phosphorylated foxo1  (Cell Signaling Technology Inc)

Journal: Experimental and Therapeutic Medicine

Article Title: Enhanced SLC2A4 expression mitigates insulin resistance in gestational diabetes mellitus via the FoxO signaling pathway in vitro

doi: 10.3892/etm.2025.12956

Figure Lengend Snippet: Role of the FoxO signaling pathway in pc-SLC2A4-induced changes in HG-induced HTR-8/SVneo cells. (A) Screen of targeted pathways related to gestational diabetes mellitus and SLC2A4 (five common genes identified through the CTD/GeneCards/DisGeNet database) using Kyoto Encyclopedia of Genes and Genomes, suggesting a critical role for FoxO signaling pathway. (B) The expression levels of p-FoxO1, FoxO1, p-FoxO3a and FoxO3a were analyzed by western blotting, GAPDH was used as the internal control for the results. The expression of INS and INSR was analyzed by (C) reverse transcription-quantitative PCR and (D) western blotting. (E) Glucose uptake was detected by glucose uptake assay. (F) Cell viability was measured using a Cell Counting Kit-8 assay. *** P<0.001 vs. Control; ns P>0.05 vs. HG; ### P<0.001 vs. HG + pc-NC; $ P<0.05, $$ P<0.01 and $$$ P<0.001 vs. HG + pc-SLC2A4. SLC2A4, solute carrier family 2 member 4; HG, high glucose; ns, not significant; NC, negative control; OD, optical density; INS, insulin; INSR, INS receptor; p, phosphorylated; FoxO, Forkhead box O.

Article Snippet: The membranes were then incubated overnight at 4 ̊C with primary antibodies against SLC2A4 (cat. no. 2213S; Cell Signaling Technology, Inc.), INSR (cat. no. 3025S; Cell Signaling Technology, Inc.), INS (cat. no. ab181547; Abcam), phosphorylated (p)-FoxO1 (cat. no. 9461S; Cell Signaling Technology, Inc.), FoxO1 (cat. no. 2880S; Cell Signaling Technology, Inc.), p-FoxO3a (cat. no. 13129S; Cell Signaling Technology, Inc.), FoxO3a (cat. no. 12829S; Cell Signaling Technology, Inc.) and GAPDH (cat. no. 97166; Cell Signaling Technology, Inc.) (all 1:1,000 dilution).

Techniques: Expressing, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Counting, Negative Control

Journal: Preventive Nutrition and Food Science

Article Title: Standardized Fingerroot ( Boesenbergia pandurata ) Extract Decelerates the Development of Sarcopenia in Aged Rats

doi: 10.3746/pnf.2025.30.1.47

Figure Lengend Snippet: Effects of Boesenbergia pandurata extract (BPE) on protein-degradation-related factors. (A) MuRF1 and atrogin-1 mRNA expression. Values were normalized to the expression of β-actin. (B) p-FoxO3a and FoxO3a protein expression levels. Values were normalized to the expression of the total form of FoxO3a. β-Actin and α-tubulin were used as loading controls. Total RNA and protein were isolated from gastrocnemius muscle tissues. Values are presented as the mean±SD (n=4 per group). # P <0.05, ## P <0.01 (vs. Young group); * P <0.05, ** P <0.01 (vs. Aged group).

Article Snippet: After washing with in Tris-buffered saline containing Tween 20 (TBST), the membranes were incubated with primary antibodies against FoxO3a, phosphorylated (p)-FoxO3a Thr32, PI3K, p-PI3K Tyr458, Akt, p-Akt Ser473, mTOR, p-mTOR Ser2448, p70S6K, p-p70S6K Thr389, 4EBP1, p-4EBP1 Thr37/46, and α-tubulin (1:1,000 dilution; Cell Signaling) overnight at 4°C.

Techniques: Expressing, Isolation