plasma corticosterone corticosterone levels  (Valiant)

Journal: bioRxiv

Article Title: Single exposure to social defeat or immobilization stress fails to induce lasting alterations in general anxiety and hippocampal neurogenesis in Wistar and wild-type Groningen rats

doi: 10.1101/2020.02.12.946186

Figure Lengend Snippet: Plasma corticosterone responses in the rats. A. illustrates responses in WTG rats and B. represents data from Wistar rats with different treatments (control, SSDS, AIS). The average corticosterone levels per treatment (ng/ml) were measured at 5 time points (−10, 15, 60, 120, 180 min). Duration of actual stress exposure is indicated on the X-axis (red: AIS and blue: SSDS).

Article Snippet: Analysis of plasma corticosterone Corticosterone levels were determined by radioimmunoassay (MP Biomedicals, Orangeburg, NY) with an intra-assay variation of 7.2% and an inter-assay variation of 6.9%.

Techniques:

Journal: Translational Psychiatry

Article Title: PhenoWorld: a new paradigm to screen rodent behavior

doi: 10.1038/tp.2014.40

Figure Lengend Snippet: Body weight ( a ) and body weight variation ( b ) of animals living in the PhenoWorld (PhW) compared to animals living in standard cages in groups of six or pair–housed (PhW, Std(6) or Std(2) respectively); dashed lines show that animals living in the same conditions lose weight when submitted to unpredictable chronic mild stress (uCMS) protocol (started at week 16-17 of age for PhW+CMS, Std(6)+CMS and Std(2)+CMS groups). The AnimalGates (AG) blue bar indicates the period when animals housed in the PhW adapted to use the AG; the sucrose preference test (SPT-b) gray bar and the uCMS black bar indicate, respectively, the periods when the baseline SPT and the uCMS protocol were performed. ( c ) Water and ( d ) food intake per day per animal. ( e , f ) Plasma corticosterone levels at baseline and six weeks after: CORT (8 h) indicates the time point when lights went on, and CORT (20 h) indicates the time point when lights went off; the red line on the graphs represents the detection limit for corticosterone measured by radioimmunoassay (25 ng ml –1 ). Error bars represent s.e.m.; •• P ⩽0.001 indicates the effect of time; * P ⩽0.05 and ** P ⩽0.001 denote the effect of housing; x P ⩽0.05 and xx P ⩽0.001 denote the effect of stress.

Article Snippet: Plasma corticosterone levels were determined using a commercial radioimmunoassay kit (ImmuChem, MP Biomedicals, LLC, Orangeburg, NY, USA), following the protocol of the supplier.

Techniques: Single-particle Tracking, RIA Assay

Journal: Science Advances

Article Title: Early-life sleep disruption increases parvalbumin in primary somatosensory cortex and impairs social bonding in prairie voles

doi: 10.1126/sciadv.aav5188

Figure Lengend Snippet: Experimental design. Summary of experiments conducted on ( A ) juvenile prairie voles only and ( B ) adult prairie voles that underwent ELSD or control conditions. RIA, radioimmunoassay; IHC, immunohistochemistry.

Article Snippet: Corticosterone radioimmunoassay Steroid levels in plasma were measured using a commercially available 125-I radioimmunoassay (RIA) kit (ImmuChem Double Antibody Corticosterone for rodents, MP Biomedicals, Solon, OH, USA) following routine procedures ( ).

Techniques: RIA Assay, Immunohistochemistry

Journal: PLoS ONE

Article Title: Transcriptional Effects of Glucocorticoid Receptors in the Dentate Gyrus Increase Anxiety-Related Behaviors

doi: 10.1371/journal.pone.0007704

Figure Lengend Snippet: Basal and stress-induced corticosterone secretion in Eno2 -ΔGR/EGFP bigenic mice. ( A ) Plasma corticosterone concentrations were determined in basal conditions from blood collected either one hour after light on (AM = 8 am) or one hour after light off (PM = 8 pm), these two time points correspond respectively to the lowest and highest levels of corticosterone during circadian secretion. Control (n = 15) and Eno2 -ΔGR/EGFP bigenic mice (n = 10). ( B ) Kinetics of glucocorticoid secretion in response to stress (30 minutes of forced exposure to an open field). Mice were bled several times related to each time point and blood samples were collected from control (n = 13) and Eno2 -ΔGR/EGFP bigenic mice (n = 10) 60 minutes before the beginning of the stress (t-60), at the end of the stress (t30), then 75, 120 and 180 minutes after the beginning of the stress. Insert represents the area under the curve; ** = P

Article Snippet: Corticosterone assay Plasma corticosterone levels were measured by radioimmunoassay (RIA) as described elsewhere using a highly specific corticosterone antiserum (MP Biomedicals, France).

Techniques: Mouse Assay