pias3  (WuXi AppTec)

 
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  • 91
    Name:
    PIAS3 Antibody C term
    Description:
    Purified Rabbit Polyclonal Antibody Pab Rabbit Ig
    Catalog Number:
    ap1244a
    Domain:
    588-619
    Price:
    [109.0, 325.0]
    Applications:
    Western Blot,IHC (Paraffin)
    Host:
    Rabbit
    Immunogen:
    This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588-619 amino acids of human PIAS3.
    Size:
    80 ul 400 ul
    Category:
    Antibody
    Antibody Type:
    Primary antibody
    Isotype:
    Ig
    Preparation:
    This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588 619 amino acids of human PIAS3
    Reactivity:
    Human
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    Structured Review

    WuXi AppTec pias3
    <t>PIAS3</t> is an ATF1-binding protein
    Purified Rabbit Polyclonal Antibody Pab Rabbit Ig
    https://www.bioz.com/result/pias3/product/WuXi AppTec
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pias3 - by Bioz Stars, 2020-08
    91/100 stars

    Images

    1) Product Images from "PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element *PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element * s"

    Article Title: PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element *PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element * s

    Journal:

    doi: 10.1074/jbc.M701477200

    PIAS3 is an ATF1-binding protein
    Figure Legend Snippet: PIAS3 is an ATF1-binding protein

    Techniques Used: Binding Assay

    SUMO-E3 ligase domain of PIAS3 is not required for the override of ATF1-mediated ferritin H ARE repression
    Figure Legend Snippet: SUMO-E3 ligase domain of PIAS3 is not required for the override of ATF1-mediated ferritin H ARE repression

    Techniques Used:

    PIAS3 reverses ATF1-mediated repression of the human ferritin H transcription via the ARE
    Figure Legend Snippet: PIAS3 reverses ATF1-mediated repression of the human ferritin H transcription via the ARE

    Techniques Used:

    PIAS3 blocks ATF1 binding to the ARE in vivo
    Figure Legend Snippet: PIAS3 blocks ATF1 binding to the ARE in vivo

    Techniques Used: Binding Assay, In Vivo

    PIAS3 knockdown decreases basal expression and oxidative stress-mediated induction of ferritin H
    Figure Legend Snippet: PIAS3 knockdown decreases basal expression and oxidative stress-mediated induction of ferritin H

    Techniques Used: Expressing

    2) Product Images from "Developmental profiles of SUMOylation pathway proteins in rat cerebrum and cerebellum"

    Article Title: Developmental profiles of SUMOylation pathway proteins in rat cerebrum and cerebellum

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0212857

    Developmental profiles of SUMO E3 ligases and the deSUMOylating enzyme SENP3. Representative blots for PIAS1, PIAS3 and SENP3 and graphs showing the quantified levels of immunoreactivity from cerebrum and cerebellum homogenates at the designated age points. Graphs show the levels of immunoreactivity at different ages expressed as a percentage of the levels present in E18 brain. (n = 3, * = p≤0.05, ** = p≤0.01, *** = p≤0.001).
    Figure Legend Snippet: Developmental profiles of SUMO E3 ligases and the deSUMOylating enzyme SENP3. Representative blots for PIAS1, PIAS3 and SENP3 and graphs showing the quantified levels of immunoreactivity from cerebrum and cerebellum homogenates at the designated age points. Graphs show the levels of immunoreactivity at different ages expressed as a percentage of the levels present in E18 brain. (n = 3, * = p≤0.05, ** = p≤0.01, *** = p≤0.001).

    Techniques Used:

    3) Product Images from "Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia"

    Article Title: Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-12135-7

    The tumor suppressor genes PTEN, PDCD4 and PIAS3, targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.
    Figure Legend Snippet: The tumor suppressor genes PTEN, PDCD4 and PIAS3, targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.

    Techniques Used:

    Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. ( a ) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. ( b ) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. ( c – d ) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al ., Blood 2011 23 . mRNA expression level of miR-21 ( c ) and PTEN ( d ) were analyzed and compared by Wilcoxon matched pairs signed rank test. ( e ) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P
    Figure Legend Snippet: Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. ( a ) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. ( b ) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. ( c – d ) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al ., Blood 2011 23 . mRNA expression level of miR-21 ( c ) and PTEN ( d ) were analyzed and compared by Wilcoxon matched pairs signed rank test. ( e ) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P

    Techniques Used: Co-Culture Assay, Expressing, Cell Culture, Quantitative RT-PCR

    Related Articles

    Western Blot:

    Article Title: PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element *PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element * s
    Article Snippet: .. The cell lysates were subjected to Western blotting to detect PIAS3, ferritin H, and β-actin expression levels using anti-PIAS3 (AP1245a; Abgent), anti-ferritin H (sc-25617) and anti-β-actin (A5441; Sigma) antibodies. .. Total RNA was also isolated, and 10 μg of RNA was subjected to Northern blotting for hybridization with 32 P-labeled human ferritin H cDNA probe.

    Incubation:

    Article Title: MicroRNA-18a modulates STAT3 activity through negative regulation of PIAS3 during gastric adenocarcinogenesis
    Article Snippet: .. For detection of PIAS3, Survivin, or c-Myc, cells were incubated overnight at room temperature (RT) with PIAS3 antibody (rabbit anti-human PIAS3 polyclonal antibody (N-term) at 1 : 100 dilution; Abgent, San Diego, CA, USA), Survivin antibody (rabbit anti-human Survivin polyclonal antibody at 1 : 1000 dilution; Novus Biologicals, Littleton, CO, USA), or c-Myc antibody (mouse monoclonal [9E10] at 1 : 300 dilution; Abcam, Cambridge, UK), respectively. .. Samples were then washed three times in PBS and incubated with Dako EnVision Labelled Polymer Peroxidase secondary antibody for 30 min at RT.

    Article Title: Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia
    Article Snippet: .. Membranes were incubated overnight at 4 °C with primary antibodies against phospho-ZAP-70Tyr319 /SykTyr352 , phospho-AktSer473 , phospho-ERK1/2Thr202/Tyr204 , phospho-STAT3Ser727, Akt, ERK1/2, PTEN and PDCD4 (Cell signalling Technology Inc.), ZAP-70 (clone 2F3.2; Upstate Biotechnology), PIAS3 (WuXi AppTec.ABGENT) and β-actin (Abcam). .. Immunodetection was done with the corresponding IgG HRP-linked secondary antibodies (Dako North America) for 1 hour at RT.

    Expressing:

    Article Title: PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element *PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element * s
    Article Snippet: .. The cell lysates were subjected to Western blotting to detect PIAS3, ferritin H, and β-actin expression levels using anti-PIAS3 (AP1245a; Abgent), anti-ferritin H (sc-25617) and anti-β-actin (A5441; Sigma) antibodies. .. Total RNA was also isolated, and 10 μg of RNA was subjected to Northern blotting for hybridization with 32 P-labeled human ferritin H cDNA probe.

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  • 91
    WuXi AppTec pias3
    The tumor suppressor genes PTEN, PDCD4 and <t>PIAS3,</t> targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.
    Pias3, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pias3/product/WuXi AppTec
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pias3 - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    The tumor suppressor genes PTEN, PDCD4 and PIAS3, targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.

    Journal: Scientific Reports

    Article Title: Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia

    doi: 10.1038/s41598-017-12135-7

    Figure Lengend Snippet: The tumor suppressor genes PTEN, PDCD4 and PIAS3, targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.

    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against phospho-ZAP-70Tyr319 /SykTyr352 , phospho-AktSer473 , phospho-ERK1/2Thr202/Tyr204 , phospho-STAT3Ser727 , Akt, ERK1/2, PTEN and PDCD4 (Cell signalling Technology Inc.), ZAP-70 (clone 2F3.2; Upstate Biotechnology), PIAS3 (WuXi AppTec.ABGENT) and β-actin (Abcam).

    Techniques:

    Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. ( a ) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. ( b ) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. ( c – d ) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al ., Blood 2011 23 . mRNA expression level of miR-21 ( c ) and PTEN ( d ) were analyzed and compared by Wilcoxon matched pairs signed rank test. ( e ) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P

    Journal: Scientific Reports

    Article Title: Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia

    doi: 10.1038/s41598-017-12135-7

    Figure Lengend Snippet: Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. ( a ) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. ( b ) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. ( c – d ) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al ., Blood 2011 23 . mRNA expression level of miR-21 ( c ) and PTEN ( d ) were analyzed and compared by Wilcoxon matched pairs signed rank test. ( e ) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P

    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against phospho-ZAP-70Tyr319 /SykTyr352 , phospho-AktSer473 , phospho-ERK1/2Thr202/Tyr204 , phospho-STAT3Ser727 , Akt, ERK1/2, PTEN and PDCD4 (Cell signalling Technology Inc.), ZAP-70 (clone 2F3.2; Upstate Biotechnology), PIAS3 (WuXi AppTec.ABGENT) and β-actin (Abcam).

    Techniques: Co-Culture Assay, Expressing, Cell Culture, Quantitative RT-PCR