plant genomic dna extraction kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    TaKaRa plant genomic dna extraction kit
    Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pANDA35HK- dsHaAK expression cassettes used for <t>Arabidopsis</t> transformation. 35S pro, CaMV 35S promoter; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase II gene; HaAK , cDNA sequence of AK gene from H. armigera ; RB, right border; LB, left border; (B) Detection of HaAK in non-transformed control and transgenic plants by PCR. A 1,068 bp fragment of HaAK was amplified and 18s rDNA was served as a control. Lane M, <t>DNA</t> marker DL2,000; Lane 1, untransformed control; Lane 2-8, the transformants; (C) The wild and transgenic plants were grown on kanamycin-containing medium. The homozygous single-copy transgenic plants were selected through Mendelian segregation; (D) Northern blot detection of HaAK dsRNA in control and different transgenic lines. Lane 1, the non-transformed control; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11 and AtdsHaAK-12 ); Actin1 was used as a reference and the DIG labeled probe for Actin1 was obtained by PCR using primers as described for RT-PCR. (E) RT-PCR amplification of HaAK in control and transgenic plants. A 1,068 bp fragment of HaAK was amplified and Arabidopsis Actin1 gene was used as a control. Lane 1, untransformed plant; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11, AtdsHaAK-12 ); (F) Northern blot detection of small RNA fragments of HaAK in transgenic plants leaves . Lane 1, non-transformed plant; Lane 2-7, the transformants.
    Plant Genomic Dna Extraction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plant genomic dna extraction kit/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plant genomic dna extraction kit - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Silencing the HaAK Gene by Transgenic Plant-Mediated RNAi Impairs Larval Growth of Helicoverpa armigera"

    Article Title: Silencing the HaAK Gene by Transgenic Plant-Mediated RNAi Impairs Larval Growth of Helicoverpa armigera

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.10468

    Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pANDA35HK- dsHaAK expression cassettes used for Arabidopsis transformation. 35S pro, CaMV 35S promoter; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase II gene; HaAK , cDNA sequence of AK gene from H. armigera ; RB, right border; LB, left border; (B) Detection of HaAK in non-transformed control and transgenic plants by PCR. A 1,068 bp fragment of HaAK was amplified and 18s rDNA was served as a control. Lane M, DNA marker DL2,000; Lane 1, untransformed control; Lane 2-8, the transformants; (C) The wild and transgenic plants were grown on kanamycin-containing medium. The homozygous single-copy transgenic plants were selected through Mendelian segregation; (D) Northern blot detection of HaAK dsRNA in control and different transgenic lines. Lane 1, the non-transformed control; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11 and AtdsHaAK-12 ); Actin1 was used as a reference and the DIG labeled probe for Actin1 was obtained by PCR using primers as described for RT-PCR. (E) RT-PCR amplification of HaAK in control and transgenic plants. A 1,068 bp fragment of HaAK was amplified and Arabidopsis Actin1 gene was used as a control. Lane 1, untransformed plant; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11, AtdsHaAK-12 ); (F) Northern blot detection of small RNA fragments of HaAK in transgenic plants leaves . Lane 1, non-transformed plant; Lane 2-7, the transformants.
    Figure Legend Snippet: Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pANDA35HK- dsHaAK expression cassettes used for Arabidopsis transformation. 35S pro, CaMV 35S promoter; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase II gene; HaAK , cDNA sequence of AK gene from H. armigera ; RB, right border; LB, left border; (B) Detection of HaAK in non-transformed control and transgenic plants by PCR. A 1,068 bp fragment of HaAK was amplified and 18s rDNA was served as a control. Lane M, DNA marker DL2,000; Lane 1, untransformed control; Lane 2-8, the transformants; (C) The wild and transgenic plants were grown on kanamycin-containing medium. The homozygous single-copy transgenic plants were selected through Mendelian segregation; (D) Northern blot detection of HaAK dsRNA in control and different transgenic lines. Lane 1, the non-transformed control; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11 and AtdsHaAK-12 ); Actin1 was used as a reference and the DIG labeled probe for Actin1 was obtained by PCR using primers as described for RT-PCR. (E) RT-PCR amplification of HaAK in control and transgenic plants. A 1,068 bp fragment of HaAK was amplified and Arabidopsis Actin1 gene was used as a control. Lane 1, untransformed plant; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11, AtdsHaAK-12 ); (F) Northern blot detection of small RNA fragments of HaAK in transgenic plants leaves . Lane 1, non-transformed plant; Lane 2-7, the transformants.

    Techniques Used: Transgenic Assay, Expressing, Transformation Assay, Sequencing, Polymerase Chain Reaction, Amplification, Marker, Northern Blot, Labeling, Reverse Transcription Polymerase Chain Reaction

    2) Product Images from "Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers"

    Article Title: Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23051048

    Amplification profile of RAPD primer D15 in H. rhamnoides genotypes. The arrow indicates 885 bp, lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL2000 (Takara, Dalian, China).
    Figure Legend Snippet: Amplification profile of RAPD primer D15 in H. rhamnoides genotypes. The arrow indicates 885 bp, lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL2000 (Takara, Dalian, China).

    Techniques Used: Amplification, Marker

    Part of the RAPD screening products of bulk DNA from each of the 140 male and female H. rhamnoides samples via decamer primers (Lanes 1, 3, 5, 7, 9, 11, 13, and 15: female; Lanes 2, 4, 6, 8, 10, 12, 14, and 16: male). The female-specific 885 bp band is indicated with an arrow. M, DNA Marker DL2000 (Takara, Dalian, China).
    Figure Legend Snippet: Part of the RAPD screening products of bulk DNA from each of the 140 male and female H. rhamnoides samples via decamer primers (Lanes 1, 3, 5, 7, 9, 11, 13, and 15: female; Lanes 2, 4, 6, 8, 10, 12, 14, and 16: male). The female-specific 885 bp band is indicated with an arrow. M, DNA Marker DL2000 (Takara, Dalian, China).

    Techniques Used: Marker

    Amplification profile of the SCAR marker Hrcx-15 in H. rhamnoides , showing the 885 bp fragment, indicated with an arrow, in all female samples. Lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL500 (Takara, Dalian, China).
    Figure Legend Snippet: Amplification profile of the SCAR marker Hrcx-15 in H. rhamnoides , showing the 885 bp fragment, indicated with an arrow, in all female samples. Lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL500 (Takara, Dalian, China).

    Techniques Used: Amplification, Marker

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon
    Article Snippet: The leaves were then inoculated with cubic mycelial plugs (2–3 mm3 ) of R . solani and incubated at 23 °C under a photoperiod of 20 h light and 4 h dark for 3 d. Disease severity was evaluated from the amounts of fungal DNAs in the inoculated leaves, expressed as fungal biomass. .. Total DNAs were extracted from the inoculated leaves using a Nucleospin Plant II Kit (Takara Bio, Shiga, Japan), and qPCR for fungal DNAs (28 S rDNA) was performed using a SYBR Premix Ex Taq II (Takara Bio) employing the Applied Biosystems 7500 System (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Isolation:

    Article Title: The IE2 60-Kilodalton and 40-Kilodalton Proteins Are Dispensable for Human Cytomegalovirus Replication but Are Required for Efficient Delayed Early and Late Gene Expression and Production of Infectious Virus ▿
    Article Snippet: Real-time reverse transcription-PCR (RT-PCR) and data analysis were performed essentially as previously described using primers and probes directed against the HCMV IE1 72, IE2 86, UL83, and UL84 genes and the cellular housekeeping glucose-6-phosphate dehydrogenase (G6PD) gene ( ). .. RNA was isolated with a NucleoSpin II kit (Clontech, Mountain View, CA) and subsequently treated with DNase using a Turbo DNA-free kit (Ambion, Austin, TX) to remove any residual DNA contamination and to allow the analysis of unspliced viral transcripts. ..

    Article Title: Repression of GLUT4 expression by the endoplasmic reticulum stress response in 3T3-L1 adipocytes
    Article Snippet: Run-on expression levels were calculated after normalization with run-on 18S expression. .. Quantitative RT-PCRTotal RNA was isolated from cells using the Nucleospin II Kit (Clonetech, Mountain View, CA.). cDNA was synthesized and then amplified using the Brilliant SYBR Green QRT-PCR Master Mix Kit, 1-Step (Stratagene, La Jolla, CA) and template-specific primers. ..

    DNA Extraction:

    Article Title: Quality Protein Maize Based on Reducing Sulfur in Leaf Cells
    Article Snippet: Genomic DNA was isolated from maize leaves at the three- to four-leaf stage using a modified CTAB extraction method ( ). .. For extraction of genomic DNA from mature maize kernels, a portion of the kernel that is mostly endosperm with no embryo tissues were ground to a fine powder and subjected to DNA extraction with the Nucleospin Plant II kit (Takara Bio). .. Transgenic plants were screened for the presence of both RNAi and Ec PAPR transgenes using the primer pairs 5′-ACAACCACTACCTGAGCAC-3′/5′-ATTAAGCTTTGCAGGTCACTGGATTTTGG-3′ ( ) and 5′-CTCCCCATCCCTATTTGAACCC-3′/5′-GGTAGGTTTCCGGGAACAAGTA-3′, respectively.

    Article Title: Candelaria asiatica, an Ignored New Species from South Korea
    Article Snippet: .. DNA isolation, PCR, DNA sequencing, and sequence alignment Total genomic DNA was extracted from the newly collected specimens using the NucleoSpin Plant II Kit (Clontech Laboratories, Mountain View, CA) following the manufacturer’s instructions. .. The internal transcribed spacer (ITS) region and the large subunit of the ribosomal RNA (28S) were targeted via PCR using the primers pairs ITS4/ITS1F [ ] and LR0R/LR5 [ , ], respectively.

    Synthesized:

    Article Title: Repression of GLUT4 expression by the endoplasmic reticulum stress response in 3T3-L1 adipocytes
    Article Snippet: Run-on expression levels were calculated after normalization with run-on 18S expression. .. Quantitative RT-PCRTotal RNA was isolated from cells using the Nucleospin II Kit (Clonetech, Mountain View, CA.). cDNA was synthesized and then amplified using the Brilliant SYBR Green QRT-PCR Master Mix Kit, 1-Step (Stratagene, La Jolla, CA) and template-specific primers. ..

    Amplification:

    Article Title: Repression of GLUT4 expression by the endoplasmic reticulum stress response in 3T3-L1 adipocytes
    Article Snippet: Run-on expression levels were calculated after normalization with run-on 18S expression. .. Quantitative RT-PCRTotal RNA was isolated from cells using the Nucleospin II Kit (Clonetech, Mountain View, CA.). cDNA was synthesized and then amplified using the Brilliant SYBR Green QRT-PCR Master Mix Kit, 1-Step (Stratagene, La Jolla, CA) and template-specific primers. ..

    SYBR Green Assay:

    Article Title: Repression of GLUT4 expression by the endoplasmic reticulum stress response in 3T3-L1 adipocytes
    Article Snippet: Run-on expression levels were calculated after normalization with run-on 18S expression. .. Quantitative RT-PCRTotal RNA was isolated from cells using the Nucleospin II Kit (Clonetech, Mountain View, CA.). cDNA was synthesized and then amplified using the Brilliant SYBR Green QRT-PCR Master Mix Kit, 1-Step (Stratagene, La Jolla, CA) and template-specific primers. ..

    Quantitative RT-PCR:

    Article Title: Repression of GLUT4 expression by the endoplasmic reticulum stress response in 3T3-L1 adipocytes
    Article Snippet: Run-on expression levels were calculated after normalization with run-on 18S expression. .. Quantitative RT-PCRTotal RNA was isolated from cells using the Nucleospin II Kit (Clonetech, Mountain View, CA.). cDNA was synthesized and then amplified using the Brilliant SYBR Green QRT-PCR Master Mix Kit, 1-Step (Stratagene, La Jolla, CA) and template-specific primers. ..

    Polymerase Chain Reaction:

    Article Title: Candelaria asiatica, an Ignored New Species from South Korea
    Article Snippet: .. DNA isolation, PCR, DNA sequencing, and sequence alignment Total genomic DNA was extracted from the newly collected specimens using the NucleoSpin Plant II Kit (Clontech Laboratories, Mountain View, CA) following the manufacturer’s instructions. .. The internal transcribed spacer (ITS) region and the large subunit of the ribosomal RNA (28S) were targeted via PCR using the primers pairs ITS4/ITS1F [ ] and LR0R/LR5 [ , ], respectively.

    DNA Sequencing:

    Article Title: Candelaria asiatica, an Ignored New Species from South Korea
    Article Snippet: .. DNA isolation, PCR, DNA sequencing, and sequence alignment Total genomic DNA was extracted from the newly collected specimens using the NucleoSpin Plant II Kit (Clontech Laboratories, Mountain View, CA) following the manufacturer’s instructions. .. The internal transcribed spacer (ITS) region and the large subunit of the ribosomal RNA (28S) were targeted via PCR using the primers pairs ITS4/ITS1F [ ] and LR0R/LR5 [ , ], respectively.

    Sequencing:

    Article Title: Candelaria asiatica, an Ignored New Species from South Korea
    Article Snippet: .. DNA isolation, PCR, DNA sequencing, and sequence alignment Total genomic DNA was extracted from the newly collected specimens using the NucleoSpin Plant II Kit (Clontech Laboratories, Mountain View, CA) following the manufacturer’s instructions. .. The internal transcribed spacer (ITS) region and the large subunit of the ribosomal RNA (28S) were targeted via PCR using the primers pairs ITS4/ITS1F [ ] and LR0R/LR5 [ , ], respectively.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    TaKaRa plant genomic dna extraction kit
    Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pANDA35HK- dsHaAK expression cassettes used for <t>Arabidopsis</t> transformation. 35S pro, CaMV 35S promoter; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase II gene; HaAK , cDNA sequence of AK gene from H. armigera ; RB, right border; LB, left border; (B) Detection of HaAK in non-transformed control and transgenic plants by PCR. A 1,068 bp fragment of HaAK was amplified and 18s rDNA was served as a control. Lane M, <t>DNA</t> marker DL2,000; Lane 1, untransformed control; Lane 2-8, the transformants; (C) The wild and transgenic plants were grown on kanamycin-containing medium. The homozygous single-copy transgenic plants were selected through Mendelian segregation; (D) Northern blot detection of HaAK dsRNA in control and different transgenic lines. Lane 1, the non-transformed control; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11 and AtdsHaAK-12 ); Actin1 was used as a reference and the DIG labeled probe for Actin1 was obtained by PCR using primers as described for RT-PCR. (E) RT-PCR amplification of HaAK in control and transgenic plants. A 1,068 bp fragment of HaAK was amplified and Arabidopsis Actin1 gene was used as a control. Lane 1, untransformed plant; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11, AtdsHaAK-12 ); (F) Northern blot detection of small RNA fragments of HaAK in transgenic plants leaves . Lane 1, non-transformed plant; Lane 2-7, the transformants.
    Plant Genomic Dna Extraction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plant genomic dna extraction kit/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plant genomic dna extraction kit - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pANDA35HK- dsHaAK expression cassettes used for Arabidopsis transformation. 35S pro, CaMV 35S promoter; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase II gene; HaAK , cDNA sequence of AK gene from H. armigera ; RB, right border; LB, left border; (B) Detection of HaAK in non-transformed control and transgenic plants by PCR. A 1,068 bp fragment of HaAK was amplified and 18s rDNA was served as a control. Lane M, DNA marker DL2,000; Lane 1, untransformed control; Lane 2-8, the transformants; (C) The wild and transgenic plants were grown on kanamycin-containing medium. The homozygous single-copy transgenic plants were selected through Mendelian segregation; (D) Northern blot detection of HaAK dsRNA in control and different transgenic lines. Lane 1, the non-transformed control; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11 and AtdsHaAK-12 ); Actin1 was used as a reference and the DIG labeled probe for Actin1 was obtained by PCR using primers as described for RT-PCR. (E) RT-PCR amplification of HaAK in control and transgenic plants. A 1,068 bp fragment of HaAK was amplified and Arabidopsis Actin1 gene was used as a control. Lane 1, untransformed plant; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11, AtdsHaAK-12 ); (F) Northern blot detection of small RNA fragments of HaAK in transgenic plants leaves . Lane 1, non-transformed plant; Lane 2-7, the transformants.

    Journal: International Journal of Biological Sciences

    Article Title: Silencing the HaAK Gene by Transgenic Plant-Mediated RNAi Impairs Larval Growth of Helicoverpa armigera

    doi: 10.7150/ijbs.10468

    Figure Lengend Snippet: Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pANDA35HK- dsHaAK expression cassettes used for Arabidopsis transformation. 35S pro, CaMV 35S promoter; HPT, hygromycin phosphotralsferase gene; NPT II, neomycin phosphotransferase II gene; HaAK , cDNA sequence of AK gene from H. armigera ; RB, right border; LB, left border; (B) Detection of HaAK in non-transformed control and transgenic plants by PCR. A 1,068 bp fragment of HaAK was amplified and 18s rDNA was served as a control. Lane M, DNA marker DL2,000; Lane 1, untransformed control; Lane 2-8, the transformants; (C) The wild and transgenic plants were grown on kanamycin-containing medium. The homozygous single-copy transgenic plants were selected through Mendelian segregation; (D) Northern blot detection of HaAK dsRNA in control and different transgenic lines. Lane 1, the non-transformed control; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11 and AtdsHaAK-12 ); Actin1 was used as a reference and the DIG labeled probe for Actin1 was obtained by PCR using primers as described for RT-PCR. (E) RT-PCR amplification of HaAK in control and transgenic plants. A 1,068 bp fragment of HaAK was amplified and Arabidopsis Actin1 gene was used as a control. Lane 1, untransformed plant; Lane 2-9, the transformants (line AtdsHaAK-2 , AtdsHaAK-3, AtdsHaAK-6, AtdsHaAK-7, AtdsHaAK-8, AtdsHaAK-9, AtdsHaAK-11, AtdsHaAK-12 ); (F) Northern blot detection of small RNA fragments of HaAK in transgenic plants leaves . Lane 1, non-transformed plant; Lane 2-7, the transformants.

    Article Snippet: Molecular analysis of transgenic plants Total genomic DNA was isolated from the leaf tissues of Arabidopsis using a plant genomic DNA extraction kit (Takara, China).

    Techniques: Transgenic Assay, Expressing, Transformation Assay, Sequencing, Polymerase Chain Reaction, Amplification, Marker, Northern Blot, Labeling, Reverse Transcription Polymerase Chain Reaction

    Amplification profile of RAPD primer D15 in H. rhamnoides genotypes. The arrow indicates 885 bp, lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL2000 (Takara, Dalian, China).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

    doi: 10.3390/molecules23051048

    Figure Lengend Snippet: Amplification profile of RAPD primer D15 in H. rhamnoides genotypes. The arrow indicates 885 bp, lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL2000 (Takara, Dalian, China).

    Article Snippet: DNA Extraction The genomic DNA of the green leaves of all trees was isolated via the Takara Plant Genomic DNA Extraction Kit (Takara, Dalian, China), after grinding plant tissue in liquid nitrogen.

    Techniques: Amplification, Marker

    Part of the RAPD screening products of bulk DNA from each of the 140 male and female H. rhamnoides samples via decamer primers (Lanes 1, 3, 5, 7, 9, 11, 13, and 15: female; Lanes 2, 4, 6, 8, 10, 12, 14, and 16: male). The female-specific 885 bp band is indicated with an arrow. M, DNA Marker DL2000 (Takara, Dalian, China).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

    doi: 10.3390/molecules23051048

    Figure Lengend Snippet: Part of the RAPD screening products of bulk DNA from each of the 140 male and female H. rhamnoides samples via decamer primers (Lanes 1, 3, 5, 7, 9, 11, 13, and 15: female; Lanes 2, 4, 6, 8, 10, 12, 14, and 16: male). The female-specific 885 bp band is indicated with an arrow. M, DNA Marker DL2000 (Takara, Dalian, China).

    Article Snippet: DNA Extraction The genomic DNA of the green leaves of all trees was isolated via the Takara Plant Genomic DNA Extraction Kit (Takara, Dalian, China), after grinding plant tissue in liquid nitrogen.

    Techniques: Marker

    Amplification profile of the SCAR marker Hrcx-15 in H. rhamnoides , showing the 885 bp fragment, indicated with an arrow, in all female samples. Lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL500 (Takara, Dalian, China).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

    doi: 10.3390/molecules23051048

    Figure Lengend Snippet: Amplification profile of the SCAR marker Hrcx-15 in H. rhamnoides , showing the 885 bp fragment, indicated with an arrow, in all female samples. Lanes 1–12: male, lanes 13–24: female. M, DNA Marker DL500 (Takara, Dalian, China).

    Article Snippet: DNA Extraction The genomic DNA of the green leaves of all trees was isolated via the Takara Plant Genomic DNA Extraction Kit (Takara, Dalian, China), after grinding plant tissue in liquid nitrogen.

    Techniques: Amplification, Marker