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pinfuse migg2b fc2 vector  (InvivoGen)


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    Structured Review

    InvivoGen pinfuse migg2b fc2 vector
    Pinfuse Migg2b Fc2 Vector, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pinfuse+vector/pINFUSE-mIgG2B-Fc2/pm41110474-478-44-46
    Average 93 stars, based on 9 article reviews
    pinfuse migg2b fc2 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: BTNL2 promotes colitis-associated tumorigenesis in mice by regulating IL-22 production.
    Article Snippet: Interleukin 22 (IL-22) has an important role in colorectal tumorigenesis and many colorectal diseases such as inflammatory bowel disease and certain infections.. However, the regulation of IL-22 production in the intestinal system is still unclear.. Here, we present evidence that butyrophilin-like protein 2 (BTNL2) is required for colorectal IL-22 production, and BTNL2 knockout mice show decreased colonic tumorigenesis and more severe colitis phenotypes than control mice due to defective production of IL-22.

    Recombinant:

    Article Title: Phenotypic and functional characterization of CD4-2 + T cells in large yellow croaker Larimichthys crocea.
    Article Snippet: CD4 T cells, also known as helper T (Th) cells, are crucial regulators of the adaptive immune system, orchestrating and coordinating immune responses that protect the host against a broad spectrum of pathogenic threats.. Two CD4 co-receptor genes, cd4-1 and cd4-2, have been identified in teleost species, including the large yellow croaker Larimichthys crocea.. In this study, we developed a monoclonal antibody (mAb) that specifically recognizes croaker CD4-2, enabling the identification and characterization of CD4-2 T lymphocytes.

    Article Title: BTNL2 promotes colitis-associated tumorigenesis in mice by regulating IL-22 production.
    Article Snippet: Interleukin 22 (IL-22) has an important role in colorectal tumorigenesis and many colorectal diseases such as inflammatory bowel disease and certain infections.. However, the regulation of IL-22 production in the intestinal system is still unclear.. Here, we present evidence that butyrophilin-like protein 2 (BTNL2) is required for colorectal IL-22 production, and BTNL2 knockout mice show decreased colonic tumorigenesis and more severe colitis phenotypes than control mice due to defective production of IL-22.

    Clone Assay:

    Article Title: Phenotypic and functional characterization of CD4-2 + T cells in large yellow croaker Larimichthys crocea.
    Article Snippet: CD4 T cells, also known as helper T (Th) cells, are crucial regulators of the adaptive immune system, orchestrating and coordinating immune responses that protect the host against a broad spectrum of pathogenic threats.. Two CD4 co-receptor genes, cd4-1 and cd4-2, have been identified in teleost species, including the large yellow croaker Larimichthys crocea.. In this study, we developed a monoclonal antibody (mAb) that specifically recognizes croaker CD4-2, enabling the identification and characterization of CD4-2 T lymphocytes.

    Article Title: Novel single-domain antibodies targeting a unique transferrin receptor 1 epitope for cross-species delivery of drugs in the central nervous system.
    Article Snippet: The blood-brain barrier (BBB) is a major obstacle for delivering therapeutic agents to the central nervous system (CNS), posing significant challenges for treating neurological disorders.. Among current strategies to improve brain drug exposure, hijacking physiological pathways involved in receptor-mediated transcytosis has emerged as a promising strategy.. While targeting transferrin receptor 1 (TfR1) is widely explored, many TfR1antibodies lack cross-species reactivity, limiting translational development.

    Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier
    Article Snippet: .. Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains. ..

    Sequencing:

    Article Title: Phenotypic and functional characterization of CD4-2 + T cells in large yellow croaker Larimichthys crocea.
    Article Snippet: CD4 T cells, also known as helper T (Th) cells, are crucial regulators of the adaptive immune system, orchestrating and coordinating immune responses that protect the host against a broad spectrum of pathogenic threats.. Two CD4 co-receptor genes, cd4-1 and cd4-2, have been identified in teleost species, including the large yellow croaker Larimichthys crocea.. In this study, we developed a monoclonal antibody (mAb) that specifically recognizes croaker CD4-2, enabling the identification and characterization of CD4-2 T lymphocytes.

    Article Title: BTNL2 promotes colitis-associated tumorigenesis in mice by regulating IL-22 production.
    Article Snippet: Interleukin 22 (IL-22) has an important role in colorectal tumorigenesis and many colorectal diseases such as inflammatory bowel disease and certain infections.. However, the regulation of IL-22 production in the intestinal system is still unclear.. Here, we present evidence that butyrophilin-like protein 2 (BTNL2) is required for colorectal IL-22 production, and BTNL2 knockout mice show decreased colonic tumorigenesis and more severe colitis phenotypes than control mice due to defective production of IL-22.

    Article Title: m 6 A‐Dependent ITIH1 Regulated by TGF‐β Acts as a Target for Hepatocellular Carcinoma Progression
    Article Snippet: .. The cDNA sequence encoding the ITIH1 protein was PCR amplified and subcloned into the pINFUSE‐hIgG2‐Fc2 vector (InvivoGen, cat. no. pfc2‐hgin2) with a human IgG2Fc tag. .. The ITIH1‐Fc expression vector was transiently transfected into 293T cells by using FectoPRO transfection reagent from PolyPlus according to the manufacturer's instructions (cat. no. 116‐040).

    Plasmid Preparation:

    Article Title: Phenotypic and functional characterization of CD4-2 + T cells in large yellow croaker Larimichthys crocea.
    Article Snippet: CD4 T cells, also known as helper T (Th) cells, are crucial regulators of the adaptive immune system, orchestrating and coordinating immune responses that protect the host against a broad spectrum of pathogenic threats.. Two CD4 co-receptor genes, cd4-1 and cd4-2, have been identified in teleost species, including the large yellow croaker Larimichthys crocea.. In this study, we developed a monoclonal antibody (mAb) that specifically recognizes croaker CD4-2, enabling the identification and characterization of CD4-2 T lymphocytes.

    Article Title: Novel single-domain antibodies targeting a unique transferrin receptor 1 epitope for cross-species delivery of drugs in the central nervous system.
    Article Snippet: The blood-brain barrier (BBB) is a major obstacle for delivering therapeutic agents to the central nervous system (CNS), posing significant challenges for treating neurological disorders.. Among current strategies to improve brain drug exposure, hijacking physiological pathways involved in receptor-mediated transcytosis has emerged as a promising strategy.. While targeting transferrin receptor 1 (TfR1) is widely explored, many TfR1antibodies lack cross-species reactivity, limiting translational development.

    Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier
    Article Snippet: .. Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains. ..

    other:

    Article Title: m 6 A-Dependent ITIH1 Regulated by TGF-β Acts as a Target for Hepatocellular Carcinoma Progression.
    Article Snippet: Preparation of ITIH1-Fc Recombinant Protein: The cDNA sequence encoding the ITIH1 protein was PCR amplified and subcloned into the pINFUSE-hIgG2-Fc2 vector (InvivoGen, cat. no. pfc2-hgin2) with a human IgG2Fc tag.

    Polymerase Chain Reaction:

    Article Title: BTNL2 promotes colitis-associated tumorigenesis in mice by regulating IL-22 production.
    Article Snippet: Interleukin 22 (IL-22) has an important role in colorectal tumorigenesis and many colorectal diseases such as inflammatory bowel disease and certain infections.. However, the regulation of IL-22 production in the intestinal system is still unclear.. Here, we present evidence that butyrophilin-like protein 2 (BTNL2) is required for colorectal IL-22 production, and BTNL2 knockout mice show decreased colonic tumorigenesis and more severe colitis phenotypes than control mice due to defective production of IL-22.

    Article Title: m 6 A‐Dependent ITIH1 Regulated by TGF‐β Acts as a Target for Hepatocellular Carcinoma Progression
    Article Snippet: .. The cDNA sequence encoding the ITIH1 protein was PCR amplified and subcloned into the pINFUSE‐hIgG2‐Fc2 vector (InvivoGen, cat. no. pfc2‐hgin2) with a human IgG2Fc tag. .. The ITIH1‐Fc expression vector was transiently transfected into 293T cells by using FectoPRO transfection reagent from PolyPlus according to the manufacturer's instructions (cat. no. 116‐040).

    Amplification:

    Article Title: BTNL2 promotes colitis-associated tumorigenesis in mice by regulating IL-22 production.
    Article Snippet: Interleukin 22 (IL-22) has an important role in colorectal tumorigenesis and many colorectal diseases such as inflammatory bowel disease and certain infections.. However, the regulation of IL-22 production in the intestinal system is still unclear.. Here, we present evidence that butyrophilin-like protein 2 (BTNL2) is required for colorectal IL-22 production, and BTNL2 knockout mice show decreased colonic tumorigenesis and more severe colitis phenotypes than control mice due to defective production of IL-22.

    Article Title: m 6 A‐Dependent ITIH1 Regulated by TGF‐β Acts as a Target for Hepatocellular Carcinoma Progression
    Article Snippet: .. The cDNA sequence encoding the ITIH1 protein was PCR amplified and subcloned into the pINFUSE‐hIgG2‐Fc2 vector (InvivoGen, cat. no. pfc2‐hgin2) with a human IgG2Fc tag. .. The ITIH1‐Fc expression vector was transiently transfected into 293T cells by using FectoPRO transfection reagent from PolyPlus according to the manufacturer's instructions (cat. no. 116‐040).

    Cloning:

    Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier
    Article Snippet: .. Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains. ..



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    Production of bivalent HuscFv-Fcγ fusion proteins (fusion antibodies). ( A ) Amplicons of huscfvs-huIgG1 fc amplified from recombinant pINFUSE-Fc2 transformed JM109 E. coli clones by direct colony PCR (arrowhead). Lane M, 1 kb DNA ladder; N, negative control (no template); lanes 1–3, huscfvC2-huIgG1 fc ; lanes 4–6, huscfvC41-huIgG1 fc amplicons. ( B ) Diagram of the bivalent HuscFv-huIgG1 Fc (fusion antibody). ( C ) SDS-PAGE and CBB-stained fusion antibodies (bivalent HuscFvC2-IgG1 Fc) in various purification fractions. Fusion antibodies were purified from the culture supernatant of huscfv-huIgG1 Fc2 pINFUSE-transfected HEK293E suspension cells by protein G affinity column chromatography. Lane M, protein standard markers; lane 1, transfected HEK293E cell culture supernatant; lane 2, flow through; lanes 3–9, eluted fractions containing the fusion antibodies. ( D , E ) Fusion antibodies were subjected to SDS-PAGE under non-reducing conditions and reducing conditions, respectively. Non-reduced fusion antibodies appear as bands at ~110 kDa (arrowhead, bivalent HuscFvs-IgG1 Fc); reduced fusion antibodies appear as ~55 kDa bands (arrowhead, HuscFv-IgG1 Fc). Lane M, protein standard markers; lane 1, monovalent HuscFvC2 fusion antibody; lane 2, monovalent HuscFvC41 fusion antibody. Numbers on the left of ( C – E ) are the molecular masses of proteins in kDa.

    Journal: Vaccines

    Article Title: Agonistic Bivalent Human scFvs-Fcγ Fusion Antibodies to OX40 Ectodomain Enhance T Cell Activities against Cancer

    doi: 10.3390/vaccines11121826

    Figure Lengend Snippet: Production of bivalent HuscFv-Fcγ fusion proteins (fusion antibodies). ( A ) Amplicons of huscfvs-huIgG1 fc amplified from recombinant pINFUSE-Fc2 transformed JM109 E. coli clones by direct colony PCR (arrowhead). Lane M, 1 kb DNA ladder; N, negative control (no template); lanes 1–3, huscfvC2-huIgG1 fc ; lanes 4–6, huscfvC41-huIgG1 fc amplicons. ( B ) Diagram of the bivalent HuscFv-huIgG1 Fc (fusion antibody). ( C ) SDS-PAGE and CBB-stained fusion antibodies (bivalent HuscFvC2-IgG1 Fc) in various purification fractions. Fusion antibodies were purified from the culture supernatant of huscfv-huIgG1 Fc2 pINFUSE-transfected HEK293E suspension cells by protein G affinity column chromatography. Lane M, protein standard markers; lane 1, transfected HEK293E cell culture supernatant; lane 2, flow through; lanes 3–9, eluted fractions containing the fusion antibodies. ( D , E ) Fusion antibodies were subjected to SDS-PAGE under non-reducing conditions and reducing conditions, respectively. Non-reduced fusion antibodies appear as bands at ~110 kDa (arrowhead, bivalent HuscFvs-IgG1 Fc); reduced fusion antibodies appear as ~55 kDa bands (arrowhead, HuscFv-IgG1 Fc). Lane M, protein standard markers; lane 1, monovalent HuscFvC2 fusion antibody; lane 2, monovalent HuscFvC41 fusion antibody. Numbers on the left of ( C – E ) are the molecular masses of proteins in kDa.

    Article Snippet: For the cell transfection, the huscfv-huIgG1 Fc2 -pINFUSE vector was mixed with a transfection reagent (FectoPro, PolyPlus transfection, Illkirch, France).

    Techniques: Amplification, Recombinant, Transformation Assay, Clone Assay, Negative Control, SDS Page, Staining, Purification, Transfection, Suspension, Affinity Column, Chromatography, Cell Culture