3 kda molecular weight cutoff  (Millipore)


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    Structured Review

    Millipore 3 kda molecular weight cutoff
    3 Kda Molecular Weight Cutoff, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 kda molecular weight cutoff/product/Millipore
    Average 98 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    3 kda molecular weight cutoff - by Bioz Stars, 2020-01
    98/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: A double-gene construct was obtained by cloning the expression cassette of the heavy chain vector into pEE12.4. .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: Paragraph title: Cloning, overexpression and purification of METTL16_291 and METTL16 ... Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore).

    Centrifugation:

    Article Title: Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen
    Article Snippet: Primary antibodies in the culture supernatant of each clone were separated by centrifugation at 8,000 rpm for 15 min and eluted with AcroSep Hyper DF columns (Pall, New York, NY, USA). .. Samples were then further concentrated 10–20-fold using Amicon Ultra centrifugal filters (Millipore, Bedford, MA, USA).

    Article Title: SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum
    Article Snippet: .. Extracts were pooled and cleared one more time by centrifugation (5 min, 20,000 × g ), mixed with 3 mL of analytical grade water and filtered using Amicon Ultra centrifugal filters (3,000 Da MW cutoff regenerated cellulose membrane; EMD Millipore, Billerica, MA). .. An aliquot of the filtered extracts (1.5 mL) was dried under vacuum, re-suspended with 1 N HCl, and incubated at 95°C for 3 h. The mixture was subjected to three ethyl acetate partitioning steps.

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: Cell debris was cleared by centrifugation for 45 minutes at 10,000 g at 4°C. .. Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight.

    Filtration:

    Article Title: A Simple and Efficient Method for Generating High-quality Recombinant Mical Enzyme for In Vitro Assays
    Article Snippet: Conical centrifuge tubes were obtained from Corning Life Sciences (Corning, NY, USA), and Amicon Ultra centrifugal filters (Ultracel-50k) were obtained from Millipore, Billerica, MA, USA. .. HISTRAP FF, HIPREP 26/10 Desalting columns, Superdex™ 200, 10/300GL gel filtration column, and AKTA Purifier UPC 10 were from GE Healthcare Bio-Sciences Corporation (Piscataway, NJ, USA).

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Paragraph title: Antigen purification by gel filtration ... Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff).

    Mass Spectrometry:

    Article Title: SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum
    Article Snippet: Extracts were pooled and cleared one more time by centrifugation (5 min, 20,000 × g ), mixed with 3 mL of analytical grade water and filtered using Amicon Ultra centrifugal filters (3,000 Da MW cutoff regenerated cellulose membrane; EMD Millipore, Billerica, MA). .. Ethyl acetate fractions were pooled, dried in vacuo, and re-suspended in 50% (v/v) methanol-water (150 μL) prior to high-performance liquid chromatography (HPLC), electrospray ionization (ESI), and time-of-flight (TOF) mass spectrometry (MS) analysis.

    Diafiltration Assay:

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: .. Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein. .. Following a further diafiltration, PvTRAP-SATA was covalently linked to the derivatised CMVtt by reacting equimolar amounts of PvTRAP-SATA and CMVtt-SMPH for 4 h at room temperature (RT).

    Neutralization:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Cytometry:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Construct:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: A double-gene construct was obtained by cloning the expression cassette of the heavy chain vector into pEE12.4. .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: CMVtt-PvTRAP (Chemically Coupled) Vaccine Preparation The Phyto-VLP construct encoding the coat protein of cucumber mosaic virus fused to a universal T-cell epitope of the tetanus toxin (CMVtt) will be described in detail elsewhere (A. Zeltins, in preparation). .. Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein.

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa. .. Expression of the plasmid constructs involved induction of transformed Escherichia coli BL21 (DE3) cells with 1 m m isopropyl β- d -thiogalactopyranoside after growth on Luria-Bertani (LB) medium at 37 °C reached an A 600 of 0.6.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Antigen purification by gel filtration After an initial chromatography step, antigens to be used in ELISA and in the vaccination trials were further subjected to gel filtration to remove remaining impurities. .. Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff).

    Incubation:

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: .. Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein. .. Following a further diafiltration, PvTRAP-SATA was covalently linked to the derivatised CMVtt by reacting equimolar amounts of PvTRAP-SATA and CMVtt-SMPH for 4 h at room temperature (RT).

    Article Title: SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum
    Article Snippet: Extracts were pooled and cleared one more time by centrifugation (5 min, 20,000 × g ), mixed with 3 mL of analytical grade water and filtered using Amicon Ultra centrifugal filters (3,000 Da MW cutoff regenerated cellulose membrane; EMD Millipore, Billerica, MA). .. An aliquot of the filtered extracts (1.5 mL) was dried under vacuum, re-suspended with 1 N HCl, and incubated at 95°C for 3 h. The mixture was subjected to three ethyl acetate partitioning steps.

    Article Title: Bone Marrow-Derived Mesenchymal Stem Cells Drive Lymphangiogenesis
    Article Snippet: .. The supernatant of 24 h incubation was collected, centrifuged at 1,000 g for 10 min and concentred with Amicon Ultra Centrifugal Filters (Millipore, Carrigtwohill, Ireland) and aliquots of the conditioned medium were stored at −80°C until use. .. Tumor transplantation model Mice were anesthetized by intraperitoneal injection of ketamine hydrochloride (75 mg/kg body weight; CEVA, Bruxelles, Belgium) and xylazine (10 mg/kg body weight; VMD, Arendonk, Belgium) and LLC-Luc cells (5×104 cells) were inoculated alone (n = 30) or mixed with BM-MSC (2,5×105 cells) (n = 25).

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: The supernatant was transferred to a 50-ml column packed with 3 ml of pre-washed HisTrap HP resin (GE Healthcare) and incubated for 5 min at 4 °C. .. Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore).

    Diffusion-based Assay:

    Article Title: The Pseudomonas aeruginosa Isohexenyl Glutaconyl Coenzyme A Hydratase (AtuE) Is Upregulated in Citronellate-Grown Cells and Belongs to the Crotonase Family
    Article Snippet: For crystallization, AtuE was initially concentrated to ∼10 mg/ml with Amicon Ultra centrifugal filters (10,000-molecular-weight cutoff; Millipore) in 10 mM HEPES–NaOH, pH 7.0, buffer. .. Crystals were grown by the hanging-drop vapor diffusion method at 16°C using a well solution containing 0.2 M sodium iodide, 20% (vol/vol) polyethylene glycol 4000.

    Expressing:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: Paragraph title: DS7 Fab protein expression and characterization ... DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Paragraph title: Protein Expression and Purification and Peptide Synthesis ... Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa.

    Modification:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: The heavy chain vector was modified to contain an IgG1 constant chain with a stop codon immediately after the cysteine of the hinge disulfide. .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: The purification protocol was modified to include on-column thrombin digestion of the hexahistidine tag during the nickel-nitrilotriacetic acid purification step. .. Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa.

    Western Blot:

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein. .. The conjugated pVLP vaccine was analysed by SDS-PAGE and western blot to detect bands corresponding to the various components of the coupling reaction.

    Transformation Assay:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: The double-gene plasmid was transformed into DH5α E. coli cells (Invitrogen). .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa. .. Expression of the plasmid constructs involved induction of transformed Escherichia coli BL21 (DE3) cells with 1 m m isopropyl β- d -thiogalactopyranoside after growth on Luria-Bertani (LB) medium at 37 °C reached an A 600 of 0.6.

    Over Expression:

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: Paragraph title: Cloning, overexpression and purification of METTL16_291 and METTL16 ... Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore).

    Crystallization Assay:

    Article Title: The Pseudomonas aeruginosa Isohexenyl Glutaconyl Coenzyme A Hydratase (AtuE) Is Upregulated in Citronellate-Grown Cells and Belongs to the Crotonase Family
    Article Snippet: .. For crystallization, AtuE was initially concentrated to ∼10 mg/ml with Amicon Ultra centrifugal filters (10,000-molecular-weight cutoff; Millipore) in 10 mM HEPES–NaOH, pH 7.0, buffer. .. Crystals were grown by the hanging-drop vapor diffusion method at 16°C using a well solution containing 0.2 M sodium iodide, 20% (vol/vol) polyethylene glycol 4000.

    Derivative Assay:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. Antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. The antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    High Performance Liquid Chromatography:

    Article Title: SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum
    Article Snippet: Extracts were pooled and cleared one more time by centrifugation (5 min, 20,000 × g ), mixed with 3 mL of analytical grade water and filtered using Amicon Ultra centrifugal filters (3,000 Da MW cutoff regenerated cellulose membrane; EMD Millipore, Billerica, MA). .. Ethyl acetate fractions were pooled, dried in vacuo, and re-suspended in 50% (v/v) methanol-water (150 μL) prior to high-performance liquid chromatography (HPLC), electrospray ionization (ESI), and time-of-flight (TOF) mass spectrometry (MS) analysis.

    Article Title: A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid
    Article Snippet: Indole-3-acetamide, indole-3-acetonitrile, tryptamine, DL -indole-3-lactic acid, indole-3-ethanol and 3-kDa cut-off membrane centrifugal filters (Amicon Ultra 0.5 ml centrifugal filters Z677094) were obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA). .. HPLC-grade acetonitrile and methanol were purchased from POCH SA (Gliwice, Poland).

    Conjugation Assay:

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: .. Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein. .. Following a further diafiltration, PvTRAP-SATA was covalently linked to the derivatised CMVtt by reacting equimolar amounts of PvTRAP-SATA and CMVtt-SMPH for 4 h at room temperature (RT).

    Flow Cytometry:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Antigen purification by IMAC The GP4-Tm and GFP:GP4-Tm antigens were purified by IMAC on an Äkta Explorer® purification system fitted with a His Trap® Fast Flow column (1ml, GE healthcare). .. To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff).

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: The post-thrombin cleavage flow-through was subjected to size exclusion chromatography on a Hi-Load 16/60 Superdex 75 column (GE Healthcare) equilibrated with 10 m m HEPES, pH 7.4, 50 m m NaCl. .. Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa.

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: .. Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore). .. The protein was further purified by SEC (HiLoad 16/600 Superdex 200 pg, GE Healthcare) using size exclusion buffer (SEB) containing 25 mM HEPES pH 7.5 at RT, 100 mM KCl, 50 mM NaCl and 1 mM TCEP.

    Chromatography:

    Article Title: Expression pattern of arenicins—the antimicrobial peptides of polychaete Arenicola marina
    Article Snippet: Specific anti-arenicin antibody was purified from the serum by affine chromatography using CNBr-activated Sepharose (Cat. No. GE17-0430-01 Sigma-Aldrich, MO, USA) conjugated with recombinant arenicin-2. .. Anti-arenicin AB was eluted by NaCl gradient and desalted on Amicon Ultra centrifugal filters Ultracel 3K (Cat. No. Z677094-24EA, Millipore, Merck KGaA, Germany).

    Article Title: A Simple and Efficient Method for Generating High-quality Recombinant Mical Enzyme for In Vitro Assays
    Article Snippet: Paragraph title: Chemicals and Chromatography Resins ... Conical centrifuge tubes were obtained from Corning Life Sciences (Corning, NY, USA), and Amicon Ultra centrifugal filters (Ultracel-50k) were obtained from Millipore, Billerica, MA, USA.

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Antigen purification by gel filtration After an initial chromatography step, antigens to be used in ELISA and in the vaccination trials were further subjected to gel filtration to remove remaining impurities. .. Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff).

    Protease Inhibitor:

    Article Title: A Simple and Efficient Method for Generating High-quality Recombinant Mical Enzyme for In Vitro Assays
    Article Snippet: Basic chemicals were obtained from Sigma Chemical, Inc., St. Louis, MO, USA, while Complete® EDTA-free protease inhibitor cocktail tablets were obtained from Roche Applied Science, Indianapolis, IN, USA and Imidazole was obtained from EMD Biosciences, San Diego, CA, USA. .. Conical centrifuge tubes were obtained from Corning Life Sciences (Corning, NY, USA), and Amicon Ultra centrifugal filters (Ultracel-50k) were obtained from Millipore, Billerica, MA, USA.

    Cell Culture:

    Article Title: Bone Marrow-Derived Mesenchymal Stem Cells Drive Lymphangiogenesis
    Article Snippet: BM-MSC conditioned medium BM-MSC were seeded and cultured in mouse MesenCult medium (STEMCELL Technologies, Grenoble, France) until 90% confluence under mild hypoxic condition (5% O2 , 5% CO2 , 90% N2 ). .. The supernatant of 24 h incubation was collected, centrifuged at 1,000 g for 10 min and concentred with Amicon Ultra Centrifugal Filters (Millipore, Carrigtwohill, Ireland) and aliquots of the conditioned medium were stored at −80°C until use.

    Generated:

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa. .. A synthetic 24-residue MyoBIQ peptide comprising residues 693–716 of MyoB (Ac-KDFDCTAKIQKAFRNWKAKKHSLE-NH2 ) was generated for NMR mapping studies ( ).

    other:

    Article Title: Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile
    Article Snippet: The ligase was inactivated at 65°C for 10 min and the buffer was exchanged to H2 O using Amicon Ultra Centrifugal Filters (30K MWCO).

    Imaging:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. Antigen purity was determined by densitometric analysis of Coomassie-stained polyacrylamide gels using the aforementioned imaging system and the Image Lab 3.0 software (Biorad).

    Injection:

    Article Title: Expression pattern of arenicins—the antimicrobial peptides of polychaete Arenicola marina
    Article Snippet: The conjugate was compounded with complete Freund adjuvant (Cat. No. F5881, Sigma-Aldrich, MO, USA) and injected into rabbit males at a dose 1 μg per gram. .. Anti-arenicin AB was eluted by NaCl gradient and desalted on Amicon Ultra centrifugal filters Ultracel 3K (Cat. No. Z677094-24EA, Millipore, Merck KGaA, Germany).

    Binding Assay:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: After washing the column with binding buffer, antigens were eluted with 0.5 M imidazole (in binding buffer). .. To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff).

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: The column was connected to a Vac-Man Laboratory Vacuum Manifold (Promega) to wash the protein-bound resin six times with 50 ml binding buffer for each wash. .. Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore).

    Immunofluorescence:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Molecular Weight:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: .. To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. Antigen purity was determined by densitometric analysis of Coomassie-stained polyacrylamide gels using the aforementioned imaging system and the Image Lab 3.0 software (Biorad).

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: .. Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight. ..

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: .. Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. The antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    Transfection:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: Plasmid DNA was prepared using a Giga Kit (Qiagen) and transfected into 293F cells (Invitrogen) using PolyFect reagent (Qiagen) in a 10 liter WAVE bioreactor bag (GE). .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Purification:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Paragraph title: Antigen purification by IMAC ... To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff).

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: After one week, supernatant was purified over a gravity column with CaptureSelect λ resin (BAC B.V., The Netherlands) and eluted with citrate buffer. .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen
    Article Snippet: Paragraph title: Purification of mAbs ... Samples were then further concentrated 10–20-fold using Amicon Ultra centrifugal filters (Millipore, Bedford, MA, USA).

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: .. Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein. .. Following a further diafiltration, PvTRAP-SATA was covalently linked to the derivatised CMVtt by reacting equimolar amounts of PvTRAP-SATA and CMVtt-SMPH for 4 h at room temperature (RT).

    Article Title: Expression pattern of arenicins—the antimicrobial peptides of polychaete Arenicola marina
    Article Snippet: Specific anti-arenicin antibody was purified from the serum by affine chromatography using CNBr-activated Sepharose (Cat. No. GE17-0430-01 Sigma-Aldrich, MO, USA) conjugated with recombinant arenicin-2. .. Anti-arenicin AB was eluted by NaCl gradient and desalted on Amicon Ultra centrifugal filters Ultracel 3K (Cat. No. Z677094-24EA, Millipore, Merck KGaA, Germany).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Paragraph title: Protein Expression and Purification and Peptide Synthesis ... Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa.

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: .. Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight. ..

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: Paragraph title: Cloning, overexpression and purification of METTL16_291 and METTL16 ... Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore).

    Protein Purification:

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: Paragraph title: Protein purification ... Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight.

    Spectroscopy:

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight. .. Protein samples were evaluated for purity by SDS-PAGE ( ) and concentrations were determined using UV spectroscopy at 280 nm with calculated extinction coefficients : Sfp = 28880 M−1 cm−1 , hPPTase = 56950 M−1 cm−1 , VibB = 48930 M−1 cm−1 , hACP = 1490 M−1 cm−1 , ActACP = 2980 M−1 cm−1 .

    Lysis:

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: Cells thawed on ice were re-suspended in lysis buffer [50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, 5 μg/mL DNase I & 5 μg/mL RNAse A, 0.1 mg/mL lysozyme and lysed with either French pressure (Thermo Electron Corp/Thermo Scientific, Waltham, MA) or Model#110 F microfluidizer (Microfluidics Corporation, Newton, MA). .. Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight.

    SDS Page:

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein. .. The conjugated pVLP vaccine was analysed by SDS-PAGE and western blot to detect bands corresponding to the various components of the coupling reaction.

    Article Title: Fluorescent techniques for discovery and characterization of phosphopantetheinyl transferase inhibitors
    Article Snippet: Fractions containing the purified target proteins were dialyzed against 50 mM TrisCl pH 8.0, 500 mM NaCl, 1 mM DTT, the dialysate concentrated using Amicon-Ultra centrifugal filters (Millipore, Billerica, MA) appropriate for the target protein's molecular weight. .. Protein samples were evaluated for purity by SDS-PAGE ( ) and concentrations were determined using UV spectroscopy at 280 nm with calculated extinction coefficients : Sfp = 28880 M−1 cm−1 , hPPTase = 56950 M−1 cm−1 , VibB = 48930 M−1 cm−1 , hACP = 1490 M−1 cm−1 , ActACP = 2980 M−1 cm−1 .

    Plasmid Preparation:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: Plasmid DNA was prepared using a Giga Kit (Qiagen) and transfected into 293F cells (Invitrogen) using PolyFect reagent (Qiagen) in a 10 liter WAVE bioreactor bag (GE). .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa. .. Expression of the plasmid constructs involved induction of transformed Escherichia coli BL21 (DE3) cells with 1 m m isopropyl β- d -thiogalactopyranoside after growth on Luria-Bertani (LB) medium at 37 °C reached an A 600 of 0.6.

    Software:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. Antigen purity was determined by densitometric analysis of Coomassie-stained polyacrylamide gels using the aforementioned imaging system and the Image Lab 3.0 software (Biorad).

    Recombinant:

    Article Title: Expression pattern of arenicins—the antimicrobial peptides of polychaete Arenicola marina
    Article Snippet: Specific anti-arenicin antibody was purified from the serum by affine chromatography using CNBr-activated Sepharose (Cat. No. GE17-0430-01 Sigma-Aldrich, MO, USA) conjugated with recombinant arenicin-2. .. Anti-arenicin AB was eluted by NaCl gradient and desalted on Amicon Ultra centrifugal filters Ultracel 3K (Cat. No. Z677094-24EA, Millipore, Merck KGaA, Germany).

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Bacterial expression and purification of uniformly 13 C,15 N-labeled recombinant MlcB was performed as previously described by Crawley et al. ( ). .. Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa.

    In Vitro:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore). .. The mammalian cell-expressed DS7 Fab was tested for in vitro plaque reduction neutralization in comparison to the original bacterial-expressed DS7 IgG, and for binding to native HMPV F by immunofluorescence, immunoblot, and flow cytometry (not shown).

    Size-exclusion Chromatography:

    Article Title: Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodiumberghei/vivax
    Article Snippet: VLPs were expressed in Escherichia coli BL21 (DE3) Star (Thermo Fisher Scientific, Loughborough, UK) and purified from the soluble fraction of cell lysates by repeated precipitation with 40% (v/v) PEG8000 (Sigma-Aldrich, Gillingham, UK) and ammonium sulphate solution to yield soluble particles that were further purified by size exclusion chromatography (16/600 Sephacryl S-500HR, GE Healthcare, Life Sciences, Amersham, UK). .. Prior to the conjugation step, purified PvTRAP was incubated for 30 min at room temperature with a 6-fold molar excess of N-succinimidyl-S-acetylthioacetate (SATA) (Thermo Fisher Scientific); excess SATA cross-linker was removed by diafiltration as before using 3 kDa Amicon Ultra centrifugal filters (Millipore) and derivatised protein was then de-protected using hydroxylamine (3 h at room temperature) resulting in the addition of reactive sulphydryl residues to the protein.

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: The post-thrombin cleavage flow-through was subjected to size exclusion chromatography on a Hi-Load 16/60 Superdex 75 column (GE Healthcare) equilibrated with 10 m m HEPES, pH 7.4, 50 m m NaCl. .. Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa.

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore). .. The protein was further purified by SEC (HiLoad 16/600 Superdex 200 pg, GE Healthcare) using size exclusion buffer (SEB) containing 25 mM HEPES pH 7.5 at RT, 100 mM KCl, 50 mM NaCl and 1 mM TCEP.

    Homogenization:

    Article Title: A Simple and Efficient Method for Generating High-quality Recombinant Mical Enzyme for In Vitro Assays
    Article Snippet: Conical centrifuge tubes were obtained from Corning Life Sciences (Corning, NY, USA), and Amicon Ultra centrifugal filters (Ultracel-50k) were obtained from Millipore, Billerica, MA, USA. .. Homogenization was performed with a high-pressure homogenizer (EmulsiFlex-C5, Avestin, Inc., Ottawa, Ontario, Canada).

    Nuclear Magnetic Resonance:

    Article Title: Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ Motif Recognition *
    Article Snippet: Fractions eluting at 60–80 ml were pooled and concentrated at 4 °C using Amicon Ultra centrifugal filters (Millipore Corp.) with a molecular mass cut off of 3 kDa. .. A synthetic 24-residue MyoBIQ peptide comprising residues 693–716 of MyoB (Ac-KDFDCTAKIQKAFRNWKAKKHSLE-NH2 ) was generated for NMR mapping studies ( ).

    Spectrophotometry:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. Antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. The antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    Concentration Assay:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: To remove the imidazole, the antigens were washed with PBS on Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. Antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    Article Title: Structural insights into the RNA methyltransferase domain of METTL16
    Article Snippet: The imidazole concentration was lowered to 20 mM and the His6 -tag was cleaved with TEV protease (final concentration 0.1 mg/mL) during dialysis for 18 h at 4 °C. .. Flow-through was collected and concentrated to 2.0 ml using Amicon ultra centrifugal filters (30-kDa cutoff, Millipore).

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. The antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

    BAC Assay:

    Article Title: Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site
    Article Snippet: After one week, supernatant was purified over a gravity column with CaptureSelect λ resin (BAC B.V., The Netherlands) and eluted with citrate buffer. .. DS7 Fab was concentrated with Amicon Ultra centrifugal filters with a 30 kD molecular weight cut-off (Millipore).

    Staining:

    Article Title: Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity
    Article Snippet: .. Removal of impurities and/or degradation products was assayed by Coomassie staining of polyacrylamide gels and whenever necessary, pure, gel-filtrated antigen was concentrated using Amicon® Ultra centrifugal filters (Millipore, 3 kDa molecular weight cutoff). .. The antigen concentration was derived from the sample optical density at 280 nm measured by a NanoDrop® ND-1000 spectrophotometer.

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