phosphorylated glycogen synthase kinase 3 beta p gsk3β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3 beta p gsk3β
    Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin <t>and</t> <t>p-GSK3β</t> were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD
    Phosphorylated Glycogen Synthase Kinase 3 Beta P Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated glycogen synthase kinase 3 beta p gsk3β/product/Cell Signaling Technology Inc
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    1) Product Images from "High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin signaling pathway"

    Article Title: High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin signaling pathway

    Journal: Food Science and Biotechnology

    doi: 10.1007/s10068-020-00733-7

    Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin and p-GSK3β were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD
    Figure Legend Snippet: Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin and p-GSK3β were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

    phosphorylated glycogen synthase kinase 3 beta p gsk3β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3 beta p gsk3β
    Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin <t>and</t> <t>p-GSK3β</t> were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD
    Phosphorylated Glycogen Synthase Kinase 3 Beta P Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated glycogen synthase kinase 3 beta p gsk3β/product/Cell Signaling Technology Inc
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    phosphorylated glycogen synthase kinase 3 beta p gsk3β - by Bioz Stars, 2024-02
    96/100 stars

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    1) Product Images from "High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin signaling pathway"

    Article Title: High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin signaling pathway

    Journal: Food Science and Biotechnology

    doi: 10.1007/s10068-020-00733-7

    Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin and p-GSK3β were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD
    Figure Legend Snippet: Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin and p-GSK3β were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

    phosphorylated p glycogen synthase kinase 3 gsk3 β ser9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated p glycogen synthase kinase 3 gsk3 β ser9
    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Phosphorylated P Glycogen Synthase Kinase 3 Gsk3 β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "RFC3 induces epithelial-mesenchymal transition in lung adenocarcinoma cells through the Wnt/β-catenin pathway and possesses prognostic value in lung adenocarcinoma"

    Article Title: RFC3 induces epithelial-mesenchymal transition in lung adenocarcinoma cells through the Wnt/β-catenin pathway and possesses prognostic value in lung adenocarcinoma

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2019.4386

    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, p-GSK3-β (Ser9), GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Figure Legend Snippet: Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, p-GSK3-β (Ser9), GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.

    Techniques Used: Over Expression, Plasmid Preparation, Transfection, Western Blot, Expressing, Small Interfering RNA

    phosphorylation glycogen synthase kinase 3 beta p gsk3β  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylation glycogen synthase kinase 3 beta p gsk3β
    Phosphorylation Glycogen Synthase Kinase 3 Beta P Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphorylated glycogen synthase kinase 3 p gsk3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3 p gsk3
    Phosphorylated Glycogen Synthase Kinase 3 P Gsk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphorylated glycogen synthase kinase 3β p gsk3β ser 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3β p gsk3β ser 9
    Phosphorylated Glycogen Synthase Kinase 3β P Gsk3β Ser 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphorylated glycogen synthase kinase 3β p gsk3β ser 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3β p gsk3β ser 9
    Phosphorylated Glycogen Synthase Kinase 3β P Gsk3β Ser 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phosphorylated glycogen synthase kinase 3 p gsk3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phosphorylated glycogen synthase kinase 3 p gsk3
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    anti phosphorylated glycogen synthase kinase 3 p gsk3 s9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phosphorylated glycogen synthase kinase 3 p gsk3 s9
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    phosphorylated glycogen synthase kinase 3 p gsk3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3 p gsk3
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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3 beta p gsk3β
    Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin <t>and</t> <t>p-GSK3β</t> were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD
    Phosphorylated Glycogen Synthase Kinase 3 Beta P Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated glycogen synthase kinase 3 beta p gsk3β/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc phosphorylated p glycogen synthase kinase 3 gsk3 β ser9
    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Phosphorylated P Glycogen Synthase Kinase 3 Gsk3 β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phosphorylation glycogen synthase kinase 3 beta p gsk3β
    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Phosphorylation Glycogen Synthase Kinase 3 Beta P Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3 p gsk3
    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Phosphorylated Glycogen Synthase Kinase 3 P Gsk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Phosphorylated Glycogen Synthase Kinase 3β P Gsk3β Ser 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Anti Phosphorylated Glycogen Synthase Kinase 3 P Gsk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, <t>p-GSK3-β</t> <t>(Ser9),</t> GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.
    Anti Phosphorylated Glycogen Synthase Kinase 3 P Gsk3 S9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin and p-GSK3β were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD

    Journal: Food Science and Biotechnology

    Article Title: High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin signaling pathway

    doi: 10.1007/s10068-020-00733-7

    Figure Lengend Snippet: Effect of high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) on the Wnt/β-catenin pathway. (A) mRNA levels of LRP5, DVL2, β-catenin, and CCND1 were evaluated with RT-PCR. (B) Protein levels of β-catenin and p-GSK3β were evaluated with western blotting. (C) The effects of β-catenin siRNA on mRNA levels of β-catenin, CCND1, PPARγ, and C/EBPα in the presence and absence of SPW-HHPE were evaluated with RT-PCR. β-Actin and α-tubulin served as loading controls. * and **, significant difference from MDI control (p < 0.05 and p < 0.01, respectively). Data represent % of control and are shown as mean ± SD

    Article Snippet: PPARγ, phosphorylated glycogen synthase kinase 3 beta (p-GSK3β), and α-tubulin antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot

    Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, p-GSK3-β (Ser9), GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.

    Journal: International Journal of Molecular Medicine

    Article Title: RFC3 induces epithelial-mesenchymal transition in lung adenocarcinoma cells through the Wnt/β-catenin pathway and possesses prognostic value in lung adenocarcinoma

    doi: 10.3892/ijmm.2019.4386

    Figure Lengend Snippet: Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, p-GSK3-β (Ser9), GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. * P<0.05, ** P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.

    Article Snippet: PVDF membranes were blocked with 5% milk in TTBS for 1 h at room temperature, and were probed with primary antibodies against RFC3 (1:500; cat. no. sc-390293; Santa Cruz Biotechnology, Inc.), Vimentin (1:1,000, cat. no. 5741S; Cell Signaling Technology, Inc.), N-cadherin (1:1,000, cat. no. 13116S, Cell Signaling Technology, Inc.), E-cadherin (1:1,000, cat. no. 14472S; Cell Signaling Technology, Inc.), phosphorylated (p)-glycogen synthase kinase 3 (GSK3)-β (Ser9) (1:1,000, cat. no. 5558S; Cell Signaling Technology, Inc.), β-catenin (1:1,000, cat. no. 8480S, Cell Signaling Technology, Inc.), GSK3-β (1:1,000, cat. no. 12456S; Cell Signaling Technology, Inc.), c-MYC (1:1,000, cat. no. 13987S; Cell Signaling Technology, Inc.), Wnt1 (1:1,000, cat. no. 27935-1-AP; Proteintech Group, Inc.), and GAPDH (1:1,000, cat. no. 5174S; Cell Signaling Technology, Inc.) or actin (1:2,000, cat. no. 3700S; Cell Signaling Technology, Inc.) overnight at 4°C.

    Techniques: Over Expression, Plasmid Preparation, Transfection, Western Blot, Expressing, Small Interfering RNA