pi cdc2 tyr15  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pi cdc2 tyr15
    ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with <t>Cdc2</t> and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.
    Pi Cdc2 Tyr15, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi cdc2 tyr15/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi cdc2 tyr15 - by Bioz Stars, 2023-03
    97/100 stars

    Images

    1) Product Images from "The HDAC Inhibitor LBH589 Induces ERK-Dependent Prometaphase Arrest in Prostate Cancer via HDAC6 Inactivation and Down-Regulation"

    Article Title: The HDAC Inhibitor LBH589 Induces ERK-Dependent Prometaphase Arrest in Prostate Cancer via HDAC6 Inactivation and Down-Regulation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0073401

    ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with Cdc2 and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.
    Figure Legend Snippet: ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with Cdc2 and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.

    Techniques Used: Immunostaining, Staining, Flow Cytometry

    pi cdc2 tyr15  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc pi cdc2 tyr15
    ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with <t>Cdc2</t> and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.
    Pi Cdc2 Tyr15, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi cdc2 tyr15/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi cdc2 tyr15 - by Bioz Stars, 2023-03
    97/100 stars

    Images

    1) Product Images from "The HDAC Inhibitor LBH589 Induces ERK-Dependent Prometaphase Arrest in Prostate Cancer via HDAC6 Inactivation and Down-Regulation"

    Article Title: The HDAC Inhibitor LBH589 Induces ERK-Dependent Prometaphase Arrest in Prostate Cancer via HDAC6 Inactivation and Down-Regulation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0073401

    ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with Cdc2 and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.
    Figure Legend Snippet: ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with Cdc2 and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.

    Techniques Used: Immunostaining, Staining, Flow Cytometry

    pi cdc2 tyr15  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc pi cdc2 tyr15
    Pi Cdc2 Tyr15, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi cdc2 tyr15/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi cdc2 tyr15 - by Bioz Stars, 2023-03
    86/100 stars

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    Cell Signaling Technology Inc pi cdc2 tyr15
    ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with <t>Cdc2</t> and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.
    Pi Cdc2 Tyr15, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi cdc2 tyr15/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi cdc2 tyr15 - by Bioz Stars, 2023-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with Cdc2 and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.

    Journal: PLoS ONE

    Article Title: The HDAC Inhibitor LBH589 Induces ERK-Dependent Prometaphase Arrest in Prostate Cancer via HDAC6 Inactivation and Down-Regulation

    doi: 10.1371/journal.pone.0073401

    Figure Lengend Snippet: ( A – B ) Immuno-staining of LNCaP and PC-3. Both cells were treated 75 nM LBH589 for 24 h. Immuno-staining ( A ) with Cdc2 and Cdc25C antibodies and ( B ) of γ-tubulin (Green) and DAPI (Blue) in vehicle control (left) and LBH589 treatment (right) in LNCaP. ( C – D ) MEK inhibitor attenuated LBH589-induced prometaphase arrest in LNCaP. The cells were pre-treated 30 with UO126 for 30 minutes and sequentially treated with LBH589 for 24 h. ( C ) The cell cycles were analyzed by PI-staining and flow cytometry. ( D ) The metaphase cells were counted by DAPI staining presented as condensate chromatin.

    Article Snippet: The antibodies included HDAC1 (#2062), c-Raf (#9422), Akt (#9272), Pi-Akt (#4058), Pi-Cdc25C (Ser216) (#9528), Pi-c-Raf (S259) (# 9421), Pi-c-Raf (S338) (#9427), and Pi-Cdc2 (Tyr15) (#9111) purchased from Cell Signaling Technology (Beverly, MA, USA); HDAC6 (sc-28386), Pi-ERK(sc-7383), ERK (sc-94), 14-3-3ζ (sc-1019), and Cdc2 (sc-54) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Aurora A (#07-648), H3-Ac (#06-599), H4-Ac (#06-598), p21 (#05-345), and PP2A (#05-421) from Millipore (Lake Placid, NY, USA); GAPDH and actin (Sigma-Aldrich); Aurora B (#1788-1), Cdc25C (#1302-1), and PP1 (#1950-1) from Epitomics (Burlingame, CA); and HDAC3 (ab32369) from Abcam (Cambridge, UK).

    Techniques: Immunostaining, Staining, Flow Cytometry