Review




Structured Review

Promega phrl-tk
Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/phrl+tk/pm40419121-70-11-12
Average 90 stars, based on 1 article reviews
phrl-tk - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Plasmid Preparation:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.

Negative Control:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.

Control:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.

Transfection:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.

Luciferase:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.

Reporter Assay:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.

Mutagenesis:

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4-EEPD1-ERK axis.
Article Snippet: HAECs were plated in a 24-well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL-TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: EEPD1 regulates inflammation and endothelial apoptosis in atherosclerosis through KLF4‐EEPD1‐ERK axis
Article Snippet: HAECs were plated in a 24‐well dish and simultaneously transfected with 0.25 μg of each plasmid along with 25 μg of phRL‐TK (Promega), which harbours the Renilla luciferase gene as an internal control for transfection efficiency, employing Lipofectamine 3000.

Article Title: Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Article Snippet: GRE2-Luc reporter ( ) and phRL-tk (Promega) with pcDNA-hGR were cotransfected into HEK293 cells using Lipofectamine 3000 (Invitrogen).

Article Title: Transcriptional regulation of tumor suppressor gene RASSF1A by HBx.
Article Snippet: The pGL3-basic vector (Promega) was used as the negative control, while phRL-tk (Promega) was used as the internal control.

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was co-transfected with the reporter plasmids.

Article Title: LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis
Article Snippet: Plasmids containing the luciferase reporter of wild-type (WT) or mutant (MUT) TUG1 were created in-house, and phRL-TK was used as an internal control (Promega, Madison, WI, USA).

Article Title: BDH1 reduces apoptosis and alleviates mitochondrial damage of cardiomyocytes under high glucose condition as a downstream target of miR-125b.
Article Snippet: Diabetes is a chronic metabolic disease, characterized prominently by a persistent elevation of blood glucose level beyond the normal range.. Prolonged hyperglycemia exerts deleterious effects on systems and organs of the body, leading to complications like diabetic cardiomyopathy (DCM).. Our study commenced by screening the gene 3-hydroxybutyrate dehydrogenase 1 (BDH1) with low expression in DCM via Gene Expression Omnibus (GEO) analysis (GSE123975).

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Article Snippet: To control for transfection efficiency, 10 ng of phRL-TK (Promega, Madison, WI, USA) was cotransfected with the reporter plasmids.



Similar Products

93
Addgene inc phrl tk
Phrl Tk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/phRL+TK+5BoxB+sp+36+(Plasmid+%23115365)/pm40946800-83-5-7
Average 93 stars, based on 1 article reviews
phrl tk - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

90
Promega phrl-tk
Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/phrl+tk/pm40419121-70-11-12
Average 90 stars, based on 1 article reviews
phrl-tk - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega phrl-tk-luc
Phrl Tk Luc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/phrl+tk+luc/pm40412002-204-31-34
Average 90 stars, based on 1 article reviews
phrl-tk-luc - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega phrl-tk-luc plasmids
Phrl Tk Luc Plasmids, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/pgl3+basic/pm40412002-142-3-6
Average 90 stars, based on 1 article reviews
phrl-tk-luc plasmids - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega renilla control plasmid phrl-tk
Renilla Control Plasmid Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/pgl3+basic/pm40344170-326-27-31
Average 90 stars, based on 1 article reviews
renilla control plasmid phrl-tk - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega phrl-tk plasmid
Phrl Tk Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk/phrl+tk+plasmid/pm39946769-144-1-3
Average 90 stars, based on 1 article reviews
phrl-tk plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results