plasma hbv dna  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Roche plasma hbv dna
    Comparison of <t>HBV</t> replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV <t>DNA</t> from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Plasma Hbv Dna, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma hbv dna/product/Roche
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    plasma hbv dna - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients"

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    Journal: Viruses

    doi: 10.3390/v11010078

    Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Figure Legend Snippet: Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Techniques Used: Mutagenesis, Transfection, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).
    Figure Legend Snippet: Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Techniques Used: Mutagenesis, MANN-WHITNEY

    2) Product Images from "High rates of chronic HBV genotype E infection in a group of migrants in Italy from West Africa: Virological characteristics associated with poor immune clearance"

    Article Title: High rates of chronic HBV genotype E infection in a group of migrants in Italy from West Africa: Virological characteristics associated with poor immune clearance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0195045

    Graphs reporting the correlation between cccDNA and HBsAg titer (A) and serum HBV-DNA (B), and between intrahepatic total HBV-DNA and HBsAg titer (c) and serum HBV-DNA (D) in the 5 HBV genotype E infected patients with available liver biopsies.
    Figure Legend Snippet: Graphs reporting the correlation between cccDNA and HBsAg titer (A) and serum HBV-DNA (B), and between intrahepatic total HBV-DNA and HBsAg titer (c) and serum HBV-DNA (D) in the 5 HBV genotype E infected patients with available liver biopsies.

    Techniques Used: Infection

    3) Product Images from "Detection of Hepatitis B Virus (HBV) Genomes and HBV Drug Resistant Variants by Deep Sequencing Analysis of HBV Genomes in Immune Cell Subsets of HBV Mono-Infected and/or Human Immunodeficiency Virus Type-1 (HIV-1) and HBV Co-Infected Individuals"

    Article Title: Detection of Hepatitis B Virus (HBV) Genomes and HBV Drug Resistant Variants by Deep Sequencing Analysis of HBV Genomes in Immune Cell Subsets of HBV Mono-Infected and/or Human Immunodeficiency Virus Type-1 (HIV-1) and HBV Co-Infected Individuals

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0137568

    Comparison of median HBV covalently closed circular (ccc) DNA copies in peripheral blood mononuclear cells in HBV mono-infected (before and after antiviral treatment) and HBV/HIV-1 co-infected patients. The HBV covalently closed circular (ccc)—DNA copies/peripheral blood mononuclear cell (PBMC) were determined by quantitative PCR using a TaqMan probe and normalized to a housekeeping gene (i.e., β-globin, β-glo). The HBV cccDNA copies/PBMC did not significantly differ between groups, i.e, HBV treatment naïve mono-infected group (n = 11): median 4.2, range 3.4–4.7 log10 copies/10 5 PBMC; HBV mono-infected on antiviral therapy (n = 4): median 3.8, range 3.6–3.9 log10 copies/10 5 PBMC; and HBV/HIV-1 co-infected (n = 2) mean 3.8 copies/10 5 PBMC.
    Figure Legend Snippet: Comparison of median HBV covalently closed circular (ccc) DNA copies in peripheral blood mononuclear cells in HBV mono-infected (before and after antiviral treatment) and HBV/HIV-1 co-infected patients. The HBV covalently closed circular (ccc)—DNA copies/peripheral blood mononuclear cell (PBMC) were determined by quantitative PCR using a TaqMan probe and normalized to a housekeeping gene (i.e., β-globin, β-glo). The HBV cccDNA copies/PBMC did not significantly differ between groups, i.e, HBV treatment naïve mono-infected group (n = 11): median 4.2, range 3.4–4.7 log10 copies/10 5 PBMC; HBV mono-infected on antiviral therapy (n = 4): median 3.8, range 3.6–3.9 log10 copies/10 5 PBMC; and HBV/HIV-1 co-infected (n = 2) mean 3.8 copies/10 5 PBMC.

    Techniques Used: Countercurrent Chromatography, Infection, Real-time Polymerase Chain Reaction

    4) Product Images from "Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients"

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    Journal: Viruses

    doi: 10.3390/v11010078

    Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Figure Legend Snippet: Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Techniques Used: Mutagenesis, Transfection, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).
    Figure Legend Snippet: Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Techniques Used: Mutagenesis, MANN-WHITNEY

    5) Product Images from "High rates of chronic HBV genotype E infection in a group of migrants in Italy from West Africa: Virological characteristics associated with poor immune clearance"

    Article Title: High rates of chronic HBV genotype E infection in a group of migrants in Italy from West Africa: Virological characteristics associated with poor immune clearance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0195045

    Graphs reporting the correlation between cccDNA and HBsAg titer (A) and serum HBV-DNA (B), and between intrahepatic total HBV-DNA and HBsAg titer (c) and serum HBV-DNA (D) in the 5 HBV genotype E infected patients with available liver biopsies.
    Figure Legend Snippet: Graphs reporting the correlation between cccDNA and HBsAg titer (A) and serum HBV-DNA (B), and between intrahepatic total HBV-DNA and HBsAg titer (c) and serum HBV-DNA (D) in the 5 HBV genotype E infected patients with available liver biopsies.

    Techniques Used: Infection

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: High rates of chronic HBV genotype E infection in a group of migrants in Italy from West Africa: Virological characteristics associated with poor immune clearance
    Article Snippet: .. Plasma HBV-DNA was quantified by real-time polymerase chain reaction (Roche Cobas® Ampliprep/Cobas® TaqMan). .. Most patients included in the study had a single point observation for HBV infection since they were in temporary migrant shelters and they moved to other locations after the first visit.

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients
    Article Snippet: .. The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL. .. HBV DNA was extracted from 200 μL serum samples using QIAamp DNA Blood Kit (Qiagen, Hilden, Germany).

    Article Title: HBcAb seropositivity is correlated with poor HIV viremia control in an Italian cohort of HIV/HBV-coinfected patients on first-line therapy
    Article Snippet: .. Plasma HBV-DNA was identified using real-time polymerase chain reaction (lower limit of quantification: 20 IU/ml) (Roche/Cobas Ampliprep/Cobas Taqman, Rotkreuz, Switzerland). ..

    Polymerase Chain Reaction:

    Article Title: Detection of Hepatitis B Virus (HBV) Genomes and HBV Drug Resistant Variants by Deep Sequencing Analysis of HBV Genomes in Immune Cell Subsets of HBV Mono-Infected and/or Human Immunodeficiency Virus Type-1 (HIV-1) and HBV Co-Infected Individuals
    Article Snippet: .. Follow-up blood samples were collected from 5/14 HBV mono-infected cases, of which 4/5 had started anti-HBV therapy (e.g. tenofovir or entecavir, median duration 22.6 months, range 16–32) with suppressed plasma HBV DNA as determined by a kinetic PCR assay (COBAS TaqMan HBV, Roche Molecular Systems). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Roche plasma hbv dna
    Comparison of <t>HBV</t> replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV <t>DNA</t> from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Plasma Hbv Dna, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma hbv dna/product/Roche
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    plasma hbv dna - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, Transfection, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, MANN-WHITNEY

    Graphs reporting the correlation between cccDNA and HBsAg titer (A) and serum HBV-DNA (B), and between intrahepatic total HBV-DNA and HBsAg titer (c) and serum HBV-DNA (D) in the 5 HBV genotype E infected patients with available liver biopsies.

    Journal: PLoS ONE

    Article Title: High rates of chronic HBV genotype E infection in a group of migrants in Italy from West Africa: Virological characteristics associated with poor immune clearance

    doi: 10.1371/journal.pone.0195045

    Figure Lengend Snippet: Graphs reporting the correlation between cccDNA and HBsAg titer (A) and serum HBV-DNA (B), and between intrahepatic total HBV-DNA and HBsAg titer (c) and serum HBV-DNA (D) in the 5 HBV genotype E infected patients with available liver biopsies.

    Article Snippet: Plasma HBV-DNA was quantified by real-time polymerase chain reaction (Roche Cobas® Ampliprep/Cobas® TaqMan).

    Techniques: Infection

    Alanine aminotransferase levels (ALT; left axis), log HBV DNA level (right axis), and log HIV RNA level (right axis) are presented over time. Treatment regimen and CD4 T-cell count at the time therapy was changed is also indicated.

    Journal: AIDS (London, England)

    Article Title: A CASE FOR TREATING HIGH HEPATITIS B DNA LEVELS PRIOR TO STARTING HIV THERAPY

    doi: 10.1097/QAD.0b013e3280110aef

    Figure Lengend Snippet: Alanine aminotransferase levels (ALT; left axis), log HBV DNA level (right axis), and log HIV RNA level (right axis) are presented over time. Treatment regimen and CD4 T-cell count at the time therapy was changed is also indicated.

    Article Snippet: Initial laboratory evaluation revealed: HBV surface antigen (HBsAg) positive, HBV core antibody (HBcAb) positive, HBV early antibody (HBeAb) positive, albumin 4.3 g/dL, bilirubin level 0.3 mg/dL, Aspartate aminotransferase (AST) 59 U/L, Alanine transaminase (ALT) 82 U/L, creatinine 0.95 mg/dL, and a plasma HBV DNA level of > 1,000,000,000 copies/mL (Roche COBAS TaqMan HBV Analyte Specific Reagent).

    Techniques: Cell Counting

    ccc and Total DNA Burden in Different Compartments A, intrahepatic cccDNA load in the liver tissue of treated and untreated patients; B, replicative index (RI) of HBV in the liver tissue of treated and untreated patients; C, HBV DNA content in peripheral blood leukocytes. Results are expressed as medians; boxes represent standard deviations. The 0 on the Y axis corresponds to “not detected”.

    Journal: Hepatitis Monthly

    Article Title: Simple and Reliable Method to Quantify the Hepatitis B Viral Load and Replicative Capacity in Liver Tissue and Blood Leukocytes

    doi: 10.5812/hepatmon.28751

    Figure Lengend Snippet: ccc and Total DNA Burden in Different Compartments A, intrahepatic cccDNA load in the liver tissue of treated and untreated patients; B, replicative index (RI) of HBV in the liver tissue of treated and untreated patients; C, HBV DNA content in peripheral blood leukocytes. Results are expressed as medians; boxes represent standard deviations. The 0 on the Y axis corresponds to “not detected”.

    Article Snippet: Plasma HBV DNA Quantitation The plasma total HBV DNA was measured using the COBAS AmpliPrep/COBAS TaqMan HBV test (Roche Molecular Diagnostics, Pleasanton, CA, USA).

    Techniques: Countercurrent Chromatography

    Distribution of Particle Associated HBV DNA in a Sucrose Density Gradient of HBV From a Plasma Sample A, tDNA; B, cccDNA; C, HBsAg; each fraction is indicated by a solid dot.

    Journal: Hepatitis Monthly

    Article Title: Simple and Reliable Method to Quantify the Hepatitis B Viral Load and Replicative Capacity in Liver Tissue and Blood Leukocytes

    doi: 10.5812/hepatmon.28751

    Figure Lengend Snippet: Distribution of Particle Associated HBV DNA in a Sucrose Density Gradient of HBV From a Plasma Sample A, tDNA; B, cccDNA; C, HBsAg; each fraction is indicated by a solid dot.

    Article Snippet: Plasma HBV DNA Quantitation The plasma total HBV DNA was measured using the COBAS AmpliPrep/COBAS TaqMan HBV test (Roche Molecular Diagnostics, Pleasanton, CA, USA).

    Techniques: