vectastain elite abc  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    VECTASTAIN Elite ABC Universal PLUS Kit Peroxidase Horse Anti Mouse Rabbit IgG
    Description:
    VECTASTAIN Elite ABC Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes
    Catalog Number:
    pk-8200
    Price:
    None
    Size:
    1 Kit
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    Universal Mouse Rabbit
    Buy from Supplier


    Structured Review

    Vector Laboratories vectastain elite abc
    VECTASTAIN Elite ABC Universal PLUS Kit Peroxidase Horse Anti Mouse Rabbit IgG
    VECTASTAIN Elite ABC Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes
    https://www.bioz.com/result/vectastain elite abc/product/Vector Laboratories
    Average 96 stars, based on 5011 article reviews
    Price from $9.99 to $1999.99
    vectastain elite abc - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation"

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21093179

    Histopathological changes in the lung tissues of the wild type mice intratracheally instilled with pSUD-FL, pSUD-NM, and pSUD-MC. The sections of the lung tissues from the instilled mice were performed using immunohistochemistry staining with anti-SARS-CoV SUD (( A ), first and second rows), or anti-mouse CD11b (( A ), third and fourth rows), and biotinylated universal antibodies from a VECTASTAIN ® Elite ® ABC Universal Kit ( A ). The sections were also assessed with H E staining, which the wide range of lung tissues was observed by a light microscope at 40× and 200× magnification (( B ), first and second rows); inflammatory cells infiltrated around the peribranchial and perivascular, and cell accumulation in alveolar space were examined using a light microscope at 400× magnification (( B ), third and fourth rows). The indices of histopathological changes in aggregation ( C ), perivascular cuffing ( D ), inflammation ( E ), and pathological index ( F ) were scored based on the degree of lesions ranged from one to five depending on severity: 1 = minimal (
    Figure Legend Snippet: Histopathological changes in the lung tissues of the wild type mice intratracheally instilled with pSUD-FL, pSUD-NM, and pSUD-MC. The sections of the lung tissues from the instilled mice were performed using immunohistochemistry staining with anti-SARS-CoV SUD (( A ), first and second rows), or anti-mouse CD11b (( A ), third and fourth rows), and biotinylated universal antibodies from a VECTASTAIN ® Elite ® ABC Universal Kit ( A ). The sections were also assessed with H E staining, which the wide range of lung tissues was observed by a light microscope at 40× and 200× magnification (( B ), first and second rows); inflammatory cells infiltrated around the peribranchial and perivascular, and cell accumulation in alveolar space were examined using a light microscope at 400× magnification (( B ), third and fourth rows). The indices of histopathological changes in aggregation ( C ), perivascular cuffing ( D ), inflammation ( E ), and pathological index ( F ) were scored based on the degree of lesions ranged from one to five depending on severity: 1 = minimal (

    Techniques Used: Mouse Assay, Immunohistochemistry, Staining, Light Microscopy

    Related Articles

    Avidin-Biotin Assay:

    Article Title: Kainate receptors are primarily postsynaptic to SP-containing axon terminals in the trigeminal dorsal horn
    Article Snippet: .. Bound primary antibody was visualized by incubating tissue sections in biotinylated donkey α rat IgG (1:400; Vector Laboratories, Burlingame, CA) or biotinylated donkey α mouse IgM (1:400; Jackson ImmunoResearch, West Grove, PA) secondary antibody followed by incubation in Avidin-Biotin (Elite Vectastain ABC kit; Vector Laboratories) and diaminobenzidine-hydrogen peroxidase (DAB-H2 O2 ) solutions. ..

    Article Title: Use of c-fos to identify activity-dependent spinal neurons after stepping in intact adult rats
    Article Snippet: .. The sections were incubated at RT for 1 h in 1:200 Vectastain Elite Avidin-Biotin Peroxidase Complex (ABC) solution (Vector Laboratories, Burlingame, CA, USA), then rinsed again in PBS and placed in diaminobenzidine (DAB) solution (Sigma-Aldrich Corp., St Louis, MO, USA) for 15 min for the chromogen reaction to occur. ..

    Article Title: Functional recovery of stepping in rats after a complete neonatal spinal cord transection is not due to regrowth across the lesion site
    Article Snippet: .. To detect BDA-labeling, free-floating spinal cord sections were first incubated in 0.3% H2 O2 in phosphate buffered saline solution (PBS) (15 min), rinsed in PBS, and incubated in Vectastain Elite avidin-biotin-peroxidase (ABC) complex (1:200 in PBS for 1 h; Vector Labs, Burlingame, CA). .. Following ABC incubation, the sections were rinsed in PBS, incubated in diaminobenzidine (DAB) (SigmaFast DAB kit for 20 min; Sigma-Aldrich, St. Louis, MO), and stored in PBS.

    Article Title: Anatomy and Physiology of Neurons in Layer 9 of the Chicken Optic Tectum
    Article Snippet: .. The tissue was then incubated in avidin-biotin peroxidase complex (ABC; Vectastain Elite ABC Kit, Vector Laboratories Inc., Burlingame, CA, USA; 3.2 ml/ml) in phosphate buffered saline (PBS)-Tx100 (0.5%/4% NaCl) for 1 h, washed three times in PB, moved to a solution of 0.0001% biotin-tyramide (IRIS Biotech GmbH, Marktredwitz, Germany) and 0.003% H2 O2 in 0.05 M borate buffer (pH 8.5) for 1 h, washed again three times in PB and incubated in the same ABC-solution as before for 1 h. After washing in PB and acetate imidazole buffer (AIP, 0.175 M acetate, 0.069% imidazole, pH 7.4/6.5), tissue was pre-incubated in a 0.025% diaminobenzidine solution (DAB-buffer tablets for microscopy, Merck KGaA, Darmstadt, Germany) with 1% NiSO4 in AIP (pH 6.5) for 5 min and the chromogenic reaction was induced by adding H2 O2 (end concentration 0.0025%) for 3 min. After washing the tissue, the sections were mounted on gelatin-coated slides, counterstained using neutral red, and coverslipped using DPX (DPX Mountant for Histology, Sigma-Aldrich GmbH, Steinheim, Germany). .. The brains from in vivo tracing experiments were treated in the same way but were sliced in 50 μm sections and the TSA steps were omitted.

    Article Title: TDP-43 pathology disrupts nuclear pore complexes and nucleocytoplasmic transport in ALS/FTD
    Article Snippet: .. The following day sections were incubated in biotinylated secondary antibody at 5 μL/mL (Elite IgG Vectastain ABC kit) for 30 mins at 37°C and then incubated with the avidin-biotin enzyme complex (Vector Laboratories) for 30 mins. .. Stains were visualized by incubation of DAB Chromogen (Sigma-Aldrich) for 5 mins at RT.

    Microscopy:

    Article Title: Anatomy and Physiology of Neurons in Layer 9 of the Chicken Optic Tectum
    Article Snippet: .. The tissue was then incubated in avidin-biotin peroxidase complex (ABC; Vectastain Elite ABC Kit, Vector Laboratories Inc., Burlingame, CA, USA; 3.2 ml/ml) in phosphate buffered saline (PBS)-Tx100 (0.5%/4% NaCl) for 1 h, washed three times in PB, moved to a solution of 0.0001% biotin-tyramide (IRIS Biotech GmbH, Marktredwitz, Germany) and 0.003% H2 O2 in 0.05 M borate buffer (pH 8.5) for 1 h, washed again three times in PB and incubated in the same ABC-solution as before for 1 h. After washing in PB and acetate imidazole buffer (AIP, 0.175 M acetate, 0.069% imidazole, pH 7.4/6.5), tissue was pre-incubated in a 0.025% diaminobenzidine solution (DAB-buffer tablets for microscopy, Merck KGaA, Darmstadt, Germany) with 1% NiSO4 in AIP (pH 6.5) for 5 min and the chromogenic reaction was induced by adding H2 O2 (end concentration 0.0025%) for 3 min. After washing the tissue, the sections were mounted on gelatin-coated slides, counterstained using neutral red, and coverslipped using DPX (DPX Mountant for Histology, Sigma-Aldrich GmbH, Steinheim, Germany). .. The brains from in vivo tracing experiments were treated in the same way but were sliced in 50 μm sections and the TSA steps were omitted.

    Concentration Assay:

    Article Title: Anatomy and Physiology of Neurons in Layer 9 of the Chicken Optic Tectum
    Article Snippet: .. The tissue was then incubated in avidin-biotin peroxidase complex (ABC; Vectastain Elite ABC Kit, Vector Laboratories Inc., Burlingame, CA, USA; 3.2 ml/ml) in phosphate buffered saline (PBS)-Tx100 (0.5%/4% NaCl) for 1 h, washed three times in PB, moved to a solution of 0.0001% biotin-tyramide (IRIS Biotech GmbH, Marktredwitz, Germany) and 0.003% H2 O2 in 0.05 M borate buffer (pH 8.5) for 1 h, washed again three times in PB and incubated in the same ABC-solution as before for 1 h. After washing in PB and acetate imidazole buffer (AIP, 0.175 M acetate, 0.069% imidazole, pH 7.4/6.5), tissue was pre-incubated in a 0.025% diaminobenzidine solution (DAB-buffer tablets for microscopy, Merck KGaA, Darmstadt, Germany) with 1% NiSO4 in AIP (pH 6.5) for 5 min and the chromogenic reaction was induced by adding H2 O2 (end concentration 0.0025%) for 3 min. After washing the tissue, the sections were mounted on gelatin-coated slides, counterstained using neutral red, and coverslipped using DPX (DPX Mountant for Histology, Sigma-Aldrich GmbH, Steinheim, Germany). .. The brains from in vivo tracing experiments were treated in the same way but were sliced in 50 μm sections and the TSA steps were omitted.

    Incubation:

    Article Title: Kainate receptors are primarily postsynaptic to SP-containing axon terminals in the trigeminal dorsal horn
    Article Snippet: .. Bound primary antibody was visualized by incubating tissue sections in biotinylated donkey α rat IgG (1:400; Vector Laboratories, Burlingame, CA) or biotinylated donkey α mouse IgM (1:400; Jackson ImmunoResearch, West Grove, PA) secondary antibody followed by incubation in Avidin-Biotin (Elite Vectastain ABC kit; Vector Laboratories) and diaminobenzidine-hydrogen peroxidase (DAB-H2 O2 ) solutions. ..

    Article Title: Use of c-fos to identify activity-dependent spinal neurons after stepping in intact adult rats
    Article Snippet: .. The sections were incubated at RT for 1 h in 1:200 Vectastain Elite Avidin-Biotin Peroxidase Complex (ABC) solution (Vector Laboratories, Burlingame, CA, USA), then rinsed again in PBS and placed in diaminobenzidine (DAB) solution (Sigma-Aldrich Corp., St Louis, MO, USA) for 15 min for the chromogen reaction to occur. ..

    Article Title: Functional recovery of stepping in rats after a complete neonatal spinal cord transection is not due to regrowth across the lesion site
    Article Snippet: .. To detect BDA-labeling, free-floating spinal cord sections were first incubated in 0.3% H2 O2 in phosphate buffered saline solution (PBS) (15 min), rinsed in PBS, and incubated in Vectastain Elite avidin-biotin-peroxidase (ABC) complex (1:200 in PBS for 1 h; Vector Labs, Burlingame, CA). .. Following ABC incubation, the sections were rinsed in PBS, incubated in diaminobenzidine (DAB) (SigmaFast DAB kit for 20 min; Sigma-Aldrich, St. Louis, MO), and stored in PBS.

    Article Title: Anatomy and Physiology of Neurons in Layer 9 of the Chicken Optic Tectum
    Article Snippet: .. The tissue was then incubated in avidin-biotin peroxidase complex (ABC; Vectastain Elite ABC Kit, Vector Laboratories Inc., Burlingame, CA, USA; 3.2 ml/ml) in phosphate buffered saline (PBS)-Tx100 (0.5%/4% NaCl) for 1 h, washed three times in PB, moved to a solution of 0.0001% biotin-tyramide (IRIS Biotech GmbH, Marktredwitz, Germany) and 0.003% H2 O2 in 0.05 M borate buffer (pH 8.5) for 1 h, washed again three times in PB and incubated in the same ABC-solution as before for 1 h. After washing in PB and acetate imidazole buffer (AIP, 0.175 M acetate, 0.069% imidazole, pH 7.4/6.5), tissue was pre-incubated in a 0.025% diaminobenzidine solution (DAB-buffer tablets for microscopy, Merck KGaA, Darmstadt, Germany) with 1% NiSO4 in AIP (pH 6.5) for 5 min and the chromogenic reaction was induced by adding H2 O2 (end concentration 0.0025%) for 3 min. After washing the tissue, the sections were mounted on gelatin-coated slides, counterstained using neutral red, and coverslipped using DPX (DPX Mountant for Histology, Sigma-Aldrich GmbH, Steinheim, Germany). .. The brains from in vivo tracing experiments were treated in the same way but were sliced in 50 μm sections and the TSA steps were omitted.

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation
    Article Snippet: .. Subsequently, the sections were blocked with the block solution (1–3% protein) for 2 h, incubated with primary antibodies (anti-SARS-CoV SUD and anti-mouse CD11b (ab133357; Abcam)) at 4 °C overnight, treated in 3% H2 O2 for 10 min to quench the endogenous peroxidase activity, and reacted with biotinylated universal antibodies from a VECTASTAIN® Elite® ABC Universal Kit (Vector Laboratories, Burlingame, CA, USA) for 30 min. After incubating with VECTASTAIN elite ABC reagent 30 min, the dark brown-black precipitate of the immune complexes in the sections was developed using DAB Peroxidase Substrate Kit (Vector Laboratories). ..

    Article Title: TDP-43 pathology disrupts nuclear pore complexes and nucleocytoplasmic transport in ALS/FTD
    Article Snippet: .. The following day sections were incubated in biotinylated secondary antibody at 5 μL/mL (Elite IgG Vectastain ABC kit) for 30 mins at 37°C and then incubated with the avidin-biotin enzyme complex (Vector Laboratories) for 30 mins. .. Stains were visualized by incubation of DAB Chromogen (Sigma-Aldrich) for 5 mins at RT.

    Activity Assay:

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation
    Article Snippet: .. Subsequently, the sections were blocked with the block solution (1–3% protein) for 2 h, incubated with primary antibodies (anti-SARS-CoV SUD and anti-mouse CD11b (ab133357; Abcam)) at 4 °C overnight, treated in 3% H2 O2 for 10 min to quench the endogenous peroxidase activity, and reacted with biotinylated universal antibodies from a VECTASTAIN® Elite® ABC Universal Kit (Vector Laboratories, Burlingame, CA, USA) for 30 min. After incubating with VECTASTAIN elite ABC reagent 30 min, the dark brown-black precipitate of the immune complexes in the sections was developed using DAB Peroxidase Substrate Kit (Vector Laboratories). ..

    Blocking Assay:

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation
    Article Snippet: .. Subsequently, the sections were blocked with the block solution (1–3% protein) for 2 h, incubated with primary antibodies (anti-SARS-CoV SUD and anti-mouse CD11b (ab133357; Abcam)) at 4 °C overnight, treated in 3% H2 O2 for 10 min to quench the endogenous peroxidase activity, and reacted with biotinylated universal antibodies from a VECTASTAIN® Elite® ABC Universal Kit (Vector Laboratories, Burlingame, CA, USA) for 30 min. After incubating with VECTASTAIN elite ABC reagent 30 min, the dark brown-black precipitate of the immune complexes in the sections was developed using DAB Peroxidase Substrate Kit (Vector Laboratories). ..

    Staining:

    Article Title: Immunohistochemical Markers for Quantitative Studies of Neurons and Glia in Human Neocortex
    Article Snippet: .. The background staining did not compromise results in tissue stored in 4% Lillies PBFS, but the signal level was lower than that of both the Envision+ peroxidase-labeled polymer detection system and the Vectastain Universal Elite ABC kit ( – ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Vector Laboratories vectastain elite abc
    Histopathological changes in the lung tissues of the wild type mice intratracheally instilled with pSUD-FL, pSUD-NM, and pSUD-MC. The sections of the lung tissues from the instilled mice were performed using immunohistochemistry staining with anti-SARS-CoV SUD (( A ), first and second rows), or anti-mouse CD11b (( A ), third and fourth rows), and biotinylated universal antibodies from a <t>VECTASTAIN</t> ® Elite ® <t>ABC</t> Universal Kit ( A ). The sections were also assessed with H E staining, which the wide range of lung tissues was observed by a light microscope at 40× and 200× magnification (( B ), first and second rows); inflammatory cells infiltrated around the peribranchial and perivascular, and cell accumulation in alveolar space were examined using a light microscope at 400× magnification (( B ), third and fourth rows). The indices of histopathological changes in aggregation ( C ), perivascular cuffing ( D ), inflammation ( E ), and pathological index ( F ) were scored based on the degree of lesions ranged from one to five depending on severity: 1 = minimal (
    Vectastain Elite Abc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain elite abc/product/Vector Laboratories
    Average 96 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    vectastain elite abc - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Histopathological changes in the lung tissues of the wild type mice intratracheally instilled with pSUD-FL, pSUD-NM, and pSUD-MC. The sections of the lung tissues from the instilled mice were performed using immunohistochemistry staining with anti-SARS-CoV SUD (( A ), first and second rows), or anti-mouse CD11b (( A ), third and fourth rows), and biotinylated universal antibodies from a VECTASTAIN ® Elite ® ABC Universal Kit ( A ). The sections were also assessed with H E staining, which the wide range of lung tissues was observed by a light microscope at 40× and 200× magnification (( B ), first and second rows); inflammatory cells infiltrated around the peribranchial and perivascular, and cell accumulation in alveolar space were examined using a light microscope at 400× magnification (( B ), third and fourth rows). The indices of histopathological changes in aggregation ( C ), perivascular cuffing ( D ), inflammation ( E ), and pathological index ( F ) were scored based on the degree of lesions ranged from one to five depending on severity: 1 = minimal (

    Journal: International Journal of Molecular Sciences

    Article Title: SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation

    doi: 10.3390/ijms21093179

    Figure Lengend Snippet: Histopathological changes in the lung tissues of the wild type mice intratracheally instilled with pSUD-FL, pSUD-NM, and pSUD-MC. The sections of the lung tissues from the instilled mice were performed using immunohistochemistry staining with anti-SARS-CoV SUD (( A ), first and second rows), or anti-mouse CD11b (( A ), third and fourth rows), and biotinylated universal antibodies from a VECTASTAIN ® Elite ® ABC Universal Kit ( A ). The sections were also assessed with H E staining, which the wide range of lung tissues was observed by a light microscope at 40× and 200× magnification (( B ), first and second rows); inflammatory cells infiltrated around the peribranchial and perivascular, and cell accumulation in alveolar space were examined using a light microscope at 400× magnification (( B ), third and fourth rows). The indices of histopathological changes in aggregation ( C ), perivascular cuffing ( D ), inflammation ( E ), and pathological index ( F ) were scored based on the degree of lesions ranged from one to five depending on severity: 1 = minimal (

    Article Snippet: Subsequently, the sections were blocked with the block solution (1–3% protein) for 2 h, incubated with primary antibodies (anti-SARS-CoV SUD and anti-mouse CD11b (ab133357; Abcam)) at 4 °C overnight, treated in 3% H2 O2 for 10 min to quench the endogenous peroxidase activity, and reacted with biotinylated universal antibodies from a VECTASTAIN® Elite® ABC Universal Kit (Vector Laboratories, Burlingame, CA, USA) for 30 min. After incubating with VECTASTAIN elite ABC reagent 30 min, the dark brown-black precipitate of the immune complexes in the sections was developed using DAB Peroxidase Substrate Kit (Vector Laboratories).

    Techniques: Mouse Assay, Immunohistochemistry, Staining, Light Microscopy