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Promega placental rnasin
Placental Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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placental rnasin - by Bioz Stars, 2020-03
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Centrifugation:

Article Title: Intracellular Vesicle Acidification Promotes Maturation of Infectious Poliovirus Particles
Article Snippet: Cells were washed and harvested by scraping into 1 ml of a solution containing 10 mM Tris (pH 7.4), 10 mM NaCl, 1.5 mM MgCl2 , 1% Nonidet P-40, 1 uM phenylmethylsulfonyl fluoride (Sigma), and 40 U of placental RNasin (Promega) per ml. .. Nuclei were pelleted by centrifugation at 1,600×g for 10 min at 4°C, and 0.5 ml of the resulting cytoplasmic extract was loaded directly on 11-ml gradients containing 15 to 30% sucrose in 10 mM Tris (pH 7.4)-10 mM NaCl-1.5 mM MgCl2 .

Agarose Gel Electrophoresis:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate. .. The precipitated RNAs were then washed two times with 70% ethanol, resuspended in diethyl pyrocarbonate-treated water, and quantitated by agarose gel electrophoresis and ethidium bromide staining with known quantities of similar-sized RNAs.

In Vitro:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: The RibPVE2A(MluI) dicistronic replicon RNA used to program the in vitro translation-transcription reactions was generated by T7-based run-off transcription on a pT7RibPVE2A(MluI) template that was linearized with Mlu I. .. The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate.

Synthesized:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: .. The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate. .. The precipitated RNAs were then washed two times with 70% ethanol, resuspended in diethyl pyrocarbonate-treated water, and quantitated by agarose gel electrophoresis and ethidium bromide staining with known quantities of similar-sized RNAs.

Incubation:

Article Title: Intracellular Vesicle Acidification Promotes Maturation of Infectious Poliovirus Particles
Article Snippet: Analysis of assembly intermediates by sucrose gradients After 3 h of incubation, the cells were washed twice with DME lacking methionine (GIBCO), 3.0 ml of DME lacking methionine, containing 100 µCi of [35 S]methionine (ICN) per ml was added to each plate. .. Cells were washed and harvested by scraping into 1 ml of a solution containing 10 mM Tris (pH 7.4), 10 mM NaCl, 1.5 mM MgCl2 , 1% Nonidet P-40, 1 uM phenylmethylsulfonyl fluoride (Sigma), and 40 U of placental RNasin (Promega) per ml.

Electrophoretic Mobility Shift Assay:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: RNA probes for use in RNA electrophoretic mobility shift assays were generated by run-off transcription using bacteriophage T7 RNA polymerase as described previously ( ). .. The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate.

Purification:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: Paragraph title: Transcription and purification of RNA. ... The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate.

Generated:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: The RibPVE2A(MluI) dicistronic replicon RNA used to program the in vitro translation-transcription reactions was generated by T7-based run-off transcription on a pT7RibPVE2A(MluI) template that was linearized with Mlu I. .. The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate.

Activity Assay:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: Both probes were gel purified on an 8% polyacrylamide urea gel, and following overnight elution in elution buffer (0.5 M ammonium acetate, 1 mM EDTA, and 0.1% sodium dodecyl sulfate [SDS]) they were ethanol precipitated, resuspended in diethyl pyrocarbonate-treated water, and quantified based on specific activity. .. The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate.

Staining:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate. .. The precipitated RNAs were then washed two times with 70% ethanol, resuspended in diethyl pyrocarbonate-treated water, and quantitated by agarose gel electrophoresis and ethidium bromide staining with known quantities of similar-sized RNAs.

Plasmid Preparation:

Article Title: Distinct Poly(rC) Binding Protein KH Domain Determinants for Poliovirus Translation Initiation and Viral RNA Replication
Article Snippet: Briefly, pT7-5′NCR linearized with Dde I was used as template to generate the radiolabeled stem-loop I RNA probe (corresponding to the first 108 nt of PV). pT220-460 linearized with Hin dIII was used as template to generate the radiolabeled stem-loop IV RNA probe containing PV nt 220 to 460 preceded by 9 nt and followed by 6 nt of pGEM-1-derived vector sequences. .. The RNA was synthesized as described previously ( ) with the following exceptions: (i) the reaction volumes were 100 μl, (ii) 50 ng of Mlu I-linearized pT7RibPVE2A(MluI) transcription template was used per microliter of reaction mixture, (iii) 1.0 U of placental RNasin (Promega) was used per microliter of reaction mixture, (iv) 2.5 U of T7 RNA polymerase (New England Biolabs) was used per microliter of reaction mixture, and (v) the incubations were carried out at 37°C for 2.5 to 3 h. Following transcription, the RNAs were extracted with phenol-chloroform and ethanol precipitated in the presence of ammonium acetate.

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  • 79
    Promega human placenta rnasin hprnasin
    Inhibition of c- fos translation in the presence of anti-sense c- fos oligodeoxynudeotide. (A) A full length c- fos mRNA (0.6 μg) was translated using rabbit reticulocyte lysate and { 35 S}methionine with <t>RNasin</t> in the absence (lane 1 or in the presence of antisense oligodeoxynudeotide (10 pmol of anti-rncfosr 115 in lanes 2 and 3 or anti-rncfosr 143 in lanes 4 and 5). The peptide was immunoprecipitated by Ab-2 and protein A-agarose and then resolved in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (7.5%). Three molecular mass markers were depicted, and a single peptide of 62 kd was shown. The intensity of the 62-kd protein as measured using a laser densitometer was 100% (lane 1), 13% (lane 5), and at a level not distinguishable from the background (lanes 2–4). (B) A full-length c- fos mRNA (lanes 1–3) or a complementary c- fos mRNA (lane 4), each at 2.25 μg, was translated with the addition of <t>hpRNasin</t> and then the peptide was immunoprecipitated by Ab-2 and protein A-agarose as described in the text, except in the presence of 100 pmol unmodified sense-rncfosr 115 (lane 1), anti-rncfosr 115 (lane 2), or no oligodeoxynudeotide (lanes 3 and 4). Molecular mass markers were 80 and 49.5 kd.
    Human Placenta Rnasin Hprnasin, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human placenta rnasin hprnasin/product/Promega
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human placenta rnasin hprnasin - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    83
    Promega human placental rnase inhibitor
    Figure 4. <t>RNase</t> activity in the presence of human placental RNase inhibitor. RNase activity of ( A ) MN-IgG-RNase and ( B ) CTX-IgG-RNase was measured in the presence of RNase inhibitor. 10 −10 M RNase incubated with up to 50-fold molar excess of RI (RNasin). ( C ) Bovine RNase was tested as control.
    Human Placental Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human placental rnase inhibitor/product/Promega
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human placental rnase inhibitor - by Bioz Stars, 2020-03
    83/100 stars
      Buy from Supplier

    79
    Promega human placenta ribonuclease inhibitor gibcobrl
    Figure 4. <t>RNase</t> activity in the presence of human placental RNase inhibitor. RNase activity of ( A ) MN-IgG-RNase and ( B ) CTX-IgG-RNase was measured in the presence of RNase inhibitor. 10 −10 M RNase incubated with up to 50-fold molar excess of RI (RNasin). ( C ) Bovine RNase was tested as control.
    Human Placenta Ribonuclease Inhibitor Gibcobrl, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human placenta ribonuclease inhibitor gibcobrl/product/Promega
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human placenta ribonuclease inhibitor gibcobrl - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    98
    Promega human placenta ribonuclease inhibitor
    Figure 4. <t>RNase</t> activity in the presence of human placental RNase inhibitor. RNase activity of ( A ) MN-IgG-RNase and ( B ) CTX-IgG-RNase was measured in the presence of RNase inhibitor. 10 −10 M RNase incubated with up to 50-fold molar excess of RI (RNasin). ( C ) Bovine RNase was tested as control.
    Human Placenta Ribonuclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human placenta ribonuclease inhibitor/product/Promega
    Average 98 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    human placenta ribonuclease inhibitor - by Bioz Stars, 2020-03
    98/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of c- fos translation in the presence of anti-sense c- fos oligodeoxynudeotide. (A) A full length c- fos mRNA (0.6 μg) was translated using rabbit reticulocyte lysate and { 35 S}methionine with RNasin in the absence (lane 1 or in the presence of antisense oligodeoxynudeotide (10 pmol of anti-rncfosr 115 in lanes 2 and 3 or anti-rncfosr 143 in lanes 4 and 5). The peptide was immunoprecipitated by Ab-2 and protein A-agarose and then resolved in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (7.5%). Three molecular mass markers were depicted, and a single peptide of 62 kd was shown. The intensity of the 62-kd protein as measured using a laser densitometer was 100% (lane 1), 13% (lane 5), and at a level not distinguishable from the background (lanes 2–4). (B) A full-length c- fos mRNA (lanes 1–3) or a complementary c- fos mRNA (lane 4), each at 2.25 μg, was translated with the addition of hpRNasin and then the peptide was immunoprecipitated by Ab-2 and protein A-agarose as described in the text, except in the presence of 100 pmol unmodified sense-rncfosr 115 (lane 1), anti-rncfosr 115 (lane 2), or no oligodeoxynudeotide (lanes 3 and 4). Molecular mass markers were 80 and 49.5 kd.

    Journal: Annals of neurology

    Article Title: Suppression of Ischemia-induced Fos Expression and AP-1 Activity by an Antisense Oligodeoxynucleotide to c-fos mRNA

    doi: 10.1002/ana.410360405

    Figure Lengend Snippet: Inhibition of c- fos translation in the presence of anti-sense c- fos oligodeoxynudeotide. (A) A full length c- fos mRNA (0.6 μg) was translated using rabbit reticulocyte lysate and { 35 S}methionine with RNasin in the absence (lane 1 or in the presence of antisense oligodeoxynudeotide (10 pmol of anti-rncfosr 115 in lanes 2 and 3 or anti-rncfosr 143 in lanes 4 and 5). The peptide was immunoprecipitated by Ab-2 and protein A-agarose and then resolved in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (7.5%). Three molecular mass markers were depicted, and a single peptide of 62 kd was shown. The intensity of the 62-kd protein as measured using a laser densitometer was 100% (lane 1), 13% (lane 5), and at a level not distinguishable from the background (lanes 2–4). (B) A full-length c- fos mRNA (lanes 1–3) or a complementary c- fos mRNA (lane 4), each at 2.25 μg, was translated with the addition of hpRNasin and then the peptide was immunoprecipitated by Ab-2 and protein A-agarose as described in the text, except in the presence of 100 pmol unmodified sense-rncfosr 115 (lane 1), anti-rncfosr 115 (lane 2), or no oligodeoxynudeotide (lanes 3 and 4). Molecular mass markers were 80 and 49.5 kd.

    Article Snippet: Proteinase K, T4 polynucleotide kinase, RNase H, RNase A, double-stranded oligodeoxynucleotides with AP-1 (5′tccggc tgactca tcaagcg-3′), CREB (5′-ctagctctct gacgtca ggcaatctct-3′), SP-1 (5′-gctcgc cccgcc ccgatcgaat-3′) consensus sequences, in vitro translation kit with nuclease-treated rabbit reticulocyte lysate, recombinant RNasin (rRNasin), and human placenta RNasin (hpRNasin) were from Promega Biotech (Madison, WI).

    Techniques: Inhibition, Immunoprecipitation, Polyacrylamide Gel Electrophoresis

    Figure 4. RNase activity in the presence of human placental RNase inhibitor. RNase activity of ( A ) MN-IgG-RNase and ( B ) CTX-IgG-RNase was measured in the presence of RNase inhibitor. 10 −10 M RNase incubated with up to 50-fold molar excess of RI (RNasin). ( C ) Bovine RNase was tested as control.

    Journal: mAbs

    Article Title: Evaluation of human pancreatic RNase as effector molecule in a therapeutic antibody platform

    doi: 10.4161/mabs.27830

    Figure Lengend Snippet: Figure 4. RNase activity in the presence of human placental RNase inhibitor. RNase activity of ( A ) MN-IgG-RNase and ( B ) CTX-IgG-RNase was measured in the presence of RNase inhibitor. 10 −10 M RNase incubated with up to 50-fold molar excess of RI (RNasin). ( C ) Bovine RNase was tested as control.

    Article Snippet: In addition, RNase activity of MN-IgG-RNase was also tested in the presence of a concentration series of up to 50 fold molar excess of human placental RNase inhibitor (RNasin Plus, Promega).

    Techniques: Activity Assay, Incubation