phosphorylated extracellular signal regulated kinase phospho erk  (Cell Signaling Technology Inc)

 
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    Cell Signaling Technology Inc phosphorylated extracellular signal regulated kinase phospho erk
    Phosphorylated Extracellular Signal Regulated Kinase Phospho Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated extracellular signal regulated kinase phospho erk/product/Cell Signaling Technology Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosphorylated extracellular signal regulated kinase phospho erk - by Bioz Stars, 2020-09
    92/100 stars

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    Double Immunofluorescence Staining:

    Article Title: Contributions of p38 and ERK to the antinociceptive effects of TGF-β1 in chronic constriction injury-induced neuropathic rats
    Article Snippet: .. For double immunofluorescence staining of phosphorylated ERK (phospho-ERK) and microglia or astrocyte markers, spinal sections (10 μm) were incubated with a mixture of primary antibodies, anti-phospho-ERK antibodies (1:100, Thr202/Tyr204, cat. 9101; Cell Signaling Technology Inc.; polyclonal rabbit antibody) and anti-OX-42 (1:200) or anti-GFAP (1:200) antibodies, overnight at 4 °C. .. Spinal sections were then incubated with a mixture of Alexa Fluor 488-labeled chicken anti-mouse IgG antibody (1:400) and DyLight 549-conjugated donkey anti-rabbit IgG antibody (1:400) for 40 min at room temperature.

    Incubation:

    Article Title: Contributions of p38 and ERK to the antinociceptive effects of TGF-β1 in chronic constriction injury-induced neuropathic rats
    Article Snippet: .. For double immunofluorescence staining of phosphorylated ERK (phospho-ERK) and microglia or astrocyte markers, spinal sections (10 μm) were incubated with a mixture of primary antibodies, anti-phospho-ERK antibodies (1:100, Thr202/Tyr204, cat. 9101; Cell Signaling Technology Inc.; polyclonal rabbit antibody) and anti-OX-42 (1:200) or anti-GFAP (1:200) antibodies, overnight at 4 °C. .. Spinal sections were then incubated with a mixture of Alexa Fluor 488-labeled chicken anti-mouse IgG antibody (1:400) and DyLight 549-conjugated donkey anti-rabbit IgG antibody (1:400) for 40 min at room temperature.

    Article Title: Geranylgeranylacetone attenuates fibrogenic activity and induces apoptosis in cultured human hepatic stellate cells and reduces liver fibrosis in carbon tetrachloride-treated mice
    Article Snippet: .. After blocking with 5% milk, membranes were incubated with primary antibodies against SMA (Abcam, Cambridge, UK), β-actin, CHOP, Bip, ATF6, phosphorylated extracellular signal regulated kinase (phospho-ERK), eukaryotic initiation factor (eIF)2α, phospho-eIF2α, c-Jun N-terminal kinase (JNK), phospho-JNK, p38 mitogen-activated protein kinase (MAPK), phospho-p38MAPK, poly(ADP-ribose) polymerase (PARP), cleaved-PARP, Akt, phospho-Akt, HSP70 (Cell Signaling technology, Beverly, MA, USA) and type 1 collagen (Santa Cruz Biotechnology, Dallas, TX, USA). .. Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody.

    Blocking Assay:

    Article Title: Geranylgeranylacetone attenuates fibrogenic activity and induces apoptosis in cultured human hepatic stellate cells and reduces liver fibrosis in carbon tetrachloride-treated mice
    Article Snippet: .. After blocking with 5% milk, membranes were incubated with primary antibodies against SMA (Abcam, Cambridge, UK), β-actin, CHOP, Bip, ATF6, phosphorylated extracellular signal regulated kinase (phospho-ERK), eukaryotic initiation factor (eIF)2α, phospho-eIF2α, c-Jun N-terminal kinase (JNK), phospho-JNK, p38 mitogen-activated protein kinase (MAPK), phospho-p38MAPK, poly(ADP-ribose) polymerase (PARP), cleaved-PARP, Akt, phospho-Akt, HSP70 (Cell Signaling technology, Beverly, MA, USA) and type 1 collagen (Santa Cruz Biotechnology, Dallas, TX, USA). .. Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody.

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    Cell Signaling Technology Inc phosphorylated extracellular signal regulated kinase phospho erk
    Phosphorylated Extracellular Signal Regulated Kinase Phospho Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated extracellular signal regulated kinase phospho erk/product/Cell Signaling Technology Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosphorylated extracellular signal regulated kinase phospho erk - by Bioz Stars, 2020-09
    92/100 stars
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