# pi 3 p dic16 (Echelon Biosciences)

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Echelon Biosciences manufactures this product

## Structured Review

Pi 3 P Dic16, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/pi 3 p dic16/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Coxiella burnetii effector CvpE maintains biogenesis of Coxiella -containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes"

**Article Title: **Coxiella burnetii effector CvpE maintains biogenesis of Coxiella -containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes

**Journal: **Virulence

**doi: **10.1080/21505594.2024.2350893

**Figure Legend Snippet:**CvpE binds PI(3)P and perturbs PIKfyve activity on lysosomes.

**Techniques Used: **Activity Assay

# mouse anti pi 3 p antibody (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Mouse Anti Pi 3 P Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/mouse anti pi 3 p antibody/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "p37 regulates VCP/p97 shuttling and functions in the nucleus and cytosol"

**Article Title: **p37 regulates VCP/p97 shuttling and functions in the nucleus and cytosol

**Journal: **Science Advances

**doi: **10.1126/sciadv.adl6082

**Figure Legend Snippet:**( A ) LC3 puncta in control and p47 siRNA–treated HeLa cells, treated with 400 nM of BafA1 for 4 hours; n = 3, two-tailed paired Student’s t test. Data are means ± SEM. ( B ) HTTQ74-EGFP aggregates in control or ATG16 knockout (KO) HeLa cells, treated with p47 siRNA or control. The number of HTTQ74-EGFP aggregates represents the number of cells containing visible aggregates in cells positive for EGFP signal; n = 4, two-tailed paired Student’s t test. Data are means ± SEM. ( C ) LC3-II levels in control and p47 siRNA–treated HeLa cells, treated with 400 nM of BafA1 for 4 hours; n = 4, one-sample t test. Data are means ± SEM. ( D ) Protein levels in HeLa cells treated with control or p47 siRNA. ( E and F ) Control, p47 KO cells, or p47 KO cells expressing p47 wild-type (WT) were incubated in Earle’s balanced salt solution (EBSS) starvation media for 2 hours and stained for WIPI2 (E) or PI(3)P (F); n = 3 to 6, one-way ANOVA ( P = 0.0005) with post hoc Tukey test for (E), one-way ANOVA ( P = 0.0245) with post hoc Tuckey test for (F), data are means ± SEM. ( G ) HeLa cells expressing SRAI-LC3B were treated with control, p47, or ATG7 siRNA for 72 hours before treatment with 20 μM SMER28 for 24 hours, followed by fluorescence-activated cell sorting (FACS) analysis; n = 9, one-way ANOVA ( P = 0.0005) with post hoc Tukey test, data are means ± SEM. Scale bars, 10 μm. In cDNA transfection experiments, matched empty vectors were used as controls for overexpression constructs, and in all knockdown experiments, we used nontargeting control siRNAs. ns, not significant; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

**Techniques Used: **Two Tailed Test, Knock-Out, Expressing, Incubation, Staining, Fluorescence, FACS, Transfection, Over Expression, Construct

**Figure Legend Snippet:**( A ) Control, p37 KO, and p37 KO HeLa cells reconstituted with p37-FLAG were treated with 400 nM BafA1 for 4 hours, followed by immunostaining for LC3 and FLAG; n = 3, one-way ANOVA ( P = 0.004) with post hoc Tukey test. ( B ) iNeurons treated with lentiviral-delivered p37 shRNA (#81 or #83) for 4 days were treated with 400 nM BafA1 for 6 hours; n = 4, one-sample t test. ( C ) Control, p37 KO, and p37 KO with p37-FLAG–expressing HeLa cells were incubated in EBSS for 2 hours, followed by immunostaining for PI(3)P; n = 3 to 4, one-sample t test and two-tailed unpaired Student’s t test. ( D and E ) Endogenous ATG14L was immunoprecipitated from control and p37 KO HeLa cells (D) or control and p37-Clover–overexpressing HeLa cells (E); n = 4 to 5; one-sample t test. ( F to H ) Control or p37-FLAG–overexpressing cells were incubated in EBSS for 2 hours (F and G) and treated with 5 μM CB-5083 for 3 hours (G) or 400 nM BafA1 for 4 hours (H) where indicated, followed by immunostaining for PI(3)P (F), WIPI2 (G), or LC3 (H); n = 3, two-tailed unpaired Student’s t test. ( I ) HeLa cells expressing WT or SHP mutant p37-FLAG for 24 hours, followed by treatment with 400 nM BafA1 for 4 hours; n = 3 to 4, one-sample t test. ( J ) Control or Beclin-1 KO HeLa cells expressing p37-FLAG were treated with 400 nM BafA1 for 4 hours; n = 5, one-sample t test. Data are means ± SEM. Scale bars, 10 μm. In cDNA transfection experiments, matched empty vectors were used as controls for overexpression constructs, and in all knockdown experiments, we used nontargeting control siRNAs.

**Techniques Used: **Immunostaining, shRNA, Expressing, Incubation, Two Tailed Test, Immunoprecipitation, Mutagenesis, Transfection, Over Expression, Construct

# pi 3 p mass elisa kit (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Pi 3 P Mass Elisa Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/pi 3 p mass elisa kit/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# bodipy fl dic 6 pi 3 4 p 2 (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Bodipy Fl Dic 6 Pi 3 4 P 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/bodipy fl dic 6 pi 3 4 p 2/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Inositol Hexaphosphate as an Inhibitor and Potential Regulator of p47 phox Membrane Anchoring"

**Article Title: **Inositol Hexaphosphate as an Inhibitor and Potential Regulator of p47 phox Membrane Anchoring

**Journal: **Biochemistry

**doi: **10.1021/acs.biochem.4c00117

**Figure Legend Snippet:**IP6 binds to the PX domain of p47 phox as observed by NMR. (A) 1 H– 15 N-HSQC overlay of 1:0 and 1:2 molar ratios of p47 phox -PX:IP6. Insets depict a titration series including 1:0.25, 1:0.5, 1:2, and 1:4 molar ratios of PX domain:IP6. High-affinity binding is implicated through line-broadening of resonances. (B) Mapping of CSPs upon addition of 2× IP6 to the PX domain (PDB: 1GD5 ). Residues depicting 1 and 2 standard deviations of a 20% trimmed mean are in pink and purple, respectively. Residues with unobservable or line-broadened peaks are depicted in black. (C) Comparison of CSPs upon 2× C 4 –PI(3,4)P 2 (purple) or IP6 (orange). Residues with unobservable or line-broadened peaks are shown as red circles offset from the x -axis.

**Techniques Used: **Titration, Binding Assay, Comparison

**Figure Legend Snippet:**Fluorescence polarization study to assess IP6 as a competitive inhibitor. (A) Binding of tracer BODIPY-PI(3,4)P 2 to p47 phox -PX and associated fit for K d determination. (B) Displacement of BODIPY-PI(3,4)P 2 tracer from the PX domain upon addition of IP6 and associated fit. Error bars represent standard deviation of experimental replicates; those not visible are smaller than the data points.

**Techniques Used: **Fluorescence, Binding Assay, Standard Deviation

# pi 3 p (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Pi 3 P, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/pi 3 p/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# anti pi 3 4 p 2 (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Anti Pi 3 4 P 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti pi 3 4 p 2/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# dic8 pi 3 p p 3008 (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Dic8 Pi 3 P P 3008, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/dic8 pi 3 p p 3008/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Nuclear phosphoinositide signaling promotes YAP/TAZ-TEAD transcriptional activity in breast cancer"

**Article Title: **Nuclear phosphoinositide signaling promotes YAP/TAZ-TEAD transcriptional activity in breast cancer

**Journal: **The EMBO Journal

**doi: **10.1038/s44318-024-00085-6

**Figure Legend Snippet:**( A ) The intensity of immunoblots of Fig. was quantified using ImageJ and the graph shows the mean ± s.d. of n = 3 independent experiments. * P < 0.05; ** P < 0.01, and n.s.; not significant in Student’s t test. ( B , C ) 0.05 μM His 6 -tagged PIPKIα ( B ) and IPMK ( C ) were incubated with 0.2 μM diC8 PI(4)P or diC8 PI(4,5)P 2 , respectively, in the absence or presence of various concentrations of GST-YAP (0.001, 0.01, 0.1, 1.0, and 10.0 μM). The activities of the kinases were measured using the ADP-Glo assay (Promega). The graphs show the mean±s.d. of n = 3 independent experiments. YAP did not alter the activity of either kinase. * P < 0.05; ** P < 0.01, and n.s.; not significant in Student’s t test. ( D ) Summary of GST alone or GST-YAP bindings to PI, PI(4,5)P 2 , or PI(3,4,5)P 3 measured by MST. Raw data are available in Appendix Fig. S . ( E ) A schematic representation of the molecular structure Ac 3 2API and how it can become metabolically incorporated into phosphoinositides after removal of acetyl groups by esterase to produce azido- myo -inositol probe 2API. ( F ) The intensity of the immunoblots of Fig. was quantified using ImageJ and the graph shows the mean ± s.d. of n = 3 independent experiments. * P < 0.05; ** P < 0.01, and n.s.; not significant in Student’s t test. ( G ) Starved MDA-MB-231 cells were fed with Ac 3 2API for 24 h in the presence of 10% dialyzed serum. Cells were lysed and azide-tagged molecules were conjugated to biotin–alkyne through a click reaction. Endogenous c-Myc was immunoprecipitated and the associated complexes were analyzed by immunoblotting. Representative immunoblot images of n = 2 independent experiments are shown. ( H ) The clicked lysates were analyzed by anti-PI(4,5)P 2 or PI(3,4,5)P 3 antibodies. Biotinylated 2API was resolved by streptavidin. Many immunoblot bands overlapped with streptavidin signals. Representative immunoblot images of n = 3 independent experiments are shown. ( I , J ) Starved MDA-MB-231 cells were stimulated with 10% serum for 1 h. The images are z-stacks of PI(4,5)P 2 -YAP PLA ( E ) and PI(3,4,5)P 3 -YAP PLA ( F ) taken using a confocal microscope with each frame differing by 0.2 μm. DAPI was used to stain the nucleus. Representative images of n = 3 independent experiments are shown. Scale bar, 10 μm. ( K , L ) MDA-MB-231 cells grown in 10% serum were stimulated with 5 μM LPA for 90 min. Cells were fixed and the association of YAP with PI(4,5)P 2 ( G ) or PI(3,4,5)P 3 ( H ) was visualized by PLA. The images were obtained by widefield epifluorescence microscopy. The number of PLA puncta was counted from at least 10 cells and the graph shows the mean±s.d. of n = 3 independent experiments. DAPI staining was used to distinguish the nucleus from the cytoplasm. Treating the cells with LPA significantly increased the number of nuclear puncta. Scale bar, 10 μm. * P < 0.05; ** P < 0.01, and n.s; not significant in Student’s t test.

**Techniques Used: **Western Blot, Incubation, Glo Assay, Activity Assay, Metabolic Labelling, Immunoprecipitation, Microscopy, Staining, Epifluorescence Microscopy

# dic8 pi 3 4 p2 p 3408 (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Dic8 Pi 3 4 P2 P 3408, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/dic8 pi 3 4 p2 p 3408/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# dic8 pi 3 5 p 2 p 3508 (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Dic8 Pi 3 5 P 2 P 3508, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/dic8 pi 3 5 p 2 p 3508/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Nuclear phosphoinositide signaling promotes YAP/TAZ-TEAD transcriptional activity in breast cancer"

**Article Title: **Nuclear phosphoinositide signaling promotes YAP/TAZ-TEAD transcriptional activity in breast cancer

**Journal: **The EMBO Journal

**doi: **10.1038/s44318-024-00085-6

**Figure Legend Snippet:**( A ) The intensity of immunoblots of Fig. was quantified using ImageJ and the graph shows the mean ± s.d. of n = 3 independent experiments. * P < 0.05; ** P < 0.01, and n.s.; not significant in Student’s t test. ( B , C ) 0.05 μM His 6 -tagged PIPKIα ( B ) and IPMK ( C ) were incubated with 0.2 μM diC8 PI(4)P or diC8 PI(4,5)P 2 , respectively, in the absence or presence of various concentrations of GST-YAP (0.001, 0.01, 0.1, 1.0, and 10.0 μM). The activities of the kinases were measured using the ADP-Glo assay (Promega). The graphs show the mean±s.d. of n = 3 independent experiments. YAP did not alter the activity of either kinase. * P < 0.05; ** P < 0.01, and n.s.; not significant in Student’s t test. ( D ) Summary of GST alone or GST-YAP bindings to PI, PI(4,5)P 2 , or PI(3,4,5)P 3 measured by MST. Raw data are available in Appendix Fig. S . ( E ) A schematic representation of the molecular structure Ac 3 2API and how it can become metabolically incorporated into phosphoinositides after removal of acetyl groups by esterase to produce azido- myo -inositol probe 2API. ( F ) The intensity of the immunoblots of Fig. was quantified using ImageJ and the graph shows the mean ± s.d. of n = 3 independent experiments. * P < 0.05; ** P < 0.01, and n.s.; not significant in Student’s t test. ( G ) Starved MDA-MB-231 cells were fed with Ac 3 2API for 24 h in the presence of 10% dialyzed serum. Cells were lysed and azide-tagged molecules were conjugated to biotin–alkyne through a click reaction. Endogenous c-Myc was immunoprecipitated and the associated complexes were analyzed by immunoblotting. Representative immunoblot images of n = 2 independent experiments are shown. ( H ) The clicked lysates were analyzed by anti-PI(4,5)P 2 or PI(3,4,5)P 3 antibodies. Biotinylated 2API was resolved by streptavidin. Many immunoblot bands overlapped with streptavidin signals. Representative immunoblot images of n = 3 independent experiments are shown. ( I , J ) Starved MDA-MB-231 cells were stimulated with 10% serum for 1 h. The images are z-stacks of PI(4,5)P 2 -YAP PLA ( E ) and PI(3,4,5)P 3 -YAP PLA ( F ) taken using a confocal microscope with each frame differing by 0.2 μm. DAPI was used to stain the nucleus. Representative images of n = 3 independent experiments are shown. Scale bar, 10 μm. ( K , L ) MDA-MB-231 cells grown in 10% serum were stimulated with 5 μM LPA for 90 min. Cells were fixed and the association of YAP with PI(4,5)P 2 ( G ) or PI(3,4,5)P 3 ( H ) was visualized by PLA. The images were obtained by widefield epifluorescence microscopy. The number of PLA puncta was counted from at least 10 cells and the graph shows the mean±s.d. of n = 3 independent experiments. DAPI staining was used to distinguish the nucleus from the cytoplasm. Treating the cells with LPA significantly increased the number of nuclear puncta. Scale bar, 10 μm. * P < 0.05; ** P < 0.01, and n.s; not significant in Student’s t test.

**Techniques Used: **Western Blot, Incubation, Glo Assay, Activity Assay, Metabolic Labelling, Immunoprecipitation, Microscopy, Staining, Epifluorescence Microscopy

# dic8 pi 3 p p 3008 (Echelon Biosciences)

Echelon Biosciences is a verified supplier

Echelon Biosciences manufactures this product

## Structured Review

Dic8 Pi 3 P P 3008, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/dic8 pi 3 p p 3008/product/Echelon Biosciences

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99