phytophthora capsici strain pwb24 (ATCC)
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phytophthora capsici strain pwb24
Phytophthora Capsici Strain Pwb24, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phytophthora capsici strain pwb24/product/ATCC
Average 90 stars, based on 1 article reviews
Phytophthora Capsici Strain Pwb24, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phytophthora capsici strain pwb24/product/ATCC
Average 90 stars, based on 1 article reviews
phytophthora capsici strain pwb24 - by Bioz Stars,
2025-05
90/100 stars
Images
1) Product Images from "BABA and Phytophthora nicotianae Induce Resistance to Phytophthora capsici in Chile Pepper ( Capsicum annuum )"
Article Title: BABA and Phytophthora nicotianae Induce Resistance to Phytophthora capsici in Chile Pepper ( Capsicum annuum )
Journal: PLoS ONE
doi: 10.1371/journal.pone.0128327
Figure Legend Snippet: (A) Capsicum annuum plants were soil inoculated with 375,000 P . nicotianae (isolate NMT1: Genbank: HQ711620) zoospores per plant or 10,000 P . capsici (strain PWB24: ATCC MYA-2289) zoospores per plant and watered daily for two weeks. (B) Capsicum annuum plants were foliar inoculated with 100,000 P . nicotianae zoospores or mock inoculated with water and incubated in a humidity chamber for 5 days at 28°C. No symptom development was observed on the crown, roots, or foliage of P . nicotianae inoculated plants.
Techniques Used: Incubation
Figure Legend Snippet: (A) C . annuum leaves were inoculated with 2,000 P . nicotianae zoospores, 2.5 mM BABA or mock inoculated with water and incubated in a humidity chamber. After 48 hours, inoculated leaf tissue was excised and imaged using a stereofluorescent microscope. (B) C . annuum plants were soil drenched with P . nicotianae or P . capsici zoospore solutions (100,000 zoospores per plant), 2.5 mM BABA or water and photosynthetic rate was measured using a Licor 6400 portable photosynthesis system 3 days post inoculation. Standard deviation bars are shown.
Techniques Used: Incubation, Microscopy, Standard Deviation
Figure Legend Snippet: Plants were soil drenched with 100,000 P . nicotianae zoospores, 2.5 mM BABA, or water and foliar inoculated with 2,000 P . capsici zoospores per leaf, and plants were incubated in a humidity chamber at 28°C. for 48 h Plants were removed from humidity chamber and watered normally and grown under fluorescent light for an additional two weeks. (A) P . capsici caused systemic foliar blight and death in all untreated plants, whereas 50%- 83% of P . nicotianae or BABA treated plants did not develop disease symptoms outside of the inoculated leaves. (B) Histochemical x-gluc staining of GUS expressing P . capsici was utilized to visualize pathogen structures on inoculated C . annuum leaves. At 72 h post inoculation, abundant P . capsici hyphae and sporangia were visible on untreated plants, while BABA and P . nicotianae treated plants displayed confined areas of x-gluc staining with no identifiable P . capsici structures. Scale bar 30 μm for all images.
Techniques Used: Incubation, Staining, Expressing
Figure Legend Snippet: The hydrogen peroxide indicator stain 3-3- diaminobenzidine (DAB) was used to visualize reactive oxygen species production at 12, 24, and 48 h post P . capsici inoculation. The presence of hydrogen peroxide is indicated by the formation of a dark precipitate. P . capsici hyphae and germtubes are indicated with arrows. Tissue was imaged using a compound microscope with a digital camera mounted using an eyepiece adapter (Nikon, Melville, NY). Scale bar is 10 μm for all images.
Techniques Used: Staining, Microscopy
Figure Legend Snippet: Capsicum annuum seedlings were treated with BABA, P . nicotianae (P.nic), or water (H2O) and challenged with P . capsici (+) or mock inoculated with water (-) for a total of 6 treatments, with each treatment replicated on 4 blocks with 15 seedlings each in each block. Normalized peak area for each metabolite was log transformed and autoscaled to focus on relative changes across treatments. Substantial shifts in metabolite concentrations were identified for BABA treated plants, with no clear distinction between P . capsici (+) or mock challenged (-) groups. P . nicotianae (P.nic) induced plants that were challenged with P . capsici were distinguishable from non-induced (H2O) plants at the 95% confidence interval.
Techniques Used: Blocking Assay, Transformation Assay
Figure Legend Snippet: Capsicum annuum seedlings were treated with BABA, P . nicotianae (P.nic), or water (H2O) and challenged with P . capsici (+) or mock inoculated with water (-) for a total of 6 treatments, with each treatment replicated on 4 blocks with 15 seedlings each in each block. Metabolites in red indicate higher relative concentrations while those in blue indicate lower relative concentrations.
Techniques Used: Blocking Assay
Figure Legend Snippet: Capsicum annuum seedlings were treated with BABA, P . nicotianae (P.nic), or water (H2O) and challenged with P . capsici (+) or mock inoculated with water (-) for a total of 6 treatments, with each treatment replicated on 4 blocks with 15 seedlings each in each block. Dendrogram was constructed using the Spearman distance measure and Ward clustering algorithm. Metabolites in red indicate higher relative concentrations while those in blue indicate lower relative concentrations.
Techniques Used: Blocking Assay, Construct
Figure Legend Snippet: P . capsici colonies were grown on water agar supplemented with 1% (w / v) of listed sugars at 28°C for five days. Average growth (mm / day) rate is listed above each label. * Indicates significant differences in growth rate compared to water agar, α equal to 0.05.
Techniques Used: