phusion master mix  (New England Biolabs)


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  • 99
    Name:
    Phusion High Fidelity PCR Master Mix with GC Buffer
    Description:
    Phusion High Fidelity PCR Master Mix with GC Buffer 500 rxns 50 ul vol
    Catalog Number:
    M0532L
    Price:
    722
    Size:
    500 rxns
    Category:
    Thermostable DNA Polymerases
    Score:
    85
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    Structured Review

    New England Biolabs phusion master mix
    Phusion High Fidelity PCR Master Mix with GC Buffer
    Phusion High Fidelity PCR Master Mix with GC Buffer 500 rxns 50 ul vol
    https://www.bioz.com/result/phusion master mix/product/New England Biolabs
    Average 99 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    phusion master mix - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C.

    Amplification:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Size selection (100–350 bp) was done with Agencourt AMPure XP beads (Beckman Coulter Inc. (Analis SA) A63882) in combination with polyethylene glycol (PEG). .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). .. PCR products were purified with the High Pure PCR Cleanup Micro Kit (Roche Applied Science 04983912001).

    Article Title: A collection of rumen bacteriome data from 334 mid-lactation dairy cows
    Article Snippet: The PCR reaction solution consisted of 0.5 U of Taq polymerase (TransGen Biotech Co., Ltd., Beijing, China) in a 25 μl of 10 × PCR reaction Buffer, 200 μM of each dNTP, 0.2 μM of each primer and 2 μl of DNA. .. Thirty-five cycles PCR reactions were carried out using Phusion High-Fidelity PCR Master Mix (New England Biolabs Ltd., Ipswich, USA) with GC buffer and high efficiency-high fidelity enzyme to ensure the efficiency and accuracy of amplification , which was done with the following procedures: 1) at 94 °C for 3 min; 2) 35 cycles at 94 °C for 45 s, 50 °C for 60 s and 72 °C for 90 s; 3) final extension at 72 °C for 10 min. .. The PCR products were mixed with the same volume of 1 × loading buffer (contained SYBR safe) and conducted electrophoresis detection on 2% agarose gel (80 v, 40 min).

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The gene-specific primers pTortD5fls (5′-CGGGATCCGTCATGGGTTTTGTGACTCCACTTAAATGG-3′) and pTortD5fl2as (5′-GCGAATTCTTAGTGCTCTGAGCCTATTGGTGCAAACTG-3′) were designed manually in BioEdit (Hall ) to include the start/stop codon of the C. obliquana and C. herana desat7 ORFs, as well as a BamHI recognition site (sense primer) or an EcoRI recognition site (antisense primer). .. The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The products were ligated into pYEXCHT (Patel et al. ) with the CloneJET™ PCR Cloning Kit according to the instructions provided by the manufacturer (Thermo Scientific, Waltham, MA, USA), and cloned into One Shot® TOP10 Chemically Competent E. coli (Life Technologies).

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: Paragraph title: V4 region of the 16S rDNA PCR amplification ... The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O.

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: MmeI-digested gDNA and an Illumina barcoded adaptor with two random base pair overhangs were ligated using T4 DNA ligase (Epicentre) for 1 h at 25°C. .. The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor. .. The PCR was terminated during linear amplification ( ).

    Synthesized:

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The products were ligated into pYEXCHT (Patel et al. ) with the CloneJET™ PCR Cloning Kit according to the instructions provided by the manufacturer (Thermo Scientific, Waltham, MA, USA), and cloned into One Shot® TOP10 Chemically Competent E. coli (Life Technologies).

    Quantitative RT-PCR:

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: Paragraph title: RT-PCR, RT-qPCR, and Taqman qPCR ... RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated.

    Electrophoresis:

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O. .. The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O.

    Incubation:

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor. .. The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor.

    Transfection:

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: For analysis of transcript levels and splicing via RT-qPCR and RT-PCR, respectively, RNA was purified from transfected cells using the ZR RNA Miniprep Kit (Zymo). .. RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated.

    Gas Chromatography:

    Article Title: A collection of rumen bacteriome data from 334 mid-lactation dairy cows
    Article Snippet: The PCR reaction solution consisted of 0.5 U of Taq polymerase (TransGen Biotech Co., Ltd., Beijing, China) in a 25 μl of 10 × PCR reaction Buffer, 200 μM of each dNTP, 0.2 μM of each primer and 2 μl of DNA. .. Thirty-five cycles PCR reactions were carried out using Phusion High-Fidelity PCR Master Mix (New England Biolabs Ltd., Ipswich, USA) with GC buffer and high efficiency-high fidelity enzyme to ensure the efficiency and accuracy of amplification , which was done with the following procedures: 1) at 94 °C for 3 min; 2) 35 cycles at 94 °C for 45 s, 50 °C for 60 s and 72 °C for 90 s; 3) final extension at 72 °C for 10 min. .. The PCR products were mixed with the same volume of 1 × loading buffer (contained SYBR safe) and conducted electrophoresis detection on 2% agarose gel (80 v, 40 min).

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The gene-specific primers pTortD5fls (5′-CGGGATCCGTCATGGGTTTTGTGACTCCACTTAAATGG-3′) and pTortD5fl2as (5′-GCGAATTCTTAGTGCTCTGAGCCTATTGGTGCAAACTG-3′) were designed manually in BioEdit (Hall ) to include the start/stop codon of the C. obliquana and C. herana desat7 ORFs, as well as a BamHI recognition site (sense primer) or an EcoRI recognition site (antisense primer). .. The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The products were ligated into pYEXCHT (Patel et al. ) with the CloneJET™ PCR Cloning Kit according to the instructions provided by the manufacturer (Thermo Scientific, Waltham, MA, USA), and cloned into One Shot® TOP10 Chemically Competent E. coli (Life Technologies).

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: The variable V4 region of 16S rRNA was amplified by PCR using the following barcode primers: upstream primer 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and downstream primer 806R (5′-GGACTACHVGGGTWTCT AAT-3′). .. The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O. .. The amplification of PCR was performed as follows: 98 °C for 30 s; 30 cycles of 98 °C for 10 s, 54 °C for 30 s, and 72 °C for 30 s; and 72 °C for 7 min.

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: MmeI-digested gDNA and an Illumina barcoded adaptor with two random base pair overhangs were ligated using T4 DNA ligase (Epicentre) for 1 h at 25°C. .. The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor. .. The PCR was terminated during linear amplification ( ).

    Article Title: Microbiota Composition May Predict Anti-Tnf Alpha Response in Spondyloarthritis Patients: an Exploratory Study
    Article Snippet: The DNA was amplified using Master Mix Phusion GC Buffer (New England Biolabs® ). .. PCR conditions were as follows: 30 ng of DNA, two primers with final concentration 10 µM each, 25 µl of Master Mix Phusion GC Buffer, and completion with water leading to a final volume of 50 µl. .. Cycle conditions were as follows: 1 cycle of 98 °C, 30 s (hot start activation); 25 cycles of 98 °C,10 s (denaturation)/60 °C, 30 s (hybridation)/72 °C, 45 s (elongation); and 72 °C during 7 min (final elongation).

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: 1 μg of each RNA sample was treated with DNase I (Invitrogen) at 37°C for 1 h to remove DNA and then converted to cDNA with a SuperScript III reverse transcriptase kit (Invitrogen) according to manufacturer’s recommendations. .. RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated. .. RT-qPCR was carried out using Power SYBR Green Master Mix (ABI) on an Applied Biosystems Viia7 system in 96-well format under standard conditions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: 1 μg of each RNA sample was treated with DNase I (Invitrogen) at 37°C for 1 h to remove DNA and then converted to cDNA with a SuperScript III reverse transcriptase kit (Invitrogen) according to manufacturer’s recommendations. .. RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated. .. RT-qPCR was carried out using Power SYBR Green Master Mix (ABI) on an Applied Biosystems Viia7 system in 96-well format under standard conditions.

    Polymerase Chain Reaction:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Size selection (100–350 bp) was done with Agencourt AMPure XP beads (Beckman Coulter Inc. (Analis SA) A63882) in combination with polyethylene glycol (PEG). .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). .. PCR products were purified with the High Pure PCR Cleanup Micro Kit (Roche Applied Science 04983912001).

    Article Title: A collection of rumen bacteriome data from 334 mid-lactation dairy cows
    Article Snippet: The PCR reaction solution consisted of 0.5 U of Taq polymerase (TransGen Biotech Co., Ltd., Beijing, China) in a 25 μl of 10 × PCR reaction Buffer, 200 μM of each dNTP, 0.2 μM of each primer and 2 μl of DNA. .. Thirty-five cycles PCR reactions were carried out using Phusion High-Fidelity PCR Master Mix (New England Biolabs Ltd., Ipswich, USA) with GC buffer and high efficiency-high fidelity enzyme to ensure the efficiency and accuracy of amplification , which was done with the following procedures: 1) at 94 °C for 3 min; 2) 35 cycles at 94 °C for 45 s, 50 °C for 60 s and 72 °C for 90 s; 3) final extension at 72 °C for 10 min. .. The PCR products were mixed with the same volume of 1 × loading buffer (contained SYBR safe) and conducted electrophoresis detection on 2% agarose gel (80 v, 40 min).

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The gene-specific primers pTortD5fls (5′-CGGGATCCGTCATGGGTTTTGTGACTCCACTTAAATGG-3′) and pTortD5fl2as (5′-GCGAATTCTTAGTGCTCTGAGCCTATTGGTGCAAACTG-3′) were designed manually in BioEdit (Hall ) to include the start/stop codon of the C. obliquana and C. herana desat7 ORFs, as well as a BamHI recognition site (sense primer) or an EcoRI recognition site (antisense primer). .. The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The products were ligated into pYEXCHT (Patel et al. ) with the CloneJET™ PCR Cloning Kit according to the instructions provided by the manufacturer (Thermo Scientific, Waltham, MA, USA), and cloned into One Shot® TOP10 Chemically Competent E. coli (Life Technologies).

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: The variable V4 region of 16S rRNA was amplified by PCR using the following barcode primers: upstream primer 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and downstream primer 806R (5′-GGACTACHVGGGTWTCT AAT-3′). .. The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O. .. The amplification of PCR was performed as follows: 98 °C for 30 s; 30 cycles of 98 °C for 10 s, 54 °C for 30 s, and 72 °C for 30 s; and 72 °C for 7 min.

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: MmeI-digested gDNA and an Illumina barcoded adaptor with two random base pair overhangs were ligated using T4 DNA ligase (Epicentre) for 1 h at 25°C. .. The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor. .. The PCR was terminated during linear amplification ( ).

    Article Title: Microbiota Composition May Predict Anti-Tnf Alpha Response in Spondyloarthritis Patients: an Exploratory Study
    Article Snippet: The DNA was amplified using Master Mix Phusion GC Buffer (New England Biolabs® ). .. PCR conditions were as follows: 30 ng of DNA, two primers with final concentration 10 µM each, 25 µl of Master Mix Phusion GC Buffer, and completion with water leading to a final volume of 50 µl. .. Cycle conditions were as follows: 1 cycle of 98 °C, 30 s (hot start activation); 25 cycles of 98 °C,10 s (denaturation)/60 °C, 30 s (hybridation)/72 °C, 45 s (elongation); and 72 °C during 7 min (final elongation).

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated. .. Retrohoming of the Ll.LtrB intron in mammalian cells was assayed by Taqman qPCR using an Applied Biosystems Viia7 system in 384-well format using Taqman probes (Life Technologies).

    Binding Assay:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Paragraph title: Methyl-CpG binding domain protein sequencing ... 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina).

    Multiplexing:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Size selection (100–350 bp) was done with Agencourt AMPure XP beads (Beckman Coulter Inc. (Analis SA) A63882) in combination with polyethylene glycol (PEG). .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). .. PCR products were purified with the High Pure PCR Cleanup Micro Kit (Roche Applied Science 04983912001).

    Nucleic Acid Electrophoresis:

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. All ORFs were restricted from positive clones in a BamHI and EcoRI double digestion as instructed by the manufacturer (Promega).

    Methylation:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: After DNA fragmentation, the methylated fragments were captured using Diagenode’s MethylCapTM kit (Diagenode AF-100-0048) according to the manufacturer’s instructions starting from a DNA concentration between 250 and 500 ng. .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina).

    Size-exclusion Chromatography:

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The gene-specific primers pTortD5fls (5′-CGGGATCCGTCATGGGTTTTGTGACTCCACTTAAATGG-3′) and pTortD5fl2as (5′-GCGAATTCTTAGTGCTCTGAGCCTATTGGTGCAAACTG-3′) were designed manually in BioEdit (Hall ) to include the start/stop codon of the C. obliquana and C. herana desat7 ORFs, as well as a BamHI recognition site (sense primer) or an EcoRI recognition site (antisense primer). .. The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The products were ligated into pYEXCHT (Patel et al. ) with the CloneJET™ PCR Cloning Kit according to the instructions provided by the manufacturer (Thermo Scientific, Waltham, MA, USA), and cloned into One Shot® TOP10 Chemically Competent E. coli (Life Technologies).

    Purification:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Purified DNA was used for library preparation, which was performed on the Apollo 324TM (IntegenX) using the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina PE-400-1001). .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina).

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O. .. The amplified products were identified by electrophoresis on a 2% agarose gel containing ethidium bromide.

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor. .. The PCR was terminated during linear amplification ( ).

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: For analysis of transcript levels and splicing via RT-qPCR and RT-PCR, respectively, RNA was purified from transfected cells using the ZR RNA Miniprep Kit (Zymo). .. RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated.

    Sequencing:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Paragraph title: Methyl-CpG binding domain protein sequencing ... 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina).

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: MmeI-digested gDNA and an Illumina barcoded adaptor with two random base pair overhangs were ligated using T4 DNA ligase (Epicentre) for 1 h at 25°C. .. The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor. .. The PCR was terminated during linear amplification ( ).

    Sample Prep:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Size selection (100–350 bp) was done with Agencourt AMPure XP beads (Beckman Coulter Inc. (Analis SA) A63882) in combination with polyethylene glycol (PEG). .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). .. PCR products were purified with the High Pure PCR Cleanup Micro Kit (Roche Applied Science 04983912001).

    Chromatin Immunoprecipitation:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). .. PCR products were purified with the High Pure PCR Cleanup Micro Kit (Roche Applied Science 04983912001).

    Plasmid Preparation:

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The gene-specific primers pTortD5fls (5′-CGGGATCCGTCATGGGTTTTGTGACTCCACTTAAATGG-3′) and pTortD5fl2as (5′-GCGAATTCTTAGTGCTCTGAGCCTATTGGTGCAAACTG-3′) were designed manually in BioEdit (Hall ) to include the start/stop codon of the C. obliquana and C. herana desat7 ORFs, as well as a BamHI recognition site (sense primer) or an EcoRI recognition site (antisense primer). .. The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. The products were ligated into pYEXCHT (Patel et al. ) with the CloneJET™ PCR Cloning Kit according to the instructions provided by the manufacturer (Thermo Scientific, Waltham, MA, USA), and cloned into One Shot® TOP10 Chemically Competent E. coli (Life Technologies).

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated. .. RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated.

    Real-time Polymerase Chain Reaction:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). .. Next, libraries were assessed using an Agilent DNA 1000 Chip (Agilent Technologies 5067–1504).

    Article Title: Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
    Article Snippet: Paragraph title: RT-PCR, RT-qPCR, and Taqman qPCR ... RT-PCR was carried out with GC-rich Phusion polymerase mastermix (New England Biolabs) under standard conditions, unless otherwise indicated.

    Selection:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: Size selection (100–350 bp) was done with Agencourt AMPure XP beads (Beckman Coulter Inc. (Analis SA) A63882) in combination with polyethylene glycol (PEG). .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina).

    Agarose Gel Electrophoresis:

    Article Title: A Novel Fatty Acyl Desaturase from the Pheromone Glands of Ctenopseustis obliquana and C. herana with Specific Z5-Desaturase Activity on Myristic Acid
    Article Snippet: The full-length desat7 alleles named 26 and 38 from C. obliquana were PCR amplified from 100 ng cDNA or plasmid DNA using Phusion® High-Fidelity PCR Master Mix (New England Biolabs) with GC buffer under the following cycling conditions: 98 °C for 1 min, 98 °C 10 sec–60 °C 10 sec–72 °C 45 sec for 35 cycles, a final extension step at 72 °C for 1 min, and hold at 4 °C. .. All ORFs were restricted from positive clones in a BamHI and EcoRI double digestion as instructed by the manufacturer (Promega).

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O. .. The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O.

    Ethanol Precipitation:

    Article Title: Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides
    Article Snippet: Enzymes were inactivated at 65°C for 15 min, followed by phenol-chloroform–isoamyl acetate (25:24:1) extraction and ethanol precipitation at −20°C overnight. .. The transposon with 20 bp of genomic DNA sequence junction and ligated adaptor was amplified using Phusion High-Fidelity with GC buffer master mix (New England BioLabs) using primers (P1 M6 MmeI and Gex PCR Primer 2) that anneal to the inverted repeat of the transposon and the ligated Illumina adaptor.

    Concentration Assay:

    Article Title: A genome-wide search for eigenetically regulated genes in zebra finch using MethylCap-seq and RNA-seq
    Article Snippet: After DNA fragmentation, the methylated fragments were captured using Diagenode’s MethylCapTM kit (Diagenode AF-100-0048) according to the manufacturer’s instructions starting from a DNA concentration between 250 and 500 ng. .. 22 μl of DNA was subjected to PCR following the Illumina Library Amplification Index Protocol (Illumina) with 21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina).

    Article Title: Microbiota Composition May Predict Anti-Tnf Alpha Response in Spondyloarthritis Patients: an Exploratory Study
    Article Snippet: The DNA was amplified using Master Mix Phusion GC Buffer (New England Biolabs® ). .. PCR conditions were as follows: 30 ng of DNA, two primers with final concentration 10 µM each, 25 µl of Master Mix Phusion GC Buffer, and completion with water leading to a final volume of 50 µl. .. Cycle conditions were as follows: 1 cycle of 98 °C, 30 s (hot start activation); 25 cycles of 98 °C,10 s (denaturation)/60 °C, 30 s (hybridation)/72 °C, 45 s (elongation); and 72 °C during 7 min (final elongation).

    Gel Extraction:

    Article Title: Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
    Article Snippet: The PCR reaction mixture contained 12.5 μL of Phusion Master Mix (Phusion® High-Fidelity PCR Master Mix with GC Buffer; New England Biolabs, United States), 1 μL of template DNA, 1.25 μL of 10 μmol primer 515F, 1.25 μL of 10 μmol primer 806R and 10 μL of double-distilled H2 O. .. The amplified products were identified by electrophoresis on a 2% agarose gel containing ethidium bromide.

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    New England Biolabs phusion high fidelity master mix
    Phusion High Fidelity Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity master mix/product/New England Biolabs
    Average 99 stars, based on 339 article reviews
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    phusion high fidelity master mix - by Bioz Stars, 2020-01
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