phusion polymerase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Phusion High Fidelity DNA Polymerase 2 U µL
    Description:
    Thermo Scientific Phusion High Fidelity DNA Polymerases set a gold standard for high performance PCR Featuring an error rate 50 fold lower than that of Taq and 6 fold lower than that of Pfu Phusion High Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity Phusion DNA Polymerases offer robust performance with short protocol times even in the presence of PCR inhibitors and generate higher yields with lower enzyme amounts than other DNA polymerase Highlights• High fidelity 52X Taq • Fast PCR due to short extension times 15 30 s kb • Robust performance minimal optimization needed• High yields of PCR products with minimal enzyme amounts• Available as a Green buffer format for direct loading of PCR products on gels F 534S or F 534L Applications• High fidelity PCR• Cloning• Template generation for sequencing• Amplification of difficult GC rich templates• Long range PCR up to 20 kb • Mutagenesis• High throughput PCR• MicroarrayUsing Phusion DNA Polymerases Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases such as Taq DNA polymerases For optimal results use our Tm calculator at www thermofisher com tmcalculator
    Catalog Number:
    f530l
    Price:
    None
    Applications:
    High Fidelity PCR|PCR|PCR & Real-Time PCR
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Thermo Scientific Phusion High Fidelity DNA Polymerases set a gold standard for high performance PCR Featuring an error rate 50 fold lower than that of Taq and 6 fold lower than that of Pfu Phusion High Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity Phusion DNA Polymerases offer robust performance with short protocol times even in the presence of PCR inhibitors and generate higher yields with lower enzyme amounts than other DNA polymerase Highlights• High fidelity 52X Taq • Fast PCR due to short extension times 15 30 s kb • Robust performance minimal optimization needed• High yields of PCR products with minimal enzyme amounts• Available as a Green buffer format for direct loading of PCR products on gels F 534S or F 534L Applications• High fidelity PCR• Cloning• Template generation for sequencing• Amplification of difficult GC rich templates• Long range PCR up to 20 kb • Mutagenesis• High throughput PCR• MicroarrayUsing Phusion DNA Polymerases Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases such as Taq DNA polymerases For optimal results use our Tm calculator at www thermofisher com tmcalculator
    https://www.bioz.com/result/phusion polymerase/product/Thermo Fisher
    Average 99 stars, based on 767 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes"

    Article Title: Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-13-1

    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) Phusion; (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Figure Legend Snippet: Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) Phusion; (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.

    Techniques Used: Polymerase Chain Reaction, Amplification, Positive Control

    2) Product Images from "Kinetics and Fidelity of Psychrophilic DNA Polymerases"

    Article Title: Kinetics and Fidelity of Psychrophilic DNA Polymerases

    Journal: bioRxiv

    doi: 10.1101/2020.08.04.236919

    (a) High-sensitivity Bioanalyzer DNA (Agilent) assay of replicated products after 20 or 26 rounds of PCR amplification in the flow-through (FT) or elution fractions of Ampure purification output. (b) Deletion and substitution rate (per base) is correlated as modeled by linear regression (r 2 : 0.98). (c) Substitution spectrum of mesophilic and thermophilic DNA polymerases across reaction temperature. (d) Multiple sequence alignment (Clustal Omega) of peptide sequences for polymerases used in this study. While Phusion DNA polymerase sequence is undisclosed, it is expected to be a derivative of Pyrococcus DNA polymerase II (Pfu). Family A cluster consists of Taq, PIPI, and Klenow polymerases. Family B polymerases consist of T4, Pfu, and PIPB.
    Figure Legend Snippet: (a) High-sensitivity Bioanalyzer DNA (Agilent) assay of replicated products after 20 or 26 rounds of PCR amplification in the flow-through (FT) or elution fractions of Ampure purification output. (b) Deletion and substitution rate (per base) is correlated as modeled by linear regression (r 2 : 0.98). (c) Substitution spectrum of mesophilic and thermophilic DNA polymerases across reaction temperature. (d) Multiple sequence alignment (Clustal Omega) of peptide sequences for polymerases used in this study. While Phusion DNA polymerase sequence is undisclosed, it is expected to be a derivative of Pyrococcus DNA polymerase II (Pfu). Family A cluster consists of Taq, PIPI, and Klenow polymerases. Family B polymerases consist of T4, Pfu, and PIPB.

    Techniques Used: Polymerase Chain Reaction, Amplification, Purification, Sequencing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
    Article Snippet: .. PCR conditions PCR with the following DNA polymerases: HotMaster™ Taq DNA Polymerase (Eppendorf), Platinum® Taq DNA Polymerase (Invitrogen), Pwo DNA Polymerase (Roche), Phusion™ DNA Polymerase (Finnzymes), and PfuTurbo® Cx Hotstart DNA polymerase (PfuCx) (Stratagene) was performed according to manufacturers' instructions on pBAD-TOPO® (Invitrogen) containing the gene, At5g43440 (Accession no. AY143873). ..

    Article Title: Production of in vitro amplified DNA pseudolibraries and high-throughput cDNA target amplification
    Article Snippet: .. Target amplifications When the novel Phusion DNA polymerase PCR mixture (Finnzymes) became available, we decided to compare its performance with that of the most successful PCR set up known to us. ..

    Article Title: Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results
    Article Snippet: .. Taq polymerase A range of 13 high fidelity, regular, economy and premium Taq polymerase enzymes were selected: Biotaq® (Bioline, London, UK), FastStart® High Fidelity PCR System (Roche, Mannheim, Germany), AmpliTaq Gold® (Applied Biosystems, Warrington, UK), HotStarTaq® DNA Polymerase (Qiagen, Hilden, Gernamy), Phusion® High Fidelity DNA Polymerase (Finnzymes, Espoo, Finland), Taq DNA Polymerase (Roche, Maylan, France), i-MaxTM II DNA Polymerase (iNtRON Biotechnology, Seongnam, Korea), KAPA HiFi™ (Kapa Biosystems, Boston, USA), OneTaq ™ DNA Polymerase (New England Biolabs, Hitchin, UK), Vent® DNA Polymerase (New England Biolabs, Hitchin, UK), Deep Vent® DNA Polymerase (New England Biolabs, Hitchin, UK), Pwo® DNA Polymerase (Roche, Maylan, France) and Velocity DNA Polymerase (Bioline, London, UK) (abbreviated names in ). .. Assessment of accuracy for different enzymesLoci for Tests 1–3 were amplified in a two-step process following the universal tailed amplicon design proposed by Roche .

    Article Title: Selection of efficient Taq DNA polymerase to optimize T-DNA genotyping method for rapid detection of mutant Arabidopsis thaliana plants
    Article Snippet: .. The following DNA polymerases were tested: Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific, USA, cat #F530S), Ex-Taq DNA polymerase (Clontech, USA, cat #RR001A), Taq DNA polymerase (SibEnzyme, Russia, cat #E331), Emerald Amp GT PCR Master Mix (Clontech, USA, cat #RR310A). .. 10 ng of genomic DNA and 10 μM of each primer were used in all PCR reactions; all other components of PCR reactions were added according to the manufacturer’s suggested protocol for each individual polymerase.

    Isolation:

    Article Title: Successful Establishment of Plasmids R1 and pMV158 in a New Host Requires the Relief of the Transcriptional Repression of Their Essential rep Genes
    Article Snippet: .. Determination of the relative amount of circular plasmid DNA in transformed pneumococcal cells by iPCR The plasmidic DNA (pDNA) present in the gDNA isolated from the transformed pneumococcal cells was used as template to perform an inverse PCR protocol with a primer set of divergent oligonucleotides. iPCR was performed using the Phusion High Fidelity (HF) (Thermo Scientific) DNA polymerase. .. Amplification reactions (20 μl) contained 0.7 ng of gDNA and 0.5 μM of the specific forward and reverse primers (Table ).

    Transformation Assay:

    Article Title: Successful Establishment of Plasmids R1 and pMV158 in a New Host Requires the Relief of the Transcriptional Repression of Their Essential rep Genes
    Article Snippet: .. Determination of the relative amount of circular plasmid DNA in transformed pneumococcal cells by iPCR The plasmidic DNA (pDNA) present in the gDNA isolated from the transformed pneumococcal cells was used as template to perform an inverse PCR protocol with a primer set of divergent oligonucleotides. iPCR was performed using the Phusion High Fidelity (HF) (Thermo Scientific) DNA polymerase. .. Amplification reactions (20 μl) contained 0.7 ng of gDNA and 0.5 μM of the specific forward and reverse primers (Table ).

    Inverse PCR:

    Article Title: Successful Establishment of Plasmids R1 and pMV158 in a New Host Requires the Relief of the Transcriptional Repression of Their Essential rep Genes
    Article Snippet: .. Determination of the relative amount of circular plasmid DNA in transformed pneumococcal cells by iPCR The plasmidic DNA (pDNA) present in the gDNA isolated from the transformed pneumococcal cells was used as template to perform an inverse PCR protocol with a primer set of divergent oligonucleotides. iPCR was performed using the Phusion High Fidelity (HF) (Thermo Scientific) DNA polymerase. .. Amplification reactions (20 μl) contained 0.7 ng of gDNA and 0.5 μM of the specific forward and reverse primers (Table ).

    Plasmid Preparation:

    Article Title: Successful Establishment of Plasmids R1 and pMV158 in a New Host Requires the Relief of the Transcriptional Repression of Their Essential rep Genes
    Article Snippet: .. Determination of the relative amount of circular plasmid DNA in transformed pneumococcal cells by iPCR The plasmidic DNA (pDNA) present in the gDNA isolated from the transformed pneumococcal cells was used as template to perform an inverse PCR protocol with a primer set of divergent oligonucleotides. iPCR was performed using the Phusion High Fidelity (HF) (Thermo Scientific) DNA polymerase. .. Amplification reactions (20 μl) contained 0.7 ng of gDNA and 0.5 μM of the specific forward and reverse primers (Table ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dna polymerases
    Genotyping of Arabidopsis thaliana plants from SALK_100012 line with (a) <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase; (b) Ex-Taq DNA polymerase; (c) Taq DNA polymerase; (d) Emerald Amp GT PCR Master Mix. WT - DNA from wild-type Arabidopsis plant (positive control for wild-type PCR band); 1–8 - individual plants from SALK_100012 line. Molecular weight DNA standards are shown on each side. Loading order for each DNA sample: PCR reaction for wild-type allele, PCR reaction for T-DNA mutant allele.
    Dna Polymerases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerases/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    dna polymerases - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    89
    Thermo Fisher phusion pcr
    Schematics of the subcycling <t>PCR</t> protocol. A. Each PCR cycle involves a denaturation step and an annealing/elongation step. We introduced 4X sub-cycling the annealing/elongation step within each of the 30X amplification cycles. B. Multiplexed amplification products for pools of 7 oligos with 154–200 bp length products of varying GC content. <t>Phusion</t> and KAPA HIFI polymerases were used with and without a sub-cycling thermocycle. Each different condition is used to amplify 12 separate oligo pools with GC content ranges as follows: 1.) 16.4–34.3; 2.) 13.5–38.7; 3.) 21.5–37.3; 4.) 12.2–12.2; 5.) 12.7–40.0; 6.) 14.9–41.6; 7.) 16.4–37.6; 8.) 12.7–42.0; 9.) 20.9–40.6; 10.) 12.5–42.5; 11.) 12.7–43.7; 12.) 14.8–35.6. Results are based on electronic gels created by electrophoresis using a Perkin Elmer GX instrument with a 5k chip. *Bin shows an example of an expected PCR pattern where a strong 154-200bp product band is seen. PCR reactions were not purified and primers can be seen at the bottom of each sample.
    Phusion Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion pcr/product/Thermo Fisher
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    phusion pcr - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    84
    Thermo Fisher phusion high fidelity dna polymeraseeach pcr amplification
    <t>PCR</t> performance of Herculase® II Fusion <t>DNA</t> polymerase and <t>Phusion™</t> High-Fidelity DNA Polymerase. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. 1, 2, 3 and 4: Herculase® II Fusion DNA polymerase; 5, 6, 7 and 8: Phusion™ High Fidelity PCR System; NC: negative control, respectively.
    Phusion High Fidelity Dna Polymeraseeach Pcr Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity dna polymeraseeach pcr amplification/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity dna polymeraseeach pcr amplification - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    Image Search Results


    Genotyping of Arabidopsis thaliana plants from SALK_100012 line with (a) Phusion High-Fidelity DNA polymerase; (b) Ex-Taq DNA polymerase; (c) Taq DNA polymerase; (d) Emerald Amp GT PCR Master Mix. WT - DNA from wild-type Arabidopsis plant (positive control for wild-type PCR band); 1–8 - individual plants from SALK_100012 line. Molecular weight DNA standards are shown on each side. Loading order for each DNA sample: PCR reaction for wild-type allele, PCR reaction for T-DNA mutant allele.

    Journal: BioNanoScience

    Article Title: Selection of efficient Taq DNA polymerase to optimize T-DNA genotyping method for rapid detection of mutant Arabidopsis thaliana plants

    doi: 10.1007/s12668-016-0253-6

    Figure Lengend Snippet: Genotyping of Arabidopsis thaliana plants from SALK_100012 line with (a) Phusion High-Fidelity DNA polymerase; (b) Ex-Taq DNA polymerase; (c) Taq DNA polymerase; (d) Emerald Amp GT PCR Master Mix. WT - DNA from wild-type Arabidopsis plant (positive control for wild-type PCR band); 1–8 - individual plants from SALK_100012 line. Molecular weight DNA standards are shown on each side. Loading order for each DNA sample: PCR reaction for wild-type allele, PCR reaction for T-DNA mutant allele.

    Article Snippet: The following DNA polymerases were tested: Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific, USA, cat #F530S), Ex-Taq DNA polymerase (Clontech, USA, cat #RR001A), Taq DNA polymerase (SibEnzyme, Russia, cat #E331), Emerald Amp GT PCR Master Mix (Clontech, USA, cat #RR310A).

    Techniques: Polymerase Chain Reaction, Positive Control, Molecular Weight, Mutagenesis

    Schematics of the subcycling PCR protocol. A. Each PCR cycle involves a denaturation step and an annealing/elongation step. We introduced 4X sub-cycling the annealing/elongation step within each of the 30X amplification cycles. B. Multiplexed amplification products for pools of 7 oligos with 154–200 bp length products of varying GC content. Phusion and KAPA HIFI polymerases were used with and without a sub-cycling thermocycle. Each different condition is used to amplify 12 separate oligo pools with GC content ranges as follows: 1.) 16.4–34.3; 2.) 13.5–38.7; 3.) 21.5–37.3; 4.) 12.2–12.2; 5.) 12.7–40.0; 6.) 14.9–41.6; 7.) 16.4–37.6; 8.) 12.7–42.0; 9.) 20.9–40.6; 10.) 12.5–42.5; 11.) 12.7–43.7; 12.) 14.8–35.6. Results are based on electronic gels created by electrophoresis using a Perkin Elmer GX instrument with a 5k chip. *Bin shows an example of an expected PCR pattern where a strong 154-200bp product band is seen. PCR reactions were not purified and primers can be seen at the bottom of each sample.

    Journal: PLoS ONE

    Article Title: Improved PCR Amplification of Broad Spectrum GC DNA Templates

    doi: 10.1371/journal.pone.0156478

    Figure Lengend Snippet: Schematics of the subcycling PCR protocol. A. Each PCR cycle involves a denaturation step and an annealing/elongation step. We introduced 4X sub-cycling the annealing/elongation step within each of the 30X amplification cycles. B. Multiplexed amplification products for pools of 7 oligos with 154–200 bp length products of varying GC content. Phusion and KAPA HIFI polymerases were used with and without a sub-cycling thermocycle. Each different condition is used to amplify 12 separate oligo pools with GC content ranges as follows: 1.) 16.4–34.3; 2.) 13.5–38.7; 3.) 21.5–37.3; 4.) 12.2–12.2; 5.) 12.7–40.0; 6.) 14.9–41.6; 7.) 16.4–37.6; 8.) 12.7–42.0; 9.) 20.9–40.6; 10.) 12.5–42.5; 11.) 12.7–43.7; 12.) 14.8–35.6. Results are based on electronic gels created by electrophoresis using a Perkin Elmer GX instrument with a 5k chip. *Bin shows an example of an expected PCR pattern where a strong 154-200bp product band is seen. PCR reactions were not purified and primers can be seen at the bottom of each sample.

    Article Snippet: For the Phusion PCR without additives we used 200μM dNTPs and a 1x final concentration of the Phusion 5x HF buffer from Thermo Fisher Scientific, Inc.

    Techniques: Polymerase Chain Reaction, Amplification, Electrophoresis, Chromatin Immunoprecipitation, Purification

    PCR performance of Herculase® II Fusion DNA polymerase and Phusion™ High-Fidelity DNA Polymerase. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. 1, 2, 3 and 4: Herculase® II Fusion DNA polymerase; 5, 6, 7 and 8: Phusion™ High Fidelity PCR System; NC: negative control, respectively.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of Herculase® II Fusion DNA polymerase and Phusion™ High-Fidelity DNA Polymerase. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. 1, 2, 3 and 4: Herculase® II Fusion DNA polymerase; 5, 6, 7 and 8: Phusion™ High Fidelity PCR System; NC: negative control, respectively.

    Article Snippet: Phusion™ High-Fidelity DNA polymeraseEach PCR amplification with Phusion™ High-Fidelity DNA Polymerase (Thermo Fisher Scientific, Waltham, MA) was carried out in a 25 μl of mixture containing Phusion™ High-Fidelity DNA Polymerase (2 units/μl) 0.25 μl, 5× reaction buffer 5 μl, dNTP (2.5 mM each) 0.5 μl, Template DNA 2 μl, forward primer 18S-CL-F3 (10 μm) 1.25 μl, reverse primer 28S-CL-R (10 μm) 1.25 μl, DMSO 0.25 μl, and molecular biology grade water (Sigma-Aldrich, St Louis, MO) 14.5 μl.

    Techniques: Polymerase Chain Reaction, Negative Control