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Cell Signaling Technology Inc phosphorylated iκb kinase ikk α β
HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
Phosphorylated Iκb Kinase Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated iκb kinase ikk α β/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
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phosphorylated iκb kinase ikk α β - by Bioz Stars, 2020-10
90/100 stars

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1) Product Images from "Immune-Enhancing Effects of a High Molecular Weight Fraction of Cynanchum wilfordii Hemsley in Macrophages and Immunosuppressed Mice"

Article Title: Immune-Enhancing Effects of a High Molecular Weight Fraction of Cynanchum wilfordii Hemsley in Macrophages and Immunosuppressed Mice

Journal: Nutrients

doi: 10.3390/nu8100600

HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
Figure Legend Snippet: HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

Techniques Used: Activation Assay, Isolation

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Article Title: Combined administration of a sedative dose sevoflurane and 60% oxygen reduces inflammatory responses to sepsis in animals and in human PMBCs
Article Snippet: .. Primary rabbit antibodies for nuclear factor-kappa B p65 subunit (p65), phosphorylated NF-κB p65 (p-p65), phosphorylated inhibitor of kappa B alpha (p-IκBα), and phosphorylated IκB kinase (IKK)-α/β (p-IKKα/β) (Cell Signalling Technology, Boston, USA) proteins were used to detect p65, p-p65, p-IκBα and p-IKKα/β protein expression. .. Then, the primary rabbit antibodies for proliferating cell nuclear antigen (PCNA), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), IKKα, IKKβ, and IκBα proteins (Cell Signaling Technology, Boston, USA) were used to detect PCNA, GAPDH, IKKα, IKKβ, and IκBα proteins, which were used as control.

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    Cell Signaling Technology Inc phosphorylated iκb kinase ikk α β
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Phosphorylated Iκb Kinase Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated iκb kinase ikk α β/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated iκb kinase ikk α β - by Bioz Stars, 2020-10
    90/100 stars
      Buy from Supplier

    85
    Cell Signaling Technology Inc anti phosphorylated iκb kinase ikk α β polyclonal antibodies
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Anti Phosphorylated Iκb Kinase Ikk α β Polyclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated iκb kinase ikk α β polyclonal antibodies/product/Cell Signaling Technology Inc
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    anti phosphorylated iκb kinase ikk α β polyclonal antibodies - by Bioz Stars, 2020-10
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    Cell Signaling Technology Inc rabbit polyclonal anti phospho iκb kinase ikk α β antibody
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Rabbit Polyclonal Anti Phospho Iκb Kinase Ikk α β Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti phospho iκb kinase ikk α β antibody - by Bioz Stars, 2020-10
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    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

    Journal: Nutrients

    Article Title: Immune-Enhancing Effects of a High Molecular Weight Fraction of Cynanchum wilfordii Hemsley in Macrophages and Immunosuppressed Mice

    doi: 10.3390/nu8100600

    Figure Lengend Snippet: HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

    Article Snippet: Antibodies against phosphorylated-inhibitor of kappa B (IκB)-α, phosphorylated-IκB kinase (IKK)-α/β, and phosphorylated-p38 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

    Techniques: Activation Assay, Isolation