succinylation  (Vector Laboratories)


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    ImmPRESS HRP Anti Mouse IgG Rat adsorbed Peroxidase Polymer Detection Kit made in Horse
    Description:
    ImmPRESS HRP Polymer Kit is a polymerized reporter enzyme staining system that uses novel conjugation and micropolymer chemistries to create a highly sensitive ready to use one step detection system This unique micropolymer of highly active enzyme peroxidase or alkaline phosphatase is attached to our affinity purified secondary antibodies producing reagents with outstanding sensitivity and low background The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones The result is crisp strong staining of antibody targets especially nuclear and membrane antigens such as Ki67 estrogen receptor bcl 2 CD3 CD8 and CD10 and greater sensitivity than other polymer systems Key advantages of the ImmPRESS Polymer System High sensitivityLow backgroundMicropolymer technologyEnhanced accessibility to nuclear and membrane antigensOne step detectionReady to use in a convenient dropper bottleShorter assay timeSimplified multiple labeling
    Catalog Number:
    mp-7422
    Price:
    None
    Size:
    15 ml
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    Mouse
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    Structured Review

    Vector Laboratories succinylation
    ImmPRESS HRP Anti Mouse IgG Rat adsorbed Peroxidase Polymer Detection Kit made in Horse
    ImmPRESS HRP Polymer Kit is a polymerized reporter enzyme staining system that uses novel conjugation and micropolymer chemistries to create a highly sensitive ready to use one step detection system This unique micropolymer of highly active enzyme peroxidase or alkaline phosphatase is attached to our affinity purified secondary antibodies producing reagents with outstanding sensitivity and low background The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones The result is crisp strong staining of antibody targets especially nuclear and membrane antigens such as Ki67 estrogen receptor bcl 2 CD3 CD8 and CD10 and greater sensitivity than other polymer systems Key advantages of the ImmPRESS Polymer System High sensitivityLow backgroundMicropolymer technologyEnhanced accessibility to nuclear and membrane antigensOne step detectionReady to use in a convenient dropper bottleShorter assay timeSimplified multiple labeling
    https://www.bioz.com/result/succinylation/product/Vector Laboratories
    Average 88 stars, based on 785 article reviews
    Price from $9.99 to $1999.99
    succinylation - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism"

    Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

    Journal: Journal of proteome research

    doi: 10.1021/acs.jproteome.8b00800

    Pathway analysis of acetylated and succinylated mitochondrial proteins common to WT, SIRT3 KO and SIRT5 KO mice. (A) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with decreased succinylation. (B) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with increased acetylation. (C) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with increased acetylation and decreased succinylation at the same lysine residue as a function of chronic ethanol feeding (differential acyl switching proteins). (D) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with increased acetylation and/or decreased succinylation on different lysine residues as a function of chronic ethanol feeding. * The first number after each bar on the graph represents the number of acetylated and/or succinylated proteins identified per pathway. The second number represents the fold enrichment for that pathway.
    Figure Legend Snippet: Pathway analysis of acetylated and succinylated mitochondrial proteins common to WT, SIRT3 KO and SIRT5 KO mice. (A) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with decreased succinylation. (B) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with increased acetylation. (C) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with increased acetylation and decreased succinylation at the same lysine residue as a function of chronic ethanol feeding (differential acyl switching proteins). (D) Pathway analysis of all WT, SIRT3 KO and SIRT5 KO mouse liver mitochondrial proteins with increased acetylation and/or decreased succinylation on different lysine residues as a function of chronic ethanol feeding. * The first number after each bar on the graph represents the number of acetylated and/or succinylated proteins identified per pathway. The second number represents the fold enrichment for that pathway.

    Techniques Used: Mouse Assay

    Overlap of acetylated and succinylated mitochondrial peptides and proteins in all genotypes. Venn diagrams showing the overlap of acetylation and succinylation on peptides and proteins for all genotypes.
    Figure Legend Snippet: Overlap of acetylated and succinylated mitochondrial peptides and proteins in all genotypes. Venn diagrams showing the overlap of acetylation and succinylation on peptides and proteins for all genotypes.

    Techniques Used:

    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p
    Figure Legend Snippet: Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p

    Techniques Used: Western Blot

    Label-free acyl-quantitation of differential acyl switching peptides on lysine residues at or near regulatory sites. Acetylation and succinylation relative quantitation of (A) aspartate aminotransferase K404 from amino acid metabolism, (B) ornithine carbamoyltranferase K88 from the urea cycle, (C) malate dehydrogenase K301 from the TCA cycle, (D) hyroxyacyl-coenzyme A dehydrogenase from fatty acid oxidation, (E) superoxide dismutase 2 K68, and (F) K122 from redox pathways and glutathione metabolism (mean ± SEM, n=3, *=p
    Figure Legend Snippet: Label-free acyl-quantitation of differential acyl switching peptides on lysine residues at or near regulatory sites. Acetylation and succinylation relative quantitation of (A) aspartate aminotransferase K404 from amino acid metabolism, (B) ornithine carbamoyltranferase K88 from the urea cycle, (C) malate dehydrogenase K301 from the TCA cycle, (D) hyroxyacyl-coenzyme A dehydrogenase from fatty acid oxidation, (E) superoxide dismutase 2 K68, and (F) K122 from redox pathways and glutathione metabolism (mean ± SEM, n=3, *=p

    Techniques Used: Quantitation Assay

    Related Articles

    Staining:

    Article Title: Exploratory study on the effect of osteoactivin on muscle regeneration in a rat volumetric muscle loss model
    Article Snippet: .. F1.652 and CD68 staining was visualized with ImmPRESS™ HRP rat adsorbed anti-mouse IgG polymer detection kit (Vector Labs) and ImmPACT DAB Peroxidase substrate (Vector Labs). .. After rinsing in PBS for 5 min, F1.652 and CD68 sections were submerged in hematoxylin for 1 minute (for visualization of cell nuclei) and then mounted.

    Article Title: Involvement of DHH and GLI1 in adrenocortical autograft regeneration in rats
    Article Snippet: .. After washing with PBS containing 0.3% triton X-100, sections were incubated with ImmPRESS REAGENT anti-mouse IgG (MP-7422; VECTOR LABORATORIES, INC., CA, USA) for 30 min, followed by staining with diaminobenzidine. ..

    Incubation:

    Article Title: Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis
    Article Snippet: .. Then, sections were incubated with anti-rabbit (Vector laboratory, MP-7401), -mouse (Vector laboratory, MP-7422) or -goat (Vector laboratory, MP-7405) IgG secondary antibody reagent containing micropolymers coupled with either active peroxidase (ImmPRESS) or anti-guinea pig biotinylated secondary antibody (Vectastain ABC kit; Vector laboratory, PK-4007) for 1 h at room temperature. .. Staining for peroxidase was performed with 0.0125% 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Wako Pure Chemical Industries, 34900903) and 0.003% H2 O2 in 0.05 M Tris-HCl buffer (pH 7.6) for 5 min. Digital Images of immunostained sections were exposed using a standard light microscope (BX50 Olympus, Tokyo, Japan).

    Article Title: Involvement of DHH and GLI1 in adrenocortical autograft regeneration in rats
    Article Snippet: .. After washing with PBS containing 0.3% triton X-100, sections were incubated with ImmPRESS REAGENT anti-mouse IgG (MP-7422; VECTOR LABORATORIES, INC., CA, USA) for 30 min, followed by staining with diaminobenzidine. ..

    Article Title: Renal and colonic potassium transporters in the pregnant rat
    Article Snippet: .. Sections were washed in PBS; incubated for 30 min with polymer-linked, peroxidase-conjugated goat anti-mouse IgG (ImmPRESS MP-7422; Vector Laboratories, Burlingame, CA); washed again with PBS; and then exposed to diaminobenzidine (Vector DAB substrate kit SK-4100) for 5 min. .. Sections were washed in distilled water, dehydrated with xylene, mounted, and observed by light microscopy.

    other:

    Article Title: Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in Normal and Injured Rat Liver
    Article Snippet: Anti-mouse peroxidase–conjugated antibody was from Vector Laboratories (Immpress Reagent Kit; MP-7422; Burlingame, CA).

    Plasmid Preparation:

    Article Title: Renal and colonic potassium transporters in the pregnant rat
    Article Snippet: .. Sections were washed in PBS; incubated for 30 min with polymer-linked, peroxidase-conjugated goat anti-mouse IgG (ImmPRESS MP-7422; Vector Laboratories, Burlingame, CA); washed again with PBS; and then exposed to diaminobenzidine (Vector DAB substrate kit SK-4100) for 5 min. .. Sections were washed in distilled water, dehydrated with xylene, mounted, and observed by light microscopy.

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    Vector Laboratories vectastain abc hrp kit peroxidase guinea pig igg
    Vectastain Abc Hrp Kit Peroxidase Guinea Pig Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 84/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain abc hrp kit peroxidase guinea pig igg/product/Vector Laboratories
    Average 84 stars, based on 249 article reviews
    Price from $9.99 to $1999.99
    vectastain abc hrp kit peroxidase guinea pig igg - by Bioz Stars, 2020-09
    84/100 stars
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