformation, 30 μL plasmid DNA (1 μg/ μL; prepared by Axygen midiprep kit) was used for transformation of 200 μL protoplasts by gently mixing with 230 μL 40% PEG transformation buffer. ..

Electrophoresis:

Article Title: Emergence of Escherichia coli Sequence Type 131 Isolates Producing KPC-2 Carbapenemase in China
Article Snippet: .. Plasmid DNAs of E. coli transconjugants and transformants were obtained using the AxyPrep Plasmid Miniprep kit (Axygen Scientific, California) and were separated by electrophoresis. .. Plasmid DNA from E. coli isolates and E. coli transconjugants and transformants were used as templates in PCR amplification.

Concentration Assay:

Article Title: Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri
Article Snippet: After transformation of the ligation products into Escherichia coli trans 5α chemically competent cells, positive colonies were identified among cultured single colonies grown at 37°C overnight in LB medium. .. An AxyPrep™ 96 Plasmid Kit (Axygen,) was used to extract plasmid DNA from a positive colony cultured in LB liquid medium at 37°C for 12–16 h. The concentration of plasmid DNA was measured using a spectrometer (Beckman, USA) and recalculated to obtain a value for plasmid copies/μL. .. The cloned plasmid DNA was linearized by endogenous restriction enzymes Hind III (FastDigest; ThermoFisher, USA) which does not target any sequence in the PCR amplicon.

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  • 86
    Axygen plasmid dna
    <t>PCR</t> product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic <t>DNA</t> as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.
    Plasmid Dna, supplied by Axygen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna/product/Axygen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid dna - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Axygen plasmid dna preparation kit
    <t>PCR</t> product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic <t>DNA</t> as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.
    Plasmid Dna Preparation Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna preparation kit/product/Axygen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid dna preparation kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    PCR product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic DNA as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.

    Journal: Scientific Reports

    Article Title: Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus

    doi: 10.1038/srep11997

    Figure Lengend Snippet: PCR product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic DNA as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.

    Article Snippet: The plasmid DNA containing the PCR fragment insert was isolated from each culture using AxyPrep Plasmid Miniprep kit (Axygen, USA) according to manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis