plasmid description reference strains f nucleatum 23726 parental strain  (ATCC)


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    ATCC plasmid description reference strains f nucleatum 23726 parental strain
    Bacterial strains and plasmids used
    Plasmid Description Reference Strains F Nucleatum 23726 Parental Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Use of CRISPR interference for efficient and rapid gene inactivation in Fusobacterium nucleatum"

    Article Title: Use of CRISPR interference for efficient and rapid gene inactivation in Fusobacterium nucleatum

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/aem.01665-23

    Bacterial strains and plasmids used
    Figure Legend Snippet: Bacterial strains and plasmids used

    Techniques Used: Plasmid Preparation, Isolation, Clone Assay, Expressing

    plasmid description reference strains f nucleatum 23726 parental strain  (ATCC)


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    ATCC plasmid description reference strains f nucleatum 23726 parental strain
    Bacterial strains and plasmids used
    Plasmid Description Reference Strains F Nucleatum 23726 Parental Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid description reference strains f nucleatum 23726 parental strain/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid description reference strains f nucleatum 23726 parental strain - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Use of CRISPR interference for efficient and rapid gene inactivation in Fusobacterium nucleatum"

    Article Title: Use of CRISPR interference for efficient and rapid gene inactivation in Fusobacterium nucleatum

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/aem.01665-23

    Bacterial strains and plasmids used
    Figure Legend Snippet: Bacterial strains and plasmids used

    Techniques Used: Plasmid Preparation, Isolation, Clone Assay, Expressing

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    ATCC plasmid description reference strains f nucleatum 23726 parental strain
    Bacterial strains and plasmids used
    Plasmid Description Reference Strains F Nucleatum 23726 Parental Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid description reference strains f nucleatum 23726 parental strain/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid description reference strains f nucleatum 23726 parental strain - by Bioz Stars, 2024-04
    86/100 stars
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    Bacterial strains and plasmids used

    Journal: Applied and Environmental Microbiology

    Article Title: Use of CRISPR interference for efficient and rapid gene inactivation in Fusobacterium nucleatum

    doi: 10.1128/aem.01665-23

    Figure Lengend Snippet: Bacterial strains and plasmids used

    Article Snippet: TABLE 2 Strain and plasmid Description Reference Strains F. nucleatum 23726 Parental strain (wild-type strain) From ATCC F. nucleatum CTI-2 Wild-type strain, isolated from human CRC samples ( 27 ) F. periodonticum 33693 Wild-type strain From ATCC F. nucleatum CW2 ∆ ftsX ; an isogenic derivative of CW1 ( 25 ) F. nucleatum BG01 ∆ radD ; an isogenic derivative of 23726 ( 39 ) F. nucleatum ZP05 WT strain 23726 containing pZP4C This study F. nucleatum ZP06 WT strain 23726 containing pZP4C( radD ) This study F. nucleatum ZP07 WT strain 23726 containing pZP4C( bamA ) This study F. nucleatum ZP07a WT strain 23726 containing pZP4C( control ) This study F. nucleatum ZP07b WT strain 23726 containing pZP4C( bamA )-P- bamA′ This study F. nucleatum ZP07c WT strain 23726 containing pZP4C( bamA )-P- skp-lpxD-glpQ This study F. nucleatum ZP07d WT strain 23726 containing pZP4C( bamA )-P- bamA′-skp-lpxD-glpQ This study F. nucleatum ZP08 WT strain 23726 containing pZP4C( ftsZ ) This study F. nucleatum ZP09 WT strain 33693 containing pZP4C( clpB Fp ) This study F. nucleatum ZP10 WT strain 33693 containing pZP4C( tnaA Fp ) This study F. nucleatum ZP11 WT strain CTI-2 containing pZP4C( tnaA CTI-2 ) This study E. coli DH5α Cloning host From NEB Plasmids pCWU6 E. coli / Fusobacterium shuttle vector, chloramphenicol /thiamphenicol resistance; cm R /thia R ( 25 ) pRECas1 The fdx promoter incorporating the riboswitch element E controls Cas9 expression ( 44 ) pIA33 P xyl::dcas9-opt P gdh ::sgRNA- rfp catP ( 67 ) pZP4C P fdx ::dcas9-opt P rpsJ FP ::sgRNA- ftsX catP This study pZP4C( radD ) P fdx ::dcas9-opt P rpsJ FP ::sgRNA- radD catP This study pZP4C- mCherry P fdx ::dcas9-opt P rpsJ FP ::P rpsJ cd :: mCherry catP This study pZP4C(control) P fdx ::dcas9-opt catP This study pZP4C( bamA ) P fdx ::dcas9-opt P rpsJ FP ::sgRNA- bamA catP This study pZP4C( bamA )-P- bamA ′ P fdx ::dcas9-opt P rpsJ FP ::sgRNA- bamA P bamA-bamA’ catP This study pZP4C( bamA )-P- skp-lpxD-glpQ P fdx ::dcas9-opt P rpsJ FP ::sgRNA- bamA P bamA-skp-lpxD-glpQ catP This study pZP4C( bamA )-P- bamA′-skp-lpxD-glpQ P fdx ::dcas9-opt P rpsJ FP ::sgRNA- bamA P bamA-bamA-skp-lpxD-glpQ catP This study pZP4C( ftsZ ) P fdx ::dcas9-opt P rpsJ FP ::sgRNA- ftsZ catP This study pZP4C( clpB Fp ) P fdx ::dcas9-opt P rpsJFP ::sgRNA- clpBFP catP This study pZP4C( tnaA Fp ) P fdx ::dcas9-opt P rpsJ FP ::sgRNA- tnaA FP catP This study pZP4C( tnaA CTI-2 ) P fdx ::dcas9-opt P rpsJ FP ::sgRNA- tnaA CTI-2 catP This study Open in a separate window Bacterial strains and plasmids used. . Plasmid and strain construction All constructed plasmids in this study are listed in .

    Techniques: Plasmid Preparation, Isolation, Clone Assay, Expressing