phusion u hot start dna polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phusion u hot start dna polymerase
    Phusion U Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion u hot start dna polymerase/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phusion u hot start dna polymerase - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: .. Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

    Mutagenesis:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: .. Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

    Isolation:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Transformants were selected on carbenicillin LB agar, and isolated colonies were cultured overnight in 2 mL LB media.

    Construct:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

    Purification:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: .. Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

    Generated:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

    Cell Culture:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Transformants were selected on carbenicillin LB agar, and isolated colonies were cultured overnight in 2 mL LB media.

    Expressing:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: .. Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

    Sequencing:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Plasmids were extracted using a microcentrifuge membrane column kit (Miniprep, Qiagen), and the sequence of genes were confirmed by Sanger sequencing .

    Plasmid Preparation:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: .. Site-directed mutagenesis, expression, and purification of human IDE N-terminally His6 -tagged human IDE(42–1019) was cloned into the expression plasmid pTrcHis-A (Invitrogen) using primers for uracil-specific excision reactions (USER) and Phusion U Hot-Start DNA-polymerase (ThermoFisher F555S) . .. Mutant IDE constructs were generated by amplifying the complete pTrcHis-A-hIDE(42–1019) vector construct with USER cloning primers introducing a mutant overhang ( ) as previously described and introduced by heat shock into NEB turbo E. coli cells.

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  • 99
    Thermo Fisher phusion hot start dna polymerases
    A diagram of the Hot Fusion process for single fragment cloning. Red and blue boxes on the vector and PCR product indicate the overlapping sequences (17–30 bp). T5 exonuclease removes nucleotides from the 5′ to 3′ end of double strand <t>DNA</t> molecules. <t>Phusion</t> DNA polymerase fills in gaps that are over-generated by T5 exonuclease. Annealed fragments are transformed into E. coli and the nick sites in the nucleotide chain are repaired by E. coli.
    Phusion Hot Start Dna Polymerases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion hot start dna polymerases/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phusion hot start dna polymerases - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher phusion green hot start ii high fidelity dna polymerase
    A diagram of the Hot Fusion process for single fragment cloning. Red and blue boxes on the vector and PCR product indicate the overlapping sequences (17–30 bp). T5 exonuclease removes nucleotides from the 5′ to 3′ end of double strand <t>DNA</t> molecules. <t>Phusion</t> DNA polymerase fills in gaps that are over-generated by T5 exonuclease. Annealed fragments are transformed into E. coli and the nick sites in the nucleotide chain are repaired by E. coli.
    Phusion Green Hot Start Ii High Fidelity Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion green hot start ii high fidelity dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    phusion green hot start ii high fidelity dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    A diagram of the Hot Fusion process for single fragment cloning. Red and blue boxes on the vector and PCR product indicate the overlapping sequences (17–30 bp). T5 exonuclease removes nucleotides from the 5′ to 3′ end of double strand DNA molecules. Phusion DNA polymerase fills in gaps that are over-generated by T5 exonuclease. Annealed fragments are transformed into E. coli and the nick sites in the nucleotide chain are repaired by E. coli.

    Journal: PLoS ONE

    Article Title: Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

    doi: 10.1371/journal.pone.0115318

    Figure Lengend Snippet: A diagram of the Hot Fusion process for single fragment cloning. Red and blue boxes on the vector and PCR product indicate the overlapping sequences (17–30 bp). T5 exonuclease removes nucleotides from the 5′ to 3′ end of double strand DNA molecules. Phusion DNA polymerase fills in gaps that are over-generated by T5 exonuclease. Annealed fragments are transformed into E. coli and the nick sites in the nucleotide chain are repaired by E. coli.

    Article Snippet: Phusion Hot Start DNA polymerases and GeneRuler 1 kb Plus DNA Ladder were purchased from Thermo Scientific (Waltham, MA).

    Techniques: Clone Assay, Plasmid Preparation, Polymerase Chain Reaction, Generated, Transformation Assay