picopure rna isolation kit  (Thermo Fisher)


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    Name:
    PicoPure RNA Isolation Kit
    Description:
    The ARCTURUS PicoPure RNA Isolation Kit is designed to recover high quality total RNA consistently from fewer than ten cells even from a single cell This kit is designed to provide consistent efficient RNA recovery without sacrificing quality Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis Key Product Features • Greater sensitivty isolate RNA from even a single cell • Superior efficiency maximize RNA recovery from samples • Streamlined concentration elute with small volumes • Flexible application compatible with many tube sizes • Integrated contamination prevention column lids ensure that samples remain RNase free Isolate RNA from Even a Single CellThe PicoPure RNA Isolation Kit is engineered to recover high quality total RNA consistently from fewer than ten cells The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100 µg of RNA Since the RNA elutes in as little as 10 µl of buffer it is ready for gene expression analysis without any vacuum concentration This kit is available in two sizes enough for 40 or 200 isolations Get Superior RNA RecoverySmall valuable samples often cannot be replaced Performing gene expression studies on these precious samples requires getting maximum RNA recovery Comparative studies show that with small RNA poor cell or tissue samples the PicoPure Kit dramatically outperforms other commercial total RNA isolation kits in efficiency Eliminate Time consuming RNA ConcentrationThe simple isolation protocol consists of extracting cellular RNA then loading the extract onto the specially designed MiraCol Purification Column to bind the RNA After washing away impurities the RNA elutes in only 10 µl of buffer ready for use without vacuum concentration or transfer into a new reaction tube High Yield Purification ColumnsThe PicoPure RNA Isolation Kit features unique high recovery MiraCol Purification Columns engineered to maximize recovery from microscopic samples The columns have convenient integrated lids to help ensure that samples remain RNase free In addition the Kit comes complete with all necessary buffers an easy to follow User Guide optimized protocols for handling Laser Capture Microdissected LCM samples and protocols for isolating RNA from larger samples Deliver RNA Ready for Amplification and Microarray AnalysisRNA isolated with the PicoPure RNA Kit is ready without vacuum concentration for amplification with the RiboAmp RNA Amplification Kit The two kits together allow for highly reproducible gene expression results Retain Low abundance mRNAThe PicoPure RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process minimizing the loss of important genes of interest Low medium and high abundance genes are consistently recovered and available for expression analysis Maintain High RNA QualityThe PicoPure RNA isolation process allows for high quality RNA Total cellular RNA prepared from LCM samples remains intact after isolation increasing confidence in subsequent molecular analysis
    Catalog Number:
    kit0204
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|Laser Capture Microdissection|RNA Extraction|Total RNA Isolation|Total RNA from Animal Cells & Tissues|Gene Expression Analysis & Genotyping|LCM Kits & Reagents
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher picopure rna isolation kit
    Comparison of <t>RNA</t> quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus <t>PicoPure</t> Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    The ARCTURUS PicoPure RNA Isolation Kit is designed to recover high quality total RNA consistently from fewer than ten cells even from a single cell This kit is designed to provide consistent efficient RNA recovery without sacrificing quality Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis Key Product Features • Greater sensitivty isolate RNA from even a single cell • Superior efficiency maximize RNA recovery from samples • Streamlined concentration elute with small volumes • Flexible application compatible with many tube sizes • Integrated contamination prevention column lids ensure that samples remain RNase free Isolate RNA from Even a Single CellThe PicoPure RNA Isolation Kit is engineered to recover high quality total RNA consistently from fewer than ten cells The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100 µg of RNA Since the RNA elutes in as little as 10 µl of buffer it is ready for gene expression analysis without any vacuum concentration This kit is available in two sizes enough for 40 or 200 isolations Get Superior RNA RecoverySmall valuable samples often cannot be replaced Performing gene expression studies on these precious samples requires getting maximum RNA recovery Comparative studies show that with small RNA poor cell or tissue samples the PicoPure Kit dramatically outperforms other commercial total RNA isolation kits in efficiency Eliminate Time consuming RNA ConcentrationThe simple isolation protocol consists of extracting cellular RNA then loading the extract onto the specially designed MiraCol Purification Column to bind the RNA After washing away impurities the RNA elutes in only 10 µl of buffer ready for use without vacuum concentration or transfer into a new reaction tube High Yield Purification ColumnsThe PicoPure RNA Isolation Kit features unique high recovery MiraCol Purification Columns engineered to maximize recovery from microscopic samples The columns have convenient integrated lids to help ensure that samples remain RNase free In addition the Kit comes complete with all necessary buffers an easy to follow User Guide optimized protocols for handling Laser Capture Microdissected LCM samples and protocols for isolating RNA from larger samples Deliver RNA Ready for Amplification and Microarray AnalysisRNA isolated with the PicoPure RNA Kit is ready without vacuum concentration for amplification with the RiboAmp RNA Amplification Kit The two kits together allow for highly reproducible gene expression results Retain Low abundance mRNAThe PicoPure RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process minimizing the loss of important genes of interest Low medium and high abundance genes are consistently recovered and available for expression analysis Maintain High RNA QualityThe PicoPure RNA isolation process allows for high quality RNA Total cellular RNA prepared from LCM samples remains intact after isolation increasing confidence in subsequent molecular analysis
    https://www.bioz.com/result/picopure rna isolation kit/product/Thermo Fisher
    Average 99 stars, based on 155 article reviews
    Price from $9.99 to $1999.99
    picopure rna isolation kit - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq"

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2017.00185

    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Figure Legend Snippet: Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Techniques Used: Laser Capture Microdissection, Isolation

    2) Product Images from "Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq"

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2017.00185

    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Figure Legend Snippet: Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Techniques Used: Laser Capture Microdissection, Isolation

    Related Articles

    In Vivo:

    Article Title: Significance and Suppression of Redundant IL17 Responses in Acute Allograft Rejection by Bioinformatics Based Drug Repositioning of Fenofibrate
    Article Snippet: .. Human in vitro and murine in vivo QPCR For quantification of gene expression in in-vitro and in-vivo experiments by quantitative PCR (QPCR), total RNA from human PBMC was isolated using the Pico Pure RNA isolation Kit (Arcturus, Life Technologies, Foster City, CA). .. Total RNA from mice allograft and spleen tissues was extracted using TRIzol® Reagent (Invitrogen, Life Technologies, Carlsbad, CA) .

    Amplification:

    Article Title: Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing
    Article Snippet: .. RNA extraction, amplification and cDNA preparation Total RNAs of papilla cells collected by LM were extracted using the Pico-Pure RNA isolation kit (Applied Biosystems, Life Technologies). .. The quantity and quality of total RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Pico kit (Agilent Technologies).

    In Vitro:

    Article Title: Significance and Suppression of Redundant IL17 Responses in Acute Allograft Rejection by Bioinformatics Based Drug Repositioning of Fenofibrate
    Article Snippet: .. Human in vitro and murine in vivo QPCR For quantification of gene expression in in-vitro and in-vivo experiments by quantitative PCR (QPCR), total RNA from human PBMC was isolated using the Pico Pure RNA isolation Kit (Arcturus, Life Technologies, Foster City, CA). .. Total RNA from mice allograft and spleen tissues was extracted using TRIzol® Reagent (Invitrogen, Life Technologies, Carlsbad, CA) .

    Isolation:

    Article Title: Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
    Article Snippet: .. RNA extraction and measurement The following RNA extraction kits were used to isolate total RNA from LCM acquired material: RNeasy® Micro (Qiagen, Germany), miRNeasy Mini (Qiagen, Germany), Arcturus® Picopure® RNA isolation kit (Arcturus, Applied Biosystems, USA.), mirVana™ miRNA isolation kit (Ambion, USA.) and RNAqueous® -Micro (Ambion, USA.). .. Samples were taken from a −80 °C freezer and lysis buffer from the according RNA extraction kit was immediately added to the sample at room temperature.

    Article Title: Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing
    Article Snippet: .. RNA extraction, amplification and cDNA preparation Total RNAs of papilla cells collected by LM were extracted using the Pico-Pure RNA isolation kit (Applied Biosystems, Life Technologies). .. The quantity and quality of total RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Pico kit (Agilent Technologies).

    Article Title: KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system
    Article Snippet: .. RNA extraction and RT–qPCR As previously described , total RNA was extracted using the Pico‐Pure RNA Isolation Kit (Thermo, USA) according to the manufacturer's instructions. .. Total RNA was extracted from each pool of embryos (n = 3 pools of 20 oocytes or embryos per time point), and residual genomic DNA was removed by DNase digestion, using an RNase‐Free DNase Kit (Qiagen, Germany).

    Article Title: Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida
    Article Snippet: .. To aspirate cell content, the capillary needle (TransferTip® —R ICIS, inner diameter 4 µM, outer diameter 7 µM, tip angle 35°, Eppendorf, Hamburg, Germany) of the micro manipulator (CellTram® vario, Eppendorf, Hamburg, Germany) filled with RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit, Life Technologies, USA) was used to pierce the plant root with assistance from TransferMan® (Eppendorf, Hamburg, Germany). .. While entering the root, the CellTram® vario was turned to the injection mode to avoid the entry of any unwanted plant cell material into the needle due to the turgor pressure of the plant cell.

    Article Title: Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida
    Article Snippet: .. The microaspirate contents were immediately transferred to 10 µL RNA extraction buffer provided in the ARCTURUS PicoPure RNA Isolation Kit (Life Technologies, USA), frozen in liquid nitrogen, and stored at −80 °C. .. Cell content of uninfected cells from the infected roots were also aspirated for gene expression comparison.

    Article Title: Significance and Suppression of Redundant IL17 Responses in Acute Allograft Rejection by Bioinformatics Based Drug Repositioning of Fenofibrate
    Article Snippet: .. Human in vitro and murine in vivo QPCR For quantification of gene expression in in-vitro and in-vivo experiments by quantitative PCR (QPCR), total RNA from human PBMC was isolated using the Pico Pure RNA isolation Kit (Arcturus, Life Technologies, Foster City, CA). .. Total RNA from mice allograft and spleen tissues was extracted using TRIzol® Reagent (Invitrogen, Life Technologies, Carlsbad, CA) .

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: .. The lysates were loaded onto the spin column and total RNA purification was performed by following PicoPure® RNA isolation kit user guide (Thermo Fisher Scientific, Cat #KIT0204), including on-column DNase-treatment (QIAGEN, Cat#79254). ..

    Laser Capture Microdissection:

    Article Title: Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
    Article Snippet: .. RNA extraction and measurement The following RNA extraction kits were used to isolate total RNA from LCM acquired material: RNeasy® Micro (Qiagen, Germany), miRNeasy Mini (Qiagen, Germany), Arcturus® Picopure® RNA isolation kit (Arcturus, Applied Biosystems, USA.), mirVana™ miRNA isolation kit (Ambion, USA.) and RNAqueous® -Micro (Ambion, USA.). .. Samples were taken from a −80 °C freezer and lysis buffer from the according RNA extraction kit was immediately added to the sample at room temperature.

    RNA Extraction:

    Article Title: Immunofluorescence laser micro-dissection of specific nephron segments in the mouse kidney allows targeted downstream proteomic analysis
    Article Snippet: .. RNA extraction and real-time PCR RNA was extracted using Pico-pure RNA kit (Life Technologies # 12204-01). .. An additional concentration step was performed using standard isopropanol precipitation, before reverse transcription and real-time PCR.

    Article Title: Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
    Article Snippet: .. RNA extraction and measurement The following RNA extraction kits were used to isolate total RNA from LCM acquired material: RNeasy® Micro (Qiagen, Germany), miRNeasy Mini (Qiagen, Germany), Arcturus® Picopure® RNA isolation kit (Arcturus, Applied Biosystems, USA.), mirVana™ miRNA isolation kit (Ambion, USA.) and RNAqueous® -Micro (Ambion, USA.). .. Samples were taken from a −80 °C freezer and lysis buffer from the according RNA extraction kit was immediately added to the sample at room temperature.

    Article Title: Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing
    Article Snippet: .. RNA extraction, amplification and cDNA preparation Total RNAs of papilla cells collected by LM were extracted using the Pico-Pure RNA isolation kit (Applied Biosystems, Life Technologies). .. The quantity and quality of total RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Pico kit (Agilent Technologies).

    Article Title: KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system
    Article Snippet: .. RNA extraction and RT–qPCR As previously described , total RNA was extracted using the Pico‐Pure RNA Isolation Kit (Thermo, USA) according to the manufacturer's instructions. .. Total RNA was extracted from each pool of embryos (n = 3 pools of 20 oocytes or embryos per time point), and residual genomic DNA was removed by DNase digestion, using an RNase‐Free DNase Kit (Qiagen, Germany).

    Article Title: Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida
    Article Snippet: .. To aspirate cell content, the capillary needle (TransferTip® —R ICIS, inner diameter 4 µM, outer diameter 7 µM, tip angle 35°, Eppendorf, Hamburg, Germany) of the micro manipulator (CellTram® vario, Eppendorf, Hamburg, Germany) filled with RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit, Life Technologies, USA) was used to pierce the plant root with assistance from TransferMan® (Eppendorf, Hamburg, Germany). .. While entering the root, the CellTram® vario was turned to the injection mode to avoid the entry of any unwanted plant cell material into the needle due to the turgor pressure of the plant cell.

    Article Title: Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida
    Article Snippet: .. The microaspirate contents were immediately transferred to 10 µL RNA extraction buffer provided in the ARCTURUS PicoPure RNA Isolation Kit (Life Technologies, USA), frozen in liquid nitrogen, and stored at −80 °C. .. Cell content of uninfected cells from the infected roots were also aspirated for gene expression comparison.

    Quantitative RT-PCR:

    Article Title: KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system
    Article Snippet: .. RNA extraction and RT–qPCR As previously described , total RNA was extracted using the Pico‐Pure RNA Isolation Kit (Thermo, USA) according to the manufacturer's instructions. .. Total RNA was extracted from each pool of embryos (n = 3 pools of 20 oocytes or embryos per time point), and residual genomic DNA was removed by DNase digestion, using an RNase‐Free DNase Kit (Qiagen, Germany).

    Purification:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: .. The lysates were loaded onto the spin column and total RNA purification was performed by following PicoPure® RNA isolation kit user guide (Thermo Fisher Scientific, Cat #KIT0204), including on-column DNase-treatment (QIAGEN, Cat#79254). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Immunofluorescence laser micro-dissection of specific nephron segments in the mouse kidney allows targeted downstream proteomic analysis
    Article Snippet: .. RNA extraction and real-time PCR RNA was extracted using Pico-pure RNA kit (Life Technologies # 12204-01). .. An additional concentration step was performed using standard isopropanol precipitation, before reverse transcription and real-time PCR.

    Article Title: Significance and Suppression of Redundant IL17 Responses in Acute Allograft Rejection by Bioinformatics Based Drug Repositioning of Fenofibrate
    Article Snippet: .. Human in vitro and murine in vivo QPCR For quantification of gene expression in in-vitro and in-vivo experiments by quantitative PCR (QPCR), total RNA from human PBMC was isolated using the Pico Pure RNA isolation Kit (Arcturus, Life Technologies, Foster City, CA). .. Total RNA from mice allograft and spleen tissues was extracted using TRIzol® Reagent (Invitrogen, Life Technologies, Carlsbad, CA) .

    Expressing:

    Article Title: Significance and Suppression of Redundant IL17 Responses in Acute Allograft Rejection by Bioinformatics Based Drug Repositioning of Fenofibrate
    Article Snippet: .. Human in vitro and murine in vivo QPCR For quantification of gene expression in in-vitro and in-vivo experiments by quantitative PCR (QPCR), total RNA from human PBMC was isolated using the Pico Pure RNA isolation Kit (Arcturus, Life Technologies, Foster City, CA). .. Total RNA from mice allograft and spleen tissues was extracted using TRIzol® Reagent (Invitrogen, Life Technologies, Carlsbad, CA) .

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: .. The lysates were loaded onto the spin column and total RNA purification was performed by following PicoPure® RNA isolation kit user guide (Thermo Fisher Scientific, Cat #KIT0204), including on-column DNase-treatment (QIAGEN, Cat79254). ..

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  • 99
    Thermo Fisher picopure rna isolation kit
    Comparison of <t>RNA</t> quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus <t>PicoPure</t> Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Picopure Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picopure rna isolation kit/product/Thermo Fisher
    Average 99 stars, based on 155 article reviews
    Price from $9.99 to $1999.99
    picopure rna isolation kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

    doi: 10.3389/fnmol.2017.00185

    Figure Lengend Snippet: Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Article Snippet: The lysates were loaded onto the spin column and total RNA purification was performed by following PicoPure® RNA isolation kit user guide (Thermo Fisher Scientific, Cat #KIT0204), including on-column DNase-treatment (QIAGEN, Cat#79254).

    Techniques: Laser Capture Microdissection, Isolation