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Becton Dickinson pkl lux interaction
<t>LUX</t> and <t>PKL</t> Regulate H3K27me3 Levels at the DOG1 Locus. (A) ChIP assay. PKL antibody was used to pull down different fragments of DOG1 (shown in Figure 2 C) and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. (B) ChIP assay. H3K27me3 antibody was used to pull down different fragments of DOG1 and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. Seedlings were grown under LD conditions for 5 d and samples were harvested at ZT4. Relative enrichment using the H3K27me3 antibody was normalized to that using the H3 antibody. In all experiments, values denote average ± SD of three biological replicates.
Pkl Lux Interaction, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis"

Article Title: The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis

Journal: Plant Communications

doi: 10.1016/j.xplc.2019.100011

LUX and PKL Regulate H3K27me3 Levels at the DOG1 Locus. (A) ChIP assay. PKL antibody was used to pull down different fragments of DOG1 (shown in Figure 2 C) and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. (B) ChIP assay. H3K27me3 antibody was used to pull down different fragments of DOG1 and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. Seedlings were grown under LD conditions for 5 d and samples were harvested at ZT4. Relative enrichment using the H3K27me3 antibody was normalized to that using the H3 antibody. In all experiments, values denote average ± SD of three biological replicates.
Figure Legend Snippet: LUX and PKL Regulate H3K27me3 Levels at the DOG1 Locus. (A) ChIP assay. PKL antibody was used to pull down different fragments of DOG1 (shown in Figure 2 C) and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. (B) ChIP assay. H3K27me3 antibody was used to pull down different fragments of DOG1 and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. Seedlings were grown under LD conditions for 5 d and samples were harvested at ZT4. Relative enrichment using the H3K27me3 antibody was normalized to that using the H3 antibody. In all experiments, values denote average ± SD of three biological replicates.

Techniques Used: Chromatin Immunoprecipitation

PKL Interacts with LUX. (A) Diagram of the PKL domains and various deletions. Numbers indicate amino acid positions. (B) Yeast two-hybrid assay. Full-length PKL and its deletion variants were fused with the LexA DNA-binding domain (BD-fusion), and LUX, ELF3, and ELF4 were tagged with the B42 activation domain (AD-fusion). Blue colonies denote protein–protein interactions. (C and D) Pull-down assay. D6-His recombinant protein was incubated with GST-LUX (C) or MBP-LUX (D) and immunoprecipitated by anti-GST or anti-MBP antibodies, respectively. (E) LCI assay. Full-length PKL was fused in-frame with the N terminus of LUC and LUX, ELF3, and ELF4 were fused in-frame the C terminus of LUC. Different plasmid compositions were cotransformed into N. benthamiana leaves.
Figure Legend Snippet: PKL Interacts with LUX. (A) Diagram of the PKL domains and various deletions. Numbers indicate amino acid positions. (B) Yeast two-hybrid assay. Full-length PKL and its deletion variants were fused with the LexA DNA-binding domain (BD-fusion), and LUX, ELF3, and ELF4 were tagged with the B42 activation domain (AD-fusion). Blue colonies denote protein–protein interactions. (C and D) Pull-down assay. D6-His recombinant protein was incubated with GST-LUX (C) or MBP-LUX (D) and immunoprecipitated by anti-GST or anti-MBP antibodies, respectively. (E) LCI assay. Full-length PKL was fused in-frame with the N terminus of LUC and LUX, ELF3, and ELF4 were fused in-frame the C terminus of LUC. Different plasmid compositions were cotransformed into N. benthamiana leaves.

Techniques Used: Y2H Assay, Binding Assay, Activation Assay, Pull Down Assay, Recombinant, Incubation, Immunoprecipitation, Plasmid Preparation

LUX and ELF3 Affect Circadian Output to Seeds. (A) Relative DOG1 expression in seedlings under free-running conditions. Seedlings were grown under 12 h light/12 h dark for 6 d followed by CL illumination for 24 h. Samples were harvested every 4 h from ZT24. (B) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (6 d after pollination) were harvested every 4 h started from ZT0. (C) Seed germination rate. Col-0, lux , and elf3 plants were grown under LD conditions for 3 weeks and transferred to CL or kept at LD until seed maturation. Germination of freshly harvested seeds in the light was analyzed. (D) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (8 d after pollination) were harvested at ZT8. For (A) , (B) , and (D) , data are the average ± SD of three biological replicates. (E) A working model illustrating the roles of PKL and EC in controlling seed dormancy. LUX binds directly to a specific DNA sequence of DOG1 and recruits PKL to the DOG1 locus through their physical interaction. This interaction increases H3K27me3 levels on DOG1 chromatin, thereby repressing its transcription and leading to reduced seed dormancy. Arrow indicates positive regulation and bar denotes negative regulation.
Figure Legend Snippet: LUX and ELF3 Affect Circadian Output to Seeds. (A) Relative DOG1 expression in seedlings under free-running conditions. Seedlings were grown under 12 h light/12 h dark for 6 d followed by CL illumination for 24 h. Samples were harvested every 4 h from ZT24. (B) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (6 d after pollination) were harvested every 4 h started from ZT0. (C) Seed germination rate. Col-0, lux , and elf3 plants were grown under LD conditions for 3 weeks and transferred to CL or kept at LD until seed maturation. Germination of freshly harvested seeds in the light was analyzed. (D) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (8 d after pollination) were harvested at ZT8. For (A) , (B) , and (D) , data are the average ± SD of three biological replicates. (E) A working model illustrating the roles of PKL and EC in controlling seed dormancy. LUX binds directly to a specific DNA sequence of DOG1 and recruits PKL to the DOG1 locus through their physical interaction. This interaction increases H3K27me3 levels on DOG1 chromatin, thereby repressing its transcription and leading to reduced seed dormancy. Arrow indicates positive regulation and bar denotes negative regulation.

Techniques Used: Expressing, Sequencing

Related Articles

Activation Assay:

Article Title: The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis
Article Snippet: .. To confirm the PKL-LUX interaction, we fused LUX with the B42 activation domain (AD) and full-length PKL or various PKL fragments with the LexA DNA-binding domain (BD) ( A). ..

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    Becton Dickinson pkl lux interaction
    <t>LUX</t> and <t>PKL</t> Regulate H3K27me3 Levels at the DOG1 Locus. (A) ChIP assay. PKL antibody was used to pull down different fragments of DOG1 (shown in Figure 2 C) and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. (B) ChIP assay. H3K27me3 antibody was used to pull down different fragments of DOG1 and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. Seedlings were grown under LD conditions for 5 d and samples were harvested at ZT4. Relative enrichment using the H3K27me3 antibody was normalized to that using the H3 antibody. In all experiments, values denote average ± SD of three biological replicates.
    Pkl Lux Interaction, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pkl lux interaction/product/Becton Dickinson
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pkl lux interaction - by Bioz Stars, 2021-02
    90/100 stars
      Buy from Supplier

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    LUX and PKL Regulate H3K27me3 Levels at the DOG1 Locus. (A) ChIP assay. PKL antibody was used to pull down different fragments of DOG1 (shown in Figure 2 C) and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. (B) ChIP assay. H3K27me3 antibody was used to pull down different fragments of DOG1 and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. Seedlings were grown under LD conditions for 5 d and samples were harvested at ZT4. Relative enrichment using the H3K27me3 antibody was normalized to that using the H3 antibody. In all experiments, values denote average ± SD of three biological replicates.

    Journal: Plant Communications

    Article Title: The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis

    doi: 10.1016/j.xplc.2019.100011

    Figure Lengend Snippet: LUX and PKL Regulate H3K27me3 Levels at the DOG1 Locus. (A) ChIP assay. PKL antibody was used to pull down different fragments of DOG1 (shown in Figure 2 C) and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. (B) ChIP assay. H3K27me3 antibody was used to pull down different fragments of DOG1 and the ACT2 control from Col-0, lux-6 , and pkl-1 plants. Seedlings were grown under LD conditions for 5 d and samples were harvested at ZT4. Relative enrichment using the H3K27me3 antibody was normalized to that using the H3 antibody. In all experiments, values denote average ± SD of three biological replicates.

    Article Snippet: To confirm the PKL-LUX interaction, we fused LUX with the B42 activation domain (AD) and full-length PKL or various PKL fragments with the LexA DNA-binding domain (BD) ( A).

    Techniques: Chromatin Immunoprecipitation

    PKL Interacts with LUX. (A) Diagram of the PKL domains and various deletions. Numbers indicate amino acid positions. (B) Yeast two-hybrid assay. Full-length PKL and its deletion variants were fused with the LexA DNA-binding domain (BD-fusion), and LUX, ELF3, and ELF4 were tagged with the B42 activation domain (AD-fusion). Blue colonies denote protein–protein interactions. (C and D) Pull-down assay. D6-His recombinant protein was incubated with GST-LUX (C) or MBP-LUX (D) and immunoprecipitated by anti-GST or anti-MBP antibodies, respectively. (E) LCI assay. Full-length PKL was fused in-frame with the N terminus of LUC and LUX, ELF3, and ELF4 were fused in-frame the C terminus of LUC. Different plasmid compositions were cotransformed into N. benthamiana leaves.

    Journal: Plant Communications

    Article Title: The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis

    doi: 10.1016/j.xplc.2019.100011

    Figure Lengend Snippet: PKL Interacts with LUX. (A) Diagram of the PKL domains and various deletions. Numbers indicate amino acid positions. (B) Yeast two-hybrid assay. Full-length PKL and its deletion variants were fused with the LexA DNA-binding domain (BD-fusion), and LUX, ELF3, and ELF4 were tagged with the B42 activation domain (AD-fusion). Blue colonies denote protein–protein interactions. (C and D) Pull-down assay. D6-His recombinant protein was incubated with GST-LUX (C) or MBP-LUX (D) and immunoprecipitated by anti-GST or anti-MBP antibodies, respectively. (E) LCI assay. Full-length PKL was fused in-frame with the N terminus of LUC and LUX, ELF3, and ELF4 were fused in-frame the C terminus of LUC. Different plasmid compositions were cotransformed into N. benthamiana leaves.

    Article Snippet: To confirm the PKL-LUX interaction, we fused LUX with the B42 activation domain (AD) and full-length PKL or various PKL fragments with the LexA DNA-binding domain (BD) ( A).

    Techniques: Y2H Assay, Binding Assay, Activation Assay, Pull Down Assay, Recombinant, Incubation, Immunoprecipitation, Plasmid Preparation

    LUX and ELF3 Affect Circadian Output to Seeds. (A) Relative DOG1 expression in seedlings under free-running conditions. Seedlings were grown under 12 h light/12 h dark for 6 d followed by CL illumination for 24 h. Samples were harvested every 4 h from ZT24. (B) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (6 d after pollination) were harvested every 4 h started from ZT0. (C) Seed germination rate. Col-0, lux , and elf3 plants were grown under LD conditions for 3 weeks and transferred to CL or kept at LD until seed maturation. Germination of freshly harvested seeds in the light was analyzed. (D) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (8 d after pollination) were harvested at ZT8. For (A) , (B) , and (D) , data are the average ± SD of three biological replicates. (E) A working model illustrating the roles of PKL and EC in controlling seed dormancy. LUX binds directly to a specific DNA sequence of DOG1 and recruits PKL to the DOG1 locus through their physical interaction. This interaction increases H3K27me3 levels on DOG1 chromatin, thereby repressing its transcription and leading to reduced seed dormancy. Arrow indicates positive regulation and bar denotes negative regulation.

    Journal: Plant Communications

    Article Title: The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis

    doi: 10.1016/j.xplc.2019.100011

    Figure Lengend Snippet: LUX and ELF3 Affect Circadian Output to Seeds. (A) Relative DOG1 expression in seedlings under free-running conditions. Seedlings were grown under 12 h light/12 h dark for 6 d followed by CL illumination for 24 h. Samples were harvested every 4 h from ZT24. (B) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (6 d after pollination) were harvested every 4 h started from ZT0. (C) Seed germination rate. Col-0, lux , and elf3 plants were grown under LD conditions for 3 weeks and transferred to CL or kept at LD until seed maturation. Germination of freshly harvested seeds in the light was analyzed. (D) Relative DOG1 expression in developing siliques. Plants were grown under LD conditions, and siliques (8 d after pollination) were harvested at ZT8. For (A) , (B) , and (D) , data are the average ± SD of three biological replicates. (E) A working model illustrating the roles of PKL and EC in controlling seed dormancy. LUX binds directly to a specific DNA sequence of DOG1 and recruits PKL to the DOG1 locus through their physical interaction. This interaction increases H3K27me3 levels on DOG1 chromatin, thereby repressing its transcription and leading to reduced seed dormancy. Arrow indicates positive regulation and bar denotes negative regulation.

    Article Snippet: To confirm the PKL-LUX interaction, we fused LUX with the B42 activation domain (AD) and full-length PKL or various PKL fragments with the LexA DNA-binding domain (BD) ( A).

    Techniques: Expressing, Sequencing