Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Multiplex Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Amplification

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Intra Assay, Inter Assay