picopure rna isolation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher picopure rna isolation kit
    Picopure Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picopure rna isolation kit/product/Thermo Fisher
    Average 99 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    picopure rna isolation kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: PGCs were sorted into 100 μl of extraction buffer of PicoPure RNA Isolation Kit (Applied Biosystems) and RNA was extracted following the manufacturer’s protocol. .. The Ovation RNA-Seq System V2 (Nugen) was used for reverse transcription and amplification of total RNA (0.5 to 2 ng) into cDNA.

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. After total RNA extraction and DNaseI treatment, the RNA quality was assessed with the Experion automated electrophoresis system (Bio-Rad). cDNA was generated from these RNA samples with a Superscript III kit (Invitrogen) and amplified following a previously described protocol ( ; ).

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: .. Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen). .. RT-qPCR reactions were set up with SYBR Green JumpStart Taq ReadyMix (Sigma) and 200 nM of forward and reverse primers ( ).

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended. .. RNA amplification and sequencing was performed on all Arc samples by Cofactor Genomics (Saint Louis, MO).

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions. .. The double strand cDNA was subjected to end repair, A base addition, adaptor ligation and PCR amplification to create libraries.

    Synthesized:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: .. Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen). .. RT-qPCR reactions were set up with SYBR Green JumpStart Taq ReadyMix (Sigma) and 200 nM of forward and reverse primers ( ).

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: .. In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen). .. PCR reactions were carried out by using the PowerUp SYBR Green Master Mix (Applied Biosystems) on the QuantStudio3 Real-Time PCR system (Applied Biosystems), with primers listed in .

    Cytometry:

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: Paragraph title: Cell staining prior to sorting and flow cytometry ... Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation.

    Blocking Assay:

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: Prior to incubation with antibodies, cells were incubated in a blocking buffer (10% mouse serum and 1% human IgG in PBS) for 15 min at room temperature. .. Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation.

    Real-time Polymerase Chain Reaction:

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. The qualities of the final products were determined via qPCR with primers for gapdh , damb , and staufen .

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen). .. RT-qPCR was then performed on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) at 95°C for 10 minutes, 40 cycles of 95°C for 15 seconds and 60°C for 1 minute, followed by a melt curve stage.

    Article Title: Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
    Article Snippet: Paragraph title: Reverse Transcription and Quantitative PCR ... Pools of embryos were treated with 0.1% (w/v) proteinase from Streptomyces griseus to remove the zona pelluicida, washed three times in 50 µl droplets of DPBS with 1% (w/v) PVP, placed in 100 µl extraction buffer from the PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA, USA) and heated at 42°C for 30 min. Total RNA was extracted using the PicoPure RNA isolation kit (Applied Biosystems), treated with 2 U of DNase (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min to remove DNA, and then incubated at 75°C for 15 min to denature DNase.

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: Paragraph title: Gene expression analysis by quantitative real time PCR (qRT-PCR) ... In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen).

    Article Title: Free fatty acid receptors, G protein‐coupled receptor 120 and G protein‐coupled receptor 40, are essential for oil‐induced gastric inhibitory polypeptide secretion
    Article Snippet: .. Ribonucleic acid isolation and real‐time polymerase chain reaction A total of 2000 GFP‐positive cells (K cells) were collected into extraction buffer of the Picopure RNA isolation kit (Applied Biosystems, Alameda, CA, USA). .. Total ribonucleic acid (RNA) was extracted from the cells and was treated with deoxyribonuclease (Qiagen, Valencia, CA, USA).

    Multiplex Assay:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: PGCs were sorted into 100 μl of extraction buffer of PicoPure RNA Isolation Kit (Applied Biosystems) and RNA was extracted following the manufacturer’s protocol. .. Subsequently, using Ovation Rapid DR Multiplex System (Nugen) and 500 ng of fragmented cDNA an RNASeq library was prepared.

    Incubation:

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: Prior to incubation with antibodies, cells were incubated in a blocking buffer (10% mouse serum and 1% human IgG in PBS) for 15 min at room temperature. .. Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation.

    Article Title: Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
    Article Snippet: .. Pools of embryos were treated with 0.1% (w/v) proteinase from Streptomyces griseus to remove the zona pelluicida, washed three times in 50 µl droplets of DPBS with 1% (w/v) PVP, placed in 100 µl extraction buffer from the PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA, USA) and heated at 42°C for 30 min. Total RNA was extracted using the PicoPure RNA isolation kit (Applied Biosystems), treated with 2 U of DNase (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min to remove DNA, and then incubated at 75°C for 15 min to denature DNase. .. DNase-treated RNA was then reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Expressing:

    Article Title: Characterisation of Anopheles strains used for laboratory screening of new vector control products
    Article Snippet: .. Quantification of resistance-associated gene expression RNA was extracted from three pools of 5–7, 2–5-day-old females using a PicoPure RNA isolation kit (Thermo Fisher Scientific, Warrington, UK). .. One to four μg of RNA from each biological replicate was reverse transcribed using Oligo dT (Invitrogen, Warrington, UK) and Superscript III (Invitrogen).

    Article Title: Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
    Article Snippet: Reverse Transcription and Quantitative PCR Analysis of gene expression was accomplished by quantitative reverse transcription PCR. .. Pools of embryos were treated with 0.1% (w/v) proteinase from Streptomyces griseus to remove the zona pelluicida, washed three times in 50 µl droplets of DPBS with 1% (w/v) PVP, placed in 100 µl extraction buffer from the PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA, USA) and heated at 42°C for 30 min. Total RNA was extracted using the PicoPure RNA isolation kit (Applied Biosystems), treated with 2 U of DNase (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min to remove DNA, and then incubated at 75°C for 15 min to denature DNase.

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: Paragraph title: Gene expression analysis by quantitative real time PCR (qRT-PCR) ... In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen).

    Ex Vivo:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: Sorted cells were either left untouched (ex-vivo ) or stimulated with LPS+anti-IgM for 6h in IMDM media. .. Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions.

    Flow Cytometry:

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: Paragraph title: Cell staining prior to sorting and flow cytometry ... Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation.

    Sequencing:

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended. .. RNA amplification and sequencing was performed on all Arc samples by Cofactor Genomics (Saint Louis, MO).

    Article Title: Free fatty acid receptors, G protein‐coupled receptor 120 and G protein‐coupled receptor 40, are essential for oil‐induced gastric inhibitory polypeptide secretion
    Article Snippet: Ribonucleic acid isolation and real‐time polymerase chain reaction A total of 2000 GFP‐positive cells (K cells) were collected into extraction buffer of the Picopure RNA isolation kit (Applied Biosystems, Alameda, CA, USA). .. The quantitative polymerase chain reaction was carried out using ABI PRISM 7000 Sequence Detection System (Applied Biosystems).

    Ligation:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions. .. The double strand cDNA was subjected to end repair, A base addition, adaptor ligation and PCR amplification to create libraries.

    Dissection:

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: Female flies that expressed green fluorescent protein (GFP) in KCs with OK107-Gal4 were maintained at 25°C before dissection. .. The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification.

    Generated:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: PGCs were sorted into 100 μl of extraction buffer of PicoPure RNA Isolation Kit (Applied Biosystems) and RNA was extracted following the manufacturer’s protocol. .. Fragments of 250 bp were generated by sonication of amplified cDNA using the Covaris S220 Focused-ultrasonicators.

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. After total RNA extraction and DNaseI treatment, the RNA quality was assessed with the Experion automated electrophoresis system (Bio-Rad). cDNA was generated from these RNA samples with a Superscript III kit (Invitrogen) and amplified following a previously described protocol ( ; ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: In Hfe / Nrf2 −/− experiment, RNA was extracted using TriZol, DNase-treated, and reverse transcribed to cDNA (ThermoScript RT-PCR System). .. In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen).

    Sonication:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: PGCs were sorted into 100 μl of extraction buffer of PicoPure RNA Isolation Kit (Applied Biosystems) and RNA was extracted following the manufacturer’s protocol. .. Fragments of 250 bp were generated by sonication of amplified cDNA using the Covaris S220 Focused-ultrasonicators.

    Injection:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: RNA-seq Splenic CD19+ CD21hi CD24hi B cells were isolated from Mb1 cre and Ahr fl/- Mb1 cre mice in the remission phase of arthritis, at day 7 post-IA injection. .. Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions.

    RNA Sequencing Assay:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: Paragraph title: RNA-Seq Library Preparation ... PGCs were sorted into 100 μl of extraction buffer of PicoPure RNA Isolation Kit (Applied Biosystems) and RNA was extracted following the manufacturer’s protocol.

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: Paragraph title: cDNA preparation for RNA-seq. ... The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification.

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: .. Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen). .. RT-qPCR reactions were set up with SYBR Green JumpStart Taq ReadyMix (Sigma) and 200 nM of forward and reverse primers ( ).

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: Paragraph title: Laser-capture microdissection and RNA sequencing ... Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended.

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: Paragraph title: RNA-seq ... Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions.

    Isolation:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: .. PGCs were sorted into 100 μl of extraction buffer of PicoPure RNA Isolation Kit (Applied Biosystems) and RNA was extracted following the manufacturer’s protocol. .. The Ovation RNA-Seq System V2 (Nugen) was used for reverse transcription and amplification of total RNA (0.5 to 2 ng) into cDNA.

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: .. The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. After total RNA extraction and DNaseI treatment, the RNA quality was assessed with the Experion automated electrophoresis system (Bio-Rad). cDNA was generated from these RNA samples with a Superscript III kit (Invitrogen) and amplified following a previously described protocol ( ; ).

    Article Title: AhR-mediated Gene Expression in the Developing Mouse Telencephalon
    Article Snippet: .. The PicoPure™ RNA Isolation Kit, Arcturus # KIT0202 / KIT0204 was used to lyse samples and isolate RNA from dorsal and ventral telencephalon samples following manufacturer's instructions. .. The quality of RNA samples was verified using the PicoChip for Agilent BioAnalyzer.

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: .. Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation. ..

    Article Title: Characterisation of Anopheles strains used for laboratory screening of new vector control products
    Article Snippet: .. Quantification of resistance-associated gene expression RNA was extracted from three pools of 5–7, 2–5-day-old females using a PicoPure RNA isolation kit (Thermo Fisher Scientific, Warrington, UK). .. One to four μg of RNA from each biological replicate was reverse transcribed using Oligo dT (Invitrogen, Warrington, UK) and Superscript III (Invitrogen).

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: .. Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen). .. RT-qPCR reactions were set up with SYBR Green JumpStart Taq ReadyMix (Sigma) and 200 nM of forward and reverse primers ( ).

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: .. RNA extraction was performed per the Arcturus PicoPure® RNA Isolation Kit protocol (Thermo Fisher Scientific; KIT0204). .. RNA yield was quantified with a NanoDrop 2000 Spectrophotometer.

    Article Title: Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
    Article Snippet: .. Pools of embryos were treated with 0.1% (w/v) proteinase from Streptomyces griseus to remove the zona pelluicida, washed three times in 50 µl droplets of DPBS with 1% (w/v) PVP, placed in 100 µl extraction buffer from the PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA, USA) and heated at 42°C for 30 min. Total RNA was extracted using the PicoPure RNA isolation kit (Applied Biosystems), treated with 2 U of DNase (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min to remove DNA, and then incubated at 75°C for 15 min to denature DNase. .. DNase-treated RNA was then reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: .. Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended. .. RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies, Waldbronn, Germany).

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: .. In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen). .. PCR reactions were carried out by using the PowerUp SYBR Green Master Mix (Applied Biosystems) on the QuantStudio3 Real-Time PCR system (Applied Biosystems), with primers listed in .

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: .. Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions. .. 60bp single reads were sequenced on 3 lanes of an Illumina hiseq.

    Article Title: Free fatty acid receptors, G protein‐coupled receptor 120 and G protein‐coupled receptor 40, are essential for oil‐induced gastric inhibitory polypeptide secretion
    Article Snippet: .. Ribonucleic acid isolation and real‐time polymerase chain reaction A total of 2000 GFP‐positive cells (K cells) were collected into extraction buffer of the Picopure RNA isolation kit (Applied Biosystems, Alameda, CA, USA). .. Total ribonucleic acid (RNA) was extracted from the cells and was treated with deoxyribonuclease (Qiagen, Valencia, CA, USA).

    Article Title: Prenatal Exposure to Environmentally-Relevant Contaminants Perturbs Male Reproductive Parameters Across Multiple Generations that are Partially Protected by Folic Acid Supplementation
    Article Snippet: .. From each pool, total RNA was extracted using the PicoPure™ RNA Isolation Kit (ThermoFisher Scientific, Mississauga, ON, Canada). .. Two-cell embryo RNA sequencing cDNA libraries were constructed using SMART-Seq v4 Ultra low input RNA Kit (Clontech Laboratories, Takara Bio Company; CA, USA).

    Transferring:

    Article Title: Prenatal Exposure to Environmentally-Relevant Contaminants Perturbs Male Reproductive Parameters Across Multiple Generations that are Partially Protected by Folic Acid Supplementation
    Article Snippet: With a 100 µM Cook® Flexipet® Pipette (Cook Medical LLC, Bloomington, IN, USA), two-cell embryos were collected and stored at −80 °C until all embryos were obtained. .. From each pool, total RNA was extracted using the PicoPure™ RNA Isolation Kit (ThermoFisher Scientific, Mississauga, ON, Canada).

    Microscopy:

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: The flies were dissected under a fluorescent microscope in ice-cold PBS–BSA solution to isolate the MBs with tweezers. .. The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification.

    Purification:

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: .. The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. After total RNA extraction and DNaseI treatment, the RNA quality was assessed with the Experion automated electrophoresis system (Bio-Rad). cDNA was generated from these RNA samples with a Superscript III kit (Invitrogen) and amplified following a previously described protocol ( ; ).

    Polymerase Chain Reaction:

    Article Title: Characterisation of Anopheles strains used for laboratory screening of new vector control products
    Article Snippet: Quantification of resistance-associated gene expression RNA was extracted from three pools of 5–7, 2–5-day-old females using a PicoPure RNA isolation kit (Thermo Fisher Scientific, Warrington, UK). .. The resulting cDNA was diluted to 4 ng/µl and used as a template in the subsequent PCR reactions.

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: Paragraph title: Quantitative Reverse Transcription PCR (RT-qPCR) ... Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen).

    Article Title: Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
    Article Snippet: Reverse Transcription and Quantitative PCR Analysis of gene expression was accomplished by quantitative reverse transcription PCR. .. Pools of embryos were treated with 0.1% (w/v) proteinase from Streptomyces griseus to remove the zona pelluicida, washed three times in 50 µl droplets of DPBS with 1% (w/v) PVP, placed in 100 µl extraction buffer from the PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA, USA) and heated at 42°C for 30 min. Total RNA was extracted using the PicoPure RNA isolation kit (Applied Biosystems), treated with 2 U of DNase (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min to remove DNA, and then incubated at 75°C for 15 min to denature DNase.

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen). .. PCR reactions were carried out by using the PowerUp SYBR Green Master Mix (Applied Biosystems) on the QuantStudio3 Real-Time PCR system (Applied Biosystems), with primers listed in .

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions. .. The double strand cDNA was subjected to end repair, A base addition, adaptor ligation and PCR amplification to create libraries.

    Quantitative RT-PCR:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: Paragraph title: Quantitative Reverse Transcription PCR (RT-qPCR) ... Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen).

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: Paragraph title: Gene expression analysis by quantitative real time PCR (qRT-PCR) ... In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen).

    Staining:

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: Paragraph title: Cell staining prior to sorting and flow cytometry ... Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation.

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: For LCM, sections were allowed to thaw for 5 min at 4 °C and then stained for 5 min in a 0.1% cresyl violet acetate (Sigma-Aldrich, St. Louis, MI) solution dissolved in 70% ethanol. .. Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended.

    Mouse Assay:

    Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
    Article Snippet: RNA-seq Splenic CD19+ CD21hi CD24hi B cells were isolated from Mb1 cre and Ahr fl/- Mb1 cre mice in the remission phase of arthritis, at day 7 post-IA injection. .. Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions.

    SYBR Green Assay:

    Article Title: Segregation of mitochondrial DNA heteroplasmy through a development genetic bottleneck in human embryos
    Article Snippet: Total RNA from sorted PGCs or somatic cells was extracted by PicoPure RNA Isolation Kit (Applied Biosystems). cDNA was synthesized and amplified using Ovation RNA-Seq System V2 (Nugen). .. RT-qPCR reactions were set up with SYBR Green JumpStart Taq ReadyMix (Sigma) and 200 nM of forward and reverse primers ( ).

    Article Title: Nrf2 controls iron homeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Article Snippet: All reactions were performed with iQ SYBR Green Supermix, with primers listed in Suplementary Table 3. .. In LSECs experiments, total RNA was isolated using PicoPure RNA Isolation Kit (Thermo Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen).

    RNA Extraction:

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. After total RNA extraction and DNaseI treatment, the RNA quality was assessed with the Experion automated electrophoresis system (Bio-Rad). cDNA was generated from these RNA samples with a Superscript III kit (Invitrogen) and amplified following a previously described protocol ( ; ).

    Article Title: Evidence that tissue resident human enthesis γδT-cells can produce IL-17A independently of IL-23R transcript expression
    Article Snippet: .. Cells were sorted using an Influx (BD) cell sorter directly into RNA extraction buffer supplied as a component of the PicoPure RNA isolation kit (Thermo Fisher), which was then used throughout for RNA isolation. ..

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: .. RNA extraction was performed per the Arcturus PicoPure® RNA Isolation Kit protocol (Thermo Fisher Scientific; KIT0204). .. RNA yield was quantified with a NanoDrop 2000 Spectrophotometer.

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: .. Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended. .. RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies, Waldbronn, Germany).

    Electrophoresis:

    Article Title: Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
    Article Snippet: The isolated MBs were immediately lysed with XB buffer (PicoPure RNA isolation Kit; Thermo Fisher Scientific) at 42°C for 30 min and were subsequently maintained at −80°C until RNA purification. .. After total RNA extraction and DNaseI treatment, the RNA quality was assessed with the Experion automated electrophoresis system (Bio-Rad). cDNA was generated from these RNA samples with a Superscript III kit (Invitrogen) and amplified following a previously described protocol ( ; ).

    Laser Capture Microdissection:

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: Paragraph title: Laser-capture microdissection and RNA sequencing ... Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended.

    Random Hexamer Labeling:

    Article Title: Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
    Article Snippet: Pools of embryos were treated with 0.1% (w/v) proteinase from Streptomyces griseus to remove the zona pelluicida, washed three times in 50 µl droplets of DPBS with 1% (w/v) PVP, placed in 100 µl extraction buffer from the PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA, USA) and heated at 42°C for 30 min. Total RNA was extracted using the PicoPure RNA isolation kit (Applied Biosystems), treated with 2 U of DNase (New England Biolabs, Ipswich, MA, USA) at 37°C for 30 min to remove DNA, and then incubated at 75°C for 15 min to denature DNase. .. Reverse transcription occurred using random hexamer primers and involved incubation at 25°C for 10 min, 37°C for 120 min and 85°C for 5 min.

    Spectrophotometry:

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: RNA extraction was performed per the Arcturus PicoPure® RNA Isolation Kit protocol (Thermo Fisher Scientific; KIT0204). .. RNA yield was quantified with a NanoDrop 2000 Spectrophotometer.

    Lysis:

    Article Title: Global transcriptome analysis of rat hypothalamic arcuate nucleus demonstrates reversal of hypothalamic gliosis following surgically and diet induced weight loss
    Article Snippet: .. Lysis of cells and RNA extraction was performed using the PicoPure RNA isolation kit (Life Technologies, Carlsbad, CA) as recommended. .. RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies, Waldbronn, Germany).

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  • 90
    Thermo Fisher picopure rna isolation kit
    Picopure Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picopure rna isolation kit/product/Thermo Fisher
    Average 90 stars, based on 39 article reviews
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    picopure rna isolation kit - by Bioz Stars, 2020-01
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