phusion high fidelity dna polymerase  (New England Biolabs)


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    New England Biolabs phusion high fidelity dna polymerase
    Phusion High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity dna polymerase - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: .. In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304). .. The constructed RNA library was sequenced on an Illumina HiSeq 4000 system using 2×50 base pairs paired-end sequencing.

    Article Title: Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis
    Article Snippet: Paragraph title: 16S rRNA gene amplification and sequencing. ... For the PCR, each 20-µl reaction mixture consisted of 4 µl of 5 × Phusion HF buffer (M0530S; New England BioLabs Inc.), 1.6 µl of deoxynucleoside triphosphates (each at 2.5 µM), 1 µl of each primer (10 µM), 0.6 µl of dimethyl sulfoxide, 10 ng of template DNA, 0.2 µl of Phusion High-Fidelity DNA polymerase (0.4 U), and 10.6 µl of pure water.

    Article Title: A High-Resolution LC-MS-Based Secondary Metabolite Fingerprint Database of Marine Bacteria
    Article Snippet: The V3–V5 region of the 16S rRNA gene was amplified using the following pair of primers: 341F (5′-CCTACGGGAGGCAGCAG-3′) and 907R (5′-CCGTCAATTCCTTTRAGTTT-3′). .. Each 20 μL of PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of forward and reverse primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units), and 10.6 μL of pure water.

    Article Title: Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis
    Article Snippet: Paragraph title: PCR amplification of the 16S rRNA gene for pyrosequencing ... Each 20-μL PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of each primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units) and 10.6 μL of pure water.

    Agarose Gel Electrophoresis:

    Article Title: A High-Resolution LC-MS-Based Secondary Metabolite Fingerprint Database of Marine Bacteria
    Article Snippet: The quality and quantity of the DNA were checked using a NanoDrop ND-100 device (Thermo Fisher, USA) and by agarose gel electrophoresis. .. Each 20 μL of PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of forward and reverse primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units), and 10.6 μL of pure water.

    Article Title: Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis
    Article Snippet: Each 20-μL PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of each primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units) and 10.6 μL of pure water. .. The PCR products were detected by agarose gel electrophoresis, and the purified concentrations were measured using a NanoDrop ND-1000 device (Thermo Fisher, USA).

    Ligation:

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: .. Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use. .. Library quantification kit, Illumina/Universal (Kapa Biosystems, cat. no. KK4824) Sequencing kits, MiSeq reagent kit v2 (300 cycles; Illumina, cat. no. MS-102-2002) or 5× TruSeq rapid SBS kit—HS (50 cycle) and TruSeq rapid SR cluster kit—HS (Illumina, cat. nos.

    Isolation:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: Paragraph title: RNA isolation and sequencing ... In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304).

    Construct:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: Following extraction and purification, an RNA library was constructed using the NEBNext Ultra RNA Library Prep kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA) based on the manufacturer's instructions. .. In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304).

    Purification:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: .. In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304). .. The constructed RNA library was sequenced on an Illumina HiSeq 4000 system using 2×50 base pairs paired-end sequencing.

    Article Title: Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis
    Article Snippet: Each 20-μL PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of each primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units) and 10.6 μL of pure water. .. The PCR products were detected by agarose gel electrophoresis, and the purified concentrations were measured using a NanoDrop ND-1000 device (Thermo Fisher, USA).

    Polymerase Chain Reaction:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: .. In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304). .. The constructed RNA library was sequenced on an Illumina HiSeq 4000 system using 2×50 base pairs paired-end sequencing.

    Article Title: Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis
    Article Snippet: .. For the PCR, each 20-µl reaction mixture consisted of 4 µl of 5 × Phusion HF buffer (M0530S; New England BioLabs Inc.), 1.6 µl of deoxynucleoside triphosphates (each at 2.5 µM), 1 µl of each primer (10 µM), 0.6 µl of dimethyl sulfoxide, 10 ng of template DNA, 0.2 µl of Phusion High-Fidelity DNA polymerase (0.4 U), and 10.6 µl of pure water. .. A thermal cycler (Bio-Rad, USA) was used according to the following program: initial denaturation at 98°C for 1 min; 25 cycles of 98°C for 10 s, 60°C for 30 s, and 72°C for 20 s; and a final extension at 72°C for 5 min. Amplicons were mixed in equal amounts and pyrosequenced with a ROCHE 454 FLX Titanium platform.

    Article Title: A High-Resolution LC-MS-Based Secondary Metabolite Fingerprint Database of Marine Bacteria
    Article Snippet: .. Each 20 μL of PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of forward and reverse primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units), and 10.6 μL of pure water. .. The PCR was performed with a thermal cycler (Bio-Rad, USA) using the following program: an initial denaturation at 98°C for 1 min; 25 cycles at 98°C for 10 s, 60°C for 30 s and 72°C for 20 s; and a final extension at 72°C for 5 min. Sequences were assigned according to the Ribosomal Database Project (RDP) Classifier (version 2.2) of the QIIME pipeline using the Silva108 database with a confidence level of 80%.

    Article Title: Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis
    Article Snippet: .. Each 20-μL PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of each primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units) and 10.6 μL of pure water. .. PCR was performed with a thermal cycler (Bio-Rad, USA) using the following program: an initial denaturation at 98°C for 1 min; 25 cycles at 98°C for 10 s, 60°C for 30 s and 72°C for 20 s; and a final extension at 72°C for 5 min.

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: .. Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use. .. Library quantification kit, Illumina/Universal (Kapa Biosystems, cat. no. KK4824) Sequencing kits, MiSeq reagent kit v2 (300 cycles; Illumina, cat. no. MS-102-2002) or 5× TruSeq rapid SBS kit—HS (50 cycle) and TruSeq rapid SR cluster kit—HS (Illumina, cat. nos.

    Selection:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: .. In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304). .. The constructed RNA library was sequenced on an Illumina HiSeq 4000 system using 2×50 base pairs paired-end sequencing.

    Mass Spectrometry:

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use. .. Library quantification kit, Illumina/Universal (Kapa Biosystems, cat. no. KK4824) Sequencing kits, MiSeq reagent kit v2 (300 cycles; Illumina, cat. no. MS-102-2002) or 5× TruSeq rapid SBS kit—HS (50 cycle) and TruSeq rapid SR cluster kit—HS (Illumina, cat. nos.

    Sequencing:

    Article Title: Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis
    Article Snippet: Paragraph title: RNA isolation and sequencing ... In brief, four steps were performed: i) Total RNA (1 µg) was sheared into fragments (200–500 nucleotides) in the NEBNext First Strand Synthesis Reaction Buffer and the fragments were utilized to generate the double-stranded cDNA; ii) the cDNA was end-repaired and ligated with Illumina-specific adaptors; iii) size selection of the library was performed using 200 bp inserts to select suitable fragments for polymerase chain reaction (PCR) amplification; iv) PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs, cat. no. F-530S) and the products were purified with a QIAquick Nucleotide Removal kit (Qiagen, no. 28304).

    Article Title: Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis
    Article Snippet: Paragraph title: 16S rRNA gene amplification and sequencing. ... For the PCR, each 20-µl reaction mixture consisted of 4 µl of 5 × Phusion HF buffer (M0530S; New England BioLabs Inc.), 1.6 µl of deoxynucleoside triphosphates (each at 2.5 µM), 1 µl of each primer (10 µM), 0.6 µl of dimethyl sulfoxide, 10 ng of template DNA, 0.2 µl of Phusion High-Fidelity DNA polymerase (0.4 U), and 10.6 µl of pure water.

    Article Title: A High-Resolution LC-MS-Based Secondary Metabolite Fingerprint Database of Marine Bacteria
    Article Snippet: Paragraph title: 16s rRNA sequencing of culturable marine bacteria ... Each 20 μL of PCR reaction consisted of 4 μL of 5 × Phusion HF Buffer (M0530S New England BioLabs Inc.), 1.6 μL of dNTPs (2.5 μM each), 1 μL of forward and reverse primer (10 μM), 0.6 μL of DMSO, 10 ng of template DNA, 0.2 μL of Phusion® High-Fidelity DNA Polymerase (0.4 units), and 10.6 μL of pure water.

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use. .. Library quantification kit, Illumina/Universal (Kapa Biosystems, cat. no. KK4824) Sequencing kits, MiSeq reagent kit v2 (300 cycles; Illumina, cat. no. MS-102-2002) or 5× TruSeq rapid SBS kit—HS (50 cycle) and TruSeq rapid SR cluster kit—HS (Illumina, cat. nos.

    Hood:

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: Wear personal protective equipment and handle it in a fume hood. .. Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use.

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  • News
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  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs phusion high fidelity dna polymerase
    Phusion High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity dna polymerase/product/New England Biolabs
    Average 90 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity dna polymerase - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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